Affinity chromatography for purification of the modular protein growth factor receptor-bound protein 2 and development of a screening test for growth factor receptor-bound protein 2 Src homology 3 domain inhibitor using peroxidase-linked ligand

Analytical Biochemistry

Volumn 351, Issue 1, 2006, Pages 93-99

  Gril, B ^a   Liu, W Q ^a   Lenoir, C ^a   Garbay, C ^a   Vidal, M ^a  

^a NONE

Author keywords

Affinity purification; Grb2; Inhibitor; Screening test; SH3

Indexed keywords

AFFINITY CHROMATOGRAPHY; LIGANDS; PEPTIDES;

AFFINITY PURIFICATION; CHEMICAL LIBRARIES; GRB2; GROWTH FACTOR RECEPTOR-BOUND PROTEIN 2; INHIBITOR; SCREENING TESTS; SRC HOMOLOGY 2 DOMAINS; SRC HOMOLOGY 3 DOMAINS;

PURIFICATION;

GROWTH FACTOR RECEPTOR BOUND PROTEIN 2; PEPTIDE DERIVATIVE; PEROXIDASE; PROLINE; PROTEIN; PROTEIN SH3; SEPHAROSE; UNCLASSIFIED DRUG; VALYLPROLYLPROLYLPROLYLVALYLPROLYLPROLYLARGINYLARGINYLARGININE;

AFFINITY CHROMATOGRAPHY; AMINO ACID SEQUENCE; ANALYTIC METHOD; ARTICLE; BINDING AFFINITY; CONCENTRATION RESPONSE; IC 50; MICROTITER PLATE ASSAY; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN EXPRESSION; PROTEIN PROTEIN INTERACTION; PROTEIN PURIFICATION; SCREENING TEST;

EID: 33644952203     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2005.12.032     Document Type: Article

References (26)

1. P. Chardin, D. Cussac, S. Maignan, and A. Ducruix The Grb2 adaptor FEBS Lett. 369 1995 47 51
2. S. Maignan, J.P. Guilloteau, N. Fromage, B. Arnoux, J. Becquart, and A. Ducruix Crystal structure of the mammalian Grb2 adaptor Science 268 1995 291 293
3. L. Buday, and J. Downward Epidermal growth factor regulates p21ras through the formation of a complex of receptor, Grb2 adapter protein, and Sos nucleotide exchange factor Cell 73 1993 611 620
4. M. Rozakis-Adcock, R. Fernley, J. Wade, T. Pawson, and D. Bowtell The SH2 and SH3 domains of mammalian Grb2 couple the EGF receptor to the Ras activator mSos1 Nature 363 1993 83 85
5. F. Huang, and A. Sorkin Growth factor receptor binding protein 2-mediated recruitment of the RING domain of Cbl to the epidermal growth factor receptor is essential and sufficient to support receptor endocytosis Mol. Biol. Cell 16 2005 1268 1281
6. X. Jiang, F. Huang, A. Marusyk, and A. Sorkin Grb2 regulates internalization of EGF receptors through clathrin-coated pits Mol. Biol. Cell 14 2003 858 870
7. S.J. Lim, G. Lopez-Berestein, M.C. Hung, R. Lupu, and A.M. Tari Grb2 downregulation leads to Akt inactivation in heregulin-stimulated and ErbB2-overexpressing breast cancer cells Oncogene 19 2000 6271 6276
8. S.G. Clark, M.J. Stern, and H.R. Horvitz C. elegans cell-signalling gene sem-5 encodes a protein with SH2 and SH3 domains Nature 356 1992 340 344
9. A.M. Cheng, T.M. Saxton, R. Sakai, S. Kulkarni, G. Mbamalu, W. Vogel, C.G. Tortorice, R.D. Cardiff, J.C. Cross, W.J. Muller, and T. Pawson Mammalian Grb2 regulates multiple steps in embryonic development and malignant transformation Cell 95 1998 793 803
10. P.W. Janes, R.J. Daly, A. deFazio, and R.L. Sutherland Activation of the Ras signalling pathway in human breast cancer cells overexpressing erbB-2 Oncogene 9 1994 3601 3608
11. A.M. Pendergast, L.A. Quilliam, L.D. Cripe, C.H. Bassing, Z. Dai, and N. Li BCR-ABL-induced oncogenesis is mediated by direct interaction with the SH2 domain of the GRB-2 adaptor protein Cell 75 1993 175 185
12. Y. Xie, A.M. Pendergast, and M.C. Hung Dominant-negative mutants of Grb2 induced reversal of the transformed phenotypes caused by the point mutation-activated rat HER-2/Neu J. Biol. Chem. 270 1995 30717 30724
13. D. Cussac, M. Vidal, C. Leprince, W.Q. Liu, F. Cornille, G. Tiraboschi, B.P. Roques, and C. Garbay A Sos-derived peptidimer blocks the Ras signaling pathway by binding both Grb2 SH3 domains and displays antiproliferative activity FASEB J. 13 1999 31 38
14. W.Q. Liu, M. Vidal, C. Olszowy, E. Million, C. Lenoir, H. Dhotel, and C. Garbay Structure-activity relationships of small phosphopeptides, inhibitors of Grb2 SH2 domain, and their prodrugs J. Med. Chem. 47 2004 1223 1233
15. B. Gay, S. Suarez, G. Caravatti, P. Furet, T. Meyer, and J. Schoepfer Selective Grb2 SH2 inhibitors as anti-Ras therapy Int. J. Cancer 83 1999 235 241
16. M. Vidal, W.Q. Liu, B. Gril, F. Assayag, M.F. Poupon, and C. Garbay Design of new anti-tumor agents interrupting deregulated signaling pathways induced by tyrosine kinase proteins: inhibition of protein-protein interaction involving Grb2 J. Soc. Biol. 198 2004 133 137
17. G.B. Fields, and R.L. Noble Solid phase peptide synthesis utilizing 9-fluorenylmethoxycarbonyl amino acids Int. J. Pept. Protein Res. 35 1990 161 214
18. D. Cussac, M. Frech, and P. Chardin Binding of the Grb2 SH2 domain to phosphotyrosine motifs does not change the affinity of its SH3 domains for Sos proline-rich motifs EMBO J. 13 1994 4011 4021
19. W.Q. Liu, M. Vidal, N. Gresh, B.P. Roques, and C. Garbay Small peptides containing phosphotyrosine and adjacent αMe-phosphotyrosine or its mimetics as highly potent inhibitors of Grb2 SH2 domain J. Med. Chem. 42 1999 3737 3741
20. J.L. Montiel, D. Cussac, F. Cornille, M. Vidal, C. Garbay, and B.P. Roques Rapid and efficient purification of rat brain dynamin using an affinity column of the carboxy-terminal SH3 domain of Grb2 Protein Pept. Lett. 4 1997 195 202
21. C. Cheadle, Y. Ivashchenko, V. South, G.H. Searfoss, S. French, R. Howk, G.A. Ricca, and M. Jaye Identification of an Src SH3 domain binding motif by screening a random phage display library J. Biol. Chem. 269 1994 24034 24039
22. A.B. Sparks, N.B. Adey, L.A. Quilliam, J.M. Thorn, and B.K. Kay Screening phage-displayed random peptide libraries for SH3 ligands Methods Enzymol. 255 1995 498 509
23. Y. Murakami, H. Fukazawa, T. Kobatake, S. Yamagoe, Y. Takebe, M. Tobiume, M. Matsuda, and Y. Uehara A mammalian two-hybrid screening system for inhibitors of interaction between HIV Nef and the cellular tyrosine kinase Hck Antiviral Res. 55 2002 161 168
24. B.A. Lynch, C. Minor, K.A. Loiacono, M.R. van Schravendijk, M.K. Ram, R. Sundaramoorthi, S.E. Adams, T. Phillips, D. Holt, R.J. Rickles, and I.A. MacNeil Simultaneous assay of Src SH3 and SH2 domain binding using different wavelength fluorescence polarization probes Anal. Biochem. 275 1999 62 73
25. S.R. Inglis, C. Stojkoski, K.M. Branson, J.F. Cawthray, D. Fritz, E. Wiadrowski, S.M. Pyke, and G.W. Booker Identification and specificity studies of small-molecule ligands for SH3 protein domains J. Med. Chem. 47 2004 5405 5417
26. M. Vidal, W.Q. Liu, C. Lenoir, J. Salzmann, N. Gresh, and C. Garbay Design of peptoid analogue dimers and measure of their affinity for Grb2 SH3 domains Biochemistry 43 2004 7336 7344