Expression, purification, and activities of full-length and truncated versions of the integral membrane protein Vpu from HIV-1

Protein Science

Volumn 11, Issue 3, 2002, Pages 546-557

  Ma , Che ^a   Marassi, Francesca M ^b   Jones, David H ^a   Straus, Suzana K ^e   Bour, Stephan ^c   Strebel, Klaus ^c   Schubert, Ulrich ^c   Oblatt Montal, Myrta ^d   Montal, Mauricio ^d   Opella, Stanley J ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b BURNHAM INSTITUTE   (United States)

^c NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES   (United States)

^d NEUROBIOLOGY SECTION   (United States)

^e NONE

Author keywords

HIV 1; Ion channel activity; Membrane proteins; NMR spectroscopy; Overexpression; Vpu

Indexed keywords

CD4 ANTIGEN; HYBRID PROTEIN; ION CHANNEL; MEMBRANE PROTEIN; RECEPTOR PROTEIN; VPU PROTEIN;

ALPHA HELIX; AMINO TERMINAL SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; CYTOPLASM; HUMAN; HUMAN CELL; HUMAN IMMUNODEFICIENCY VIRUS 1; HUMAN IMMUNODEFICIENCY VIRUS INFECTION; LIPID BILAYER; NONHUMAN; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; OLIGOMERIZATION; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN PURIFICATION; PROTEIN STRUCTURE; VIRUS PARTICLE;

AMINO ACID SUBSTITUTION; ANTIGENS, CD4; CLONING, MOLECULAR; GENE PRODUCTS, VPU; HIV-1; ION CHANNELS; MAGNETIC RESONANCE SPECTROSCOPY; METHIONINE; PROTEIN FOLDING; PROTEIN STRUCTURE, SECONDARY; RADIOISOTOPES;

HUMAN IMMUNODEFICIENCY VIRUS; HUMAN IMMUNODEFICIENCY VIRUS 1; RNA VIRUSES;

EID: 0036181837     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1110/ps.37302     Document Type: Article

References (45)

1. Production of acquired immunodeficiency syndrome-associated retrovirus in human and nonhuman cells transfected with an infectious molecular clone
2. Are there dominant membrane protein families with a given number of helices?
3. High-performance liquid-chromatographic purification of extremely hydrophobic peptides-transmembrane segments
4. Cell surface CD4 inhibits HTV-1 particle release by interfering with Vpu activity
5. The human immunodeficiency virus type 1 Vpu protein specifically binds to the cytoplasmic domain of CD4: Implications for the mechanism of degradation
6. Human immunodeficiency virus (HIV) type 2 envelope protein is a functional complement to HIV type 1 Vpu that enhances particle release of heterologous retroviruses
7. Lack of effect of cytoplasmic tail truncations on human immunodeficiency virus type 2 ROD Env particle release activity
8. Identification of a protein encoded by the vpu gene of HIV-1
9. Prediction of transmembrane alpha-helices in procariotic membrane proteins: The Dense Alignment Surface method
10. A soluble form of CD4 (T4) protein inhibits AIDS virus infection
11. Transmembrane domains of viral ion channel proteins: A molecular dynamics simulation study
12. Ion channels formed by HIV-1 Vpu: A modelling and simulation study
13. Selective cleavage of methionyl peptide bonds in ribonuclease with cyanogen bromide
14. Correlating backbone amide and side chain resonances in larger proteins by multiple relayed triple resonance NMR
15. The human immunodeficiency virus type I-specific protein vpu is required for efficient virus maturation and release
16. Vpu transmembrane peptide structure obtained by site-specific Fourier transform infrared dichroism and global molecular dynamics searching
17. A simple method for displaying the hydropathic character of a protein
18. Human immunodeficiency virus type 1 Vpu protein is an oligomeric type I integral membrane protein
19. Correlation of the structural and functional domains in the membrane protein Vpu from HIV-1
20. Complete resolution of the solid-state NMR spectrum of a uniformly 15N-labeled membrane protein in phospholipid bilayers
21. A novel human WD protein, h-bTrCP, that interacts with HIV-1 Vpu connects CD4 to the ER degradation pathway through an F-box motif
22. HIV accessory proteins as therapeutic targets
23. Improved sensitivity of HSQC spectra of exchange protons at short interscan delays using a new fast HSQC (FHSQC) detection scheme that avoid water saturation
24. NMR and membrane proteins
25. Structures of the M2 channel-lining segments from nicotinic acetylcholine and NMDA receptors by NMR spectroscopy
26. The amino-terminal region of Vpr from human immunodeficiency virus type 1 forms ion channels and kills neurons
27. Complete nucleotide sequence of the AIDS virus, HTLV-III
28. The two biological activities of human immunodeficiency virus type 1 Vpu protein involve two separable structural domains
29. Identification of an ion channel activity of the Vpu transmembrane domain and its involvement in the regulation of virus release from HIV-1-infected cells
30. The human immunodeficiency virus type 1 encoded Vpu protein is phosphorylated by casein kinase-2 (CK-2) at positions Ser52 and Set56 within a predicted a-helix-turn-a-helix-motif
31. Formation of a native like subdomain in a partially folded intermediate of bovine pancreatic trypsin inhibitor
32. Structure and function of HIV-I Vpu
33. Molecular and biochemical analyses of human immunodeficiency virus type 1 vpu protein
34. A novel gene of HIV-1, vpu, and its 16-kilodalton product
35. Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned gene
36. Functional role of human immunodeficiency virus type 1 vpu
37. Mapping the energy surface of transmembrane helix-helix interactions
38. Sequences in the cytoplasmic domain of CD4 are necessary and sufficient to confer sensitivity to the human immunodeficiency virus type 1 Vpu protein
39. Human immunodeficiency virus type I Vpu protein induces rapid degradation of CD4
40. Human immunodeficiency virus type 1 Vpu protein regulates the formation of intracellular gpl60-CD4 complexes
41. 1H, 13C and 15N random coil NMR chemical shifts of the common amino acids
42. HNCACB, a high-sensitivity 3D NMR experiment to correlate amide protons and nitrogen resonances with the alpha- and beta-carbon resonances in proteins
43. A suite of triple resonance NMR experiments for the backbone assignments of 15N, 13C, 2H labeled proteins with high sensitivity
44. Structural study of the HIV-1 accessory protein Vpu via synchrotron X-ray reflectivity from langmuir monolayers