Recombinant expression and partial characterization of an active soluble histo-aspartic protease from Plasmodium falciparum

Protein Expression and Purification

Volumn 49, Issue 1, 2006, Pages 88-94

  Xiao, Huogen ^a   Sinkovits, Andras F ^a   Bryksa, Brian C ^a   Ogawa, Masahiro ^a   Yada, Rickey Y ^a  

^a UNIVERSITY OF GUELPH   (Canada)

Author keywords

Expression; Histo aspartic protease; Malaria; Recombinant; Soluble

Indexed keywords

ASPARTIC PROTEINASE; GLOBIN; HYBRID PROTEIN; THIOREDOXIN;

ANIMAL; ARTICLE; CATALYSIS; ENZYME ACTIVATION; ENZYMOLOGY; GENE EXPRESSION; GENETICS; ISOLATION AND PURIFICATION; KINETICS; METABOLISM; PH; PLASMODIUM FALCIPARUM; SOLUBILITY;

ANIMALS; ASPARTIC ENDOPEPTIDASES; CATALYSIS; ENZYME ACTIVATION; GENE EXPRESSION; GLOBINS; HYDROGEN-ION CONCENTRATION; KINETICS; PLASMODIUM FALCIPARUM; RECOMBINANT FUSION PROTEINS; SOLUBILITY; THIOREDOXIN;

ESCHERICHIA COLI; PLASMODIUM FALCIPARUM;

EID: 33747750905     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2006.02.022     Document Type: Article

References (26)

1. Olliaro P.L., and Goldberg D.E. The Plasmodium digestive vacuole: metabolic headquarters and choice drug target. Parasitol. Today 11 (1995) 294-297
2. Silva A.M., Lee A.Y., Gulnik S.V., Maier P., Collins J., Bhat T.N., Collins P.J., Cachau R.E., Luker K.E., Gluzman I.Y., Francis S.E., Oksman A., Goldberg D.E., and Erickson J.W. Structure and inhibition of plasmepsin II, a hemoglobin-degrading-enzyme enzyme from Plasmodium falciparum. Proc. Natl. Acad. Sci. USA 93 (1996) 10034-10039
3. Jiang S., Prigge S.T., Wei L., Gao Y., Hudson T.H., Gerena L., Dame J.B., and Kyle D.E. New class of small nonpeptidyl compounds blocks Plasmodium falciparum development in vitro by inhibiting plasmepsins. Antimicrob. Agents Chemother 45 (2001) 2577-2584
4. Asojo O.A., Afonina E., Gulnik S.V., Yu B., Erickson J.W., Randad R., Medjahed D., and Silva A.M. Structures of Ser205 mutant plasmepsin II from Plasmodium falciparum at 1.8 Å in complex with the inhibitors rs367 and rs370. Acta Cryst. 58 (2002) 2001-2008
5. Asojo O.A., Gulnik S.V., Afonina E., Yu B., Ellman J.A., Haque T.S., and Silva A.M. Novel uncomplexed and complexed structures of plasmepsin II, an aspartic protease from Plasmodium falciparum. J. Mol. Biol. 327 (2003) 173-181
6. Nezami A., Kimura T., Hidaka K., Kiso A., Liu J., Kiso Y., Goldberg D.E., and Freire E. High-affinity inhibition of a family of Plasmodium falciparum proteases by a designed daptive inhibitor. Biochemistry 42 (2003) 8459-8464
7. Boss C., Richard-Bildstein S., Weller T., Fischli W., Meyer S., and Binkert C. Inhibitors of the Plasmodium falciparum parasite aspartic protease plasmepsin II as potential antimalarial agents. Curr. Med. Chem. 10 (2003) 883-907
8. Dahlgren A., Kvarnstron I., Vrang L., Hamelink E., Hallberg A., Rosenquist A., and Samuelsson B. New inhibitors of the malaria aspartyl proteases plasmepsin I and II. Bioorg. Med. Chem. 11 (2003) 3423-3437
9. Ersmark K., Feierberg I., Bjelic S., Hulten J., Samuelsson B., Aqvist J., and Hallberg A. C2-symmetric inhibitors of Plasmodium falciparum plasmepsin II: synthesis and theoretical prediction. Bioorg. Med. Chem. 11 (2003) 3723-3733
10. Johansson P.O., Chen Y., Belfrage A.K., Blackman M.J., Kyarnstrom I., Jansson K., Vrang L., Hamelink E., Hallberg A., Rosenquist A., and Samuelsson B. Design and synthesis of potent inhibitors of the malaria aspartyl proteases plasmepsin I and II. Use of solid-phase synthesis to explore novel statine motifs. J. Med. Chem. 47 (2004) 3353-3366
11. Coombs G.H., Goldberg D.E., Klemba M., Berry C., Kay J., and Mottram J.C. Aspartic protease of Plasmodium falciparum and other parasitic protozoa as drug targets. Trends Parasitol. 17 (2001) 532-537
12. Banerjee R., Liu J., Beaty W., Pelosof L., Klemba M., and Goldberg D.E. Four plasmepsins are active in the Plasmodium falciparum food vacuole, including a protease with an active-site histidine. PNAS 99 2 (2002) 990-995
13. Klemba M., and Goldberg D.E. Characterization of plasmepsin V, a membrane-bound aspartic protease homolog in the endoplasmic reticulum of Plasmodium falciparum. Mol. Biochem. Parasitol. 143 2 (2005) 183-191
14. Berry C., Humphreys M.J., Matharu P., Granger R., Horrocks P., Moon R.P., Certa U., Ridley R.G., Bur D., and Kay J. A distinct member of the aspartic proteinase gene family from the human malaria parasite Plasmodium falciparum. FEBS Lett. 447 (1999) 149-154
15. Loll P.J. Membrane protein structural biology: the high throughput challenge. J. Struct. Biol. 142 (2003) 144-153
16. LaCount D.J., Vignali M., Chettier R., Phansalkar A., Bell R., Hesselberth J.R., Schoenfeld L.W., Ota I., Sahasrabudhe S., Kurschner C., Fields S., and Hughes R.E. A protein interaction network of the malaria parasite Plasmodium falciparum. Nature Lett. 438 3 (2005) 103-107
17. Andreeva N., Bogdanovich P., Kashparov I., Popov M., and Stengach M. Is histo-aspartic protease a serine protease with a pepsin-like fold?. Proteins: Struct. Funct. and Bioinf. 55 (2004) 705-710
18. Bjelic S., and Aqvist J. Computational prediction of structure, substrate binding mode, mechanism, and rate for a malaria protease with a novel type of active site. Biochemistry 43 (2004) 14521-14528
19. Moon R.P., Tyas L., Certa U., Rupp K., Bur D., Jaquet C., Matile H., Loetscher H.R., Grueninger F., Kay J., Dunn B.M., Berry C., and Ridley R.G. Expression and characterisation of plasmepsin I from Plasmodium falciparum. Eur. J. Biochem. 244 (1997) 552-560
20. Hill J., Tyas L., Phylip L.H., Dunn B.M., and Berry C. High level expression and characterisation of Plasmepsin II, an aspartic proteinase from Plasmodium falciparum. FEBS Lett. 26 2 (1994) 155-158
21. Rossi A., Antonini E., and Caputo A. Studies on the structure of hemoglobin I. Physicochemical properties of human globin. Biochim. Biophys. Acta 30 (1958) 608-615
22. Istvan E.S., and Goldberg D.E. Distal substrate interactions enhance plasmepsin activity. J. Biol. Chem. 280 (2005) 6890-6896
23. Grisshammer R., and Tate C.G. Overexpression of integral membrane proteins for structural studies. Q. Rev. Biophys. 28 3 (1995) 315-422
24. Banerjee R., Francis S.E., and Goldberg D.E. Food vacuole plasmepsins are processed at a conserved site by an acidic convertase activity in Plasmodium falciparum. Mol. Biochem. Parasitol. 129 (2003) 157-165
25. Tyas L., Gluzman I., Moon R.P., Rupp K., Westling J., Ridley R.G., Kay J., Goldberg D.E., and Berry C. Naturally-occurring and recombinant forms of the aspartic proteinases plasmepsins I and II from the human malaria parasite Plasmodium falciparum. FEBS Lett. 454 (1999) 210-214
26. Luker K.E., Francis S.E., Gluzman I.Y., and Goldberg D.E. Kinetic analysis of plasmepsins I and II aspartic proteases of the Plasmodium falciparum digestive vacuole. Mol. Biochem. Parasitol. 79 (1996) 71-78