Specific detection of Ralstonia solanacearum 16S rRNA sequences by AmpliDet RNA

European Journal of Plant Pathology

Volumn 110, Issue 1, 2004, Pages 25-33

  Van Der Wolf, J M ^a   Van Beckhoven, J R C M ^a   De Haan, E G ^b   Van Den Bovenkamp, G W ^b   Leone, G O M ^a  

^a WAGENINGEN UNIVERSITY   (Netherlands)

^b NAK   (Netherlands)

Author keywords

Bacterial wilt; Brown rot; Immunofluorescence cell staining; Molecular beacons; NASBA; RNA extraction

Indexed keywords

BACTERIAL DISEASE; BACTERIUM; DETECTION METHOD;

BACTERIA (MICROORGANISMS); FUNGI; MYXOGASTRIA; RALSTONIA; RALSTONIA SOLANACEARUM; RALSTONIA SYZYGII; SOLANUM TUBEROSUM; VISCUM; VISCUM ALBUM;

EID: 0942267961     PISSN: 09291873     EISSN: None     Source Type: Journal    
DOI: 10.1023/B:EJPP.0000010140.44017.1f     Document Type: Article

References (30)

1. Bentsink L, Leone GOM, Van Beckhoven JRCM, Van Schijndel H, Gemen B and Van der Wolf JM (2002) Amplification of RNA by NASBA allows direct detection of viable cells of Ralstonia solanacearum in potato. Journal of Applied Microbiology 93: 647-655
2. Boom R, Sol CJA, Salimans MMM, Jansen CL, Wertheim-van Dillen PME and Van der Noordaa J (1990) Rapid and simple method for purification of nucleic acids. Journal of Clinical Microbiology 28: 495-503
3. Boudazin G, Le Roux AC, Josi K, Labarre P and Jouan B (1999) Design of division specific primers of Ralstonia solanacearum and application to the identification of European isolates. European Journal of Plant Pathology 105: 373-380
4. Demeke T and Adams RP (1992) The effects of plant polysaccharides and buffer additives on PCR. BioTechniques 12: 332-334
5. Elphinstone JG (1996) Survival and possibilities for extinction of Pseudomonas solanacearum (Smith) Smith in cool climates. Potato Research 39: 403-410
6. Elphinstone JG, Hennessy J, Wilson JK and Stead D (1996) Sensitivity of different methods for the detection of Ralstonia solanacearum in potato tuber extracts. Bulletin OEPP/EPPO Bulletin 26: 663-678
7. Engelbrecht MC (1994) Modification of a selective medium for the isolation and quantification of Pseudomonas solanacearum. ACIAR Bacterial Newsletter 10: 3-5
8. Griep RA, Van Twisk C, Van Beckhoven JRCM, Van der Wolf JM and Schots A (1998) Development of specific recombinant monoclonal antibodies against the lipopolysaccharides of Ralstonia solanacearum race 3. Phytopathology 88: 795-803
9. Hayward AC (1991) Biology and epidemiology of bacterial wilt caused by Pseudomonas solanacearum. Annual review of Phytopathology 29: 65-87
10. Higuchi R, Dollinger G, Walsh PS and Griffith R (1992) Simultaneous amplification and detection of specific DNA sequences. Bio/Technology 10: 413-417
11. Hahn D, Amann RI, Ludwig W, Akkermans ADL and Schleifer KH (1992) Detection of micro-organisms in soil after in situ hybridization with rRNA-targeted, fluorescently labelled oligonucleotides. Journal of General Microbiology 138: 879-887
12. Holland PM, Abramson RD, Watson R and Gelfand DH (1991) Detection of specific polymerase chain reaction product by utilizing the 5′- >3′ exonuclease activity of Thermus aquaticus DNA polymerase. Proceedings of the National Academy of Sciences of the United States of America 88: 7276-7280
13. Janse JD (1988) A detection method for Pseudomonas solanacearum in symptomless potato tubers and some data on its sensitivity and specificity. Bulletin OEPP/EPPO Bulletin 18: 343-351
14. Kwok S and Higuchi R (1989) Avoiding false positives with PCR. Nature 339: 237-238
15. Leone G, van Schijndel H, van Gemen B, Kramer FR and Schoen CD (1998) Molecular beacon probes combined with amplification by NASBA enable homogeneous, real-time detection of RNA. Nucleic Acids Research 26: 2150-2155
16. Li X, Dorsch M, Del Dot T, Sly LI, Stackebrandt E and Hayward AC (1993) Phylogenetic studies of the rRNA group II pseudomonads based on 16S rRNA gene sequences. Journal of Applied Bacteriology 74: 837-842
17. Livak KJ, Flood SJA, Marmaro J, Giusti W and Eetz K (1995) Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridization. PCR Methods Application 4: 357-362
18. Rossen L, Norskøv P, Holmstøm K and Rasmussen OF (1992) Inhibition of PCR by components of food samples, microbial diagnostic assays and DNA-extraction solutions. International Journal of Food Microbiology 17: 37-45
19. Seal SE, Jackson LA, Young JPW and Daniels MJ (1993) Differentiation of Pseudomonas solanacearum, Pseudomonas syzygii, Pseudomonas pickettii and the blood disease bacterium by partial 16S rRNA sequencing: Construction of oligonucleotide primers for sensitive detection by polymerase chain reaction. Journal of General Microbiology 139: 1587-1594
20. Seal SE and Elphinstone JG (1994) Advances in identification and detection of Pseudomonas solanacearum. In: Hayward AC and Hartman GL (eds) Bacterial Wilt: The Disease and its Causative Agent, Pseudomonas solanacearum (pp 35-38) CAB International, Wallingford, Oxon, UK
21. Szemes M, Schoen CD and Van der Wolf JM (2001) Molecular beacons for homogeneous real time monitoring of amplification products Section 2.3.2. In: Akkermans ADL, Van Elsas JD and De Bruijn FJ (eds) Molecular Microbial Ecology Manual (pp 1-17) Kluwer Academic Publishers, the Netherlands
22. Tyagi S and Kramer FR (1996) Molecular beacons: Probes that fluoresce upon hybridization. Nature Biotechnology 14: 303-308
23. Van Beckhoven JRCM, Stead DE and Van der Wolf JM (2002) Detection of Clavibacter michiganensis subsp. sepedonicus by AmpliDet RNA, a new technology based on real time monitoring of NASBA amplicons with a molecular beacon. Journal of Applied Microbiology 93: 840-849
24. Van der Vliet GME, Schepers P, Schukkink RAF, van Gemen B and Klatser PR (1994) Assessment of mycobacterial viability by RNA amplification. Antimicrobial Agents and Chemotherapy 38: 1959-1965
25. Verwoerd TC, Dekker BMM and Hoekema A (1989) A small-scale procedure for the rapid isolation of plant RNAs. Nucleic Acid Research 17: 2362
26. Vet JAM, Majithia AR, Marras SAE, Tyagi S, Dube S, Poiesz J and Kramer FR (1999) Multiplex detection of four pathogenic retroviruses using molecular beacons. Proceedings of the National Academy of Sciences of the United States of America 96: 6394-6399
27. Weller SA, Elphinstone JG, Smith NC and Stead DE (2000a) Detection of Ralstonia solanacearum from potato tissue by post-enrichment TaqMan PCR. Bulletin OEPP 30: 381-383
28. Weller SA, Elphinstone JG, Smith NC, Boonham N and Stead DE (2000b) Detection of Ralstonia solanacearum strains with a quantitative, multiplex, real-time fluorogenic PCR (TaqMan) assay. Applied and Environmental Microbiology 66: 2853-2858
29. Wenneker M, Verdel MSW, Groeneveld RMW, Kempenaar C, Van Beuningen AR and Janse JD (1999) Ralstonia (Pseudomonas) solanacearum race 3 (biovar 2) in surface water and natural weed hosts: First report on stinging nettle (Urtica dioica). European Journal of Plant Pathology 105: 307-315
30. Wittwer C, Ririe K, Andrew R, David D, Gunry R and Balis U (1997) The LightCycler™: A microvolume multisample fluorimeter with rapid temperature control. BioTechniques 22: 176-181