Acid-induced sweetness of neoculin is ascribed to its pH-dependent agonistic-antagonistic interaction with human sweet taste receptor

FASEB Journal

Volumn 22, Issue 7, 2008, Pages 2323-2330

  Nakajima, Ken Ichiro ^a   Morita, Yuji ^a   Koizumi, Ayako ^a   Asakura, Tomiko ^a   Terada, Tohru ^a   Ito, Keisuke ^a   Shimizu Ibuka, Akiko ^b   Maruyama, Jun Ichi ^a   Kitamoto, Katsuhiko ^a   Misaka, Takumi ^a   Abe, Keiko ^a  

^a UNIVERSITY OF TOKYO   (Japan)

^b TOKYO UNIVERSITY OF AGRICULTURE   (Japan)

Author keywords

G protein coupled receptors; Histidine; Sweet protein

Indexed keywords

PROTEIN NEOCULIN; SWEET PROTEIN;

ARTICLE; HUMAN; HUMAN CELL; IN VITRO STUDY; PH; PRIORITY JOURNAL; PROTEIN FUNCTION; PROTEIN PROTEIN INTERACTION; SWEETNESS; TASTE BUD;

ASPARTAME; CURCULIGO; HUMANS; HYDROGEN-ION CONCENTRATION; PLANT PROTEINS; SWEETENING AGENTS; TASTE; TASTE BUDS;

EID: 46749139846     PISSN: 08926638     EISSN: None     Source Type: Journal    
DOI: 10.1096/fj.07-100289     Document Type: Article

References (27)

1. Li, X., Staszewski, L., Xu, H., Durick, K., Zoller, M., and Adler, E. (2002) Human receptors for sweet and umami taste. Proc. Natl. Acad. Sci. U. S. A. 99, 4692-4696
2. Chandrashekar, J., Hoon, M. A., Ryba, N. J., and Zuker, C. S. (2006) The receptors and cells for mammalian taste. Nature 444, 288-294
3. Xu, H., Staszewski, L., Tang, H., Adler, E., Zoller, M., and Li, X. (2004) Different functional roles of T1R subunits in the heteromeric taste receptors. Proc. Natl. Acad. Sci. U. S. A. 101, 14258-14263
4. Cui, M., Jiang, P., Maillet, E., Max, M., Margolskee, R. F., and Osman, R. (2006) The heterodimeric sweet taste receptor has multiple potential ligand binding sites. Curr. Pharm. Des. 12, 4591-4600
5. Jang, H. J., Kokrashvili, Z., Theodorakis, M. J., Carlson, O. D., Kim, B. J., Zhou, J., Kim, H. H., Xu, X., Chan, S. L., Juhaszova, M., Bernier, M., Mosinger, B., Margolskee, R. F., and Egan, J. M. (2007) Gut-expressed gustducin and taste receptors regulate secretion of glucagon-like peptide-1. Proc. Natl. Acad. Sci. U. S. A. 104, 15069-15074
6. Yamashita, H., Theerasilp, S., Aiuchi, T., Nakaya, K., Nakamura, Y., and Kurihara, Y. (1990) Purification and complete amino acid sequence of a new type of sweet protein taste-modifying activity, curculin. J. Biol. Chem. 265, 15770-15775
7. Shirasuka, Y., Nakajima, K., Asakura, T., Yamashita, H., Yamamoto, A., Hata, S., Nagata, S., Abo, M., Sorimachi, H., and Abe, K. (2004) Neoculin as a new taste-modifying protein occurring in the fruit of Curculigo latifolia. Biosci. Biotechnol. Biochem. 68, 1403-1407
8. Nakajima, K., Asakura, T., Oike, H., Morita, Y., Shimizu-Ibuka, A., Misaka, T., Sorimachi, H., Arai, S., and Abe, K. (2006) Neoculin, a taste-modifying protein, is recognized by human sweet taste receptor. Neuroreport 17, 1241-1244
9. Shimizu-Ibuka, A., Morita, Y., Terada, T., Asakura, T., Nakajima, K., Iwata, S., Misaka, T., Sorimachi, H., Arai, S., and Abe, K. (2006) Crystal structure of neoculin: insights into its sweetness and taste-modifying activity. J. Mol. Biol. 359, 148-158
10. Nakajima, K., Asakura, T., Maruyama, J., Morita, Y., Oike, H., Shimizu-Ibuka, A., Misaka, T., Sorimachi, H., Arai, S., Kitamoto, K., and Abe, K. (2006) Extracellular production of neoculin, a sweet-tasting heterodimeric protein with taste-modifying activity, by Aspergillus oryzae. Appl. Environ. Microbiol. 72, 3716-3723
11. Jin, F. J., Watanabe, T., Juvvadi, P. R., Maruyama, J., Arioka, M., and Kitamoto, K. (2007) Double disruption of the proteinase genes, tppA and pepE, increases the production level of human lysozyme by Aspergillus oryzae. Appl. Microbiol. Biotechnol. 76, 1059-1068
12. Jiang, P., Ji, Q., Liu, Z., Snyder, L. A., Benard, L. M., Margolskee, R. F., and Max, M. (2004) The cysteine-rich region of T1R3 determines responses to intensely sweet proteins. J. Biol. Chem. 279, 45068-45075
13. Galindo-Cuspinera, V., Winnig, M., Bufe, B., Meyerhof, W., and Breslin, P. A. (2006) A TAS1R receptor-based explanation of sweet "water-taste. " Nature 441, 354-357
14. Case, D. A., Darden, T. A., Cheatham, III, T. E., Simmerling, C. L., Wang, J., Duke, R. E,. Luo, R., Merz, K. M., Wang, B., Pearlman, D. A., Crowley, M., Brozell, S., Tsui, V., Gohlke, H., Mongan, J., Hornak, V., Cui, G., Beroza, P., Schafmeister, C., Caldwell, J. W., Ross, W. S., and Kollman, P.A. (2004), AMBER 8, University of California, San Francisco, CA, USA
15. Wang, J., Cieplak, P., and Kollman, P. A. (2000) How well does a restrained electrostatic potential (RESP) model perform in calculating conformational energies of organic and biological molecules? J. Comput. Chem. 21, 1049-1074
16. Simmerling, C., Strockbine, B., and Roitberg, A. E. (2002) All-atom structure prediction and folding simulations of a stable protein. J. Am. Chem. Soc. 124, 11258-11259
17. Still, W. C., Tempczyk, A., Hawley, R. C., and Hendrickson, T. (1990) Semianalytical treatment of solvation for molecular mechanics and dynamics. J. Am. Chem. Soc. 112, 6127-6129
18. Onufriev, A., Bashford, D., and Case, D. A. (2004) Exploring protein native states and large-scale conformational changes with a modified generalized born model. Proteins 55, 383-394
19. Schiffman, S. S., Sattely-Miller, E. A., Graham, B. G., Bennett, J. L., Booth, B. J., Desai, N., and Bishay, I. (2000) Effect of temperature, pH, and ions on sweet taste. Physiol. Behav. 68, 469-481
20. Alberts, B. J., Lewis, A., Raff, J., Roberts, M., and Walter, K. P. (2002) Molecular Biology of the Cell, 4th Ed., pp. 62-64, Garland Science, Taylor and Francis Group, New York, NY, USA
21. Nie, Y., Vigues, S., Hobbs, J. R., Conn, G. L., and Munger, S. D. (2005) Distinct contributions of T1R2 and T1R3 taste receptor subunits to the detection of sweet stimuli. Curr. Biol. 15, 1948-1952
22. Nie, Y., Hobbs, J. R., Vigues, S., Olson, W. J., Conn, G. L., and Munger, S. D. (2006) Expression and purification of functional ligand-binding domains of T1R3 taste receptors. Chem. Senses 31, 505-513
23. Jiang, P., Cui, M., Zhao, B., Snyder, L. A., Benard, L. M., Osman, R., Max, M., and Margolskee, R. F. (2005) Identification of the cyclamate interaction site within the transmembrane domain of the human sweet taste receptor subunit T1R3. J. Biol. Chem. 280, 34296-34305
24. Winnig, M., Bufe, B., Kratochwil, N. A., Slack, J. P., and Meyerhof, W. (2007) The binding site for neohesperidin dihydrochalcone at the human sweet taste receptor. BMC Struct. Biol. 7, 66
25. Koizumi, A., Nakajima, K., Asakura, T., Morita, Y., Ito, K., Shmizu-Ibuka, A., Misaka, T., and Abe, K. (2007) Taste-modifying sweet protein, neoculin, is received at human T1R3 amino terminal domain. Biochem. Biophys. Res. Commun. 358, 585-589
26. Kurihara, Y. (1992) Characteristics of antisweet substances, sweet proteins, and sweetness-inducing proteins. Crit. Rev. Food Sci. Nutr. 32, 231-252
27. Ito, K., Asakura, T., Morita, Y., Nakajima, K., Koizumi, A., Shimizu-Ibuka, A., Masuda, K., Ishiguro, M., Terada, T., Maruyama, J., Kitamoto, K., Misaka, T., and Abe, K. (2007) Microbial production of sensory-active miraculin. Biochem. Biophys. Res. Commun. 360, 407-411