Conformational changes in the bacterial SRP receptor FtsY upon binding of guanine nucleotides and SRP

Journal of Molecular Biology

Volumn 295, Issue 4, 2000, Pages 745-753

  Jagath, Junutula R ^a   Rodnina, Marina V ^a   Wintermeyer, Wolfgang ^a  

^a UNIVERSITY OF WITTEN HERDECKE   (Germany)

Author keywords

Fluorescence; GTPase; Protein targeting; Rapid kinetics; Signal recognition particle

Indexed keywords

GUANINE NUCLEOTIDE; GUANOSINE TRIPHOSPHATASE; GUANOSINE TRIPHOSPHATE; SIGNAL PEPTIDE; SIGNAL RECOGNITION PARTICLE;

ARTICLE; CELL MEMBRANE; CONFORMATIONAL TRANSITION; CROSS LINKING; ESCHERICHIA COLI; FLUORESCENCE; NONHUMAN; PRIORITY JOURNAL; PROTEIN INTERACTION; RECEPTOR BINDING; RIBOSOME; SEQUENCE HOMOLOGY; SITE DIRECTED MUTAGENESIS;

ESCHERICHIA COLI;

EID: 0034723143     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.1999.3427     Document Type: Article

References (31)

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3. Functional substitution of the signal recognition particle 54-kDa subunit by its Escherichia coli homolog
4. The E. coli SRP: Preferences of a targeting factor
5. Differential use of the signal recognition particle translocase targeting pathway for inner membrane protein assembly in Escherichia coli
6. Structure of the conserved GTPase domain of the signal recognition particle
7. Calculation of protein extinction coefficients from amino acid sequence data
8. Interaction of guanine nucleotides with the signal recognition particle from Escherichia coli
9. Crystal structure of the signal sequence binding subunit of the signal recognition particle
10. An improved PCR-mutagenesis strategy for two-site mutagenesis or sequence swapping between related genes
11. The functioning of the SRP receptor FtsY in protein-targeting in E. coli is correlated with its ability to bind and hydrolyse GTP
12. Formation of SRP-like particle induces a conformational change in E. coli 4. 5 S RNA
13. Signal-sequence recognition by an Escherichia coli ribonucleoprotein complex
14. An alternative protein targeting pathway in Escherichia coli: Studies on the role of FtsY
15. Interaction of E. coli Ffh/4.5 S ribonucleoprotein and FtsY mimics that of mammalian signal recognition particle and its receptor
16. Switching the model: A concerted mechanism for GTPases in protein targeting
17. Crystal structure of the NG domain from the signal recognition particle receptor FtsY
18. The signal recognition particle receptor of Escherichia coli (FtsY) has a nucleotide exchange factor built into the GTPase domain
19. Purines and pyrimidines
20. An E. coli ribonucleoprotein containing 4. 5 S RNA resembles mammalian signal recognition particle
21. Reciprocal stimulation of GTP hydrolysis by two directly interacting GTPases
22. Empty site forms of the SRP54 and SR alpha GTPases mediate targeting of ribosome-nascent chain complexes to the endoplasmic reticulum
23. Protein transport across the eukaryotic endoplasmic reticulum and bacterial inner membranes
24. E. coli 4. 5 S RNA is part of a ribonucleoprotein particle that has properties related to signal recognition particle
25. GTP consumption of elongation factor Tu during translation of heteropolymeric mRNAs
26. Homology of 54k protein of signal recognition particle, docking protein and two E. coli proteins with putative GTP-binding domains
27. Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes
28. The E. coli signal recognition particle is required for the insertion of a subset of inner membrane proteins
29. The Escherichia coli SRP and SecB targeting pathways converge at the translocon
30. The NG domain of the prokaryotic signal recognition particle receptor, FtsY, is fully functional when fused to an unrelated integral membrane polypeptide