Humanization of antagonistic anti-human 4-1BB monoclonal antibody using a phage-displayed combinatorial library

Journal of Immunotherapy

Volumn 27, Issue 3, 2004, Pages 201-210

  Lee, Unn Hwa ^a   Son, Ji Hee ^a   Lee, Jeong Jin ^b   Kwon, Byungsuk ^a   Park, Jeong Woo ^a   Kwon, Byoung Se ^a  

^a University of Ulsan   (South Korea)

^b LG Chemical Ltd   (South Korea)

Author keywords

Antagonistic mAb; Human 4 1BB; Humanization; Phage display; scFv

Indexed keywords

CD137 ANTIGEN; DNA; HUMAN MONOCLONAL ANTIBODY; HUMAN MONOCLONAL ANTIBODY 4 1BB; MONOCLONAL ANTIBODY; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY; UNCLASSIFIED DRUG;

ARTICLE; CELL PROLIFERATION; DNA SEQUENCE; ENZYME LINKED IMMUNOSORBENT ASSAY; FLUORESCENCE ACTIVATED CELL SORTING; GENE EXPRESSION; GENE LIBRARY; HUMAN; HUMAN CELL; IMMUNOGLOBULIN VARIABLE REGION; MONONUCLEAR CELL; PHAGE DISPLAY; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL;

EID: 4344654055     PISSN: 15249557     EISSN: None     Source Type: Journal    
DOI: 10.1097/00002371-200405000-00004     Document Type: Article

References (35)

1. Pollok KE, Kim YJ, Zhou Z, et al. Inducible T cell antigen 4-1BB. Analysis of expression and function. J Immunol. 1993;150:771-781.
2. Melero I, Johnston JV, Shufford WW, et al. NK1.1 cells express 4-1BB (CDw137) costimulatory molecule and are required for tumor immunity elicited by anti-4-1BB monoclonal antibodies. Cell Immunol. 1998;190: 167-172.
3. Futagawa T, Akiba H, Kodama T, et al. Expression and function of 4-1BB and 4-1BB ligand on murine dendritic cells. Int Immunol. 2002;14:275-286.
4. Wilcox RA, Chapoval AI, Gorski KS, et al. Cutting edge: expression of functional CD 137 receptor by dendritic cells. J Immunol. 2002;168:4262-4267.
5. Kim YJ, Kim SH, Mantel P, et al. Human 4-1BB regulates CD28 co-stimulation to promote Th1 cell responses. Eur J Immunol. 1998;28:881-890.
6. Lee HW, Park SJ, Choi BK, et al. 4-1BB promotes the survival of CD8+ T lymphocytes by increasing expression of Bcl-xL and Bfl-1. J Immunol. 2002;169:4882-4888.
7. Lee UH, Kwack KB, Park JW, et al. Molecular cloning of agonistic and antagonistic monoclonal antibodies against human 4-1BB. Eur J Immunogenet. 2002;29:449-452.
8. Melero I, Shuford WW, Newby SA, et al. Monoclonal antibodies against the 4-1BB T-cell activation molecule eradicate established tumors. Nat Med. 1997;3:682-685.
9. Miller RE, Jones J, Le T, et al. 4-1BB-specific monoclonal antibody promotes the generation of tumor-specific immune responses by direct activation of CD8 T cells in a CD40-dependent manner. J Immunol. 2002; 169:1792-1800.
10. Ye Z, Hellstrom I, Hayden-Ledbetter M, et al. Gene therapy for cancer using single-chain Fv fragments specific for 4-1BB. Nat Med. 2002;8: 343-348.
11. Bertram EM, Lau P, Watts T. Temporal segregation of 4-1BB versus CD28-mediated costimulation: 4-1BB ligand influences T cell numbers late in the primary response and regulates the size of the T cell memory response following influenza infection. J Immunol. 2002;168:3777-3785.
12. Halstead ES, Mueller YM, Altman JD, et al. In vivo stimulation of CD137 broadens primary antiviral CD8+ T cell responses. Nat Immunol. 2002;3: 536-541.
13. Tan JT, Whitmire JK, Murali-Krishna K, et al. 4-1BB costimulation is required for protective anti-viral immunity after peptide vaccination. J Immunol. 2000;164:2320-2325.
14. Mittler RS, Bailey TS, Klussman K, et al. Anti-4-1BB monoclonal antibodies abrogate T cell-dependent humoral immune responses in vivo through the induction of helper T cell anergy. J Exp Med. 1999;190:1535-1540.
15. Foell J, Strahotin S, O'Neil SP, et al. CD137 costimulatory T cell receptor engagement reverses acute disease in lupus-prone NZB x NZW F1 mice. J Clin Invest. 2003;111:1505-1518.
16. Sun Y, Chen HM, Subudhi SK, et al. Costimulatory molecule-targeted antibody therapy of a spontaneous autoimmune disease. Nat Med. 2002; 8:1405-1413.
17. Foell J, McCausland M, Burch J, et al. CD137-mediated T cell costimulation terminates existing autoimmune disease in SLE-prone NZB/NZW F1 mice. Ann NY Acad Sci. 2003;987:230-235.
18. Sun Y, Lin X, Chen HM, et al. Administration of agonistic anti-4-1BB monoclonal antibody leads to the amelioration of experimental autoimmune encephalomyelitis. J Immunol. 2002;168:1457-1465.
19. Garni-Wagner BA, Lee ZH, Kim YJ, et al. 4-1BB is expressed on CD45RAhiROhi transitional T cell in humans. Cell Immunol. 1996;169: 91-98.
20. Son JH, Lee UH, Lee JJ, et al. Humanization of agonistic anti-human 4-1BB monoclonal antibody using a phage-displayed combinatorial library. J Immunol Meth. 2004; (in press)
21. Mian IS, Bradwell AR, Olson AJ. Structure, function and properties of antibody binding sites. J Mol Biol. 1991;217:133-151.
22. Chothia C, Lesk AM, Tramontano A, et al. Conformations of immunoglobulin hypervariable regions. Nature. 1989;342:877-883.
23. Foote J, Winter G. Antibody framework residues affecting the conformation of the hypervariable loops. J Mol Biol. 1992;224:487-499.
24. Kettleborough CA, Saldanha J, Heath VJ, et al. Humanization of a mouse monoclonal antibody by CDR-grafting: the importance of framework residues on loop conformation, Protein Eng. 1991;4:773-783.
25. Chothia C, Novotny J, Bruccoleri R, et al. Domain association in immunoglobulin molecules. The packing of variable domains. J Mol Biol. 1985; 186:651-663.
26. Rosok MJ, Yelton DE, Harris LJ, et al. A combinatorial library strategy for the rapid humanization of anticarcinoma BR96 Fab. J Biol Chem. 1996;271:22611-22618.
27. Kabat EA, Wu TT, Reid-Miller M, et al. Sequences of Proteins of Immunological Interest. U.S. Department of Health and Human Services, NIH, Bethesda, MD; 1987.
28. Padlan EA. A possible procedure for reducing the immunogenicity of antibody variable domains while preserving their ligand-binding properties. Mol Immunol. 1991;28:489-498.
29. Wang S, Kim YJ, Bick C, et al. The potential roles of 4-1BB costimulation in HIV type 1 infection. AIDS Res Hum Retroviruses. 1998;14:223-231.
30. Shuford WW, Klussman K, Tritchler DD, et al. 4-1BB costimulatory signals preferentially induce CD8+ T cell proliferation and lead to the amplification in vivo of cytotoxic T cell responses. J Exp Med. 1997;186: 47-55.
31. De Bruin R, Spelt K, Mol J, et al. Selection of high-affinity phage antibodies from phage display libraries. Nat Biotechnol. 1999;17:397-399.
32. Takahashi C, Mittler RS, Vella AT. Differential clonal expansion of CD4 and CD8 T cells in response to 4-1BB ligation: contribution of 4-1BB during inflammatory responses. Immunol Lett. 2001;76:183-191.
33. Michel J, Schwarz H. Expression of soluble CD137 correlates with activation-induced cell death of lymphocytes. Cytokine. 2000;12:742-746.
34. Li Q, Carr A, Ito F, et al. Polarization effects of 4-1BB during CD28 costimulation in generating tumor-reactive T cells for cancer immunotherapy. Cancer Res. 2003;63:2546-2552.
35. Glaser SM, Yelton DE, Huse WD. Antibody engineering by codon-based mutagenesis in a filamentous phage vector system. J Immunol. 1992;149: 3903-3913.