Comparative Metabolic Flux Analysis of Lysine-Producing Corynebacterium glutamicum Cultured on Glucose or Fructose

Applied and Environmental Microbiology

Volumn 70, Issue 1, 2004, Pages 229-239

  Kiefer, Patrick ^a   Heinzle, Elmar ^a   Zelder, Oskar ^b   Wittmann, Christoph ^a  

^a SAARLAND UNIVERSITY   (Germany)

^b BASF SE   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

AMPLIFICATION; ENZYMES; FRUCTOSE; GAS CHROMATOGRAPHY; GLUCOSE; GLYCEROL; MASS SPECTROMETRY; METABOLISM; OPTIMIZATION;

METABOLIC FLUX;

BACTERIOLOGY;

CARBON; CARBON 13; DIHYDROXYACETONE; DIHYDROXYACETONE KINASE; DIHYDROXYACETONE PHOSPHATASE; FRUCTOSE; FRUCTOSE BISPHOSPHATASE; FRUCTOSE BISPHOSPHATE; GENE PRODUCT; GLUCOSE; GLYCEROL; LACTIC ACID; LYSINE; MALATE DEHYDROGENASE (DECARBOXYLATING); PHOSPHATASE; PHOSPHOENOLPYRUVATE SUGAR PHOSPHOTRANSFERASE; PHOSPHOTRANSFERASE; PYRUVATE DEHYDROGENASE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL METABOLISM; BACTERIUM CULTURE; BIOMASS; CITRIC ACID CYCLE; COMPARATIVE STUDY; CORYNEBACTERIUM GLUTAMICUM; ENZYME ACTIVITY; ENZYME INACTIVATION; ENZYME SPECIFICITY; GAS CHROMATOGRAPHY; GLUCOSE TRANSPORT; MASS SPECTROMETRY; METABOLIC INHIBITION; NONHUMAN;

CARBON ISOTOPES; CORYNEBACTERIUM; CULTURE MEDIA; FRUCTOSE; GAS CHROMATOGRAPHY-MASS SPECTROMETRY; GLUCOSE; LYSINE; SUBSTRATE SPECIFICITY;

ACTINOBACTERIA (CLASS); BACTERIA (MICROORGANISMS); CORYNEBACTERIUM; CORYNEBACTERIUM GLUTAMICUM; UNCULTURED ACTINOMYCETE;

EID: 0347761177     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.70.1.229-239.2004     Document Type: Article

References (22)

1. Cocaign, M., C. Monnet, and N. D. Lindley. 1993. Batch kinetics in Corynebacterium glutamicum during growth on various carbon substrates: use of substrate mixtures to localize metabolic bottlenecks. Appl. Microbiol. Biotechnol. 40:526-530.
2. Cocaign-Bousquet, M., and N. D. Lindley. 1995. Pyruvate overflow and carbon flux within the central metabolic pathways of Corynebacterium glutamicum during growth on lactate. Enz. Microbiol. Technol. 17:260-267.
3. de Graaf, A. A. 2000. Metabolic flux analysis of Corynebacterium glutamicum, p. 506-555. In: K. Schügerl and K. H. Bellgardt (ed.), Bioreaction engineering. Springer Verlag, Berlin, Germany.
4. Dominguez, H., and N. D. Lindley. 1996. Complete sucrose metabolism requires fructose phosphotransferase activity in Corynebacterium glutamicum to ensure phosphorylation of liberated fructose. Appl. Environ. Microbiol. 62:3878-3880.
5. Dominguez, H., C. Rollin, A. Guyonvarch, J. L. Guerquin-Kern, M. Cocaign-Bousquet, and N. D. Lindley. 1998. Carbon-flux distribution in the central metabolic pathways of Corynebacterium glutamicum during growth on fructose. Eur. J. Biochem. 254:96-102.
6. Ikeda, M. 2003. Amino acid production processes. Adv. Biochem. Eng. Biotechnol. 79:1-36.
7. Kiefer, P., E. Heinzle, and C. Wittmann. 2002. Influence of glucose, fructose and sucrose on kinetics and stoichiometry of lysine production by Corynebacterium glutamicum. J. Ind. Microbiol. Biotechnol. 28:338-343.
8. Malin, G. M., and G. I. Bourd. 1991. Phosphotransferase-dependent glucose transport in Corynebacterium glutamicum. J. Appl. Bacteriol. 71:517-523.
9. Marx, A., A. A. de Graaf, W. Wiechert, L. Eggeling, and H. Sahm. 1996. Determination of the fluxes in the central metabolism of Corynebacterium glutamicum by nuclear magnetic resonance spectroscopy combined with metabolite balancing. Biotechnol. Bioeng. 49:111-129.
10. Ohnishi, J., S. Mitsuhashi, M. Hayashi, S. Ando, H. Yokoi, K. Ochiai, and M. A. Ikeda. 2002. A novel methodology employing Corynebacterium glutamicum genome information to generate a new L-lysine producing mutant. Appl. Microbiol. Biotechnol. 58:217-223.
11. Pelechova, J., J. Smekal, V. Koura, J. Plachy, and V. Krumphanzl. 1980. Biosynthesis of L-lysine in Corynebacterium glutamicum on sucrose, ethanol and acetic acid. Folia Microbiol. 25:341-346.
12. Petersen, S., A. A. de Graaf, L. Eggeling, M. Möllney, W. Wiechert, and H. Sahm. 2000. In vivo quantification of parallel and bidirectional fluxes in the anaplerosis of Corynebacterium glutamicum. J. Biol. Chem. 75:35932-35941.
13. Petersson, G. 1974. Gas-chromatographic analysis of sugars and related hydroxyacids as acyclic oxime and ester trimethylsilyl derivates. Carbohydr. Res. 33:47-61.
14. Rubino, F. M. 1989. Silylaldonitrile derivates for the determination of sugars by gas chromatography-mass spectrometry. J. Chromatogr. 473:125-133.
15. Sahm, H., L. Eggeling, and A. A. de Graaf. 2000. Pathway analysis and metabolic engineering in Corynebacterium glutamicum. Biol. Chem. 381:899-910.
16. Shiio, I., S. Sugimoto, and K. Kawamura. 1990. Effects of carbon source sugars on the yield of amino acid production and sucrose metabolism in Brevibactetium flavum. Agric. Biol. Chem. 54:1513-1519.
17. Skrypal, I. G., and O. V. Iastrebova. 2002. Fructose-bisphosphatase of microorganisms. Mikrobiol. ZH. 64:82-94.
18. Wendisch, V. F., A. A. de Graaf, H. Sahm, and B. Eikmans. 2000. Quantitative determination of metabolic fluxes during coutilization of two carbon sources: comparative analyses with Corynebacterium glutamicum during growth on acetate and/or glucose. J. Bacteriol. 182:3088-3096.
19. Wittmann, C., and E. Heinzle. 2001. Application of MALDI-TOF MS to lysine-producing Corynebacterium glutamicum: a novel approach for metabolic flux analysis. Eur. J. Biochem. 268:2441-2455.
20. Wittmann, C., and E. Heinzle. 2002. Genealogy profiling through strain improvement by using metabolic network analysis: metabolic flux genealogy of several generations of lysine-producing corynebacteria. Appl. Environ. Microbiol. 68:5843-5859.
21. Wittmann, C., M. Hans, and E. Heinzle. 2002. In vivo analysis of intracellular amino acid labelings by GC/MS. Anal. Biochem. 307:379-382.
22. Wittmann, C., H. M. Kim, and E. Heinzle. Metabolic network analysis of lysine-producing Corynebacterium glutamicum at miniaturized scale. Biotechnol. Bioeng., in press.