Evaluation of the in vitro micronucleus test as an alternative to the in vitro chromosomal aberration assay: Position of the GUM working group on the in vitro micronucleus test

Mutation Research - Reviews in Mutation Research

Volumn 410, Issue 1, 1998, Pages 81-116

  Miller, Beate ^a   Pötter Locher, Franziska ^b   Seelbach, Angelika ^c   Stopper, Helga ^d   Utesch, Dietmar ^e   Madle, Stephan ^c  

^a F HOFFMANN LA ROCHE LTD   (Switzerland)

^b NOVARTIS PHARMA AG   (Switzerland)

^c Fed Inst Hlth Protect C   (Germany)

^d UNIVERSITY OF WÜRZBURG   (Germany)

^e MERCK KGAA   (Germany)

Author keywords

Genotoxicity testing; GUM; In vitro chromosomal aberration test (CA); In vitro micronucleus test (MNT)

Indexed keywords

2 AMINOANTHRACENE; 2 NITROFLUORENE; 3 METHYLCHOLANTHRENE; AFLATOXIN B1; AMSACRINE; BENZO[A]PYRENE; BLEOMYCIN SULFATE; CADMIUM CHLORIDE; CARBENDAZIM; CHLORAL HYDRATE; COLCHICINE; CYCLOPHOSPHAMIDE; CYTOCHALASIN B; DACTINOMYCIN; DIAZEPAM; DIETHYLSTILBESTROL; DIMETHYLBENZ[A]ANTHRACENE; DOXORUBICIN; ECONAZOLE; FLUOROURACIL; GRISEOFULVIN; HYDROQUINONE; MESYLIC ACID ETHYL ESTER; METHYLNITRONITROSOGUANIDINE; METHYLNITROSOUREA; MITOMYCIN C; N (2 FLUORENYL)ACETAMIDE; PHENOL; PYRENE; PYRIMETHAMINE; THIOMERSAL; TIABENDAZOLE; VINCRISTINE SULFATE; ZINOSTATIN;

CHROMOSOME ABERRATION; DATA BASE; DRUG CYTOTOXICITY; GENOTOXICITY; HUMAN; HUMAN CELL; HUMAN TISSUE; INTERMETHOD COMPARISON; MICRONUCLEUS TEST; MUTAGEN TESTING; PRIORITY JOURNAL; REVIEW; TOXICITY TESTING;

EID: 0032006320     PISSN: 13835742     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1383-5742(97)00030-6     Document Type: Article

References (73)

1. Abe, S., Sasaki, M., 1977. Studies on chromosomal aberrations and sister chromatid exchanges induced by chemicals. Proc. Jpn. Acad. 53, 46-49.
2. Anderson, R.D., Berger, N.A., 1994. Mutagenicity and carcinogenicity of topoisomerase-interactive agents. Mutation Res. 309, 109-142.
3. Andersson, L.C., Lehto, V.P., Stenman, S., Bradley, R.A., Virtanen, I., 1981. Diazepam induces mitotic arrest at prometaphase by inhibiting centriolar separation. Nature 291, 247-248.
4. Antoccia, A., Tanzarella, C., Modesti, D., Degrassi, F., 1993. Cytokinesis-block micronucleus assay with kinetochore detection in colchicine-treated human fibroblasts. Mutation Res. 287, 93-99.
5. RMouse lac Z-mutation data for DMBA and its K-region sulphur isostere: comparison with activities observed in standard genotoxicity assays. Mutation Res. 292, 25-40.
6. Banduhn, N., Obe, G., 1985. Mutagenicity of methyl 2-benzimidazolecarbamate, diethylstilbestrol and estradiol: structural chromosomal aberrations, sister-chromatid exchanges, C-mitoses, polyploidies and micronuclei. Mutation Res. 156, 199-218.
7. Brinkley, B.R., Stubblefield, E., Hsu, T.C., 1967. The effects of colcemide inhibition and reversal on the fine structure of the mitotic apparatus of Chinese hamster cells in vitro. J. Ultrastruct. Res. 19, 1-18.
8. Cole, J., Diot, M.-C., Richmond, F.N., Bridges, B.A., 1990. Comparative induction of gene mutations and chromosome damage by 1-methoxy-1,3,5-cycloheptatriene (MCHT), 2. Results using L5178Y mouse lymphoma cells to detect both gene and chromosome damage; validation with ionizing radiation, ethyl methanesulphonate and benzo(a)pyrene. Mutation Res. 230, 81-91.
9. Darroudi, F., Natarajan, A.T., 1993. Metabolic activation of chemicals to mutagenic carcinogens by human hepatoma microsomal extracts in Chinese hamster ovary cells (in vitro). Mutagenesis 8, 11-15.
10. DeCarli, L., Larizza, L., 1988. Griseofulvin. Mutation Res. 195, 91-126.
11. Doerr, C.L., Harrington-Brock, K., Moore, M.M., 1989. Micronucleus, chromosome aberration, and small-colony TK mutant analysis to quantitate chromosomal damage in L5178Y mouse lymphoma cells. Mutation Res. 222, 191-203.
12. ECETOC (1997) Aneuploidy: its significance in human disease and methods for screening chemicals. ECETOC, Technical Report No. XX, Mutation Res., in press.
13. Eckert, I., Stopper, H., 1996. Disturbance of mitosis by β-estradiol in vitro. Toxicol. Vitro 10, 637-642.
14. Egeli, U., Erdogan, G., 1991. The clastogenic effect of pyrimethamine (Daraprim) on human chromosomes in lymphocyte cultures. Cell Biol. Toxicol. 7, 347-356.
15. Elhajouji, A., Santos, A.P., Van Hummelen, P., Kirsch-Volders, M., 1994. Metabolic differences between whole blood and isolated lymphocyte cultures for micronucleus (MN) induction by cyclophosphamide and benzo(a)pyrene. Mutagenesis 9, 307-313.
16. Ellard, S., Mohammed, Y., Dogra, S., Wölfel, C., Doehmer, J., Parry, J.M., 1991. The use of genetically engineered V79 Chinese hamster cultures expressing rat liver CYP1A1, 1A2 and 2B1 cDNAs in micronucleus assays. Mutagenesis 6, 461-470.
17. Fritzenschaf, H., Kohlpoth, M., Rusche, B., Schiffmann, D., 1993. Testing of known carcinogens and noncarcinogens in the Syrian Hamster Embryo (SHE) micronucleus test in vitro; correlation with in vivo micronucleus formation and cell transformation. Mutation Res. 319, 47-53.
18. Furnus, C.C., Ulrich, M.A., Terreras, M.C., Dulout, F.N., 1990. The induction of aneuploidy in cultured Chinese hamster cells by propionaldehyde and chloral hydrate. Mutagenesis 5, 323-326.
19. Galloway, S.M., Armstrong, M.J., Reuben, C., Colman, B., Brown, C., Cannon, A.D., Bloom, F., Nakamura, M., Ahmed, S., Duk, J., Rimpo, B.M., Margolin, M.A., Resnick, Anderson, B., 1987. Chromosome aberrations and sister chromatid exchanges in Chinese hamster ovary cells: evaluations of 108 chemicals. Environ. Mol. Mutagen. 10 (Suppl. 10), 1-175.
20. Glatt, H.R., Padykula, R., Berchtold, G.A., Ludewig, G., Platt, K.L., Klein, J., Oesch, F., 1989. Multiple activation pathways of Benzene leading to products with varying genotoxic characteristics. Environ. Health Perspect. 82, 81-89.
21. Glatt, H., Gemperlein, I., Setiabudi, F., Platt, K.L., Oesch, F., 1990. Expression of xenobiotic-metabolizing enzymes in propagatable cell cultures and induction of micronuclei by 13 compounds. Mutagenesis 5 (Suppl. 3), 241-249.
22. Goldberg, I.H., 1991. Mechanism of neocarzinostatin action: role of DNA microstructure in determination of chemistry of bistranded oxidative damage. Ace. Chem. Res. 24, 191-198.
23. Gu, Z.W., Whong, W.Z., Wallace, W.E., Ong, T.M., 1992. Induction of micronuclei in BALB/c-3T3 cells by selected chemicals and complex mixtures. Mutation Res. 279, 217-222.
24. Hsie, A.W., San Sebastian, J.R., Perdue, St.W., Schenley, R.L., Waters, M.D., 1987. Multi-endpoint mutagenesis with Chinese hamster ovary (CHO) cells: evaluation with eight carcinogenic and non-carcinogenic compounds. Mol. Toxicol. 1, 217-234.
25. Ivett, J.L., Brown, B.M., Rodgers, C., Anderson, B.E., Resnick, M.A., Zeiger, E., 1989. Chromosomal aberrations and sister chromatid exchange tests in Chinese hamster ovary cells in vitro IV. Results with 15 chemicals. Environ. Mol. Mutagen. 14, 165-187.
26. M. Ishidate, Data Book of Chromosomal Aberration Test In Vitro (revised edn.), Elsevier, Amsterdam, 1988.
27. Iskandar, O., Vijayalaxmi, 1981. The enhancement of the effect of aflatoxin B1 by metabolic activation with rat-liver microsomes on HULY assayed with the micronucleus test. Mutation Res. 91, 63-66.
28. 6-methylguanine-DNA methyltransferase (MGMT) cDNA in Chinese hamster ovary cells: the role of MGMT in protection against the genotoxic effects of alkylating agents. Carcinogenesis 12, 1857-1867.
29. S. Kalweit, S. Ertelt, B. Fissler, A. Seelbach, S. Madie, The micronucleus test in vitro with V79 cells: comparison of the cytokinetic block (CYTO B) assay and the mitotic shake off method (MSO), Eur. J. Genet. Toxicol., in press.
30. Kirkland, D.J., 1992. Chromosomal aberration test in vitro: problems with Protocol design and interpretation of results. Mutagenesis 7, 95-106.
31. Kirsch-Volders, M., Talion, I., Tanzarella, C., Sgura, A., Hermine, T., Parry, E.M., Parry, J.M., 1996. Mitotic non-disjunction as a mechanism for in vitro aneuploidy induction by X-rays in primary human cells. Mutagenesis 11, 307-313.
32. Krishan, A., Dutt, K., Israel, M., Ganapathi, R., 1981. Comparative effects of Adriamycin and N-Trifluoroacetyladriamycin-14-valerate on cell kinetics, chromosomal damage and macromolecular synthesis in vitro. Cancer Res. 41, 2745-2750.
33. Krishna, G., Nath, J., Ong, T., 1986. Inhibition of cyclophosphamide and mitomycin C induced sister chromatid exchanges in mice by vitamin C. Cancer Res. 46, 2670-2674.
34. Li, J., Suzuki, Y., Shimizu, H., Fukumoto, M., Okonogi, H., Nagashima, T., Ishikawa, T., 1993. In vitro micronucleus assay of 30 chemicals in CHL cells. Jikeikai Med. J. 40, 69-83.
35. Lindholm, C., Norppa, H., Hayashi, M., Sorsa, M., 1991. Induction of micronuclei and anaphase aberrations by cytochalasin B in human lymphocyte cultures. Mutation Res. 260, 369-375.
36. Maier, P., Schmid, W., 1976. Ten model mutagens evaluated by the micronucleus test. Mutation Res. 40, 325-338.
37. Majone, F., Tonetto, S., Soligo, C., Panozzo, M., 1992. Identification of kinetochores and DNA synthesis in micronuclei induced by mitomycin C and colchicine in Chinese hamster ovary cells. Teratog. Carcinog. Mutag. 12, 155-166.
38. Marcon, F., A. Zijno, P. Leopardi, R. Crebelli, Analysis of chromosome malsegregation in HULY after vinblastine treatment: synergistic effect of cytochalasin B on induced malsegregation rates, Eur. J. Genet. Toxicol., in press.
39. Marrazzini, A., Betti, C., Bernacchi, F., Barrai, I., Barale, R., 1994. Micronucleus test and metaphase analyses in mice exposed to known and suspect spindle poisons. Mutagenesis 9, 505-515.
40. Matsuoka, A., Sofuni, T., Miyata, N., Ishidate, M., 1991. Clastogenicity of 1-nitropyrene, dinitropyrenes, fluorene and mononitrofluorenes in cultured Chinese hamster cells. Mutation Res. 259, 103-110.
41. Matsuoka, A., Yamazaki, N., Suzuki, T., Hayashi, M., Sofuni, T., 1993. Evaluation of the micronucleus test using a Chinese hamster cell line as an alternative to the conventional in vitro chromosomal aberration test. Mutation Res. 272, 223-236.
42. Miller, B.M., Pujadas, E., Gocke, E., 1995. Evaluation of the micronucleus test in vitro using Chinese hamster cells: results of four chemicals weakly positive in the in vivo micronucleus test. Environ. Mol. Mutagen. 26, 240-247.
43. Migliore, L., Nieri, M., 1991. Evaluation of twelve potential aneuploidogenic chemicals by the in vitro human lymphocyte micronucleus assay. Toxicol. Vitro 5, 325-336.
44. Miller, B., S. Albertini, F. Locher, V. Thybaud, E. Lorge, Comparative evaluation of the in vitro micronucleus test and the in vitro chromosome aberration test: Industrial experience, Mutation Res., in press.
45. Namba, M., Kimoto, T., 1976. Cytotoxic effects of griseofulvin on human normal cells in vitro. Kawasaki Med. J. 2, 127-134.
46. Natarajan, A.T., Van Kesteren-van Leeuwen, A.C., 1981. Mutagenic activity of 20 coded compounds in chromosome aberrations/sister chromatid exchanges using Chinese hamster ovary (CHO) cells. Prog. Mutation Res. 1, 551-559.
47. Natarajan, A.T., Darroudi, F., 1991. Use of human hepatoma cells for in vitro metabolic activation of chemical mutagens/carcinogens. Mutagenesis 6, 399-403.
48. Norppa, H., Renzi, L., Lindholm, C., 1993. Detection of whole chromosomes in micronuclei of cytokinesis blocked HULY by antikinetochore staining and in situ hybridization. Mutagenesis 8, 519-525.
49. Ono, T., Norimatsu, M., Yoshimura, H., 1994. Induction of chromosome aberrations by pyrimethamine in cultured Chinese hamster (CHL) cells. Mutation Res. 323, 197-201.
50. Ono, T., Yoshimura, H., 1996. Analysis of micronucleus induction of pyrimethamine in in vitro CHL cells and in in vivo mouse bone marrow cells. Mutagenesis 11, 85-88.
51. Oshiro, Y., Piper, C.E., Balwierz, P.S., Soelter, S.G., 1991. Chinese hamster ovary cell assays for mutation and chromosome damage: data from noncarcinogens. J. Appl. Toxicol. 11, 167-177.
52. Parry, J.M., Sors, A., 1993. The detection and assessment of the aneugenic potential of environmental chemicals: the European Community Aneuploidy Project. Mutation Res. 287, 3-15.
53. M.J. Ramirez, S. Puerto, E. Carbonell, A. Creus, R. Marcos, Aneugenic activity of colchicine in HULY evaluated by means of the micronucleus assay and fish techniques, Eur. J. Genet. Toxicol., in press.
54. Russo, A., Pacchierotti, F., Metalli, P., 1984. Nondisjunction induced in mouse spermatogenesis by chloral hydrate and metabolite of trichlorethylene. Environ. Mol. Mutagen. 6, 695-703.
55. Salassidis et al. (1990).
56. Salvadori, D.M., 1994. Effect of β-carotene on clastogenic effects of mitomycin C, methyl methanesulphonate and bleomycin in Chinese Hamster ovary cells. Mutagenesis 9, 53-57.
57. Schmuck, G., Lieb, G., Wild, D., Schiffmann, D., Henschler, D., 1988. Characterization of an in vitro micronucleus assay with Syrian hamster embryo fibroblasts. Mutation Res. 203, 397-404.
58. Schnitzler, R., Foth, J., Degen, G.H., Metzler, M., 1994. Induction of micronuclei by stilbene-type and steroidal estrogens in Syrian hamster embryo and ovine seminal vesicle cells in vitro. Mutation Res. 311, 85-93.
59. Seelbach, A., Fissler, B., Strohbusch, A., Madle, S., 1993a. Development of a modified micronucleus assay in vitro for detection of aneugenic effects. Toxicol. Vitro 7, 185-193.
60. Seelbach, A., Fissler, B., Madle, S., 1993b. Further evaluation of a modified micronucleus assay with V79 cells for detection of aneugenic effects. Mutation Res. 303, 163-169.
61. Shelby, M.D., Witt, K.L., 1995. Comparison of results from mouse bone marrow chromosome aberration and micronucleus tests. Environ. Mol. Mutagen. 25, 302-313.
62. Sorsa, M., Autio, K., Abbondandolo, A., Carbonell, E., Demopoulos, N., Garner, C., Kirsch-Volders, M., Marcos, R., Marafante, E., Natarajan, A.T., Parry, E.M., Psaraki, K., Stephanou, G., Tales, A.D., Waters, R., 1992. Evaluation of in vitro cytogenetic techniques in nine European laboratories in relation to chromosomal endpoints induced by three model mutagens. Mutation Res. 271, 261-267.
63. Stopper, H., Eckert, I., Schiffmann, D., Spencer, D.L., Caspary, W.J., 1994a. Is micronucleus induction by aneugens an early event leading to mutagenesis?. Mutagenesis 9, 411-416.
64. Stopper, H., Kühnel, A., Podschun, B., 1994b. Combination of the chemotherapeutic agent 5-Fluorouracil with an inhibitor of its catabolism results in increased micronucleus induction. Biochem. Biophys. Res. Commun. 203 (Suppl. 2), 1124-1130.
65. Tokiwa, H., Ohnishi, Y., 1986. Mutagenicity and carcinogenicity of nitroarenes and their sources in the environment. CRC Crit. Rev. Toxicol. 17, 23-60.
66. Tsutsui, T., Maizumi, H., McLachlan, J.A., Barrett, J.C., 1983. Aneuploidy induction and cell transformation by diethylstilbestrol: a possible chromosomal mechanism in carcinogenesis. Cancer Res. 43, 3814-3821.
67. Tucker, R.W., Barrett, J.C., 1986. Decreased numbers of spindle and cytoplasmic microtubules in hamster embryo cells treated with carcinogen, diethylstilbestrol. Cancer Res. 46, 2088-2095.
68. Van Hummelen, P., Kirsch-Volders, M., 1992. Analysis of eight known or suspected aneugens by the in vitro human lymphoccyte micronucleus test. Mutagenesis 7, 447-455.
69. Vian, L., Bichet, N., Gouy, D., 1993. The in vitro micronucleus test on isolated human lymphocytes. Mutation Res. 291, 93-102.
70. Xu, W., Adler, I.D., 1990. Clastogenic effects of known and suspect spindle poisons studied by chromosome analysis in mouse bone marrow cells. Mutagenesis 5, 371-374.
71. Yager, J.W., Eastmond, D.A., Robertson, M.L., Paradisin, W.M., Smith, M.T., 1990. Characterization of micronuclei induced in HULY by benzene metabolites. Cancer Res. 50, 393-399.
72. Zhang, Li-Shi., Honma, M., Hayashi, M., Suzuki, T., Matsuoka, A., Sofuni, T., 1995. A comparative study of TK6 human lymphoblastoid and L5178Y mouse lymphoma cell lines in the in vitro micronucleus test. Mutation Res. 347, 105-115.
73. Zijno, A., Marcon, F., Leopardi, P., Crebelli, R., 1996. Analysis of chromosome segregation in cytokinesis blocked human lymphocytes: non-disjunction is the prevalent damage resulting from low concentration exposure to spindle poisons. Mutagenesis 11, 335-340.