Comparative analysis of the human angiotensin II type 1a receptor heterologously produced in insect cells and mammalian cells

Biochemical and Biophysical Research Communications

Volumn 349, Issue 1, 2006, Pages 6-14

  Shukla, Arun Kumar ^a   Reinhart, Christoph ^a   Michel, Hartmut ^a  

^a MAX PLANCK INSTITUTE OF BIOPHYSICS   (Germany)

Author keywords

Angiotensin receptor; BHK cells; Glycosylation; GPCR; Insect cells; Localization; Overexpression

Indexed keywords

ANGIOTENSIN 1A RECEPTOR; ANGIOTENSIN 2 RECEPTOR; G PROTEIN COUPLED RECEPTOR; GUANINE NUCLEOTIDE BINDING PROTEIN; MEMBRANE PROTEIN; MITOGEN ACTIVATED PROTEIN KINASE;

ANIMAL CELL; ARTICLE; BACULOVIRUS EXPRESSION SYSTEM; BINDING AFFINITY; BLOOD PRESSURE REGULATION; COMPARATIVE STUDY; GENE OVEREXPRESSION; GLYCOSYLATION; HETEROLOGOUS EXPRESSION; HORMONE RELEASE; INSECT CELL; INTERNALIZATION; KIDNEY FUNCTION; LASER MICROSCOPY; MAMMAL CELL; NONHUMAN; PRIORITY JOURNAL; PROTEIN LOCALIZATION; RECEPTOR BINDING; SEMLIKI FOREST ALPHAVIRUS; VASCULAR SMOOTH MUSCLE; VIRUS CHARACTERIZATION;

ANIMALS; CELL MEMBRANE; CRICETINAE; GENETIC TECHNIQUES; GLYCOSYLATION; GTP-BINDING PROTEIN ALPHA SUBUNITS, GQ-G11; HUMANS; INSECTS; MICROSCOPY, CONFOCAL; PROTEIN STRUCTURE, TERTIARY; RECEPTOR, ANGIOTENSIN, TYPE 1; RECOMBINANT PROTEINS; SEMLIKI FOREST VIRUS;

INSECTA; MAMMALIA; SEMLIKI FOREST VIRUS;

EID: 33748288247     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2006.07.210     Document Type: Article

References (40)

1. Peach M.J. Renin-angiotensin system: biochemistry and mechanisms of action. Physiol. Rev. 57 (1977) 313-370
2. Peach M.J., and Dostal D.E. The angiotensin II receptor and the actions of angiotensin II. J. Cardiovasc. Pharmacol. 16 (1990) 25-30
3. Leung P.S. The peptide hormone angiotensin II: its new functions in tissues and organs. Curr. Protein Pept. Sci. 5 (2004) 267-273
4. de Gasparo M., Catt K.J., Inagami T., Wright J.W., and Unger T. International union of pharmacology. XXIII. The angiotensin II receptors. Pharmacol. Rev. 52 3 (2000) 415-472 Review
5. Grone H.J., Simon M., and Fuchs E. Autoradiographic characterization of angiotensin receptor subtypes in fetal and adult human kidney. Am. J. Physiol. 262 (1992) 326-331
6. Nickenig G., Geisen G., Vetter H., and Sachinidis A. Characterization of angiotensin receptors on human skin fibroblasts. J. Mol. Med. 75 (1997) 217-222
7. Lanctot P.M., Leclerc P.C., Escher E., Leduc R., and Guillemette G. Role of N-glycosylation in the expression and functional properties of human AT1 receptor. Biochemistry 38 (1999) 8621-8627
8. Le M.T., De Backer J.P., Hunyady L., Vanderheyden P.M., and Vauquelin G. Ligand binding and functional properties of human angiotensin AT1 receptors in transiently and stably expressed CHO-K1 cells. Eur. J. Pharmacol. 513 (2005) 35-45
9. Sarramegna V., Talmont F., Demange P., and Milon A. Heterologous expression of G-protein-coupled receptors: comparison of expression systems fron the standpoint of large-scale production and purification. Cell. Mol. Life Sci. 60 (2003) 1529-1546
10. Massotte D. G protein-coupled receptor overexpression with the baculovirus-insect cell system: a tool for structural and functional studies. Biochim. Biophys. Acta 17 (2003) 77-89
11. Akermoun M., Koglin M., Zvalova-Iooss D., Folschweiller N., Dowell S.J., and Gearing K.L. Characterization of 16 human G protein-coupled receptors expressed in baculovirus-infected insect cells. Protein Expr. Purif. 44 (2005) 65-74
12. Hassaine G., Wagner R., Kempf J., Cherouati N., Hassaine N., Prual C., Andre N., Reinhart C., Pattus F., and Lundstrom K. Semliki Forest virus vectors for overexpression of 101 G protein-coupled receptors in mammalian host cells. Protein Expr. Purif. 45 (2005) 343-351
13. Shukla A.K., Haase W., Reinhart C., and Michel H. Biochemical and pharmacological characterization of the human bradykinin subtype 2 receptor produced in mammalian cells using the Semliki Forest virus system. Biol. Chem. 387 5 (2006) 569-576
14. Shukla A.K., Reinhart C., and Michel H. Dimethylsulphoxide as a tool to increase functional expression of heterologously produced GPCRs in mammalian cells. FEBS Lett. 580 17 (2006) 4261-4265
15. A.K. Shukla, W. Haase, C. Reinhart, H. Michel, Functional overexpression and characterization of human bradykinin subtype 2 receptor in insect cells using the baculovirus system, J. Cell. Biochem. (2006) in press.
16. Cronan J.E. Biotination of proteins in vivo. A post-translational modification to label, purify, and study proteins. J. Biol. Chem. 265 (1990) 10327-10333
17. Lundstrom K. Semliki Forest virus vectors for large-scale production of recombinant proteins. In: Machida C.A. (Ed). Viral Vectors for Gene Therapy: Methods and Protocols (2003), Human Press, New Jersy 76-87
18. Lundstrom K., Schweitzer C., Rotmann D., Hermann D., Schneider E.M., and Ehrengruber M.U. Semliki forest virus vectors: efficient vehicles for in vitro and in vivo gene delivery. FEBS lett. 504 (2001) 99-103
19. O'Reilly D.R., Miller L.K., and Luckow W.A. Baculovirus Expression Vectors: A Laboratory Manual (1992), W.H. Freeman, New York
20. Reed L.J., and Muench H. A simple method of estimating fifty percent endpoints. Am. J. Hygiene 27 (1938) 493-497
21. Gimpl G., Klein U., Reilander H., and Fahrenholz F. Expression of the human oxytocin receptor in baculovirus-infected insect cells: high-affinity binding is induced by a cholesterolcyclodextrin complex. Biochemistry 34 (1995) 13794-13801
22. Lenhard T., Maul G., Haase W., and Reilander H. A new set of versatile vectors for the heterologous expression of foreign genes using the baculovirus system. Gene 169 (1996) 1871-1890
23. Grunewald S., Reilander H., and Michel H. In vivo reconstitution of dopamine D2S receptor-mediated G protein activation in baculovirus-infected insect cells: preferred coupling to Gi1 versus Gi2. Biochemistry 35 (1996) 15162-15173
24. Sjoberg E.M., Suomalainen M., and Garoff H. A significantly improved semliki forest virus expression system based on the translation enhancer segments from the viral capsid gene. BioTechnology 12 (1994) 1127-1131
25. Grunewald S., Haase W., Molsberger E., Michel H., and Reilander H. Production of the human D2S receptor in the methylotrophic yeast P. pastoris. Receptors Channels 10 (2004) 37-50
26. Hampe W., Voss R.H., Haase W., Boege F., Michel H., and Reilander H. Engineering of a proteolytically stable human beta 2-adrenergic receptor/maltose-binding protein fusion and production of the chimeric protein in Escherichia coli and baculovirus-infected insect cells. J. Biotechnol. 77 (2000) 219-234
27. Ratnala V.R., Swarts H.G., VanOostrum J., Leurs R., DeGroot H.J., Bakker R.A., and DeGrip W.J. Large-scale overproduction, functional purification and ligand affinities of the His-tagged human histamine H1 receptor. Eur. J. Biochem. 271 (2004) 2636-2646
28. Maeda Y., Kuroki R., Haase W., Michel H., and Reilander H. Comparative analysis of high-affinity ligand binding and G protein coupling of the human CXCR1 chemokine receptor and of a CXCR1-Galpha fusion protein after heterologous production in baculovirus-infected insect cells. Eur. J. Biochem. 271 (2004) 1677-1689
29. Lson J.M., Kennedy S.J., and Cabral G.A. Expression of the murine CB2 cannabinoid receptor using recombinant semliki forest virus. Biochem. Pharmacol. 65 (2003) 1931-1942
30. Vasudevan S., Hulme E.C., Bach M., Haase W., Pavia J., and Reilander H. Characterization of the rat m3 muscarinic acetylcholine receptor produced in insect cells infected with recombinant baculovirus. Eur. J. Biochem. 15 (1995) 466-475
31. Blaukat A., Herzer K., Schroeder C., Bachmann M., Nash N., and Muller-Esterl W. Overexpression and functional characterization of kinin receptors reveal subtype-specific phosphorylation. Biochemistry 38 (1999) 1300-1309
32. Dolby V., Collen A., Lundqvist A., and Cronet P. Overexpression and functional characterisation of the human melanocortin 4 receptor in Sf9 cells. Protein Expr. Purif. 37 (2004) 455-461
33. Kempf J., Snook L.A., Vonesch J.L., Dahms T.E., Pattus F., and Massotte D. Expression of the human mu opioid receptor in a stable Sf9 cell line. J. Biotechnol. 95 (2002) 181-187
34. Michineau S., Muller L., Pizard A., Alhenc-Gelas F., and Rajerison R.M. N-linked glycosylation of the human bradykinin B2 receptor is required for optimal cell-surface expression and coupling. Biol. Chem. 385 (2004) 49-57
35. Davis D., Liu X., and Segaloff D.L. Identification of the sites of N-linked glycosylation on the follicle-stimulating hormone (FSH) receptor and assessment of their role in FSH receptor function. Mol. Endocrinol. 9 (1995) 159-170
36. Rands E., Candelore M.R., Cheung A.H., Hill W.S., Strader S.D., and Dixon R.A. Mutational analysis of beta-adrenergic receptor glycosylation. J. Biol. Chem. 265 (1990) 10759-10764
37. Fukushima Y., Oka Y., Saitoh T., Katagiri H., Asano T., Matsuhashi N., Takata K., van Breda E., Yazaki Y., and Sugano K. Structural and functional analysis of the canine histamine H2 receptor by site-directed mutagenesis: N-glycosylation is not vital for its action. Biochem. J. 310 (1995) 553-558
38. van Koppen C.J., and Nathanson N.M. Site-directed mutagenesis of the m2 muscarinic acetylcholine receptor. Analysis of the role of N-glycosylation in receptor expression and function. J. Biol. Chem. 265 (1990) 20887-20892
39. Sawutz D.G., Lanier S.M., Warren S.M., and Graham R.M. Glycosylation of the mammalian alpha 1-adrenergic receptor by complex type N-linked oligosaccharides. Mol. Pharmacol. 32 (1987) 565-571
40. Kojima I., Kojima K., Kreutter D., and Rasmussen H. The temporal integration of the aldosterone secretory response to angiotensin occurs via two intracellular pathways. J. Biol. Chem. 259 (1984) 14448-14457