Functional changes in the structure of the SRP GTPase on binding GDP and Mg2+GDP

Nature Structural Biology

Volumn 6, Issue 8, 1999, Pages 793-801

  Freymann, Douglas M ^a   Keenan, Robert J ^b   Stroud, Robert M ^b   Walter, Peter ^b,c  

^a NORTHWESTERN UNIVERSITY   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GUANOSINE DIPHOSPHATE; GUANOSINE TRIPHOSPHATASE; MAGNESIUM ION; SIGNAL RECOGNITION PARTICLE;

ARTICLE; CONFORMATIONAL TRANSITION; CRYSTAL STRUCTURE; CRYSTALLIZATION; METAL BINDING; MOLECULAR INTERACTION; MOLECULAR MODEL; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN PROTEIN INTERACTION; STRUCTURE ANALYSIS;

AMINO ACID SEQUENCE; CRYSTALLOGRAPHY, X-RAY; GTP PHOSPHOHYDROLASES; GUANINE; GUANOSINE DIPHOSPHATE; MAGNESIUM; MODELS, MOLECULAR; PROTEIN BINDING; PROTEIN CONFORMATION; STRUCTURE-ACTIVITY RELATIONSHIP;

EID: 0032813781     PISSN: 10728368     EISSN: None     Source Type: Journal    
DOI: 10.1038/11572     Document Type: Article

References (9)

1. Walter, P. & Johnson, A.E. Signal sequence recognition and protein targeting to the endoplasmic reticulum membrane. Annu. Rev. Cell Biol. 10, 87-119 (1994).
2. Miller, J.D., Bernstein, H.D. & Walter, P. Interaction of E. coli Ffh/4.5S ribonucleoprotein and FtsY mimics that of mammalian signal recognition particle and its receptor. Nature 367, 657-659 (1994).
3. Kusters, R. et al. The functioning of the SRP receptor FtsY in protein-targeting in E. coli is correlated with its ability to bind and hydrolyse GTP. FEBS Lett. 372, 253-258 (1995).
4. Powers, T. & Walter, P. Co-translational protein targeting catalyzed by the Escherichia coli signal recognition particle and its receptor. EMBO J. 16, 4880-4886 (1997).
5. Powers, T. & Walter, P. Reciprocal stimulation of GTP hydrolysis by two directly interacting GTPases. Science 269, 1422-1424 (1995).
6. Hauser, S., Bacher, G., Dobberstein, B. & Lütcke, H. A complex of the signal sequence binding protein and the SRP RNA promotes translocation of nascent proteins. EMBO J. 14, 5483-5493 (1995).
7. Rapiejko, P.J. & Gilmore, R. Signal sequence recognition and targeting of ribosomes to the endoplasmic reticulum by the signal recognition particle do not require GTP. Mol. Biol. Cell 5, 887-897 (1994).
8. Connolly, T. & Gillmore, R. The signal recognition particle receptor mediates the GTP-dependent displacement of SRP from the signal sequence of the nascent polypeptide. Cell 57, 599-610 (1989).
9. Zopf, D., Bernstein, H., Johnson, A.E. & Walter, P. The methionine-rich domain of the 54 kD protein subunit of the signal recognition particle contains an RNA binding site and can be crosslinked to a signal sequence. EMBO J. 9, 4511-4517 (1990).