Visualisation of human Rad52 protein and its complexes with hRad51 and DNA

Journal of Molecular Biology

Volumn 284, Issue 4, 1998, Pages 1027-1038

  Van Dyck, Eric ^a   Hajibagheri, Nasser M A ^a   Stasiak, Andrzej ^b   West, Stephen C ^a  

^a IMPERIAL CANCER RESEARCH FUND   (United Kingdom)

^b UNIVERSITY OF LAUSANNE   (Switzerland)

Author keywords

DNA repair; Electron microscopy; Genetic recombination; Protein DNA interactions; Ring proteins

Indexed keywords

ANTIBODY; DNA BINDING PROTEIN; DOUBLE STRANDED DNA; NUCLEOPROTEIN; SINGLE STRANDED DNA;

ARTICLE; CONTROLLED STUDY; DNA PROBE; DNA PROTEIN COMPLEX; DNA REPAIR; GENETIC RECOMBINATION; HUMAN; PRIORITY JOURNAL; PROTEIN DNA INTERACTION; PROTEIN STRUCTURE;

ESCHERICHIA COLI;

EID: 0032509147     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.1998.2203     Document Type: Article

References (49)

1. Aboussekhra A., Chanet R., Adjiri A., Fabre F. Semidominant suppressors of srs2 helicase mutations of Saccharomyces cerevisiae map in theRAD51 gene, whose sequence predicts a protein with similarities to prokaryotic RecA proteins. Mol. Cell. Biol. 12:1992;3224-3234.
2. Adams D. E., Tsaneva I. R., West S. C. Dissociation of RecA filaments from duplex DNA by the RuvA and RuvB DNA repair proteins. Proc. Natl Acad. Sci. USA. 91:1994;9901-9905.
3. Barlow A. L., Benson F. E., West S. C., Hultén M. A. Distribution of Rad51 recombinase in human and mouse spermatocytes. EMBO J. 16:1997;5207-5215.
4. Basile G., Aker M., Mortimer R. K. Nucleotide-sequence and transcriptional regulation of the yeast recombinational repair gene RAD51. Mol. Cell. Biol. 12:1992;3235-3246.
5. Baumann P., West S. C. The human Rad51 protein: polarity of strand transfer and stimulation by hRP-A. EMBO J. 16:1997;5198-5206.
6. Baumann P., Benson F. E., West S. C. Human Rad51 protein promotes ATP-dependent homologous pairing and strand transfer reactions in vitro. Cell. 87:1996;757-766.
7. Baumann P., Benson F. E., Hajibagheri N., West S. C. Purification of human Rad51 protein by selective spermidine precipitation. Mutat. Res. DNA Repair. 384:1997;65-72.
8. Benson F. E., Stasiak A., West S. C. Purification and characterisation of the human Rad51 protein, an analogue of E. co li RecA. EMBO J. 13:1994;5764-5771.
9. Benson F. E., Baumann P., West S. C. Synergistic actions of Rad51 and Rad52 in genetic recombination and DNA repair. Nature. 391:1998;401-404.
10. Bezzubova O., Shinohara A., Mueller R. G., Ogawa H., Buerstedde J. M. A chicken RAD51 homologue is expressed at high levels in lymphoid and reproductive organs. Nucl. Acids Res. 21:1993;1577-1580.
11. Bishop D. K. RecA homologs Dmc1 and Rad51 interact to form multiple nuclear complexes prior to meiotic chromosome synapsis. Cell. 79:1994;1081-1092.
12. Game J. C. DNA double-strand breaks and the RAD50-RAD57 genes in Saccharomyces. Semin. Cancer Biol. 4:1993;73-83.
13. Gasior S. L., Wong A. K., Kora Y., Shinohara A., Bishop D. K. Rad52 associates with RPA and functions with Rad55 and Rad57 to assemble meiotic recombination complexes. Genes Dev. 12:1998;2208-2221.
14. Gupta R. C., Bazemore L. R., Golub E. I., Radding C. M. Activities of human recombination protein Rad51. Proc. Natl Acad. Sci. USA. 94:1997;463-468.
15. Haaf T., Golub E. I., Reddy G., Radding C. M., Ward D. C. Nuclear foci of mammalian Rad51 recombination protein in somatic cells after DNA damage and its localization in synaptonemal complexes. Proc. Natl Acad. Sci. USA. 92:1995;2298-2302.
16. Hall S. D., Kane M. F., Kolodner R. D. Identification and characterization of the Escherichia coli RecT protein, a protein encoded by therecE region that promotes renaturation of homologous single-stranded DNA. J. Bacteriol. 175:1993;277-287.
17. Hays S. L., Firmenich A. A., Berg P. Complex formation in yeast double-strand break repair: participation of Rad51, Rad52, Rad55, and Rad57 proteins. Proc. Natl Acad. Sci. USA. 92:1995;6925-6929.
18. Hingorani M. M., O'Donnell M. Toroidal proteins: running rings around DNA. Curr. Biol. 8:1998;R83-R86.
19. Ikeya T., Shinohara A., Sato S., Tabata S., Ogawa T. Localization of mouse Rad51 and Lim15 proteins on meiotic chromosomes at late stages of prophase 1. Genes Cells. 1:1996;379-389.
20. Kmiec E., Holloman W. K. β Protein of bacteriophage λ promotes renaturation of DNA. J. Biol. Chem. 256:1981;12636-12639.
21. Lim D. S., Hasty P. A mutation in mouse RAD51 results in an early embryonic lethal that is suppressed by a mutation in p53. Mol. Cell. Biol. 16:1996;7133-7143.
22. Milne G. T., Weaver D. T. Dominant-negative alleles of RAD52 reveal a DNA repair recombination complex including Rad51 and Rad52. Genes Dev. 7:1993;1755-1765.
23. Moens P. B., Chen D. J., Shen Z. Y., Kolas N., Tarsounas M., Heng H. H. Q., Spyropolos B. Rad51 immunocytology in rat and mouse spermatocytes and oocytes. Chromosoma. 106:1997;207-215.
24. Morita T., Yoshimura Y., Yamamoto A., Murata K., Mori M., Yamamoto H., Matsushiro A. A mouse homolog of the Escherichia coli recA and Saccharomyces cerevisiae RAD51 genes. Proc. Natl Acad. Sci. USA. 90:1993;6577-6580.
25. Mortensen U. H., Bendixen C., Sunjevaric I., Rothstein R. DNA strand annealing is promoted by the yeast Rad52 protein. Proc. Natl Acad. Sci. USA. 93:1996;10729-10734.
26. New J. H., Sugiyama T., Zaitseva E., Kowalczykowski S. C. Rad52 protein stimulates DNA strand exchange by Rad51 and replication protein-A. Nature. 391:1998;407-410.
27. Ogawa T., Yu X., Shinohara A., Egelman E. H. Similarity of the yeast Rad51 filament to the bacterial RecA filament. Science. 259:1993;1896-1899.
28. Petes T. D., Malone R. E., Symington L. S. Recombination in yeast. The Molecular and Cellular Biology of the Yeast Saccharomyces:Genome Dynamics, Protein Synthesis and Energetics. 1991;Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY. p. 407-521.
29. Plug A. W., Xu J. H., Reddy G., Golub E. I., Ashley T. Presynaptic association of Rad51 protein with selected sites in meiotic chromatin. Proc. Natl Acad. Sci. USA. 93:1996;5920-5924.
30. Rattray A. J., Symington L. S. Use of a chromosomal inverted repeat to demonstrate that the RAD51 and RAD52 genes ofSaccharomyces cerevisiae have different roles in mitotic recombination. Genetics. 138:1994;587-595.
31. Reddy G., Golub E. I., Radding C. M. Human Rad52 protein promotes single-strand DNA annealing followed by branch migration. Mutat. Res. 377:1997;53-59.
32. Sharan S. K., Morimatsu M., Albrecht U., Lim S. S., Regel E., Dinh C., Sands A., Eichele G., Hasty P., Bradley A. Embryonic lethality and radiation hypersensitivity mediated by Rad51 in mice lacking Brca2. Nature. 386:1997;804-810.
33. Shen Z., Peterson S. R., Comeaux J. C., Zastrow D., Moyzis R., Bradbury E. M., Chen D. J. Self-association of human Rad52 protein. Mutat. Res. 364:1996a;81-89.
34. Shen Z. Y., Cloud K. G., Chen D. J., Park M. S. Specific interactions between the human Rad51 and Rad52 proteins. J. Biol. Chem. 271:1996b;148-152.
35. Shinohara A., Ogawa T. Stimulation by Rad52 of yeast Rad51-mediated recombination. Nature. 391:1998;404-407.
36. Shinohara A., Ogawa H., Ogawa T. Rad51 protein involved in repair and recombination in Saccharomyces cerevisiae is a RecA-like protein. Cell. 69:1992;457-470.
37. Shinohara A., Ogawa H., Matsuda Y., Ushio N., Ikeo K., Ogawa T. Cloning of human, mouse and fission yeast recombination genes homologous to RAD51 and recA. Nature Genet. 4:1993;239-243.
38. Shinohara A., Shinohara M., Ohta T., Matsuda S., Ogawa T. Rad52 forms ring structures and cooperates with RPA in single-strand DNA annealing. Genes Cells. 3:1998;145-156.
39. Slot J. W., Geuze H. J., Gigengack S., Lienhard G. E., James D. E. Immunolocalization of the insulin regulatable glucose transporter in brown adipose tissue of the rat. J. Cell. Biol. 113:1991;123-135.
40. Sonoda E., Sasaki M. S., Buerstedde J. M., Bezzubova O., Shinohara A., Ogawa H., Takata M., Yamaguchi-Iwai Y., Takeda S. Rad51 deficient vertebrate cells accumulate chromosomal breaks prior to cell death. EMBO J. 17:1998;598-608.
41. Spiess E., Zimmerman H., Lunsdorf H. Negative staining of proteins and filaments. Sommerville J., Scheer U. Electron Microscopy in Molecular Biology. 1987;147-166 IRL Press, Oxford.
42. Stasiak A., DiCapua E. The helicity of DNA in complexes with RecA protein. Nature. 299:1982;185-186.
43. Stasiak A., Tsaneva I. R., West S. C., Benson C. J. B., Yu X., Egelman E. H. The Escherichia coli RuvB branch migration protein forms double hexameric rings around DNA. Proc. Natl Acad. Sci. USA. 91:1994;7618-7622.
44. Sugiyama T., New J. H., Kowalczykowski S. C. DNA annealing by Rad52 protein is stimulated by specific interaction with the complex of replication protein A and single-stranded DNA. Proc. Natl Acad. Sci. USA. 95:1998;6049-6054.
45. Sung P. Catalysis of ATP-dependent homologous DNA pairing and strand exchange by yeast Rad51 protein. Science. 265:1994;1241-1243.
46. Sung P. Function of yeast Rad52 protein as a mediator between replication protein-A and the Rad51 recombinase. J. Biol. Chem. 272:1997;28194-28197.
47. Sung P., Robberson D. L. DNA strand exchange mediated by a Rad51-ssDNA nucleoprotein filament with polarity opposite to that of RecA. Cell. 82:1995;453-461.
48. Thresher R. J., Makhov A. M., Hall S. D., Kolodner R., Griffith J. D. Electron microscopic visualization of RecT protein and its complexes with DNA. J. Mol. Biol. 254:1995;364-371.
49. Tsuzuki T., Fujii Y., Sakuma K., Tominaga Y., Nakao K., Sekiguchi M., Matsushiro A., Yoshimura Y., Morita T. Targeted disruption of the RAD51 gene leads to lethality in embryonic mice. Proc. Natl Acad. Sci. USA. 93:1996;6236-6240.