In vitro modulation of preservative toxicity: High molecular weight hyaluronan decreases apoptosis and oxidative stress induced by benzalkonium chloride

European Journal of Pharmaceutical Sciences

Volumn 34, Issue 4-5, 2008, Pages 263-273

  Pauloin, Thierry ^a   Dutot, Mélody ^a   Warnet, Jean Michel ^a,b,c   Rat, Patrice ^a,b,c  

^a Centre Universitaire des Saints Pères   (France)

^b CENTRE DE RECHERCHE DES CORDELIERS   (France)

^c CENTRE HOSPITALIER NATIONAL D OPHTALMOLOGIE DES QUINZE VINGTS   (France)

Author keywords

Apoptosis; Benzalkonium chloride; Hyaluronan; Oxidative stress; P2X7 cell death receptor; Preservative

Indexed keywords

BENZALKONIUM CHLORIDE; BIOPOLYMER; DEATH RECEPTOR; DNA FRAGMENT; HERMES ANTIGEN; HYALURONIC ACID; PRESERVATIVE;

APOPTOSIS; ARTICLE; CELL MEMBRANE PERMEABILITY; CELL STRUCTURE; CELL VIABILITY; CHROMATIN CONDENSATION; CONFOCAL MICROSCOPY; CONTROLLED STUDY; CYTOFLUOROMETRY; CYTOSKELETON; CYTOTOXICITY; FLOW CYTOMETRY; FLUORESCENCE MICROSCOPY; GENE EXPRESSION; HUMAN; HUMAN CELL; IN VITRO STUDY; MICROPLATE CYTOFLUOROMETRY; MITOCHONDRION; MOLECULAR WEIGHT; OXIDATIVE STRESS; PRIORITY JOURNAL;

ACTINS; ANTIGENS, CD44; APOPTOSIS; BENZALKONIUM COMPOUNDS; CELL LINE; CELL MEMBRANE PERMEABILITY; CHROMATIN ASSEMBLY AND DISASSEMBLY; CYTOPROTECTION; CYTOSKELETON; DNA FRAGMENTATION; EPITHELIAL CELLS; HUMANS; HYALURONIC ACID; MITOCHONDRIA; MOLECULAR WEIGHT; NECROSIS; OXIDATIVE STRESS; PRESERVATIVES, PHARMACEUTICAL; PROTECTIVE AGENTS; TIME FACTORS;

EID: 47249088131     PISSN: 09280987     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ejps.2008.04.006     Document Type: Article

References (42)

1. Araki-Sasaki K., Ohashi Y., Sasabe T., Hayashi K., Watanabe H., Tano Y., and Handa H. An SV40-immortalized human corneal epithelial cell line and its characterization. Invest. Ophthalmol. Vis. Sci. 36 (1995) 614-621
2. Arnitz R., Ott H.W., Gstottner M., Nagl M., Scholtz A.W., and Neher A. A novel N-chlorotaurine-corticosteroid combination as a preservative-free local disinfectant: influence on the ciliary beat frequency in vitro. Acta Otolaryngol. 126 (2006) 291-294
3. Batts A.H., Marriott C., Martin G.P., Wood C.F., and Bond S.W. The effect of some preservatives used in nasal preparations on the mucus and ciliary components of mucociliary clearance. J. Pharm. Pharmacol. 42 (1990) 145-151
4. Baudouin C., Pisella P.J., Fillacier K., Goldschild M., Becquet F., De Saint J.M., and Bechetoille A. Ocular surface inflammatory changes induced by topical antiglaucoma drugs: human and animal studies. Ophthalmology 106 (1999) 556-563
5. Belloc F., Dumain P., Boisseau M.R., Jalloustre C., Reiffers J., Bernard P., and Lacombe F. A flow cytometric method using Hoechst 33342 and propidium iodide for simultaneous cell cycle analysis and apoptosis determination in unfixed cells. Cytometry 17 (1994) 59-65
6. Berg O.H., Lie K., and Steinsvag S.K. The effects of topical nasal steroids on rat respiratory mucosa in vivo, with special reference to benzalkonium chloride. Allergy 52 (1997) 627-632
7. Boek W.M., Romeijn S.G., Graamans K., Verhoef J.C., Merkus F.W., and Huizing E.H. Validation of animal experiments on ciliary function in vitro. II. The influence of absorption enhancers, preservatives and physiologic saline. Acta Otolaryngol. 119 (1999) 98-101
8. Borenfreund E., and Puerner J.A. Toxicity determined in vitro by morphological alterations and neutral red absorption. Toxicol. Lett. 24 (1985) 119-124
9. Budagian V., Bulanova E., Brovko L., Orinska Z., Fayad R., Paus R., and Bulfone-Paus S. Signaling through P2X7 receptor in human T cells involved p56lck, MAP kinases, and transcription factor AP-1 and NF-kappa B. J. Biol. Chem. 278 (2003) 1549-1560
10. Chen W.Y., and Abatangelo G. Functions of hyaluronan in wound repair. Wound Repair Regen. 7 (1999) 79-89
11. Choucroun P., Gillet D., Dorange G., Sawicki B., and Dewitte J.D. Comet assay and early apoptosis. Mutat. Res. 478 (2001) 89-96
12. Cureoglu S., Akkus M., Osma U., Yaldiz M., Oktay F., Can B., Guven C., Tekin M., and Meric F. The effect of benzalkonium chloride on rabbit nasal mucosa in vivo: an electron microscopy study. Eur. Arch. Otorhinolaryngol. 259 (2002) 362-364
13. de Fries R., and Mitsuhashi M. Quantification of mitogen induced human lymphocyte proliferation: comparison of Alamar Blue assay to 3H-thymidine incorporation assay. J. Clin. Lab. Anal. 9 (1995) 89-95
14. De Saint Jean M., Debbasch C., Brignole F., Warnet J.M., and Baudouin C. Relationship between in vitro toxicity of benzalkonium chloride and preservative-induced dry eye. Adv. Exp. Med. Biol. 506 (2002) 697-702
15. Debbasch C., De Saint Jean M., Pisella P.J., Rat P., Warnet J.M., and Baudouin C. Quaternary ammonium cytotoxicity in a human conjunctival cell line. J. Fr. Ophtalmol. 22 (1999) 950-958
16. Debbasch C., Pisella P.J., De Saint J.M., Rat P., Warnet J.M., and Baudouin C. Mitochondrial activity and glutathione injury in apoptosis induced by unpreserved and preserved beta-blockers on Chang conjunctival cells. Invest. Ophthalmol. Vis. Sci. 42 (2001) 2525-2533
17. Donnelly-Roberts D.L., Namovic M.T., Faltynek C.R., and Jarvis M.F. Mitogen-activated protein kinase and caspase signaling pathways are required for P2X7 receptor (P2X7R)-induced pore formation in human THP-1 cells. J. Pharmacol. Exp. Ther. 308 (2004) 1053-1061
18. Dutot M., Warnet J.M., Baudouin C., and Rat P. Cytotoxicity of contact lens multipurpose solutions: role of oxidative stress, mitochondrial activity and P2X7 cell death receptor activation. Eur. J. Pharm. Sci. 33 (2008) 138-145
19. Eleftheriadis H., Cheong M., Sandeman S., Syam P.P., Brittain P., Klintworth G.K., Lloyd A., and Liu C. Corneal toxicity secondary to inadvertent use of benzalkonium chloride preserved viscoelastic material in cataract surgery. Br. J. Ophthalmol. 86 (2002) 299-305
20. Freeman B.A., and Crapo J.D. Biology of disease: free radicals and tissue injury. Lab. Invest. 47 (1982) 412-426
21. Gomes A., Fernandes E., and Lima J.L. Fluorescence probes used for detection of reactive species. J. Biochem. Biophys. Methods 397 (2005) 414-423
22. Horton M.R., McKee C.M., Bao C., Liao F., Farber J.M., Hodge-Dufour J., Pure E., Oliver B.L., Wright T.M., and Noble P.W. Hyaluronan fragments synergize with interferon-gamma to induce the C-X-C chemokines mig and interferon-inducible protein-10 in mouse macrophages. J. Biol. Chem. 273 (1998) 35088-35094
23. Horton M.R., Olman M.A., and Noble P.W. Hyaluronan fragments induce plasminogen activator inhibitor-1 and inhibit urokinase activity in mouse alveolar macrophages: a potential mechanism for impaired fibrinolytic activity in acute lung injury. Chest 116 (1999) 17S
24. Idziorek T., Estaquier J., De Bels F., and Ameisen J.C. YOPRO-1 permits cytofluorometric analysis of programmed cell death (apoptosis) without interfering with cell viability. J. Immunol. Methods 185 (1995) 249-258
25. Karbowski M., Kurono C., Wozniak M., Ostrowski M., Tearnishi M., Nishizawa Y., Usukura J., Soji T., and Wakabayashi T. Free radical-induced megamitochondria formation and apoptosis. Free Radic. Biol. Med. 26 (1999) 396-409
26. Larson E.M., Doughman D.J., Gregerson D.S., and Obritsch W.F. A new, simple, nonradioactive, nontoxic in vitro assay to monitor corneal endothelial cell viability. Invest. Ophthalmol. Vis. Sci. 38 (1997) 1929-1933
27. Laurent T.C., and Fraser J.R. Hyaluronan. FASEB J. 6 (1992) 2397-2404
28. Lebe E., Baka M., Yavasoglu A., Aktug H., Ates U., and Uyanikgil Y. Effects of preservatives in nasal formulations on the mucosal integrity: an electron microscopic study. Pharmacology 72 (2004) 113-120
29. Matsuhashi T., Liu X., Karbowski M., Wozniak M., Antosiewicz J., and Wakabayashi T. Role of free radicals in the mechanism of the hydrazine-induced formation of megamitochondria. Free Radic. Biol. Med. 23 (1997) 285-293
30. McKee C.M., Penno M.B., Cowman M., Burdick M.D., Strieter R.M., Bao C., and Noble P.W. Hyaluronan (HA) fragments induce chemokine gene expression in alveolar macrophages. The role of HA size and CD44. J. Clin. Invest. 98 (1996) 2403-2413
31. Noble P.W. Hyaluronan and its catabolic products in tissue injury and repair. Matrix Biol. 21 (2002) 25-29
32. Oiso N., Fukai K., and Ishii M. Irritant contact dermatitis from benzalkonium chloride in shampoo. Contact Dermatitis 52 (2005) 54
33. Polack F.M. Penetrating keratoplasty using MK stored corneas and Na Hyaluronate (Healon). Trans. Am. Ophthalmol. Soc. 80 (1982) 248-261
34. Rassendren F., Buell G.N., Viginio C., Collo G., North R.A., and Surprenant A. The permeabilizing ATP receptor, P2X7. Cloning and expression of a human cDNA. J. Biol. Chem. 272 (1997) 5482-5486
35. Surprenant A., Rassendren F., Kawashima E., North R.A., and Buell G.N. The cytolytic P2Z receptor for extracellular ATP identified as a P2X receptor (P2X7). Science 272 (1996) 735-738
36. Tammi R., MacCallum D., Hascall V.C., Pienimaki J.P., Hyttinen M., and Tammi M. Hyaluronan bound to CD44 on keratinocytes is displaced by hyaluronan decasaccharides and not hexasaccharides. J. Biol. Chem. 273 (1998) 28878-28888
37. Toole B.P. Hyaluronan: from extracellular glue to pericellular cue. Nat. Rev. Cancer 4 (2004) 528-539
38. Tripathi B.J., Tripathi R.C., and Kolli S.P. Cytotoxicity of ophthalmic preservatives on human corneal epithelium. Lens Eye Toxic. Res. 9 (1992) 361-375
39. Viginio C., MacKenzie A., North R.A., and Surprenant A. Kinetics of cell lysis, dye uptake and permeability changes in cells expressing the rat P2X7 receptor. J. Physiol. 519 (1999) 335-346
40. Wang C.M., Chand Y.Y., and Sun S.H. Activation of P2X7 purinoceptor-stimulated TGF-beta 1 mRNA expression involves PKC/MAPK signalling pathway in rat brain-derived type-2 astrocyte cell line, RBA-2. Cell Signal. 15 (2003) 1129-1137
41. Weissman B., and Meyer K. The structure of hyalobiuronic acid and of hyaluronic acid from umbilical cord. J. Am. Chem. Soc. 27 (1954) 1753-1757
42. Zhao H., Kalivendi S., Zhang H., Joseph J., Nithipatikom K., Vasquez-Vivar J., and Kalyanaraman B. Superoxide reacts with hydroethidine but forms a fluorescent product that is distinctly different from ethidium: potential implications in intracellular fluorescence detection of superoxide. Free Radic. Biol. Med. 34 (2003) 1359-1368