Cloning of the thaumatin I cDNA and characterization of recombinant thaumatin I secreted by Pichia pastoris

Biotechnology Progress

Volumn 23, Issue 5, 2007, Pages 1023-1030

  Ide, Nobuyuki ^a   Kaneko, Ryosuke ^a   Wada, Ritsuko ^a   Mehta, Alka ^a   Tamaki, Shinobu ^a   Tsuruta, Tomoko ^a   Fujita, Yuki ^a   Masuda, Tetsuya ^a   Kitabatake, Naofumi ^a  

^a KYOTO UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

SWEET-TASTING PROTEIN; THAUMATIN; THAUMATOCOCCUS DANIELLII BENTH;

AMINO ACIDS; BIOTECHNOLOGY; CLONING; DNA; FRUITS;

PROTEINS;

COMPLEMENTARY DNA; RECOMBINANT PROTEIN; THAUMATIN PROTEIN, PLANT; UNCLASSIFIED DRUG; VEGETABLE PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; CHEMISTRY; GENETIC TRANSFECTION; GENETICS; METABOLISM; METHODOLOGY; MOLECULAR CLONING; MOLECULAR GENETICS; PICHIA;

AMINO ACID SEQUENCE; CLONING, MOLECULAR; DNA, COMPLEMENTARY; MOLECULAR SEQUENCE DATA; PICHIA; PLANT PROTEINS; RECOMBINANT PROTEINS; TRANSFECTION;

PICHIA PASTORIS; THAUMATOCOCCUS DANIELLII;

EID: 35348915322     PISSN: 87567938     EISSN: None     Source Type: Journal    
DOI: 10.1021/bp070072v     Document Type: Article

References (47)

1. van der Wel, H.; Loeve, K. Isolation and characterization of thaumatin I and II, the sweet-tasting proteins from Thaumatococcus daniellii Benth. Eur. J. Biochem. 1972, 31, 221-225.
2. Iyengar, R. B.; Smith, P.; van der Ouderaa, F.; van der Wel, H.; van Brouwershaven, J.; Ravestein, P.; Richters, G.; van Wassenaar, P. D. The complete amino-acid sequence of the sweet protein thaumatin I. Eur. J. Biochem. 1979, 96, 193-204.
3. Higginbotham, J. D.; Hough, C. A. M. Useful taste properties of amino acids and proteins. In Sensory Properties of Foods; Birch, G. C., Brennan, J. G., Perker, K. J., Eds.; Applied Sciences Publisher Ltd.: London, 1977; pp 129-149.
4. Mackenzie, A.; Pridham, J. B.; Saunders, N. A. Changes in the sweet proteins (thaumatins) in Thaumatococcus daniellii fruits during development. Photochemistry 1985, 24, 2503-2506.
5. Edens, L.; Heslinga, L.; Klok, R.; Ledeboer, A. M.; Maat, J.; Toonen, M. Y.; Visser, C.; Verrips, C. T. Cloning of cDNA encoding the sweet-tasting plant protein thaumatin and its expression in Escherichia coli. Gene 1982, 18, 1-12.
6. Masuda, T.; Tamaki, S.; Kaneko, R.; Wada, R.; Fujita, Y.; Mehta, A.; Kitabatake, N. Cloning, expression, and characterization of recombinant sweet-protein thaumatin II using the methylotrophic yeast Pichia pastoris. Biotechnol. Bioeng. 2004, 85, 761-769.
7. Lee, J. H.; Weickmann, J. L.; Koduri, R. K.; Ghosh, D. P.; Saito, K.; Blair, L. C.; Date, T.; Lai, J. S.; Hollenberg, S. M.; Kendall, R. L. Expression of synthetic thaumatin genes in yeast. Biochemistry 1988, 27, 5101-5107.
8. Faus, I.; Patiño, C.; del Río, J. L.; del Moral, C.; Barroso, H. S.; Rubio, V. Expression of a synthetic gene encoding the sweet-tasting protein thaumatin in Escherichia coli. Biochem. Biophys. Res. Commun. 1996, 229, 121-127.
9. Daniell, S.; Mellits, K. H.; Faus, I.; Connerton, I. Refolding the sweet-tasting protein thaumatin II from insoluble inclusion bodies synthesized in Escherichia coli. Food Chem. 2000, 71, 105-110.
10. Edens, L.; van der Wel, H. Microbial synthesis of the sweet-tasting plant protein thaumatin. Trends Biotechnol. 1985, 3, 61-64.
11. Illingworth, C.; Larson, G.; Hellekant, G. Secretion of the sweet-tasting plant protein thaumatin by Bacillus subtilis. Biotechnol. Lett. 1988, 10, 587-592.
12. Illingworth, C.; Larson, G.; Hellekant, G. Secretion of the sweet-tasting plant protein thaumatin by Streptomyces lividans. J. Ind. Microbiol. 1989, 4, 37-42.
13. Edens, L.; Bom, L.; Ledeboer, A. M.; Maat, J.; Toonen, M. Y.; Visser, C.; Verrips, C. T. Synthesis and processing of the plant protein thaumatin in yeast. Cell 1984, 37, 629-633.
14. Hahm, Y. T.; Batt, C. A. Expression and secretion of thaumatin from Aspergillus oryzae. Agric. Biol. Chem. 1990, 54, 2513-2520.
15. Faus, I.; Patiño, C.; del Río, J. L.; del Moral, C.; Barroso, H. S.; Bladé, J.; Rubio, V. Expression of a synthetic gene encoding the sweet-tasting protein thaumatin in the filamentous fungus Penicillium roquefortii. Biotechnol. Lett. 1997, 19, 1185-1191.
16. Faus, I. Recent developments in the characterization and biotechnological production of sweet-tasting proteins. Appl. Microbiol. Biotechnol. 2000, 53, 145-151.
17. Masuda, T.; Kitabatake, N. Developments in biotechnological production of sweet proteins. J. Biosci. Bioeng. 2006, 102, 375-389.
18. Weickmann, J. L.; Blair, L. C.; Wilcox, G. L. High level expression of thaumatin in Saccharomyces cerevisiae. In Thaumatin; Witty, M., Higginbotham, J. D., Eds.; CRC Press: Boca Raton, FL, 1994; pp 151-169.
19. Faus, I.; del Moral, C.; Adroer, N.; del Río, J. L.; Patiño, C.; Sisniega, H.; Casas, C.; Bladé, J.; Rubio, V. Secretion of the sweet-tasting protein thaumatin by recombinant strains of Aspergillus niger var. awamori. Appl. Microbiol. Biotechnol. 1998, 49, 393-398.
20. Moralejo, F. J.; Cardoza, R. E.; Gutierrez, S.; Martin, J. F. Thaumatin production in Aspergillus awamori by use of expression cassettes with strong fungal promoters and high gene dosage. Appl. Environ. Microbiol. 1999, 65, 1168-1174.
21. Moralejo, F. J.; Cardoza, R. E.; Gutiérrez, S.; Sisniega, H.; Faus, I.; Martin, J. F. Overexpression and lack of degradation of thaumatin in an aspergillopepsin A-defective mutant of Aspergillus awamori containing an insertion in the pepA gene. Appl. Microbiol. Biotechnol. 2000, 54, 772-777.
22. Lombraña, M.; Moralejo, F. J.; Pinto, R.; Martin, J. F. Modulation of Aspergillus awamori thaumatin secretion by modification of bipA gene expression. Appl. Environ. Microbiol. 2004, 70, 5145-5152.
23. Cereghino, J. L.; Cregg, J. M. Heterologous protein expression in the methylotrophic yeast Pichia pastoris. FEMS Microbiol. 2000, 1, 45-66.
24. Cregg, J. M.; Cereghino, J. L.; Shi, J.; Higgins, D. R. Recombinant protein expression in Pichia pastoris. Mol. Biotechnol. 2000, 16, 23-52.
25. Cereghino, G. P.; Cereghino, J. L.; Ilgen, C.; Cregg, J. M. Production of recombinant proteins in fermenter cultures of the yeast Pichia pastoris. Curr. Opin. Biotechnol. 2002, 13, 329-332.
26. Schilling, B. M.; Goodrick, J. C.; Wan, N. C. Scale-up of a high cell density continuous culture with Pichia pastoris X-33 for the constitutive expression of rh-chitinase. Biotechnol. Prog. 2001, 17, 629-633.
27. Hellwig, S.; Emde, F.; Raven, N. P. G.; Henke, M.; van der Logt, P.; Fischer, R. Analysis of single-chain antibody production in Pichia pastoris using on-line methanol control in fed-batch and mixed-feed fermentations. Biotechnol. Bioeng. 2001, 74, 344-352.
28. Goodrick, J. C.; Xu, M.; Finnegan, R.; Schilling, B. M.; Schiavi, S.; Hoppe, H. Wan, N. C. High-level expression and stabilization of recombinant human chitinase produced in a continuous constitutive Pichia pastoris expression system. Biotechnol. Bioeng. 2001, 74, 492-497.
29. Ogata, C. M.; Gordon, P. F.; de Vos, A. M.; Kim, S. H. Crystal structure of a sweet tasting protein thaumatin I, at 1.65 Å resolution. J. Mol. Biol. 1992, 228, 893-908.
30. Ko, T. P.; Day, J.; Greenwood, A.; McPherson, A. Structures of three crystal forms of the sweet protein thaumatin. Acta Crystallogr. D 1994, 50, 813-825.
31. van der Wel, H.; Bel, W. J. Effect of acetylation and methylation on the sweetness intensity of thaumatin I. Chem. Senses 1976, 2, 211-218.
32. Kaneko, R.; Kitabatake, N. Structure-sweetness relationship in thaumatin: Importance of lysine residues. Chem. Senses 2001, 26, 167-177.
33. Masuda, T.; Ueno, Y.; Kitabatake, N. High yield secretion of the sweet-tasting protein lysozyme from the yeast Pichia pastoris. Protein Expression Purif 2005, 39, 35-42.
34. Laemmli, U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970, 227, 680-685.
35. Reisfeld, R. A.; Lewis, U. J.; Williams, D. E. Disk electrophoresis of basic proteins and peptides on Polyacrylamide gels. Nature 1962, 195, 281-283.
36. Masuda, T.; Ide, N.; Kitabatake, N. Effects of chemical modification of lysine residues on the sweetness of lysozyme. Chem. Senses 2005, 30, 253-264.
37. Sano, H.; Egashira, T.; Kinekawa, Y.; Kitabatake, N. Astringency of bovine milk whey protein. J. Dairy Sci. 2005, 88, 2312-2317.
38. Rockwell, N. C.; Krysan, D. J.; Komiyama, T.; Fuller, R. S. Precursor processing by kex2/furin proteases. Chem. Rev. 2002, 102, 4525-4548.
39. Etheridge, K. The sales and marketing of talin. In Thaumatin; Witty, M., Higginbotham, J. D., Eds.; CRC Press: Boca Raton, FL, 1994; pp 47-59.
40. Kim, S. H.; Weickmann, J. L. Crystal structure of thaumatin I and its correlation to biochemical and mutational studies. In Thaumatin; Witty, M., Higginbotham, J. D., Eds.; CRC Press: Boca Raton, FL, 1994; pp 135-149.
41. Morris, J. A.; Cagan, R. H. Purification of monellin, the sweet principle of Dioscoreopyllum cumminsii. Biochem. Biophys. Acta 1972, 261, 114-122.
42. van der Wel, H. Isolation and characterization of the sweet principle for Dioscoreopyllum cumminsii (Stapf) Diels. FEBS Lett. 1972, 21, 88-90.
43. Liu, X.; Hu, Z.; Maeda, S.; Aiuchi, T.; Nakaya, K.; Kurihara, Y. Purification, complete amino acid sequence and structure characterization of the heat stable sweet protein, mabinlin II. Eur. J. Biochem. 1993, 211, 281-287.
44. Yamashita, H.; Theeraship, A.; Nakaya, T.; Nakamura, Y.; Kurihara, Y. Purification and complete amino acid sequence of a new type of sweet protein with taste-modifying activity, curculin. J. Biol. Chem. 1990, 265, 15770-15775.
45. Ming, D.; Hellekant, G. Brazzein, a new high-potency thermostable sweet protein from Pentadiplandra brazzeana B. FEBS Lett. 1994, 355, 106-108.
46. Masuda, T.; Ueno, Y.; Kitabatake, N. Sweetness and enzymatic activity of lysozyme. J. Agric. Food Chem. 2001, 49, 4937-4941.
47. Masuda, T.; Ide, N.; Kitabatake, N. Structure-sweetness relationship in egg white lysozyme: Role of lysine and arginine residues on the elicitation of the lysozyme sweetness. Chem. Senses 2005, 30, 667-681.