Cloning and heterologous production of hiracin JM79, a Sec-dependent bacteriocin produced by Enterococcus hirae DCH5, in lactic acid bacteria and Pichia pastoris

Applied and Environmental Microbiology

Volumn 74, Issue 8, 2008, Pages 2471-2479

  Sánchez, Jorge ^a   Borrero, Juan ^a   Gómez Sala, Beatriz ^a   Basanta, Antonio ^a   Herranz, Carmen ^a   Cintas, Luis M ^a   Hernández, Pablo E ^a  

^a UNIVERSIDAD COMPLUTENSE DE MADRID   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

ENTEROCOCCUS FAECIUM; ENTEROCOCCUS HIRAE; PICHIA PASTORIS;

GENE EXPRESSION; LACTIC ACID;

BACTERIA;

BACTERIOCIN; ENTEROCIN P; HIRACIN JM79; NISIN; NISIN A; UNCLASSIFIED DRUG;

BACTERIUM; CLONE; FUNGUS; GENE; IMMUNITY; PLASMID;

ANTIMICROBIAL ACTIVITY; ARTICLE; BACTERIAL GENE; ENTEROCOCCUS FAECALIS; ENTEROCOCCUS FAECIUM; ENTEROCOCCUS HIRAE; EXPRESSION VECTOR; HIRIJM79 GENE; HIRJM79 GENE; LACTIC ACID BACTERIUM; LACTOBACILLUS SAKEI; LACTOCOCCUS LACTIS; MOLECULAR CLONING; NONHUMAN; PICHIA PASTORIS; PLASMID; PROMOTER REGION; PROTEIN PROCESSING; PROTEIN SYNTHESIS; STRUCTURAL GENE;

ANTI-BACTERIAL AGENTS; BACTERIAL PROTEINS; BACTERIOCINS; CLONING, MOLECULAR; ENTEROCOCCUS; GENE EXPRESSION; GENETIC VECTORS; LACTOBACILLUS; NISIN; PICHIA; RECOMBINANT PROTEINS;

ENTEROCOCCUS FAECALIS; ENTEROCOCCUS FAECIUM; ENTEROCOCCUS HIRAE; LACTOBACILLUS SAKEI; LACTOCOCCUS LACTIS; PICHIA PASTORIS;

EID: 42349096099     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.02559-07     Document Type: Article

References (50)

1. Aukrust, T. W., M. B. Brurberg, and I. F. Nes. 1995. Transformation of lactobacilli by electroporation. Methods Mol. Biol. 47:201-208.
2. Beaulieu, L., D. C. Groleau, B. Míguez, J. F. Jetté, H. Aomari, and M. Subirade. 2005. Production of pediocin PA-1 in the methylotrophic yeast Pichia pastoris reveals unexpected inhibition of its biological activity due to the presence of collagen-like material. Protein Expr. Purif. 43:111-125.
3. Casaus, P., T. Nilsen, L. M. Cintas, I. F. Nes, P. E. Hernández, and H. Holo. 1997. Enterocin B, a new bacteriocin from Enterococcus faecium T136 which can act synergistically with enteriocin A. Microbiology 143:2287-2294.
4. Chopin, M. C., A. Chopin, A. Moillo-Bott, and P. Langella. 1984. Two plasmid-determined restriction and modification systems in Streptococcus lactis. Plasmid 11:260-263.
5. Cintas, L. M., P. Casaus, L. S. Håvarstein, P. E. Hernández, and I. F. Nes. 1997. Biochemical and genetic characterization of enterocin P, a novel sec-dependent bacteriocin from Enterococcus faecium P13 with a broad antimicrobial spectrum. Appl. Environ. Microbiol. 63:4321-4330.
6. Cintas, L. M., P. Casaus, M. F. Fernández, and P. E. Hernández. 1998. Comparative antimicrobial activity of pediocin PA-1, enterocin L50, nisin A, and lactocin S against spoilage and foodborne pathogenic bacteria. Food Microbiol. 15:289-298.
7. Cintas, L. M., P. Casaus, C. Herranz, L. S. Håvarstein, H. Holo, P. E. Hernández, and I. F. Nes. 2000. Biochemical and genetic evidence that Enterococcus faecium L50 produces enterocins L50A and L50B, the sec-dependent enterocin P, and a novel bacteriocin secreted without an N-terminal extension termed enterocin Q. J. Bacteriol. 182:6806-6814.
8. Cintas, L. M., P. Casaus, C. Herranz, I. F. Nes, and P. E. Hernández. 2001. Bacteriocins of lactic acid bacteria. Food Sci. Technol. Int. 7:281-305.
9. Cortes-Perez, N. G., I. Poquet, M. Oliveira, J. J. Gratadoux, S. M. Madsen, A. Miyoshi, G. Corthier, V. Azevedo, P. Langella, and L. G. Bermúdez-Humarán. 2006. Construction and characterization of a Lactococcus lactis strain deficient in intracellular ClpP and extracellular HtrA proteases. Microbiology 152:2611-2618.
10. Cotter, P. D., C. Hill, and R. P. Ross. 2005. Bacteriocins: developing innate immunity for food. Nat. Rev. Microbiol. 3:777-788.
11. Criado, R., D. B. Diep, Å. Aakra, J. Gutiérrez, I. F. Nes, P. E. Hernández, and L. M. Cintas. 2006. Complete sequence of the enterocin Q-encoding plasmid pCIZ2 from the multiple bacteriocin producer Enterococcus faecium L50 and genetic characterization of enterocin Q production and immunity. Appl. Environ. Microbiol. 72:6653-6666.
12. Daly, R., and M. T. W. Hearn. 2005. Expression of heterologous proteins in Picha pastoris: a useful experimental tool in protein engineering and production. J. Mol. Recognit. 18:119-138.
13. De Vos, W. M. 1987. Gene cloning and expression in lactic streptococci. FEMS Microbiol. Rev. 46:281-295.
14. Dieye, Y., A. J. W. Hoekman, F. Clier, V. Juillard, H. J. Boot, and J. C. Piard. 2003. Ability of Lactococcus lactis to export viral capsid antigens: a crucial step for development of live vaccines. Appl. Environ. Microbiol. 69:7281-7288.
15. Ennahar, S., D. Aoude-Werner, O. Sorokine, A. van Dorsselaer, F. Bringel, J. C. Hubert, and C. Hassemann. 1996. Production of pediocin AcH by Lactobacillus plantarum WHE 92 isolated from cheese. Appl. Environ. Microbiol. 62:4381-4387.
16. Franz, C. M. A. P., M. J. van Belkum, W. H. Holzapfel, H. Abriouel, and A. Gálvez. 2007. Diversity of enterococcal bacteriocins and their grouping in a new classification scheme. FEMS Microbiol. Rev. 31:293-310.
17. Freitas, D. A., S. Leclerc, A. Miyoshi, S. C. Oliveira, P. S. M. Sommer, L. Rodrigues, A. Correa, M. Gautier, P. Langella, V. A. Azevedo, and Y. Le Loir. 2005. Secretion of Streptomyces tendae antifungal protein 1 by Lactococcus lactis. Braz. J. Med. Biol. Res. 38:1585-1592.
18. Gasson, M. J. 1983. Plasmid complements of Streptococcus lactis NCDO 712 and other lactic streptococci after protoplast-induced curing. J. Bacteriol. 154:1-9.
19. Guinane, C. M., P. D. Cotter, C. Hill, and R. P. Ross. 2005. Microbial solutions to microbial problems: lactococcal bacteriocins for the control of undesirable biota in food. J. Appl. Microbiol. 98:1316-1325.
20. Gutiérrez, J., R. Criado, R. Citti, M. Martín, C. Herranz, M. F. Fernández, L. M. Cintas, and P. E. Hernández. 2004. Performance and applications of polyclonal anti-peptide antibodies with specificity for the enterococcal bacteriocin enterocin P. J. Agric. Food Chem. 52:2247-2255.
21. Gutiérrez, J., R. Criado, M. Martín, C. Herranz, L. M. Cintas, and P. E. Hernández. 2005. Production of enterocin P, an antilisterial pediocin-like bacteriocin from Enterococcus faecium P13, in Pichia pastoris. Antimicrob. Agents Chemother. 49:3004-3008.
22. Gutiérrez, J., R. Criado, R. Citti, M. Martín, C. Herranz, I. F. Nes, L. M. Cintas, and P. E. Hernández. 2005. Cloning, production and functional expression of enterocin P, a sec-dependent bacteriocin produced by Enterococcus faecium P13, in Escherichia coli. Int. J. Food Microbiol. 103:239-250.
23. Gutiérrez, J., R. Larsen, L. M. Cintas, J. Kok, and P. E. Hernández. 2006. High-level heterologous production and functional expression of the sec-dependent enterocin P from Enterococcus faecium P13 in Lactococcus lactis. Appl. Microbiol. Biotechnol. 72:41-51.
24. Hancock, R. E. W., and H.-G. Sahl. 2006. Antimicrobial and host-defense peptides as new anti-infective therapeutic strategies. Nat. Biotechnol. 24:1551-1557.
25. Håvarstein, L. S., D. B. Diep, and I. F. Nes. 1995. A family of bacteriocin ABC transporters carry out proteolytic processing of their substrates concomitant with export. Mol. Microbiol. 16:229-240.
26. Herranz, C., and A. J. M. Driessen. 2005. Sec-mediated secretion of bacteriocin enterocin P by Lactococcus lactis. Appl. Environ. Microbiol. 71:1959-1963.
27. Holo, H., and I. F. Nes. 1989. High-frequency transformation, by electroporation, of Lactococcus lactis subsp. cremoris grown with glycine in osmotically stabilized media. Appl. Environ. Microbiol. 55:3119-3123.
28. Horn, N., M. I. Martínez, J. M. Martínez, P. E. Hernández, M. J. Gasson, J. M. Rodríguez, and H. M. Dodd. 1998. Production of pediocin PA-1 by Lactococcus lactis using the lactococcin A secretory apparatus. Appl. Environ. Microbiol. 64:818-823.
29. Ilgen, C., J. Lin-Cereghino, and J. M. Cregg. 2005. Pichia pastoris, p. 143-162. In G. Gellisen (ed.), Production of recombinant proteins. Novel microbial and eukaryotic expression systems. Wiley-VCH Verlag Gmbh & Co., Weinheim, Germany.
30. Jacob, A. E., and S. J. Hobbs. 1974. Conjugal transfer of plasmid-borne multiple antibiotic resistance in Streptococcus faecalis var. zymogenes. J. Bacteriol. 117:360-372.
31. Kadokura, H., F. Hatzen, and J. Beckwith. 2003. Protein disulphide bond formation in prokaryotes. Annu. Rev. Biochem. 72:111-135.
32. Kok, J., M. B. Jos, M. van der Vossen, and G. Venema. 1984. Construction of plasmid cloning vectors for lactic streptococci which also replicate in Bacillus subtilis and Escherichia coli. Appl. Environ. Microbiol. 48:726-731.
33. Kouwen, T. R., A. van der Goot, R. Dorenbos, T. Winter, H. Antelmann, M. C. Plaisier, W. J. Quax, J. M. van Dijl, and J.-Y. F. Dubois. 2007. Thiol-disulphide oxidorreductase molecules in the low-GC Gram-positive bacteria. Mol. Microbiol. 64:984-999.
34. Kuipers, O. P., P. G. G. A. de Ruyter, M. Kleerebezem, and W. M. de Vos. 1998. Quorum sensing-controlled gene expression in lactic acid bacteria. J. Biotechnol. 64:15-21.
35. Kuipers, A., J. Wirenga, R. Rink, L. D. Kluskens, A. J. M. Driessen, O. P. Kuipers, and G. N. Moll. 2006. Sec-mediated transport of posttranslationally dehydrated peptides in Lactococcus lactis. Appl. Environ. Microbiol. 72:7626-7633.
36. Martín, M., J. Gutiérrez, R. Criado, C. Herranz, L. M. Cintas, and P. E. Hernández. 2006. Genes encoding bacteriocins and their expression, and potential virulence factors of enterococci isolated from wood pigeons (Columba palumbus). J. Food Prot. 69:520-531.
37. Martín, M., J. Gutiérrez, R. Criado, C. Herranz, L. M. Cintas, and P. E. Hernández. 2007. Cloning, production and expression of the bacteriocin enterocin A produced by Enterococcus faecium PLBC21 in Lactococcus lactis. Appl. Microbiol. Biotechnol. 76:667-675.
38. Martín, M., J. Gutiérrez, R. Criado, C. Herranz, L. M. Cintas, and P. E. Hernández. 2007. Chimeras of mature pediocin PA-1 fused to the signal peptide of enterocin P permits the cloning, production, and expression of pediocin PA-1 in Lactococcus lactis. J. Food Prot. 70:2792-2798.
39. Martínez, J. M., M. I. Martínez, A. Suárez, C. Herranz, P. Casaus, L. M. Cintas, J. M. Rodríguez, and P. E. Hernández. 1998. Generation of polyclonal antibodies of predetermined specificity against pediocin PA-1. Appl. Environ. Microbiol. 64:4536-4545.
40. Nes, I. F., D. B. Diep, and H. Holo. 2007. Bacteriocin diversity in Streptococcus and Enterococcus. J. Bacteriol. 189:1189-1198.
41. Sánchez, J., A. Basanta, B. Gómez-Sala, C. Herranz, L. M. Cintas, and P. E. Hernández. 2007. Antimicrobial and safety aspects, and biotechnological potential of bacteriocinogenic enterococci isolated from mallard ducks (Anas platyrhynchos). Int. J. Food Microbiol. 117:295-305.
42. Sánchez, J., D. B. Diep, C. Herranz, I. F. Nes, L. M. Cintas, and P. E. Hernández. 2007. Amino acid and nucleotide sequence, adjacent genes, and heterologous expression of hiracin JM79, a sec-dependent bacteriocin produced by Enterococcus hirae DCH5, isolated from Mallard ducks (Anas platyrhynchos). FEMS Microbiol. Lett. 270:227-236.
43. Schillinger, U., and F. K. Lucke. 1989. Antibacterial activity of Lactobacillus sake isolated from meat. Appl. Environ. Microbiol. 55:1901-1906.
44. Schoeman, H., M. A. Vivier, M. du Toit, L. M. T. Dicks, and I. S. Pretorius. 1999. The development of bactericidal yeast strains by expressing the Pediococcus acidilactici pediocin gene (pedA) in Saccharomyces cerevisiae. Yeast 15:647-656.
45. Suárez, A. M., J. M. Rodríguez, P. E. Hernández, and J. I. Azcona. 1996. Generation of polyclonal antibodies against nisin: immunization strategies and immunoassay development. Appl. Environ. Microbiol. 62:2117-2121.
46. Trotter, M., R. P. Ross, G. F. Fitzgerald, and A. Coffey. 2002. Lactococcus lactis DPC5598, a plasmid-free derivative of a commercial starter, provides a valuable alternative host for culture improvement studies. J. Appl. Microbiol. 93:134-143.
47. van de Guchte, M., J. M. van der Vossen, J. Kok, and G. Venema. 1989. Construction of a lactococcal expression vector: expression of hen egg white lysozyme in Lactococcus lactis subsp. lactis. Appl. Environ. Microbiol. 55:224-228.
48. Van Reenen, C. A., M. L. Chikindas, W. H. van Zyl, and L. M. T. Dicks. 2002. Characterization and heterologous expression of a class IIa bacteriocin, plantaricin 423 from Lactobacillus plantarum 423, in Saccharomyces cerevisiae. Int. J. Food Microbiol. 81:29-40.
49. van Wely, K. H. M., J. Swaving, R. Freudl, and A. J. M. Driessen. 2001. Translocation of proteins across the cell envelope of Gram-positive bacteria. FEMS Microbiol. Rev. 25:437-454.
50. Wu, J., S. Hu, and L. Cao. 2007. Therapeutic effect of nisin Z on subclinical mastitis in lactating cows. Antimicrob. Agents Chemother. 51:3131-3135.