Development of binding assays for the SH2 domain of Grb7 and Grb2 using fluorescence polarization

Journal of Biomolecular Screening

Volumn 13, Issue 2, 2008, Pages 112-119

  Luzy, Jean Philippe ^a   Huixiong Chen, ^a   Gril, Brunilde ^a   Liu, Wang Qing ^a   Vidal, Michel ^a   Perdereau, Dominique ^b   Burnol, Anne Françoise ^b   Garbay, Christiane ^a  

^a INSERM   (France)

^b UNIVERSITÉ PARIS DESCARTES   (France)

Author keywords

Assay development; Fluorescence polarization; Grb2; Grb7; Inhibitors; SH2 domain

Indexed keywords

AMIDE; ASPARAGINE DERIVATIVE; EPIDERMAL GROWTH FACTOR RECEPTOR 2; GROWTH FACTOR RECEPTOR BOUND PROTEIN 2; GROWTH FACTOR RECEPTOR BOUND PROTEIN 7; PROTEIN INHIBITOR; PROTEIN SH2; TYROSINE DERIVATIVE;

ARTICLE; BINDING AFFINITY; ENZYME LINKED IMMUNOSORBENT ASSAY; FLUORESCENCE POLARIZATION; PRIORITY JOURNAL; PROTEIN DETERMINATION; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN PHOSPHORYLATION; PROTEIN PROTEIN INTERACTION; PROTEIN TARGETING; REPRODUCIBILITY; VALIDATION PROCESS;

BINDING, COMPETITIVE; DIMETHYL SULFOXIDE; ENZYME-LINKED IMMUNOSORBENT ASSAY; FLUORESCENCE POLARIZATION IMMUNOASSAY; GRB2 ADAPTOR PROTEIN; GRB7 ADAPTOR PROTEIN; PROTEIN BINDING; SENSITIVITY AND SPECIFICITY; SRC HOMOLOGY DOMAINS; SUBSTRATE SPECIFICITY;

EID: 39049086229     PISSN: 10870571     EISSN: 1552454X     Source Type: Journal    
DOI: 10.1177/1087057107312124     Document Type: Article

References (31)

1. Margolis B., Silvennoinen O., Comoglio F., Roonprapunt C., Skolnik E., Ullrich A.,, et al. High-efficiency expression/cloning of epidermal growth factor-receptor-binding proteins with Src homology 2 domains. Proc Natl Acad Sci USA 1992 ; 89: 8894-8898.
2. Stein D., Wu J., Fuqua S., Roonprapunt C., Yajnik V., D'Eustachio P.,, et al. The SH2 domain protein GRB-7 is co-amplified, overexpressed and in a tight complex with HER2 in breast cancer. EMBO J 1994 ; 13: 1331-1340.
3. Janes PW, Lackmann M., Church WB, Sanderson GM, Sutherland RL, Daly RJ: Structural determinants of the interaction between the ErbB2 receptor and the Src homology 2 domain of Grb7. J Biol Chem 1997 ; 272: 8490-8497.
4. Fiddes RJ, Campbell DH, Janes PW, Sivertsen SP, Sasaki H., Wallasch C.,, et al. Analysis of Grb7 recruitment by heregulin-activated erbB receptors reveals a novel target selectivity for erbB3. J Biol Chem 1998 ; 273: 7717-7724.
5. Han DC, Guan JL: Association of focal adhesion kinase with Grb7 and its role in cell migration. J Biol Chem 1999 ; 274: 24425-24430.
6. Yokote K., Margolis B., Heldin CH, Claesson-Welsh L.: Grb7 is a downstream signalling component of platelet-derived growth factor alpha- and beta-receptors. J Biol Chem 1996 ; 271: 30942-30949.
7. Thommes K., Lennartsson J., Carlberg M., Ronnstrand L.: Identification of Tyr-703 and Tyr-936 as the primary association sites for Grb2 and Grb7 in the c-Kit/stem cell factor receptor. Biochem J 1999 ; 341: 211-216.
8. Pandey A., Liu X., Dixon JE, Di Fiore PP, Dixit VM: Direct association between the Ret receptor tyrosine kinase and the Src homology 2-containing adapter protein Grb7. J Biol Chem 1996 ; 271: 10607-10610.
9. Jones N., Master Z., Jones J., Bouchard D., Gunji Y., Sasaki H.,, et al. Identification of Tek/Tie2 binding partners: binding to a multifunctional docking site mediates cell survival and migration. J Biol Chem 1999 ; 274: 30896-30905.
10. Keegan K., Cooper JA: Use of the two hybrid system to detect the association of the protein-tyrosine-phosphatase, SHPTP2, with another SH2-containing protein, Grb7. Oncogene 1996 ; 12: 1537-1544.
11. Pero SC, Daly RJ, Krag DN: Grb7-based molecular therapeutics in cancer. Expert Rev Mol Med 2003 ; 5: 1-11.
12. Chen D., Xu LG, Chen L., Li L., Zhai Z., Shu HB: NIK is a component of the EGF/heregulin receptor signalling complexes. Oncogene 2003 ; 22: 4348-4355.
13. Han DC, Shen T-L., Guan J-L.: Role of Grb7 targeting to focal contacts and its phosphorylation by focal adhesion kinase in regulation of cell migration. J Biol Chem 2000 ; 275: 28911-28917.
14. Margolis B., Silvennoinen O., Comoglio F., Roonprapunt C., Skolnik E., Ullrich A.,, et al. Higher-efficiency expression/cloning of epidermal growth factor-receptor-binding proteins with Src homology 2 domains. Proc Natl Acad Sci USA 1992 ; 89: 8894-8898.
15. Pero SC, Oligino L., Daly RJ, Soden AL, Liu C., Roller PP,, et al. Identification of novel non-phosphorylated ligands, which bind selectively to the SH2 domain of Grb7. J Biol Chem 2002 ; 277: 11918-11926.
16. Tanaka S., Pero SC, Taguchi K., Shimada M., Mori M., Krag DN,, et al. Specific peptide ligand for Grb7 signal transduction protein and pancreatic cancer metastasis. J Natl Cancer Inst 2006 ; 98: 491-498.
17. Ivancic M., Spuches AM, Guth EC, Daugherty MA, Wilcox DE, Lyons BA: Backbone nuclear relaxation characteristics and calorimetric investigation of the human Grb7 SH2/ErbB2 peptide complex. Protein Sci 2005 ; 14: 1556-1569.
18. Jones RB, Gordus A., Krall JA, MacBeath G.: A quantitative protein interaction network for the ErbB receptors using protein microarrays. Nature 2006 ; 439: 168-174.
19. Owicki JC: Fluorescence polarization and anisotropy in high throughput screening: perspectives and primer. J Biomol Screen 2000 ; 5: 297-306.
20. Lynch BA, Loiacono KA, Tiong CL, Adams SE, MacNeil IA: A fluorescence polarization based Src-SH2 binding assay. Anal Biochem 1997 ; 247: 77-82.
21. Gril B., Liu WQ, Lenoir C., Garbay C., Vidal M.: Affinity chromatography for purification of the modular protein growth factor receptor-bound protein 2 and development of a screening test for growth factor receptor-bound protein 2 Src homology 3 domain inhibitor using peroxidase-linked ligand. Anal Biochem 2006 ; 351: 93-99.
22. Lohse J., Nielsen PE, Harrit N., Dahl O.: Fluorescein-conjugated lysine monomers for solid phase synthesis of fluorescent peptides and PNA oligomers. Bioconjugate Chem 1997 ; 8: 503-509.
23. Liu WQ, Vidal M., Gresh N., Roques BP, Garbay C.: Small peptides containing phosphotyrosine and adjacent alphaMe-phosphotyrosine or its mimetics as highly potent inhibitors of Grb2 SH2 domain. J Med Chem 1999 ; 42: 3737-3741.
24. Nikolovska-Coleska Z., Wang R., Fang X., Pan H., Tomita Y., Li P.,, et al. Development and optimization of a binding assay for the XIAP BIR3 domain using fluorescence polarization. Anal Biochem 2004 ; 332: 261-273.
25. Furet P., Gay B., García-Echeverría C., Rahuel J., Fretz H., Schoepfer J.,, et al. Discovery of 3-aminobenzyloxycarbonyl as an N-terminal group conferring high affinity to the minimal phosphopeptide sequence recognized by the Grb2 SH2 domain. J Med Chem 1997 ; 40: 3551-3556.
26. Ivancic M., Daly RJ, Lyons BA: Solution structure of the human Grb7 SH2 domain/ErbB2 peptide complex and structural basis for Grb7 binding to ErbB2. J Biomol NMR 2003 ; 27: 205-219.
27. Cheng YC, Prusoff WH: Relationship between the inhibition constant (KI) and the concentration of inhibitor which causes 50 per cent inhibition (I50) of an enzymatic reaction. Biochem Pharmacol 1973 ; 22: 3099-3108.
28. Cussac D., Frech M., Chardin P.: Binding of the Grb2 SH2 domain to phosphotyrosine motifs does not change the affinity of its SH3 domains for Sos proline-rich motifs. EMBO J 1994 ; 13: 4011-4021.
29. Liu WQ, Vidal M., Olszowy C., Million E., Lenoir C., Dhôtel H.,, et al. Structure-activity relationships of small phosphopeptides, inhibitors of Grb2 SH2 domain, and their prodrugs. J Med Chem 2004 ; 47: 1223-1233.
30. Zhang JH, Chung TD, Oldenburg KR: A simple statistical parameter for use in evaluation and validation of high throughput screening assays. J Biomol Screen 1999 ; 4: 67-73.
31. Gril B., Vidal M., Assayag F., Poupon MF, Liu WQ, Garbay C.: Grb2-SH3 ligand inhibits the growth of HER2(+) cancer cells and has antitumor effects in human cancer xenografts alone and in combination with docetaxel. Int J Cancer 2007 ; 121: 407-415.