The human CXC chemokine granulocyte chemotactic protein 2 (GCP-2)/CXCL6 possesses membrane-disrupting properties and is antibacterial

Antimicrobial Agents and Chemotherapy

Volumn 52, Issue 7, 2008, Pages 2599-2607

  Linge, Helena M ^a,d   Collin, Mattias ^a   Nordenfelt, Pontus ^a   Mörgelin, Matthias ^a   Malmsten, Martin ^c   Egesten, Arne ^b  

^a LUND UNIVERSITY   (Sweden)

^b LUND UNIVERSITY   (Sweden)

^c UPPSALA UNIVERSITY   (Sweden)

^d FEINSTEIN INSTITUTE FOR MEDICAL RESEARCH   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALPHA CHEMOKINE; CHEMOKINE; CHEMOKINE RECEPTOR CXCR1; CHEMOKINE RECEPTOR CXCR2; EPITHELIAL DERIVED NEUTROPHIL ACTIVATING FACTOR 78; GRANULOCYTE CHEMOTACTIC PROTEIN 2; LIPOPOLYSACCHARIDE; NAP 2 CXCL7 CHEMOKINE; PEPTIDE DERIVATIVE; SODIUM CHLORIDE;

ALPHA HELIX; AMINO TERMINAL SEQUENCE; ANGIOGENESIS; ANTIBACTERIAL ACTIVITY; ARTICLE; BACTERIAL STRAIN; BETA SHEET; CARBOXY TERMINAL SEQUENCE; CHEMOTAXIS; CONCENTRATION RESPONSE; CONTROLLED STUDY; EPITHELIUM CELL; ESCHERICHIA COLI; GENE EXPRESSION; GRAM NEGATIVE BACTERIUM; GRAM POSITIVE BACTERIUM; INFLAMMATION; LEUKOCYTE ACTIVATION; MACROPHAGE; MEMBRANE DAMAGE; MESENCHYME CELL; MINIMAL BACTERICIDAL CONCENTRATION; NONHUMAN; PRIORITY JOURNAL; PSEUDOMONAS AERUGINOSA; RECEPTOR BINDING; RECEPTOR UPREGULATION; STAPHYLOCOCCUS AUREUS; STREPTOCOCCUS DYSGALACTIAE EQUISIMILIS; STREPTOCOCCUS PYOGENES;

AMINO ACID SEQUENCE; ANTI-BACTERIAL AGENTS; ANTIMICROBIAL CATIONIC PEPTIDES; BETA-THROMBOGLOBULIN; BLOOD BACTERICIDAL ACTIVITY; CELL MEMBRANE; CHEMOKINE CXCL5; CHEMOKINE CXCL6; ESCHERICHIA COLI; GRAM-NEGATIVE BACTERIA; GRAM-POSITIVE BACTERIA; HUMANS; LIPOSOMES; MICROBIAL SENSITIVITY TESTS; MODELS, MOLECULAR; PROTEIN STRUCTURE, SECONDARY; RECOMBINANT PROTEINS; STAPHYLOCOCCUS AUREUS; STREPTOCOCCUS PYOGENES;

EID: 46249102418     PISSN: 00664804     EISSN: None     Source Type: Journal    
DOI: 10.1128/AAC.00028-08     Document Type: Article

References (29)

1. Baggiolini, M. 2001. Chemokincs in pathology and medicine. J. Intern. Med. 250:91-104.
2. Boman, H. G. 2003. Antibacterial peptides: basic facts and emerging concepts. J. Intern. Med. 254:197-215.
3. Chertov, O., D. F. Michiel, L. Xu, J. M. Wang, K. Tani, W. J. Murphy, D. L. Longo, D. D. Taub, and J. J. Oppenheim. 1996. Identification of defensin-1, defensin-2, and CAP37/azurocidin as T-cell chemoattractant proteins released from interleukin-8-stimulated neutrophils. J. Biol. Chem. 271:2935-2940.
4. Cole, A. M., T. Ganz, A. M. Liese, M. D. Burdick, L. Liu, and R. M. Strieter. 2001. IFN-inducible ELR-CXC chemokincs display defensin-like antimicrobial activity. J. Immunol. 167:623-627.
5. Egesten, A., M. Eliasson, H. M. Johansson, A. I. OHn, M. Mörgelin, A. Mueller, J. E. Pease, I. M. Frick, and L. Björck. 2007. The CXC chemokine MIG/CXCL9 is important in innate immunity against Streptococcus pyogenes. J. Infect. Dis. 195:684-693.
6. Fillmore, R. A., S. E. Nelson, R. N. Lausch, and J. E. Oakes. 2003. Differential regulation of ENA-78 and GCP-2 gene expression in human corneal keratocytes and epithelial cells. Investig. Ophthalmol. Vis. Sci. 44:3432-3437.
7. Frick, I. M., P. Åkesson, H. Herwald, M. Mörgelin, M. Malmsten, D. K. Nägler, and L. Björck. 2006. The contact system - a novel branch of innate immunity generating antibacterial peptides. EMBO J. 25:5569-5578.
8. Ganz, T. 2001. Antimicrobial proteins and peptides in host defense. Semin. Respir. Infect. 16:4-10.
9. Ganz, T. 2003. Defensins: antimicrobial peptides of innate immunity. Nat. Rev. Immunol. 3:710-720.
10. Gijsbers, K., M. Gouwy, S. Struyf, A. Wuyts, P. Proost, G. Opdenakker, F. Penninckx, N. Ectors, K. Geboes, and J. Van Damme. 2005. GCP-2/CXCL6 synergizes with other endothelial cell-derived chemokincs in neutrophil mobilization and is associated with angiogenesis in gastrointestinal tumors. Exp. Cell Res. 303:331-342.
11. Hieshima, K., H. Ohtani, M. Shibano, D. Izawa, T. Nakayama, Y. Kawasaki, F. Shiba, M. Shiota, F. Katou, T. Saito, and O. Yoshie. 2003. CCL28 has dual roles in mucosal immunity as a chemokine with broad-spectrum antimicrobial activity. J. Immunol. 170:1452-1461.
12. Humphrey, W., A. Dalke, and K. Schulten. 1996. VMD: visual molecular dynamics. J. Mol. Graph. 14:33-38.
13. Johansson, J., G. H. Gudmundsson, M. E. Rottenberg, K. D. Berndt, and B. Agerberth. 1998. Conformation-dependent antibacterial activity of the naturally occurring human peptide LL-37. J. Biol. Chem. 273:3718-3724.
14. Krijgsveld, J., S. A. Zaat, J. Meeldijk, P. A. van Veelen, G. Fang, B. Poolman, E. Brandt, J. E. Ehlert, A. J. Kuijpers, G. H. Engbers, J. Feijen, and J. Dankert. 2000. Thrombocidins, microbicidal proteins from human blood platelets, are C-terminal deletion products of CXC chemokincs. J. Biol. Chem. 275:20374-20381.
15. + CXC chemokines in human milk. Cytokine 24:91-102.
16. Mine, S., K. Nasu, J. Fukuda, B. Sun, and I. Miyakawa. 2003. Secretion of granulocyte chemotactic protein-2 by cultured human endometrial stromal cells. Fertil. Steril. 79:146-150.
17. Prause, O., M. Laan, J. Lotvall, and A. Linden. 2003. Pharmacological modulation of interleukin-17-induced GCP-2-, GRO-α- and interleukin-8 release in human bronchial epithelial cells. Eur. J. Pharmacol. 462:193-198.
18. Proost, P., C. De Wolf-Peeters, R. Conings, G. Opdenakker, A. Billiau, and J. Van Damme. 1993. Identification of a novel granulocyte chemotactic protein (GCP-2) from human tumor cells. In vitro and in vivo comparison with natural forms of GRO, IP-10, and IL-8. J. Immunol. 150:1000-1010.
19. Proudfoot, A. E. 2006. The biological relevance of chemokine-proteoglycan interactions. Biochem. Soc. Trans. 34:422-426.
20. Rice, P., I. Longden, and A. Bleasby. 2000. EMBOSS: the European Molecular Biology Open Software Suite. Trends Genet. 16:276-277.
21. Roth, J., M. Bendayan, and L. Orci. 1978. Ultrastructural localization of intracellular antigens by the use of protein A-gold complex. J. Histochem. Cytochem. 26:1074-1081.
22. Sachse, F., F. Ahlers, W. Stoll, and C. Rudack. 2005. Neutrophil chemokines in epithelial inflammatory processes of human tonsils. Clin. Exp. Immunol. 140:293-300.
23. Schwede, T., J. Kopp, N. Guex, and M. C. Peitsch. 2003. SWISS-MODEL: an automated protein homology-modeling server. Nucleic Acids Res. 31:3381-3385.
24. Sumby, P., S. Zhang, A. R. Whitney, F. Falugi, G. Grandi, E. A. Graviss, F. R. Deleo, and J. M. Musser. 2008. A chemokine-degrading extracellular protease made by group A Streptococcus alters pathogenesis by enhancing evasion of the innate immune response. Infect. Immun. 76:978-985.
25. Territo, M. C., T. Ganz, M. E. Selsted, and R. Lehrer. 1989. Monocytechemotactic activity of defensins from human neutrophils. J. Clin. Investig. 84:2017-2020.
26. Wolf, M., M. B. Delgado, S. A. Jones, B. Dewald, I. Clark-Lewis, and M. Baggiolini. 1998. Granulocyte chemotactic protein 2 acts via both IL-8 receptors, CXCR1 and CXCR2. Eur. J. Immunol. 28:164-170.
27. Wuyts, A., P. Proost, J. P. Lenaerts, A. Ben-Baruch, J. Van Damme, and J. M. Wang. 1998. Differential usage of the CXC chemokine receptors 1 and 2 by interleukin-8, granulocyte chemotactic protein-2 and epithelial-cell-derived neutrophil attractant-78. Eur. J. Biochem. 255:67-73.
28. Wuyts, A., S. Struyf, K. Gijsbers, E. Schutyser, W. Put, R. Conings, J. P. Lenaerts, K. Geboes, G. Opdenakker, P. Menten, P. Proost, and J. Van Damme. 2003. The CXC chemokine GCP-2/CXCL6 is predominantly induced in mesenchymal cells by interleukin-1β and is down-regulated by interferon-gamma: comparison with interleukin-8/CXCL8. Lab. Investig. 83:23-34.
29. Yang, D., Q. Chen, D. M. Hoover, P. Staley, K. D. Tucker, J. Lubkowski, and J. J. Oppenheim. 2003. Many chemokines including CCL20/MIP-3α display antimicrobial activity. J. Leukoc. Biol. 74:448-455.