Biochemical and mass spectrometric characterization of the human CB2 cannabinoid receptor expressed in Pichia pastoris-Importance of correct processing of the N-terminus

Protein Expression and Purification

Volumn 55, Issue 2, 2007, Pages 225-235

  Zhang, Rundong ^a   Kim, Tae Kang ^a   Qiao, Zhaun Hong ^a   Cai, Jian ^a   Pierce Jr , William M ^a   Song, Zhao Hui ^a  

^a University of Louisville School of Medicine   (United States)

Author keywords

Factor; Cannabinoid receptor; G protein coupled receptor; Mass spectrometry; Pichia Pastoris; Protein expression

Indexed keywords

CANNABINOID 2 RECEPTOR; PRIMER DNA; RECOMBINANT PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; CHEMISTRY; GENETICS; GLYCOSYLATION; HUMAN; METABOLISM; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PICHIA; POLYACRYLAMIDE GEL ELECTROPHORESIS; RADIOASSAY; TANDEM MASS SPECTROMETRY; WESTERN BLOTTING;

AMINO ACID SEQUENCE; BASE SEQUENCE; BLOTTING, WESTERN; DNA PRIMERS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GLYCOSYLATION; HUMANS; MOLECULAR SEQUENCE DATA; PICHIA; RADIOLIGAND ASSAY; RECEPTOR, CANNABINOID, CB2; RECOMBINANT PROTEINS; TANDEM MASS SPECTROMETRY;

PICHIA PASTORIS;

EID: 34548393181     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2007.03.018     Document Type: Article

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