γH2AX foci form preferentially in euchromatin after ionising-radiation

PLoS ONE

Volumn 2, Issue 10, 2007, Pages

  Cowell, Ian G ^a   Sunter, Nicola J ^a   Singh, Prim B ^b   Austin, Caroline A ^a   Durkacz, Barbara W ^a   Tilby, Michael J ^a  

^a NEWCASTLE UNIVERSITY   (United Kingdom)

^b RESEARCH CENTER BORSTEL   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; GAMMA HISTONE H2AX; HISTONE H2AX; HYDROXYUREA; UNCLASSIFIED DRUG; H2AFX PROTEIN, HUMAN; HISTONE;

ARTICLE; CELL CYCLE S PHASE; CELL STRAIN MCF 7; CONTROLLED STUDY; DNA DAMAGE; DNA REPAIR; DNA REPLICATION; EUCHROMATIN; GENOME; HETEROCHROMATIN; HUMAN; HUMAN CELL; IMMUNOFLUORESCENCE MICROSCOPY; IONIZING RADIATION; PROTEIN PHOSPHORYLATION; ULTRAVIOLET RADIATION; BIOLOGICAL MODEL; CELL NUCLEUS; CHEMISTRY; CHROMATIN; FLUORESCENCE MICROSCOPY; METABOLISM; NUCLEOLUS; PHOSPHORYLATION; PROTEIN TERTIARY STRUCTURE; TUMOR CELL LINE;

MAMMALIA;

CELL LINE, TUMOR; CELL NUCLEOLUS; CELL NUCLEUS; CHROMATIN; DNA DAMAGE; DNA REPLICATION; EUCHROMATIN; HETEROCHROMATIN; HISTONES; HUMANS; MICROSCOPY, FLUORESCENCE; MODELS, BIOLOGICAL; PHOSPHORYLATION; PROTEIN STRUCTURE, TERTIARY; RADIATION, IONIZING;

EID: 42249102663     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0001057     Document Type: Article

References (39)

1. Rogakou EP, Pilch DR, Orr AH, Ivanova VS, Bonner WM (1998) DNA double-stranded breaks induce historic H2AX phosphorylotion on scrine 139. J Biol Chem 273: 5858-5868.
2. Redon C, Pilch D, Rogakou E, Sedelnikova O, Newrock K, et al. (2002) Histone H2A variants H2AX and H2AZ. Curr Opin Genet Dev 12: 162-169.
3. Shiloh Y (2003) ATM and related protein kinases: safeguarding genome integrity. Nat Rev Cancer 3: 155-168.
4. Shiloh Y (2006) The ATM-mediated DNA-damage response; taking shape. Trends Biochem Sci 31: 402-410.
5. Stiff T, O'Driscoll M, Rief N, Iwabuchi K, Lobrich M, et al. (2004) ATM and DNA-PK function redundantly to phosphorylate H2AX after exposure to ionizing radiation. Cancer Res 64: 2390-2396.
6. Wang H, Wang M, Wang H, Bocker W, Iliakis G (2005) Complex H2AX phosphorylation patterns by multiple kinases including ATM and DNA-PK in human cells exposed to ionizing radiation and treated with kinase inhibitors. J Cell Physiol 202: 492-502.
7. Ward IM, Chen J (2001) Histone H2AX is phosphorylated in an ATR-dependent manner in response to replicational stress. J Biol Chem 276: 47759-47762.
8. Furuta T, Takemura H, Liao ZY, Aune GJ, Redon C, et al. (2003) Phosphorylation of histone H2AX and activation of Mre11, Rad50, and Nbs1 in response to replication-dependent DNA-double strand breaks induced by mammalian DNA topoisomerase I cleavage complexes. J Biol Chem. 278: 20303-20312.
9. Celeste A, Petersen S, Romanienko PJ, Fernandez-Capetillo O, Chen HT, et al. (2002) Genomic instability in mice lacking histone H2AX. Science 296: 922-927.
10. Bassing CH, Chua KF, Sekiguchi J, Suh H, Whitlow SR, et al. (2002) Increased ionizing radiation sensitivity and genomic instability in the absence of histone H2AX. Proc Natl Acad Sci U S A 99: 8173-8178.
11. Fernandez-Capetillo O, Chen HT, Celeste A, Ward I, Romanienko PJ, et al. (2002) DNA damage-induced G2-M checkpoint activation by historic H2AX and 53BP1. Nat Cell Biol 4: 993-997.
12. Bassing CH, Alt FW (2004) H2AX may function as an anchor to hold broken chromosomal DNA ends in dose proximity. Cell cycle 3: 149-153.
13. Cowell IG, Aucott R, Mahadevaiah SK, Burgoyne PS, Huskisson N, et al. (2002) Heterochromatin, HPI and methylation at lysine 9 of histone H3 in animals. Chromosoma 111: 22-36.
14. Wu R, Terry AV, Singh PB, Gilbert DM (2005) Differential Subnuclear Localization and Replication Timing of Historic H3 Lysine 9 Methylation States. Mol Biol Cell 16: 2872-2881.
15. Kourmouli N, Jeppesen P, Mahadevhaiah S, Burgoyne P, Wu R, et al. (2004) Heterochromatin and tri-methylated lysine 20 of histone H4 in animals. J Cell Sci 117: 2491-2501.
16. Cheutin T, McNairn AJ, Jenuwein T, Gilbert DM, Singh PB, et al. (2003) Maintenance of stable heterochromatin domains by dynamic HP1 binding. Science 299: 721-725.
17. Singh PB, Georgatos SD (2002) HP1: Facts, open questions, and speculation. J Struct Biol 140: 10-16.
18. Wreggett KA, Hill F, James PS, Hutchings A, Butcher GW, et al. (1994) A mammalian homologue of Drosophila heterochromatin protein 1 (HP1) is a component of constitutive heterochromatin. Cytogenet Cell Genet 66: 99-103.
19. Saunders WS, Chue C, Goebl M, Craig C, Clark RF, et al. (1993) Molecular cloning of a human homologue of Drosophils, heterochromatin protein HP1 using anti-centromere autoantibodies with anti-chromo specificity. J Cell Sci 104: 573-582.
20. James TC, Eissenberg JC, Craig G, Dietrich V, Hobson A, et al. (1989) Distribution patterns of HP1, a heterochromatin-associated nonhistone chromosomal protein of Drosophila. Eur J Cell Biol 50: 170-180.
21. Puerto S, Ramirez MJ, Marcos R, Creus A, Surralles J (2001) Radiation-induced chromosome aberrations in human euchromatic (17cen-p53) and heterochromatic (Icen-Iq12) regions. Mutagenesis 16: 291-296.
22. Mine E, Allory Y, Worman HJ, Courvalin JC, Buendia B (1999) Localization and phosphorylation of HP1 proteins during the cell cycle in mammalian cells. Chromosoma 108: 220-234.
23. Cowell IG, Durkacz BW, Tilby MJ (2005) Sensitization of breast carcinoma cells to ionizing radiation by small molecule inhibitors of DNA-dependent protein kinase and ataxia telangiectsia mutated. Biochem Pharmacol 71: 13-20.
24. Rogakou EP, Boon C, Redon C, Bonner WM (1999) Megabase chromatin domains involved in DNA double-strand breaks in vivo. J Cell Biol 146: 905-916.
25. Ward IM, Minn K, Chen J (2004) UV-induced ATR activation requires replication stress. J Biol Chem 279: 9677-9680.
26. Ten Hagen KG, Gilbert DM, Willard HF, Cohen SN (1990) Replication timing of DNA sequences associated with human centromeres and telomeres. Mol Cell Biol 10: 6348-6355.
27. Dimitrova DS, Gilbert DM (1999) The spatial position and replication timing of chromosomal domains are both established in early G1 phase. Mol Cell 4: 983-993.
28. Scully R, Chen J, Plug A, Xiao Y, Weaver D, et al. (1997) Association of the BRCA1 gcnc product with Rad51 in meiotic and mitotic cells. Cell 88: 265-275.
29. Fernandez-Capetillo O, Allis CD, Nussenzweig A (2004) Phosphorylation of Histone H2B at DNA Double-Strand Breaks. J Exp Med 199: 1671-1677.
30. Karagiannis TC, Kn H, El-Osta A (2007) Disparity of historic deacetylase inhibition on repair of radiation-induced DNA damage on euchromatin and constitutive heterochromatin compartments. Oncogene.
31. Kim JA, Kruhlak M, Dotiwala F, Nussenzweig A, Haber JE (2007) Heterochromatin is refractery to γ-H2AX modification in yeast and mammals. J Cell Biol 173: 209-218.
32. Brenner DJ, Ward JF (1992) Constraints an energy deposition and target size of multiply damaged sites associated with DNA double-strand breaks. Int J Radiat Biol 61: 737-748.
33. Ward JF (1985) Biochemistry of DNA lesions. Radiat Res Suppl 8; S103-111.
34. Ward JF (1994) The complexity of DNA damage: relevance to biological consequences. Int J Radiat Biol 66: 427-432.
35. Warters RL, Lyons BW (1992) Variation in radiation-induced formation of DNA double-strand breaks as a function of chromatin structure. Radiat Res 130: 309-318.
36. Martinez-Lopez W, Folle GA, Cassina G, Mendez-Acuna L, Di-Tomaso, MV, et al. (2004) Distribution of breakpoints induced by etoposide and X-rays along the CHO X chromosome. Cytogenet Genome Res 104: 182-187.
37. Taddei A, Maison C, Roche D, Almouzni G (2001) Reversible disruption of pericentric heterochromatin and centromere function by inhibiting deacetylases. Nat Cell Biol 3: 114-120.
38. Kruhlak MJ, Celeste A, Dellaire G, Fernandez-Capetillo O, Muller WG, et al. (2006) Changes in chromatin structure and mobility in living Cells at sites of DNA double-strand breaks. J Cell Biol 172: 823-834.
39. Kourmouli N, Theodoropoulos PA, Dialynas G, Bakou A, Politou AS, et al. (2000) Dynamic associations of hetcrochromatin protein 1 with the nuclear envelope. EMBO J 19: 6558-6568.