Expression and enzymatic characterization of the soluble recombinant plasmepsin I from Plasmodium falciparum

Protein Engineering, Design and Selection

Volumn 20, Issue 12, 2007, Pages 625-633

  Xiao, Huogen ^a   Tanaka, Takuji ^b   Ogawa, Masahiro ^a,c   Yada, Rickey Y ^a  

^a UNIVERSITY OF GUELPH   (Canada)

^b UNIVERSITY OF SASKATCHEWAN   (Canada)

^c KAGAWA UNIVERSITY   (Japan)

Author keywords

Aspartic protease; Malaria; Plasmepsin I; Recombinant expression

Indexed keywords

ASPARTIC PROTEASE; RECOMBINANT EXPRESSION;

CELL CULTURE; ENZYME ACTIVITY; ESCHERICHIA COLI; LIQUID CHROMATOGRAPHY; PH EFFECTS;

MALARIA CONTROL;

HEMOGLOBIN; HYBRID PROTEIN; LEUCINE; LYSINE; NICKEL; PLASMEPSIN I; RECOMBINANT PLASMEPSIN I; SERINE; SYNTHETIC PEPTIDE; THIOREDOXIN; UNCLASSIFIED DRUG; VIRUS VECTOR;

ARTICLE; CELL CULTURE; CONTROLLED STUDY; DRUG SYNTHESIS; ESCHERICHIA COLI; GEL FILTRATION CHROMATOGRAPHY; GENE EXPRESSION; HUMAN; HYDROLYSIS; NONHUMAN; PH; PLASMODIUM FALCIPARUM; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DEGRADATION; PROTEIN PURIFICATION;

ANIMALS; ANTIGENS, PROTOZOAN; ASPARTIC ENDOPEPTIDASES; BINDING SITES; GENE EXPRESSION REGULATION; HEMOGLOBINS; HUMANS; HYDROGEN-ION CONCENTRATION; MODELS, MOLECULAR; PLASMODIUM FALCIPARUM; PROTEIN ENGINEERING; RECOMBINANT FUSION PROTEINS; RECOMBINANT PROTEINS; SUBSTRATE SPECIFICITY; THIOREDOXINS;

ESCHERICHIA COLI; PLASMODIUM FALCIPARUM;

EID: 37849000476     PISSN: 17410126     EISSN: 17410134     Source Type: Journal    
DOI: 10.1093/protein/gzm066     Document Type: Article

References (43)

1. Asojo,O.A., Afonina,E., Gulnik,S.V., Yu,B., Erickson,J.W., Randad,R., Medjahed,D. and Silva,A.M. (2002) Acta Cryst., 58, 2001-2008.
2. Asojo,O.A., Gulnik,S.V., Afonina,E., Yu,B., Ellman,J.A., Haque,T.S. and Silva,A.M. (2003) J. Mol. Biol., 327, 173-181.
3. Banerjee,R., Liu,J., Beaty,W., Pelosof,L., Klemba,M. and Goldberg,D.E. (2002) Proc. Natl Acad. Sci. USA, 99, 990-995.
4. Banerjee,R., Francis,S.E. and Goldberg,D.E. (2003) Mol. Biochem. Parasitol., 129, 157-165.
5. Berry,C., Humphreys,M.J., Matharu,P., Granger,R., Horrocks,P., Moon,R.P., Certa,U., Ridley,R.G., Bur,D. and Kay,J. (1999) FEBS Lett., 447, 149-154.
6. Boss,C., Richard-Bildstein,S., Weller,T., Fischli,W., Meyer,S. and Binkert,C. (2003) Curr. Med. Chem., 10, 883-907.
7. Campillo,E.D., Shannon,L.M. and Hankins,C.N. (1981) J. Biol. Chem., 256, 7177-7180.
8. Cheng,Y. and Prusoff,W.H. (1973) Biochem. Pharmacol., 22, 3099-3108.
9. Clemente,J.C., et al. (2006) Acta Crystallogr. D. Biol. Crystallogr., 62, 246-252.
10. Combet,C., Jambon,M., Deléage,G. and Geourjon,C. (2002) Bioinformatics, 18, 213-214.
11. Coombs,G.H., Goldberg,D.E., Klemba,M., Berry,C., Kay,J. and Mottram,J.C. (2001) Trends Parasitol., 17, 532-537.
12. Ersmark,K., Samuelsson,B. and Hallberg,A. (2006) Med. Res. Rev., 26, 626-666.
13. Francis,S.E., Gluzman,I.Y., Oksman,A., Banerjee,D. and Goldberg,D.E. (1996) Mol. Biochem. Parasitol., 83, 189-200.
14. Francis,S.E., Banerjee,R. and Goldberg,D.E. (1997) J. Biol. Chem., 272, 14961-14968.
15. Fruton,J.S. (1971) In Boyer,P.D. (eds.), The Enzyme. Academic Press, New York, pp. 119-164.
16. Gluzman,I.Y., Francis,S.E., Oksman,A., Smith,C.E., Duffin,K.L. and Goldberg,D.E. (1994) J. Clin. Invest., 93, 1602-1608.
17. Goldberg,D.E., Slater,A.F., Beavis,R., Chait,B., Cerami,A. and Herderson,G.B. (1991) J. Exp. Med., 173, 961-969.
18. Greenwood,B.M., Bojang,K., Whitty,C.J. and Targett,G.A. (2005) Lancet, 365, 1487-1498.
19. Gutierrez-de-Teran,H., Nervall,M., Ersmark,K., Liu,P., Janka,L.K., Bunn,B., Hallberg,A. and Aqvis,J. (2006) Biochemistry, 45, 10529-10541.
20. Hill,J., Tyas,L., Phylip,L.H., Dunn,B.M. and Berry,C. (1994) FEBS Lett., 26, 155-158.
21. Humphreys,M.J., Moon,R.P., Klinder,A., Fowler,S.D., Rupp,K., Bur,D., Ridley,R.G. and Berry,C. (1999) FEBS Lett., 463, 43-48.
22. Istvan,E.S. and Goldberg,D.E. (2005) J. Biol. Chem., 280, 6890-6896.
23. Johansson,P.O., et al. (2004) J. Med. Chem., 47, 3353-3366.
24. Kale,L., Skeel,R., Bhandarkar,M., Brunner,R., Gursoy,A., Krawetz,N., Phillips,J., Shinozaki,A., Varadarajan,K. and Schulten,K. (1999) J. Comp. Phys., 151, 283-312.
25. Kim,Y.M., Lee,M.H., Piao,T.G., Lee,J.W., Kim,J.H., Lee,S., Choi,K.M., Jiang,J.H., Kim,T.U. and Park,H. (2006) J. Biochem., 139, 189-195.
26. Klemba,M. and Goldberg,D.E. (2005) Mol. Biochem. Parasitol., 143, 183-191.
27. Klyachko,N.L., Shchedrina,V.A., Efimov,A.V., Kazakov,S.V., Gazayan,I.G., Kristal,B.S. and Brown,A.M. (2005) J. Biol. Chem., 280, 16106-16114.
28. Kolakovich,K.A., Gluzman,I.Y., Duffin,K.L. and Goldberg,D.E. (1997) Mol. Biochem. Parasitol., 87, 123-135.
29. Laemmli,U.K. (1970) Nature, 227, 680-685.
30. Li,T., Yowell,C.A., Beyer,B.B., Hung,S.-H., Westling,J., Lam,M.T., Dunn,B.M. and Dame,J.B. (2004) Mol. Biochem. Parasitol., 135, 101-109.
31. Lowry,O.H., Rosebrough,N.J., Farr,A.L. and Randall,R.J. (1951) J. Biol. Chem., 193, 265-275.
32. Luker,K.E., Francis,S.E., Gluzman,I.Y. and Goldberg,D.E. (1996) Mol. Biochem. Parasitol., 79, 71-78.
33. Moon,R.P., et al. (1997) Eur. J. Biochem., 244, 552-560.
34. Olliaro,P.L. and Goldberg,D.E. (1995) Parasitol. Today, 11, 294-297.
35. Ridley,R.G. (2002) Nature, 415, 686-693.
36. Salas,F., Fichmann,J., Lee,G.K., Scott,M.D. and Rosenthal,P.J. (1995) Infect. Immun., 63, 2120-2125.
37. Sijwali,P.S., Shenai,B.R., Gut,J., Singh,A. and Rosenthal,P.J. (2001) Biochem. J., 360, 481-489.
38. Silva,A.M., et al. (1996) Proc. Natl Acad. Sci. USA, 93, 10034-10039.
39. Towbin,H., Staehelin,T. and Gordon,J. (1979) Proc. Natl Acad. Sci. USA, 76, 4350-4354.
40. Tyas,L., Gluzman,I., Moon,R.P., Rupp,K., Westling,J., Ridley,R.G., Kay,J., Goldberg,D.E. and Berry,C. (1999) FEBS Lett., 454, 210-214.
41. Wong,J.H., Yee,B.C. and Buchanan,B.B. (1987) J. Biol. Chem., 262, 3185-3191.
42. Xiao,H., Sinkovits,A.F., Bryksa,B.C., Ogawa,M. and Yada,R.Y. (2006) Protein Expr. Purif., 49, 88-94.
43. Yayon,A., Cabantchik,Z.I. and Ginsburg,H. (1984) EMBO J., 3, 2695-2700.