Biosynthesis, purification, and characterization of a cannabinoid receptor 2 fragment (CB2271-326)

Protein Expression and Purification

Volumn 59, Issue 2, 2008, Pages 249-257

  Zhang, Yuxun ^a   Xie, Xiang Qun ^a,b  

^a UNIVERSITY OF PITTSBURGH   (United States)

^b UNIVERSITY OF PITTSBURGH SCHOOL OF MEDICINE   (United States)

Author keywords

Affinity chromatography; Cannabinoid receptor 2; Circular dichroism; Fluorescence; HPLC; Inclusion body; Transmembrane domain

Indexed keywords

CANNABINOID 2 RECEPTOR; CETOMACROGOL; DETERGENT; DIGITONIN; HYBRID PROTEIN; NICKEL; PEPTIDE FRAGMENT; PROTEINASE; SIGNAL PEPTIDE; TEV PROTEASE;

AFFINITY CHROMATOGRAPHY; ARTICLE; BIOSYNTHESIS; CELL INCLUSION; CHEMISTRY; CIRCULAR DICHROISM; ESCHERICHIA COLI; GENETICS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN DENATURATION; PROTEIN FOLDING; PROTEIN MOTIF; PROTEIN SECONDARY STRUCTURE; PROTEIN TERTIARY STRUCTURE; SPECTROFLUOROMETRY;

AMINO ACID MOTIFS; CETOMACROGOL; CHROMATOGRAPHY, AFFINITY; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CIRCULAR DICHROISM; DETERGENTS; DIGITONIN; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENDOPEPTIDASES; ESCHERICHIA COLI; HUMANS; INCLUSION BODIES; NICKEL; PEPTIDE FRAGMENTS; PROTEIN DENATURATION; PROTEIN FOLDING; PROTEIN SORTING SIGNALS; PROTEIN STRUCTURE, SECONDARY; PROTEIN STRUCTURE, TERTIARY; RECEPTOR, CANNABINOID, CB2; RECOMBINANT FUSION PROTEINS; SPECTROMETRY, FLUORESCENCE;

ESCHERICHIA COLI; NICOTIANA TABACUM; TOBACCO ETCH VIRUS;

EID: 42749091750     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2008.02.005     Document Type: Article

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