Differential intracellular distribution of DNA complexed with polyethylenimine (PEI) and PEI-polyarginine PTD influences exogenous gene expression within live COS-7 cells

Genetic Vaccines and Therapy

Volumn 5, Issue , 2007, Pages

  Doyle, Stephen R ^a   Chan, Chee Kai ^a  

^a LA TROBE UNIVERSITY   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

APHIDICOLIN; DNA; NOCODAZOLE; POLYARGININE; POLYETHYLENEIMINE;

ANIMAL CELL; ARTICLE; CELL NUCLEUS; CELL STRAIN COS7; CONTROLLED STUDY; FLUORESCENCE MICROSCOPY; GENE EXPRESSION; GENETIC TRANSFECTION; INTERNALIZATION; MICROTUBULE; MICROTUBULE ORGANIZING CENTER; MITOSIS; NONHUMAN; NONVIRAL GENE DELIVERY SYSTEM;

EID: 38549146997     PISSN: None     EISSN: 14790556     Source Type: Journal    
DOI: 10.1186/1479-0556-5-11     Document Type: Article

References (41)

1. Guillem VM Aliño SF Transfection pathways of nonspecific and targeted PEI-polyplexes Gene Therapy and Molecular Biology 2004, 8:369-384.
2. Lungwitz U Breunig M Blunk T Gopferich A Polyethylenimine-based non-viral gene delivery systems European Journal of Pharmaceutics & Biopharmaceutics 2005, 60:247-266. 10.1016/j.ejpb.2004.11.011
3. Florea BI Meaney C Junginger HE Borchard G Transfection efficiency and toxicity of polyethylenimine in differentiated Calu-3 and nondifferentiated COS-1 cell cultures AAPS PharmSci 2002, 4:E12. 10.1208/ ps040312 12423061
4. Zaric V Weltin D Erbacher P Remy JS Behr JP Stephan D Effective polyethylenimine-mediated gene transfer into human endothelial cells J Gene Med 2004, 6:176-184. 10.1002/jgm.484 14978771
5. Boussif O Lezoualc'h F Zanta MA Mergny MD Scherman D Demeneix B Behr JP A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: Polyethylenimine Proc Natl Acad Sci USA 1995, 92:7297-7301. 41326 7638184 10.1073/pnas.92.16.7297
6. Thomas M Lu JJ Zhang C Chen J Klibanov AM Full deacylation of polyethylenimine dramatically boosts its gene delivery efficiency and specificity to mouse lung Proc Natl Acad Sci USA 2005:5679-5684. 556302 15824322 10.1073/pnas.0502067102
7. Wightman L Kircheis R Rossler V Carotta S Ruzicka R Kursa M Wagner E Different behavior of branched and linear polyethylenimine for gene delivery in vitro and in vivo J Gene Med 2001, 3:362-372. 10.1002/ jgm.187 11529666
8. Godbey WT Barry MA Saggau P Wu KK Mikos AG Poly(ethylenimine)-mediated transfection: A new paradigm for gene delivery J Biomed Mater Res 2000, 51:321-328. 10.1002/1097-4636(20000905 51:3<321::AID-JBM5<3.0.CO;2-R 10880073
9. Kichler A Leborgne C Coeytaux E Danos O Polyethylenimine-mediated gene delivery: A mechanistic study J Gene Med 2001, 3:135-144. 10.1002/ jgm.173 11318112
10. Sonawane ND Szoka FC Jr Verkman AS Chloride accumulation and swelling in endosomes enhances DNA transfer by polyamine-DNA polyplexes J Biol Chem 2003, 278:44826-44831. 10.1074/jbc.M308643200 12944394
11. Suh J Wirtz D Hanes J Efficient active transport of gene nanocarriers to the cell nucleus Proc Natl Acad Sci USA 2003, 100:3878-3882. 153016 12644705 10.1073/pnas.0636277100
12. Kulkarni RP Wu DD Davis ME Fraser SE Quantitating intracellular transport of polyplexes by spatio-temporal image correlation spectroscopy Proc Natl Acad Sci USA 2005, 102:7523-7528. 1140437 15897455 10.1073/pnas.0501950102
13. Godbey WT Wu KK Mikos AG Tracking the intracellular path of poly(ethylenimine)/DNA complexes for gene delivery Proc Natl Acad Sci USA 1999, 96:5177-5181. 21837 10220439 10.1073/pnas.96.9.5177
14. Kichler A Gene transfer with modified polyethylenimines J Gene Med 2004, 6(Suppl 1):S3-10. 10.1002/jgm.507 14978746
15. Del Gaizo V Payne RM A novel TAT-Mitochondrial signal sequence fusion protein is processed, stays in mitochondria, and crosses the placenta Mol Ther 2003, 7:720-730. 10.1016/S1525-0016(03)00130-8 12788645
16. Li Y Rosal RV Brandt-Rauf PW Fine RL Correlation between hydrophobic properties and efficiency of carrier-mediated membrane transduction and apoptosis of a p53 C-terminal peptide Biochem Biophys Res Commun 2002, 298:439-449. 10.1016/S0006-291X(02)02470-1 12413961
17. Roeder GE Parish JL Stern PL Gaston K Herpes simplex virus VP22-human papillomavirus E2 fusion proteins produced in mammalian or bacterial cells enter mammalian cells and induce apoptotic cell death Biotechnol Appl Biochem 2004, 40:157-165. 10.1042/BA20030172 14709162
18. Shokolenko IN Alexeyev MF LeDoux SP Wilson GL TAT-mediated protein transduction and targeted delivery of fusion proteins into mitochondria of breast cancer cells DNA Repair (Amst) 2005, 4:511-518. 10.1016/ j.dnarep.2004.11.009 15725631
19. Matsui H Tomizawa K Lu YF Matsushita M Protein Therapy: In vivo protein transduction by polyarginine (11R) PTD and subcellular targeting delivery Curr Protein Pept Sci 2003, 4:151-157. 10.2174/1389203033487270 12678854
20. Dietz GP Kilic E Bahr M Inhibition of neuronal apoptosis in vitro and in vivo using TAT-mediated protein transduction Mol Cell Neurosci 2002, 21:29-37. 10.1006/mcne.2002.1165 12359149
21. Wadia JS Stan RV Dowdy SF Transducible TAT-HA fusogenic peptide enhances escape of TAT-fusion proteins after lipid raft macropinocytosis Nat Med 2004, 10:310-315. 10.1038/nm996 14770178
22. Amsellem S Pflumio F Bardinet D Izac B Charneau P Romeo PH Dubart-Kupperschmitt A Fichelson S Ex vivo expansion of human hematopoietic stem cells by direct delivery of the HOXB4 homeoprotein Nat Med 2003, 9:1423-1427. 10.1038/nm953 14578882
23. Mi Z Mai J Lu X Robbins PD Characterization of a class of cationic peptides able to facilitate efficient protein transduction in vitro and in vivo Mol Ther 2000, 2:339-347. 10.1006/mthe.2000.0137 11020349
24. Futaki S Suzuki T Ohashi W Yagami T Tanaka S Ueda K Sugiura Y Arginine-rich peptides. An abundant source of membrane-permeable peptides having potential as carriers for intracellular protein delivery J Biol Chem 2001, 276:5836-5840. 10.1074/jbc.M007540200 11084031
25. Suzuki T Futaki S Niwa M Tanaka S Ueda K Sugiura Y Possible existence of common internalization mechanisms among arginine-rich peptides J Biol Chem 2002, 277:2437-2443. 10.1074/jbc.M110017200 11711547
26. Takenobu T Tomizawa K Matsushita M Li ST Moriwaki A Lu YF Matsui H Development of p53 protein transduction therapy using membrane-permeable peptides and the application to oral cancer cells Mol Cancer Ther 2002, 1:1043-1049. 12481427
27. Matsushita M Tomizawa K Moriwaki A Li ST Terada H Matsui H A high-efficiency protein transduction system demonstrating the role of PKA in long-lasting long-term potentiation J Neurosci 2001, 21:6000-6007. 11487623
28. Richard JP Melikov K Vives E Ramos C Verbeure B Gait MJ Chernomordik LV Lebleu B Cell-penetrating peptides. A reevaluation of the mechanism of cellular uptake J Biol Chem 2003, 278:585-590. 10.1074/jbc.M209548200 12411431
29. Fuchs SM Raines RT Pathway for polyarginine entry into mammalian cells Biochemistry 2004, 43:2438-2444. 10.1021/bi035933x 14992581
30. Lundberg M Wikstrom S Johansson M Cell surface adherence and endocytosis of protein transduction domains Mol Ther 2003, 8:143-150. 10.1016/ S1525-0016(03)00135-7 12842437
31. Boeckle S Wagner E Ogris M C-versus N-terminally linked melittin-polyethylenimine conjugates: The site of linkage strongly influences activity of DNA polyplexes J Gene Med 2005, 7:1335-1347. 10.1002/jgm.783 15945120
32. Suh J Wirtz D Hanes J Real-time intracellular transport of gene nanocarriers studied by multiple particle tracking Biotechnol Prog 2004, 20:598-602. 10.1021/bp034251y 15059007
33. Escriou V Carriere M Bussone F Wils P Scherman D Critical assessment of the nuclear import of plasmid during cationic lipid-mediated gene transfer J Gene Med 2001, 3:179-187. 10.1002/jgm.174 11318117
34. Kircheis R Schuller S Brunner S Ogris M Heider KH Zauner W Wagner E Polycation-based DNA complexes for tumor-targeted gene delivery in vivo J Gene Med 1999, 1:111-120. 10.1002/(SICI)1521-2254(199903/ 04)1:2<111::AID-JGM22<3.0.CO;2-Y 10738575
35. Boeckle S von Gersdorff K van der Piepen S Culmsee C Wagner E Ogris M Purification of polyethylenimine polyplexes highlights the role of free polycations in gene transfer J Gene Med 2004, 6:1102-1111. 10.1002/ jgm.598 15386739
36. Akinc A Thomas M Klibanov AM Langer R Exploring polyethylenimine-mediated DNA transfection and the proton sponge hypothesis J Gene Med 2005, 7:657-663. 10.1002/jgm.696 15543529
37. Fittipaldi A Ferrari A Zoppe M Arcangeli C Pellegrini V Beltram F Giacca M Cell membrane lipid rafts mediate caveolar endocytosis of HIV-1 Tat fusion proteins J Biol Chem 2003, 278:34141-34149. 10.1074/ jbc.M303045200 12773529
38. Iwasa A Akita H Khalil I Kogure K Futaki S Harashima H Cellular uptake and subsequent intracellular trafficking of R8-liposomes introduced at low temperature Biochim Biophys Acta 2006, 1758:713-720. 10.1016/ j.bbamem.2006.04.015 16780792
39. Khalil IA Kogure K Akita H Harashima H Uptake pathways and subsequent intracellular trafficking in nonviral gene delivery Pharmacol Rev 2006, 58:32-45. 10.1124/pr.58.1.8 16507881
40. Bananis E Nath S Gordon K Satir P Stockert RJ Murray JW Wolkoff AW Microtubule-dependent movement of late endocytic vesicles in vitro: requirements for dynein and kinesin Mol Biol Cell 2004, 15:3688-3697. 491828 15181154 10.1091/mbc.E04-04-0278
41. Ikegami S Taguchi T Ohashi M Oguro M Nagano H Mano Y Aphidicolin prevents mitotic cell division by interfering with the activity of DNA polymerase-alpha Nature 1978, 275:458-460. 10.1038/275458a0 692726