Saturation mutagenesis of Asn152 reveals a substrate selectivity switch in P99 cephalosporinase

Protein Science

Volumn 16, Issue 12, 2007, Pages 2636-2646

  Lefurgy, Scott T ^a,b   De Jong, René M ^a   Cornish, Virginia W ^a  

^a Och Spine at New York Presbyterian Hospitals   (United States)

^b ALBERT EINSTEIN COLLEGE OF MEDICINE   (United States)

Author keywords

lactamase; Enzymology; Kinetics; Microbial sensitivity tests; Saturation mutagenesis; Substrate selectivity

Indexed keywords

AMPICILLIN; ASPARAGINE; CEFALOTIN; CEFOTAXIME; CEFOXITIN; CEPHALOSPORINASE; CEPHALOSPORINASE P99; GLYCINE; MUTANT PROTEIN; NITROCEFIN; OXACILLIN; PENICILLIN G; SERINE; THREONINE; UNCLASSIFIED DRUG;

ANTIBIOTIC SENSITIVITY; ARTICLE; DRUG HYDROLYSIS; ENTEROBACTER CLOACAE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME SPECIFICITY; ESCHERICHIA COLI; HYDROLYSIS; IN VITRO STUDY; IN VIVO STUDY; MICHAELIS CONSTANT; MUTAGENESIS; PRIORITY JOURNAL;

ANTI-BACTERIAL AGENTS; BINDING SITES; CEPHALOSPORINASE; ESCHERICHIA COLI; HYDROGEN BONDING; KINETICS; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; MUTATION; PROTEIN BINDING; SUBSTRATE SPECIFICITY;

ENTEROBACTER CLOACAE; ESCHERICHIA COLI;

EID: 36448945773     PISSN: 09618368     EISSN: 1469896X     Source Type: Journal    
DOI: 10.1110/ps.073092407     Document Type: Article

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