Generation and application of a fluorescein-specific single chain antibody

Biochimie

Volumn 89, Issue 11, 2007, Pages 1304-1311

  Schenk, Jörg A ^a   Sellrie, Frank ^a   Böttger, Volker ^b   Menning, Astrid ^a   Stöcklein, Walter F M ^a   Micheel, Burkhard ^a  

^a UNIVERSITY OF POTSDAM   (Germany)

^b Wilex Biotechnology GmbH   (Germany)

Author keywords

FITC; hCG; HEK293; Immunoassay; Periplasmic expression; Phage display; Single chain antibody

Indexed keywords

CHORIONIC GONADOTROPIN; FLUORESCEIN; HORSERADISH PEROXIDASE; MONOCLONAL ANTIBODY; MONOCLONAL ANTIBODY B13 DE1; RECOMBINANT ANTIBODY; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY; UNCLASSIFIED DRUG;

ARTICLE; BACTERIOPHAGE; BACTERIUM CULTURE; CELL STRAIN HEK293; COMPARATIVE STUDY; CONTROLLED STUDY; EMBRYO; ESCHERICHIA COLI; EUKARYOTIC CELL; EXPERIMENTAL MODEL; HEAVY CHAIN; HUMAN; HUMAN CELL; HYBRIDOMA; IMMUNOASSAY; LIGHT CHAIN; PHAGE DISPLAY; SUPERNATANT; SURFACE PLASMON RESONANCE; URINALYSIS;

AMINO ACID SEQUENCE; ANIMALS; ANTIBODIES, MONOCLONAL; ANTIBODY SPECIFICITY; CLONING, MOLECULAR; ESCHERICHIA COLI; FLUORESCEIN; FLUORESCEIN-5-ISOTHIOCYANATE; GENES, IMMUNOGLOBULIN HEAVY CHAIN; GENES, IMMUNOGLOBULIN LIGHT CHAIN; HUMANS; IMMUNOENZYME TECHNIQUES; IMMUNOGLOBULIN FRAGMENTS; IMMUNOGLOBULIN VARIABLE REGION; MICE; MOLECULAR SEQUENCE DATA; RECOMBINANT FUSION PROTEINS;

ARMORACIA RUSTICANA; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; EUKARYOTA; MURINAE;

EID: 35248844437     PISSN: 03009084     EISSN: 61831638     Source Type: Journal    
DOI: 10.1016/j.biochi.2007.06.008     Document Type: Article

References (31)

1. Harmer I.J., and Samuel D. The FITC-anti-FITC system is a sensitive alternative to biotin-streptavidin in ELISA. J. Immunol. Methods 122 (1989) 115-121
2. Micheel B., Jantscheff P., Bottger V., Scharte G., Kaiser G., Stolley P., and Karawajew L. The production and radioimmunoassay application of monoclonal antibodies to fluorescein isothiocyanate (FITC). J. Immunol. Methods 111 (1988) 89-94
3. Wilchek M., Bayer E.A., and Livnah O. Essentials of biorecognition: the (strept)avidin-biotin system as a model for protein-protein and protein-ligand interaction. Immunol. Lett. 103 (2006) 27-32
4. Balogh P., Szekeres G., and Nemeth P. Hapten-mediated identification of cell membrane antigens using an anti-FITC monoclonal antibody. J. Immunol. Methods 169 (1994) 35-40
5. van Dam G.J., Bogitsh B.J., Fransen J.A., Kornelis D., van Zeyl R.J., and Deelder A.M. Application of the FITC-anti-FITC-gold system to ultrastructural localization of antigens. J. Histochem. Cytochem. 39 (1991) 1725-1728
6. Samoszuk M.K., Walter J., and Mechetner E. Improved immunohistochemical method for detecting hypoxia gradients in mouse tissues and tumors. J. Histochem. Cytochem. 52 (2004) 837-839
7. Andris-Widhopf J., Rader C., Steinberger P., Fuller R., and Barbas III C.F. Methods for the generation of chicken monoclonal antibody fragments by phage display. J. Immunol. Methods 242 (2000) 159-181
8. Bedzyk W.D., Weidner K.M., Denzin L.K., Johnson L.S., Hardman K.D., Pantoliano M.W., Asel E.D., and Voss Jr. E.W. Immunological and structural characterization of a high affinity anti-fluorescein single-chain antibody. J. Biol. Chem. 265 (1990) 18615-18620
9. Vaughan T.J., Williams A.J., Pritchard K., Osbourn J.K., Pope A.R., Earnshaw J.C., McCafferty J., Hodits R.A., Wilton J., and Johnson K.S. Human antibodies with sub-nanomolar affinities isolated from a large non-immunized phage display library. Nat. Biotechnol. 14 (1996) 309-314
10. Böttger V., Peters L.E., and Micheel B. Identification of peptide mimotopes for the fluorescein hapten binding of monoclonal antibody B13-DE1. J. Mol. Recognit. 12 (1999) 191-197
11. Sellrie F., Schenk J.A., Behrsing O., Böttger V., and Micheel B. A competitive immunoassay to detect a hapten using an enzyme-labelled peptide mimotope as tracer. J. Immunol. Methods 261 (2002) 141-144
12. Karawajew L., Behrsing O., Kaiser G., and Micheel B. Production and ELISA application of bispecific monoclonal antibodies against fluorescein isothiocyanate (FITC) and horseradish peroxidase (HRP). J. Immunol. Methods 111 (1988) 95-99
13. McCafferty J., Fitzgerald K.J., Earnshaw J., Chiswell D.J., Link J., Smith R., and Kenten J. Selection and rapid purification of murine antibody fragments that bind a transition-state analog by phage display. Appl. Biochem. Biotechnol. 47 (1994) 157-173
14. Stöcklein W.F.M., Warsinke A., Micheel B., Kempter G., Höhne W., and Scheller F.W. Diphenylurea hapten sensing with a monoclonal antibody and its Fab fragment: kinetic and thermodynamic investigations. Anal. Chim. Acta 362 (1998) 101-111
15. Stöcklein W.F., Behrsing O., Scharte G., Micheel B., Benkert A., Schössler W., Warsinke A., and Scheller F.W. Enzyme kinetic assays with surface plasmon resonance (BIAcore) based on competition between enzyme and creatinine antibody. Biosens. Bioelectron. 15 (2000) 377-382
16. Böttger V., Micheel B., Scharte G., Kaiser G., Wolf G., and Schmechta H. Monoclonal antibodies to human chorionic gonadotropin (hCG) and their use in two-site binding enzyme immunoassays. Hybridoma 12 (1993) 81-91
17. Kabat E.A., Wu T.T., Reid-Miller M., Perry H.M., Gottesmann K.S., and Foeller C. Sequences of Proteins of Immunological Interest. US Department of Health and Human Services, Public Health Services. fifth ed. (1991), National Institute of Health, Bethesda, MD, USA
18. Martin A.C.R. Accessing the Kabat antibody sequence database by computer PROTEINS: structure, function. Genetics 25 (1996) 130-133
19. Altschul S.F., Gish W., Miller W., Myers E.W., and Lipman D.J. Basic local alignment search tool. J. Mol. Biol. 215 (1990) 403-410
20. Bergholz A., Heymann S., Schenk J.A., and Freytag J.C. Biological sequences integrated: a relational database approach. Acta Biotheor. 49 (2001) 145-159
21. Berman H.M., Westbrook J., Feng Z., Gilliland G., Bhat T.N., Weissig H., Shindyalov I.N., and Bourne P.E. The Protein Data Bank. Nucleic Acids Res. 28 (2000) 235-242
22. Boeckmann B., Bairoch A., Apweiler R., Blatter M.C., Estreicher A., Gasteiger E., Martin M.J., Michoud K., O'Donovan C., Phan I., Pilbout S., and Schneider M. The SWISS-PROT protein knowledgebase and its supplement TrEMBL in 2003. Nucleic Acids Res. 31 (2003) 365-370
23. Zhou T., Hamer D.H., Hendrickson W.A., Sattentau Q.J., and Kwong P.D. Interfacial metal and antibody recognition. Proc. Natl. Acad. Sci. USA 102 (2005) 14575-14580
24. Saxena A.K., Singh K., Long C.A., and Garboczi D.N. Preparation, crystallization and preliminary X-ray analysis of a complex between the Plasmodium vivax sexual stage 25 kDa protein Pvs25 and a malaria transmission-blocking antibody Fab fragment. Acta Crystallogr. D Biol. Crystallogr. 60 (2004) 2054-2057
25. Ho M., Nagata S., and Pastan I. Isolation of anti-CD22 Fv with high affinity by Fv display on human cells. Proc. Natl. Acad. Sci. USA 103 (2006) 9637-9642
26. Wang X.X., and Shusta E.V. The use of scFv-displaying yeast in mammalian cell surface selections. J. Immunol. Methods 304 (2005) 30-42
27. Krebber C., Spada S., Desplancq D., Krebber A., Ge L., and Pluckthun A. Selectively-infective phage (SIP): a mechanistic dissection of a novel in vivo selection for protein-ligand interactions. J. Mol. Biol. 268 (1997) 607-618
28. Killeen G.F., Foy B.D., Shahabuddin M., Roake W., Williams A., Vaughan T.J., and Beier J.C. Tagging bloodmeals with phagemids allows feeding of multiple-sample arrays to single cages of mosquitoes (Diptera: Culicidae) and the recovery of single recombinant antibody fragment genes from individual insects. J. Med. Entomol. 37 (2000) 528-533
29. Soderlind E., Strandberg L., Jirholt P., Kobayashi N., Alexeiva V., Aberg A.M., Nilsson A., Jansson B., Ohlin M., Wingren C., Danielsson L., Carlsson R., and Borrebaeck C.A. Recombining germline-derived CDR sequences for creating diverse single-framework antibody libraries. Nat. Biotechnol. 18 (2000) 852-856
30. Jorgensen T.O., Solem S.T., Espelid S., Warr G.W., Brandsdal B.O., and Smalas A. The antibody site in Atlantic salmon; phage display and modeling of scFv with anti-hapten binding ability. Dev. Comp. Immunol. 26 (2002) 201-206
31. Sellrie F., Warsinke A., and Micheel B. Homogeneous indirect fluoresfcence quenching immunoassay for the determination of low molecular weight substances. Anal. Bioanal. Chem. 386 (2006) 206-210