Staurosporine-based binding assay for testing the affinity of compounds to protein kinases

Analytical Biochemistry

Volumn 373, Issue 2, 2008, Pages 197-206

  Iyer, Ganesh H ^a   Taslimi, Paul ^a   Pazhanisamy, S ^a  

^a VERTEX PHARMACEUTICALS   (United States)

Author keywords

Binding assay; Competitive ligand displacement; Dissociation constant; Fluorescence; Protein kinase; Staurosporine

Indexed keywords

ADENOSINETRIPHOSPHATE; AMINO ACIDS; BINDING ENERGY; DISSOCIATION; FLUORESCENCE; PHOSPHORYLATION;

BINDING ASSAYS; DISSOCIATION CONSTANT; LIGAND DISPLACEMENT; PROTEIN KINASE; STAUROSPORINES;

ENZYMES;

ADENOSINE TRIPHOSPHATASE; PHOSPHOPROTEIN; PROTEIN KINASE; STAUROSPORINE;

ARTICLE; BINDING AFFINITY; BINDING ASSAY; DISSOCIATION CONSTANT; DRUG SCREENING; FLUORESCENCE; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION; PROTEIN SYNTHESIS; X RAY CRYSTALLOGRAPHY;

EID: 37548999004     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2007.11.004     Document Type: Article

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