Cellular characteristics of primary and immortal canine embryonic fibroblast cells

Experimental and Molecular Medicine

Volumn 36, Issue 4, 2004, Pages 325-335

  You, Seungkwon ^a   Moon, Jai Hee ^a   Kim, Tae Kyung ^a   Kim, Sung Chan ^b,e   Kim, Jai Woo ^c   Yoon, Du Hak ^d   Kwak, Sungwook ^b   Hong, Ki Chang ^a   Choi, Yun Jaie ^b   Kim, Hyunggee ^a  

^a Korea University   (South Korea)

^b Seoul National University   (South Korea)

^c Yeungnam University   (South Korea)

^d National Institute of Animal Science   (South Korea)

^e UNIVERSITY OF TEXAS AT DALLAS   (United States)

Author keywords

Canine embryonic fibroblast; Immortalization; p16INK4a; p53; Telomerase

Indexed keywords

MESSENGER RNA; PROTEIN; PROTEIN P16; PROTEIN P53; TELOMERASE;

ANIMAL CELL; ARTICLE; ASSAY; CELL CYCLE; CELL GROWTH; CELL IMMORTALIZATION; DOG; EMBRYO; FIBROBLAST; HUMAN; HUMAN CELL; METHYLATION; NONHUMAN; PHENOTYPE; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN EXPRESSION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

ANIMALIA; CANIS FAMILIARIS; MURINAE;

EID: 4644301507     PISSN: 12263613     EISSN: 20926413     Source Type: Journal    
DOI: 10.1038/emm.2004.43     Document Type: Article

References (32)

1. Attardi LD, Reczek EE, Cosmas C, Demicco EG, McCurrach ME, Lowe SW, Jacks T. PERP, an apoptosis-associated target of p53, is a novel member of the PMP-22/gas3 family. Genes Dev 2000;14:704-14
2. Aviv A, Harley CB. How long should telomeres be?, Curr Hypertens Rep 2001;3:145-51
3. Barak Y, Juven T, Haffner R, Oren M. Mdm2 expression is induced by wild type p53 activity. EMBO J 1993;12:461-8
4. Bodnar AG, Ouellette M, Frolkis M, Holt SE, Shiu CP, Morin GB, Harley CB, Shay JW, Lichtsteiner S, Wright WE. Extension of life-span by introduction of telomerase into normal human cells. Science 1998;279:349-52
5. Campisi J, Replicative senescence and immortalization, in: Stein G, Baserga R, Giordano A, Denhardt D (Eds). In The Molecular Basis of Cell Cycle and Growth Control, Wiley-Liss, New York, 1999,348-73
6. Dimri GP, Lee X, Basile G, Acosta M, Scott G, Roskelley C, Medrano EE, Linskens M, Rubelj I, Pereira-Smith O, Peacocke M, Campisi J. A biomarker that identifies senescent human cells in culture and in aging skin in vivo. Proc Natl Acad Sci USA 1995;92:9363-7
7. Dyson N. The regulation of E2F by pRB-family protein. Genes Dev 1998;12:2245-62
8. El-Diery W, Tokino T, Velculescu V, Levy D, Parsons R, Trent J, Lin D, Mercer E, Kinzler K, Vogelstein B. WAF1, a potential mediator of p53 tumor suppression. Cell 1993; 75:817-25
9. Felsher DW, Bishop JM. Transient excess of MYC activity can elicit genomic instability and tumorigenesis. Proc Natl Acad Sci USA 1999;96:3940-4
10. Greider CW. Telomere length regulation. Annu Rev Biochem 1996;65:337-65
11. Harris CC. Human tissues and cells in carcinogenesis research. Cancer Res 1987;47:1-10
12. Hayflick L, Moorehead PS. The serial cultivation of human diploid cell strains. Exp Cell Res 1961;25:585-621
13. ARF. Cell 1997;91:649-59
14. Kim H, You S, Kim I-J, Foster LK, Farris J, Ambady S, Ponce de Leon FA, Foster DN. Alterations in p53 and E2F-1 function common to immortalized chicken embryo fibroblasts. Oncogene 2001;20:2671-82
15. INK4a and telomere regulating genes in the replicative senescent human, mouse and chicken fibroblast cells. Exp Cell Res 2002a;272:199-208
16. Kim H, Farris J, Christman SA, Kong BW, Foster LK, O'Grady SM, Foster DN. Events in the immortalizing process of primary human mammary epithelial cells by the catalytic subunit of human telomerase. Biochem J 2002b;365:765-72
17. Ko LJ, Prives C. p53: puzzle and paradigm. Genes Dev 1996;10:1054-72
18. Merlo A, Herman JG, Mao L, Lee DJ, Gabrielson E, Burger PC, Baylin SB, Sidransky D. 5′ CpG island methylation is associated with transcriptional silencing of the tumor suppressor p16/CDKN2/MTS1 in human cancers. Nat Med 1995;88:686-92
19. Miyashita T, Reed JC. Tumor suppressor p53 is a direct transcriptional activator of the human bax gene. Cell 1995; 80:293-9
20. Oda E, Ohki R, Murasawa H, Nemoto J, Shibue T, Yamashita T, Tokino T, Taniguchi T, Tanaka N. Noxa, a BH3-only member of the Bcl-2 family and candidate mediator of p53-induced apoptosis. Science 2000;288:1053-8
21. Sedivy JM. Can ends justify the means?: Telomeres and the mechanisms of replicative senescence and immortalization in mammalian cells. Proc Natl Acad Sci USA 1998;95:9078-81
22. Serrano M, Hannon G, Beach D. A new regulatory motif in cell-cycle control causing specific inhibition of cyclin D/CDK4. Nature 1993;366:704-7
23. INK4a. Exp Cell Res 1997;237:7-13
24. INK4a. Cell 1997;88: 593-602
25. Serrano M, Blasco MA. Putting the stress on senescence. Curr Opin Cell Biol 2001;13:748-53
26. Sherr CJ. Cancer Cell Cycles. Science 1996;274:1672-7
27. Sherr CJ, DePinho RA. Cellular senescence: mitotic clock or culture shock?. Cell 2000;102:407-10
28. Tao W, Levine AJ. ARF stabilizes p53 by blocking nucleocytoplasmic shuttling of Mdm2. Proc Natl Acad Sci USA 1999;96:6937-41
29. You S, Heo M, Moon J-H, Kim S-C, Kwak S, Yoon D-H, Jin D, Hong K-C, Foster DN, Choi Y-J, Kim H. Establishment of life-span extended bovine fibroblast cells carrying the characterization of primary cells. Mol Cells 2004; in press
30. Weber J, Taylor L, Roussel M, Sherr CJ, Bar-Sagi D. Nucleolar Arf sequesters Mdm2 and activates p53. Nat Cell Biol 1999;1:20-6
31. Woods DB, Vousden KH. Regulation of p53 function. Exp Cell Res 2001;264:56-66
32. Wu XW, Bayle JH, Olson D, Levine AJ. The p53 mdm-2 autoregulatory feedback loop. Genes Dev 1993;7:1126-32