Vectors that facilitate the expression and purification of foreign peptides in Escherichia coli by fusion to maltose-binding protein

Gene

Volumn 67, Issue 1, 1988, Pages 21-30

  di Guana, Chu ^a   Lib, Ping ^b   Riggsa, Paul D ^a   Inouyeb, Hiroshi ^b  

^a NEW ENGLAND BIOLABS   (United States)

^b TEMPLE UNIVERSITY   (United States)

Author keywords

cross linked amylose affinity chromatography; plasmids; Recombinant DNA; starch

Indexed keywords

HYBRID PROTEIN; MALTOSE BINDING PROTEIN;

ESCHERICHIA COLI; GENE EXPRESSION; GENETIC ENGINEERING; HEREDITY; METHODOLOGY; NONHUMAN; PLASMID;

ALKALINE PHOSPHATASE; BASE SEQUENCE; CARRIER PROTEINS; CLONING, MOLECULAR; DNA RESTRICTION ENZYMES; ESCHERICHIA COLI; GENES, STRUCTURAL; GENETIC VECTORS; GENOTYPE; MEMBRANE PROTEINS; MOLECULAR SEQUENCE DATA; RECOMBINANT FUSION PROTEINS; RECOMBINANT PROTEINS; SUPPORT, NON-U.S. GOV'T;

EID: 0023680201     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/0378-1119(88)90004-2     Document Type: Article

References (19)

1. Analysis of the regulation of Escherichia coli alkaline phosphatase synthesis using deletions and φ80 transducing phages
2. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid
3. Sequences of the malE gene and of its product, the maltose-binding protein of Escherichia coli K-12
4. Affinity Chromatographie isolation of the periplasmic maltose-binding protein of Escherichia coli
5. Fusions of secreted proteins to alkaline phosphatase: an approach for studying protein secretion
6. Maltose binding protein from Escherichia coli
7. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
8. Production of specific antibodies against protein A fusion proteins
9. The release of ribonuclease into the medium when Escherichia coli cells are converted to spheroplasts
10. The release of enzymes from Escherichia coli by osmotic shock and during the formation of spheroplasts
11. DNA sequencing with chain terminating inhibitors
12. Identification of the malK gene product
13. Labeling of proteins with β-galactosidase by gene fusion
14. Use of aphoB'−'lacZ fusion gene to determine the N-terminal amino acid sequence of the PhoB protein and to prepare antiserum against the protein
15. The organization and complete nucleotide sequence of the PstI restrictionmodification system
16. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors