X-ray Structures of the Signal Recognition Particle Receptor Reveal Targeting Cycle Intermediates

PLoS ONE

Volumn 2, Issue 7, 2007, Pages

  Reyes, Christopher L ^a,b   Rutenber, Earl ^a   Walter, Peter ^a   Stroud, Robert M ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b BIOGEN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; CELL RECEPTOR; FTSY PROTEIN, BACTERIA; GUANOSINE TRIPHOSPHATASE; GUANYLYLIMIDODIPHOSPHATE; NUCLEOTIDE; PROTEIN; RECEPTOR; SIGNAL PEPTIDE RECEPTOR; SIGNAL RECOGNITION PARTICLE;

ARTICLE; CELL CYCLE; CHEMICAL STRUCTURE; CHEMISTRY; DIMERIZATION; ENDOPLASMIC RETICULUM; ENZYME ACTIVE SITE; ENZYME SPECIFICITY; GENETICS; KINETICS; METABOLISM; PHYSIOLOGY; PROTEIN BINDING; PROTEIN SYNTHESIS; THERMUS;

BACTERIAL PROTEINS; CATALYTIC DOMAIN; CELL CYCLE; DIMERIZATION; ENDOPLASMIC RETICULUM; GTP PHOSPHOHYDROLASES; GUANYLYL IMIDODIPHOSPHATE; KINETICS; MODELS, MOLECULAR; NUCLEOTIDES; PROTEIN BINDING; PROTEIN BIOSYNTHESIS; PROTEINS; RECEPTORS, CYTOPLASMIC AND NUCLEAR; RECEPTORS, PEPTIDE; SIGNAL RECOGNITION PARTICLE; SUBSTRATE SPECIFICITY; THERMUS;

EID: 36148931107     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0000607     Document Type: Article

References (34)

1. Walter P, Johnson AE, (1994) Signal sequence recognition and protein targeting to the endoplasmic reticulum membrane. Annu Rev Cell Biol 10: 87-119.
2. Bourne HR, Sanders DA, McCormick F, (1991) The GTPase superfamily: conserved structure and molecular mechanism. Nature 349: 117-27.
3. Keenan RJ, Freymann DM, Stroud RM, Walter P, (2001) The Signal Recognition Particle. Annu Rev Biochem 70: 755-775.
4. Jagath JR, Rodnina MV, Wintermeyer W, (2000) Conformational changes in the bacterial SRP receptor FtsY upon binding of guanine nucleotides and SRP. Journal of Molecular Biology 295: 745-53.
5. Moser C, Mol O, Goody RS, Sinning I, (1997) The signal recognition particle receptor of Escherichia coli (FtsY) has a nucleotide exchange factor built into the GTPase domain. Proc Natl Acad Sci U S A 94: 11339-44.
6. Powers T, Walter P, (1995) Reciprocal Stimulation of GTP Hydrolysis by Two Directly Interacting GTPases. Science 269: 1422-1424.
7. Freymann DM, Keenan RJ, Stroud RM, Walter P, (1997) Structure of the conserved GTPase domain of the signal recognition particle. Nature 385: 361-4.
8. Montoya G, Svensson C, Luirink J, Sinning I, (1997) Expression, crystallization and preliminary X-ray diffraction study of FtsY, the docking protein of the signal recognition particle of E. coli. Proteins 28: 285-8.
9. Freymann DM, Keenan RJ, Stroud RM, Walter P, (1999) Functional changes in the structure of the SRP GTPase on binding GDP and Mg2+GDP. Nature Structural Biology 6: 793-801.
10. Padmanabhan S, Freymann DM, (2001) The conformation of bound gmppnp suggests a mechanism for gating the active site of the srp gtpase. Structure (Camb) 9: 859-67.
11. Gawronski-Salerno J, Coon VJ, Focia PJ, Freymann DM, (2006) X-ray structure of the T. Aquaticus Ftsy:GDP complex suggests functional roles for the C-terminal helix of the SRP GTPases. Proteins.
12. Montoya G, Svensson C, Luirink J, Sinning I, (1997) Crystal structure of the NG domain from the signal-recognition particle receptor FtsY. Nature 385: 365-8.
13. Gariani T, Samuelsson T, Sauer-Eriksson AE, (2006) Conformational variability of the GTPase domain of the signal recognition particle receptor FtsY. J Struct Biol 153: 85-96.
14. Joint Center for Structural Genomics(2007) Crystal structure of cell division protein ftsY (TM0570) from Thermotoga maritima at 1.60 Å resolution.
15. Peluso P, Shan SO, Nock S, Herschlag D, Walter P, (2001) Role of SRP RNA in the GTPase cycles of Ffh and FtsY. Biochemistry 40: 15224-15233.
16. Shan S, Walter P, (2003) Induced nucleotide specificity in a GTPase. Proceedings of the National Academy of Sciences of the United States of America 100: 4480-4485.
17. Egea PF, Shan SO, Napetschnig J, Savage DF, Walter P, et al.(2004) Substrate twinning activates the signal recognition particle and its receptor. Nature 427: 215-221.
18. Focia PJ, Shepotinovskaya IV, Seidler JA, Freymann DM, (2004) Heterodimeric GTPase core of the SRP targeting complex. Science 303: 373-377.
19. Gawronski-Salerno J, Freymann DM, (2006) Structure of the GMPPNP-stabilized NG domain complex of the SRP GTPases Ffh and FtsY. J Struct Biol.
20. Focia PJ, Gawronski-Salerno J, Coon JS, Freymann DM, (2006) Structure of a GDP:AlF4 complex of the SRP GTPases Ffh and FtsY, and identification of a peripheral nucleotide interaction site. J Mol Biol 360: 631-643.
21. Connolly T, Gilmore R, (1989) The signal recognition particle receptor mediates the GTP-dependent displacement of SRP from the signal sequence of the nascent polypeptide. Cell 57: 599-610.
22. Connolly T, Rapiejko PJ, Gilmore R, (1991) Requirement of GTP hydrolysis for dissociation of the signal recognition particle from its receptor. Science 252: 1171-3.
23. Shan SO, Stroud RM, Walter P, (2004) Mechanism of association and reciprocal activation of two GTPases. PLoS Biol 2: e320.
24. Shan SO, Walter P, (2003) Induced nucleotide specificity in a GTPase. Proc Natl Acad Sci U S A 100: 4480-4485.
25. de Leeuw E, Poland D, Mol O, Sinning I, ten Hagen-Jongman CM, et al.(1997) Membrane association of FtsY, the E. coli SRP receptor. FEBS Lett 416: 225-9.
26. de Leeuw E, te Kaat K, Moser C, Menestrina G, Demel R, et al.(2000) Anionic phospholipids are involved in membrane association of FtsY and stimulate its GTPase activity. Embo J 19: 531-41.
27. Zelazny A, Seluanov A, Cooper A, Bibi E, (1997) The NG domain of the prokaryotic signal recognition particle receptor, FtsY, is fully functional when fused to an unrelated integral membrane polypeptide. Proceedings of the National Academy of Sciences of the United States of America 94: 6025-9.
28. Shepotinovskaya IV, Freymann DM, (2002) Conformational change of the N-domain on formation of the complex between the GTPase domains of Thermus aquaticus Ffh and FtsY. Biochim Biophys Acta 1597: 107-114.
29. Sprang SR, (1997) G protein mechanisms: insights from structural analysis. Annu Rev Biochem 66: 639-78.
30. Zhang JT, (2000) Determinant of the extracellular location of the N-terminus of human multidrug-resistance-associated protein. Biochemical Journal 348 Pt 3: 597-606.
31. Ihara K, Muraguchi S, Kato M, Shimizu T, Shirakawa M, et al.(1998) Crystal structure of human RhoA in a dominantly active form complexed with a GTP analogue. J Biol Chem 273: 9656-66.
32. CCP4(1994) The CCP4 Suite: Programs for Protein Crystallography. Acta Crystallogr D 50: 760-763.
33. Otwinowski Z, Minor W, (1997) Processing of X-ray Diffraction Data Collected in Oscillation Mode. Methods in Enzymology 276: 307-326.
34. Brunger AT, Adams PD, Clore GM, DeLano WL, Gros P, et al.(1998) Crystallography & NMR system: A new software suite for macromolecular structure determination. Acta Crystallogr D Biol Crystallogr 54: 905-921.