Rad52 multimerization is important for its nuclear localization in Saccharomyces cerevisiae

DNA Repair

Volumn 7, Issue 1, 2008, Pages 57-66

  Plate, Iben ^a   Albertsen, Line ^a   Lisby, Michael ^b   Hallwyl, Swee C L ^a   Feng, Qi ^c   Seong, Changhyun ^d   Rothstein, Rodney ^c   Sung, Patrick ^d   Mortensen, Uffe H ^a  

^a TECHNICAL UNIVERSITY OF DENMARK   (Denmark)

^b UNIVERSITY OF COPENHAGEN   (Denmark)

^c NewYork Presbyterian Hospital   (United States)

^d YALE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

DNA repair; Homologous recombination; NLS; Nuclear localization; Rad52; S. cerevisiae

Indexed keywords

RAD52 PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; CONTROLLED STUDY; DNA REPAIR; DNA STRAND BREAKAGE; HOMOLOGOUS RECOMBINATION; MUTAGENESIS; NONHUMAN; NUCLEAR LOCALIZATION SIGNAL; PRIORITY JOURNAL; PROTEIN FUNCTION; PROTEIN INTERACTION; PROTEIN POLYMERIZATION; PROTEIN STRUCTURE; PROTEIN TRANSPORT; SACCHAROMYCES CEREVISIAE; SEQUENCE ANALYSIS;

AMINO ACID SEQUENCE; BIOPOLYMERS; CELL NUCLEUS; DNA DAMAGE; MICROSCOPY, FLUORESCENCE; MOLECULAR SEQUENCE DATA; NUCLEAR LOCALIZATION SIGNALS; PLASMIDS; RAD52 DNA REPAIR AND RECOMBINATION PROTEIN; SACCHAROMYCES CEREVISIAE; SEQUENCE HOMOLOGY, AMINO ACID;

SACCHAROMYCES CEREVISIAE;

EID: 36549077017     PISSN: 15687864     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.dnarep.2007.07.016     Document Type: Article

References (42)

1. Symington L.S. Role of RAD52 epistasis group genes in homologous recombination and double-strand break repair. Microbiol. Mol. Biol. Rev. 66 (2002) 630-670 table
2. Mortensen U.H., Bendixen C., Sunjevaric I., and Rothstein R. DNA strand annealing is promoted by the yeast Rad52 protein. Proc. Natl. Acad. Sci. U.S.A. 93 (1996) 10729-10734
3. Sugiyama T., New J.H., and Kowalczykowski S.C. DNA annealing by RAD52 protein is stimulated by specific interaction with the complex of replication protein A and single-stranded DNA. Proc. Natl. Acad. Sci. U.S.A. 95 (1998) 6049-6054
4. Kagawa W., Kurumizaka H., Ikawa S., Yokoyama S., and Shibata T. Homologous pairing promoted by the human Rad52 protein. J. Biol. Chem. 276 (2001) 35201-35208
5. Sung P. Function of yeast Rad52 protein as a mediator between replication protein A and the Rad51 recombinase. J. Biol. Chem. 272 (1997) 28194-28197
6. New J.H., Sugiyama T., Zaitseva E., and Kowalczykowski S.C. Rad52 protein stimulates DNA strand exchange by Rad51 and replication protein A. Nature 391 (1998) 407-410
7. Shen Z., Peterson S.R., Comeaux J.C., Zastrow D., Moyzis R.K., Bradbury E.M., et al. Self-association of human RAD52 protein. Mutat. Res. 364 (1996) 81-89
8. Shinohara A., Shinohara M., Ohta T., Matsuda S., and Ogawa T. Rad52 forms ring structures and co-operates with RPA in single-strand DNA annealing. Genes Cells 3 (1998) 145-156
9. Van D.E., Hajibagheri N.M., Stasiak A., and West S.C. Visualisation of human rad52 protein and its complexes with hRad51 and DNA. J. Mol. Biol. 284 (1998) 1027-1038
10. Davis A.P., and Symington L.S. The yeast recombinational repair protein Rad59 interacts with Rad52 and stimulates single-strand annealing. Genetics 159 (2001) 515-525
11. Cortes-Ledesma F., Malagon F., and Aguilera A. A novel yeast mutation, rad52-L89F, causes a specific defect in Rad51-independent recombination that correlates with a reduced ability of Rad52-L89F to interact with Rad59. Genetics 168 (2004) 553-557
12. Ranatunga W., Jackson D., Lloyd J.A., Forget A.L., Knight K.L., and Borgstahl G.E. Human RAD52 exhibits two modes of self-association. J. Biol. Chem. 276 (2001) 15876-15880
13. Milne G.T., and Weaver D.T. Dominant negative alleles of RAD52 reveal a DNA repair/recombination complex including Rad51 and Rad52. Genes Dev. 7 (1993) 1755-1765
14. Krejci L., Song B., Bussen W., Rothstein R., Mortensen U.H., and Sung P. Interaction with Rad51 is indispensable for recombination mediator function of Rad52. J. Biol. Chem. 277 (2002) 40132-40141
15. Park M.S., Ludwig D.L., Stigger E., and Lee S.H. Physical interaction between human RAD52 and RPA is required for homologous recombination in mammalian cells. J. Biol. Chem. 271 (1996) 18996-19000
16. Gorlich D., and Mattaj I.W. Nucleocytoplasmic transport. Science 271 (1996) 1513-1518
17. Boulikas T. Nuclear import of DNA repair proteins. Anticancer Res. 17 (1997) 843-863
18. Sherman F. F.G.H.J. Methods in Yeast Genetics (1986), Cold Spring Harbor Lab. Press
19. Thomas B.J., and Rothstein R. Elevated recombination rates in transcriptionally active DNA. Cell 56 (1989) 619-630
20. Fan H.Y., Cheng K.K., and Klein H.L. Mutations in the RNA polymerase II transcription machinery suppress the hyperrecombination mutant hpr1 delta of Saccharomyces cerevisiae. Genetics 142 (1996) 749-759
21. Zou H., and Rothstein R. Holliday junctions accumulate in replication mutants via a RecA homolog-independent mechanism. Cell 90 (1997) 87-96
22. Erdeniz N., Mortensen U.H., and Rothstein R. Cloning-free PCR-based allele replacement methods. Genome Res. 7 (1997) 1174-1183
23. Lisby M., Rothstein R., and Mortensen U.H. Rad52 forms DNA repair and recombination centers during S phase. Proc. Natl. Acad. Sci. U.S.A. 98 (2001) 8276-8282
24. Feng Q., During L., de Mayolo A.A., Lettier G., Lisby M., Erdeniz N., et al. Rad52 and Rad59 exhibit both overlapping and distinct functions. DNA Repair (Amst) 6 (2007) 27-37
25. Mortensen U.H., Erdeniz N., Feng Q., and Rothstein R. A molecular genetic dissection of the evolutionarily conserved N terminus of yeast Rad52. Genetics 161 (2002) 549-562
26. Kalderon D., Richardson W.D., Markham A.F., and Smith A.E. Sequence requirements for nuclear location of simian virus 40 large-T antigen. Nature 311 (1984) 33-38
27. Kalderon D., and Smith A.E. In vitro mutagenesis of a putative DNA binding domain of SV40 large-T. Virology 139 (1984) 109-137
28. Mikkelsen L., Sarrocco S., Lubeck M., and Jensen D.F. Expression of the red fluorescent protein DsRed-Express in filamentous ascomycete fungi. FEMS Microbiol. Lett. 223 (2003) 135-139
29. Prakash L., and Prakash S. Isolation and characterization of MMS-sensitive mutants of Saccharomyces cerevisiae. Genetics 86 (1977) 33-55
30. Lisby M., ntunez de M.A., Mortensen U.H., and Rothstein R. Cell cycle-regulated centers of DNA double-strand break repair. Cell Cycle 2 (2003) 479-483
31. Asleson E.N., Okagaki R.J., and Livingston D.M. A core activity associated with the N terminus of the yeast RAD52 protein is revealed by RAD51 overexpression suppression of C-terminal rad52 truncation alleles. Genetics 153 (1999) 681-692
32. Hicks G.R., and Raikhel N.V. Protein import into the nucleus: an integrated view. Annu. Rev. Cell Dev. Biol. 11 (1995) 155-188
33. Boulikas T. Nuclear localization signals (NLS). Crit Rev. Eukaryot. Gene Expr. 3 (1993) 193-227
34. Cokol M., Nair R., and Rost B. Finding nuclear localization signals. EMBO Rep. 1 (2000) 411-415
35. Lacasse E.C., and Lefebvre Y.A. Nuclear localization signals overlap DNA- or RNA-binding domains in nucleic acid-binding proteins. Nucleic Acids Res. 23 (1995) 1647-1656
36. Stasiak A.Z., Larquet E., Stasiak A., Muller S., Engel A., Van Dyck E., et al. The human Rad52 protein exists as a heptameric ring. Curr. Biol. 10 (2000) 337-340
37. Kummalue T., Lou J., and Friedman A.D. Multimerization via its myosin domain facilitates nuclear localization and inhibition of core binding factor (CBF) activities by the CBFbeta-smooth muscle myosin heavy chain myeloid leukemia oncoprotein. Mol. Cell Biol. 22 (2002) 8278-8291
38. Kagawa W., Kurumizaka H., Ishitani R., Fukai S., Nureki O., Shibata T., et al. Crystal structure of the homologous-pairing domain from the human Rad52 recombinase in the undecameric form. Mol. Cell 10 (2002) 359-371
39. Singleton M.R., Wentzell L.M., Liu Y., West S.C., and Wigley D.B. Structure of the single-strand annealing domain of human RAD52 protein. Proc. Natl. Acad. Sci. U.S.A. 99 (2002) 13492-13497
40. Shinohara A., and Ogawa T. Stimulation by Rad52 of yeast Rad51-mediated recombination. Nature 391 (1998) 404-407
41. Bai Y., and Symington L.S. A Rad52 homolog is required for RAD51-independent mitotic recombination in Saccharomyces cerevisiae. Genes Dev. 10 (1996) 2025-2037
42. Lisby M., Barlow J.H., Burgess R.C., and Rothstein R. Choreography of the DNA damage response: spatiotemporal relationships among checkpoint and repair proteins. Cell 118 (2004) 699-713