Cloning, production and expression of the bacteriocin enterocin a produced by Enterococcus faecium PLBC21 in Lactococcus lactis

Applied Microbiology and Biotechnology

Volumn 76, Issue 3, 2007, Pages 667-675

  Martín, María ^a   Gutiérrez, Jorge ^a,b   Criado, Raquel ^a   Herranz, Carmen ^a   Cintas, Luis M ^a   Hernández, Pablo E ^a  

^a UNIVERSIDAD COMPLUTENSE DE MADRID   (Spain)

^b DUBLIN INSTITUTE OF TECHNOLOGY   (Ireland)

Author keywords

Bacteriocins; Enterocins A; Enterococcus faecium; Heterologous production; Lactococcus lactis; Sec dependent secretion

Indexed keywords

BACTERIOCINS; ENTEROCINS A; ENTEROCOCCUS FAECIUM; HETEROLOGOUS PRODUCTION; LACTOCOCCUS LACTIS; SEC-DEPENDENT SECRETION;

BACTERIA; BIOTECHNOLOGY; CLONING; GENE EXPRESSION;

AMINO ACIDS;

BACTERIOCIN; ENTEROCIN A; NISIN; NISIN A; SIGNAL PEPTIDE; UNCLASSIFIED DRUG;

ANTIMICROBIAL ACTIVITY; CLONE; GENE EXPRESSION; IMMUNITY; PEPTIDE; PLASMID;

ANTIMICROBIAL ACTIVITY; ARTICLE; BACTERIAL STRAIN; BACTERIUM TRANSFORMATION; BIOLOGICAL ACTIVITY; CHIMERA; CONTROLLED STUDY; ENTEROCOCCUS FAECIUM; EXPRESSION VECTOR; LACTOCOCCUS LACTIS; LISTERIA; MOLECULAR CLONING; NONHUMAN; RECOMBINANT PLASMID;

BACTERIOCINS; BASE SEQUENCE; BIOTECHNOLOGY; CLONING, MOLECULAR; DNA PRIMERS; DNA, BACTERIAL; ENTEROCOCCUS FAECIUM; GENE EXPRESSION; GENES, BACTERIAL; LACTOCOCCUS LACTIS; LISTERIA; MOLECULAR SEQUENCE DATA; NISIN; PLASMIDS; PROTEIN SORTING SIGNALS; RECOMBINANT FUSION PROTEINS; TRANSFORMATION, GENETIC;

ENTEROCOCCUS FAECIUM; LACTOCOCCUS LACTIS; LISTERIA;

EID: 34548033007     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-007-1044-3     Document Type: Article

References (34)

1. Beaulieu L, Groleau D, Míguez CB, Jetté JF, Aomari H, Subirade M (2005) Production of pediocin PA-1 in the methylotrophic yeast Pichia pastoris reveals unexpected inhibition of its biological activity due to the presence of collagen-like material. Protein Expr Purif 43:111-125
2. Biet F, Berjeaud JM, Worobo RW, Cenatempo Y, Fremaux C (1998) Heterologous expression of the bacteriocin mesenterocin Y105 using the dedicated transport system and the general secretory pathway. Microbiology 144:2845-2854
3. Casaus P, Nilsen T, Cintas LM, Nes IF, Hernández PE, Holo H (1997) Enterocin B, a new bacteriocin from Enterococcus faecium T136 which can act synergistically with enterocin A. Microbiology 143:2287-2294
4. Cintas LM (1995) Ph.D. thesis. Universidad Autónoma de Madrid, Spain
5. Cintas LM, Casaus P, Håvarstein LS, Hernández PE, Nes IF (1997) Biochemical and genetic characterization of EntP, a novel sec-dependent bacteriocin from Enterococcus faecium P13 with a broad antimicrobial spectrum. Appl Environ Microbiol 63:4321-4330
6. Cintas LM, Casaus P, Herranz C, Håvarstein LS, Holo H, Hernández PE, Nes IF (2000) Biochemical and genetic evidence that Enterococcus faecium L50 produces enterocins L50A and L50B, the sec-dependent enterocin P, and a novel bacteriocin secreted without an N-terminal extension termed enterocin Q. J Bacteriol 182:6806-6814
7. Cintas LM, Casaus P, Herranz C, Nes IF, Hernández PE (2001) Review: bacteriocins of lactic acid bacteria. Food Sci Technol Int 7:281-305
8. Cotter PD, Hill C, Ross RP (2005) Bacteriocins: developing innate immunity for food. Nat Rev Microbiol 3:777-788
9. Chopin MC, Chopin A, Moillo-Bott A, Langella P (1984) Two plasmid-determined restriction and modification systems in Streptococcus lactis. Plasmid 11:260-263
10. Ennahar S, Aoude-Werner D, Sorokine O, van Dorsselaer A, Bringel F, Hubert JC, Hassemann C (1996) Production of pediocin AcH by Lactobacillus plantarum WHE 92 isolated from cheese. Appl Environ Microbiol 62:4381-4387
11. Franz CMAP, Worobo RW, Quadri LEN, Schillinger U, Holzapfel WH, Vederas JC, Stiles ME (1999) Atypical genetic locus associated with constitutive production of enterocin B by Enterococcus faecium BFE 900. Appl Environ Microbiol 65:2170-2178
12. Franz CMAP, Stiles ME, Schleifer KH, Holzapfel WH (2003) Enterococci in foods - a conundrum for food safety. Int J Food Microbiol 88:105-122
13. Freitas DA, Leclerc S, Miyoshi A, Oliveira SC, Sommer PSM, Rodrigues L, Correa A, Gautier M, Langella P, Azevedo VA, Le Loir Y (2005) Secretion of Streptomyces tendae antifungal protein 1 by Lactococcus lactis. Braz J Med Biol Res 38:1585-1592
14. Gasson MJ (1983) Plasmid complements of Streptococcus lactis NCDO 712 and other lactic streptococci after protoplast-induced curing. J Bacteriol 154:1-9
15. Guinane CM, Cotter PD, Hill C, Ross RP (2005) Microbial solutions to microbial problems; lactococcal bacteriocins for the control of undesirable biota in food. J Appl Microbiol 98:1316-1325
16. Gutiérrez J, Criado R, Citti R, Martín M, Herranz C, Fernández MF, Cintas LM, Hernández PE (2004) Performance and applications of polyclonal anti-peptide antibodies with specificity for the enterococcal bacteriocin enterocin P. J Agric Food Chem 52:2247-2255
17. Gutiérrez J, Criado R, Citti R, Martín M, Herranz C, Nes IF, Cintas LM, Hernández PE (2005a) Cloning, production and functional expression of enterocin P, a sec-dependent bacteriocin produced by Enterococcus faecium P13, in Escherichia coli. Int J Food Microbiol 103:239-250
18. Gutiérrez J, Criado R, Martín M, Herranz C, Cintas LM, Hernández PE (2005b) Production of enterocin P, an antilisterial pediocin-like bacteriocin from Enterococcus faecium P13, in Pichia Pastoris. Antimicrob Agents Chemother 49:3004-3008
19. Gutiérrez J, Larsen R, Cintas LM, Kok J, Hernández PE (2006) High-level heterologous production and functional expression of the sec-dependent enterocin P from Enterococcus faecium P13 in Lactococcus lactis. Appl Microbiol Biotechnol 72:41-51
20. Herranz C, Driessen AJM (2005) Sec-mediated secretion of enterocin P by Lactococcus lactis. Appl Environ Microbiol 71:1959-1963
21. Holo H, Nes IF (1989) High-frequency transformation, by electroporation, of Lactococcus lactis subsp. cremoris grown with glycine in osmotically stabilized media. Appl Environ Microbiol 55:3119-3123
22. Horn N, Martínez MI, Martínez JM, Hernández PE, Gasson MJ, Rodríguez JM, Dodd HM (1998) Production of pediocin PA-1 by Lactococcus lactis using the lactococcin A secretory apparatus. Appl Environ Microbiol 64:818-823
23. Ingham AB, Sproat KW, Tizard MLV, Moore RJ (2005) A versatile system for the expression of nonmodified bacteriocins in Escherichia coli. J Appl Microbiol 98:676-683
24. Klocke M, Mundt K, Idler F, Jung S, Bachausen JE (2005) Heterologous expression of enterocin A, a bacteriocin from Enterococcus faecium, fused to a cellulose-binding domain in Escherichia coli results in a functional protein with inhibitory activity against Listeria. Appl Microbiol Biotechnol 67:532-538
25. Kuipers OP, de Ruyter PGGA, Kleerebezem M, de Vos WM (1998) Quorum sensing-controlled gene expression in lactic acid bacteria. J Biotechnol 64:15-21
26. Martín M, Gutiérrez J, Criado R, Herranz C, Cintas LM, Hernández PE (2006) Genes encoding bacteriocins and their expression, and potential virulence factors of enterococci isolated from wood pigeons (Columba palumbus). J Food Prot 69:520-531
27. Martínez JM, Kok J, Sanders JW, Hernández PE (2000) Heterologous co-production of enterocin A and pediocin PA-1 by Lactococcus lactis: detection by specific peptide-directed antibodies. Appl Environ Microbiol 66:3543-3549
28. Moon GS, Pyun YR, Park MS, Ji GE, Kim WJ (2005) Secretion of recombinant pediocin PA-1 by Bifidobacterium longum, using the signal sequence for bifidobacterial a-amylase. Appl Environ Microbiol 71:5630-5632
29. Nilsen T, Nes IF, Holo H (1998) An exported inducer regulates bacteriocin production in Enterococcus faecium CTC492. J Bacteriol 180:1848-1854
30. O'Keefee T, Hill C, Ross RP (1999) Characterization and heterologous expression of the genes encoding enterocin A production, immunity and regulation in Enterococcus faecium DPC1146. Appl Environ Microbiol 65:1506-1515
31. Rodríguez JM, Cintas LM, Casaus P, Horn N, Dodd HM, Hernández PE, Gasson MJ (1995) Isolation of nisin-producing Lactococcus lactis strain from dry fermented sausages. J Appl Bacteriol 78:109-115
32. Schoeman H, Vivier MA, du Toit M, Dicks LMT, Pretorius IS (1999) The development of bactericidal yeast strains expressing the Pediococcus acidilactici pediocin gene (pedA) in Saccharomyces cerevisae. Yeast 15:647-656
33. Trotter M, Ross RP, Fitzgerald GF, Coffey A (2002) Lactococcus lactis DPC5598, a plasmid-free derivative of a commercial starter, provides a valuable alternative host for culture improvement studies. J Appl Microbiol 93:134-143
34. Van de Guchte M, van der Vossen JMBM, Kok J, Venema G (1989) Construction of a lactococcal expression vector: expression of hen egg white lysozyme in Lactococcus lactis subsp. lactis. Appl Environ Microbiol 55:224-228