Alanine substitutions in calmodulin-binding peptides result in unexpected affinity enhancement

Journal of Molecular Biology

Volumn 258, Issue 1, 1996, Pages 6-13

  Montigiani, Silvia ^a   Neri, Giovanni ^a   Neri, Paolo ^a   Neri, Dario ^b  

^a UNIVERSITY OF SIENA   (Italy)

^b MEDICAL RESEARCH COUNCIL   (United Kingdom)

Author keywords

Alanine scanning; Calmodulin; Calmodulin binding peptides; Kinetic association constants; Kinetic dissociation constants

Indexed keywords

CALCIUM BINDING PROTEIN; CALMODULIN; CALMODULIN BINDING PROTEIN; MYOSIN LIGHT CHAIN KINASE;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ARTICLE; BINDING AFFINITY; BINDING KINETICS; CHEMICAL REACTION KINETICS; CONTROLLED STUDY; DISSOCIATION CONSTANT; ISOMERISM; LUMINESCENCE; PRIORITY JOURNAL; PROTEIN STRUCTURE; SKELETAL MUSCLE;

EID: 0030002422     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.1996.0229     Document Type: Editorial

References (29)

1. Afshar, M., Caves, L. S. D., Guimard, L., Hubbard, R. E., Calas, B., Grassy, G. & Haieck, J. (1994). Investigating the high affinity and low sequence specificity of calmodulin binding to its targets. J. Mol. Biol. 244, 554-571.
2. Bayer, E. A. & Wilchek, M. (1990). Protein biotinylation. Methods Enzymol. 184, 138-160.
3. 3H-melatonin to calmodulin. Life Sci. 53, 201-207.
4. Berndt, K. D., Beunink, J., Schroder, W. & Wüthrich, K. (1993). Designed replacement of an internal hydration water molecule in BPTI: structural and functional implications of a glycine-to-serine mutation. Biochemistry, 32, 4564-4570.
5. Bigler, T. L., Lu, W., Park, S. J., Tashiro, M., Wieczorek, M., Wunn, R. & Laskowski, M. Jr. (1993). Binding of amino acid side-chains to preformed cavities: interaction of serine proteinases with turkey ovomucoid third domains with coded and noncoded P1 residues. Protein Sci. 2, 786-799.
6. Blaber, M., Baase, W. A., Gassner, N. & Matthews, B. W. (1995). Alanine scanning mutagenesis of the α-helix 115-123 of phage T4 lysozyme: effects on structure, stability and the binding of solvent. J. Mol. Biol. 246, 317-330.
7. Clackson, T. & Wells, J. A. (1995). A hot-spot of binding energy in a hormone-receptor interface. Science, 267, 383-386.
8. Erhardt, M. R., Urbauer, J. L. & Wand, A. J. (1995). The energetics and dynamics of molecular recognition by calmodulin. Biochemistry, 34, 2731-2738.
9. Fersht, A. R., Wilkinson, A. J., Carter, P. & Winter, G. (1985). Fine structure-activity analysis of mutations at positions 51 of tyrosyl-tRNA synthetase. Biochemistry, 24, 5858-5861.
10. Fried, M. & Crothers, D. M. (1981). Equilibria and kinetics of lac represser-operator interactions by polyacrylamide gel electrophoresis. Nucl. Acids Res. 9, 6505-6525.
11. Garner, M. M. & Revzin, A. (1981). A gel electrophoresis method for quantifying the binding of proteins to specific DNA regions: applications to components of the Escherichia coli lactose operon regulatory system. Nucl. Acids Res. 9, 3047-3060.
12. Horovitz, A., Matthews, J. M. & Fersht, A. (1992). α-Helix stability in proteins. II. Factors that influence stability at internal position. J. Mol. Biol. 227, 560-568.
13. Ikura, M., Clore, G. M., Gronenborn, A. M., Zhu, G., Klee, C. B. & Bax, A. (1992). Solution structure of a calmodulin-target peptide complex by multidimensional NMR. Science, 256, 632-638.
14. Jönsson, U., Fägerstam, L., Ivarsson, B., Johnsson, B., Karlsson, R., Lundh, K., Löfås, S., Persson, B., Roos, H., Rönnberg, I., Sjölander, S., Stenberg, E., Ståhlberg, Urbaniczky C., Östlin, H. & Malmqvist, M. (1991). Real-time biospecific interaction analysis using surface plasmon resonance and a sensor chip technology BioTechniques, 11, 620-627.
15. Lowman, H. B. & Wells, J. A. (1993). Affinity maturation of human growth hormone by monovalent phage display. J. Mol. Biol. 234, 564-578.
16. Lukas, T., Haiech, J., Lau, W., Craig, T. A., Zimmer, W. E., Shattuck, R. L., Shoemaker, M. O. & Watterson, D. M. (1988). Calmodulin and calmodulin-regulated protein kinases as transducers of intracellular calcium signals. Cold Spring Harbor Symp. Quant. Biol. 53, 185-193.
17. Meador, W., Means, A. & Quiocho, F. (1992). Target enzyme recognition by calmodulin: 2.4 Å structure of a calmodulin-peptide complex. Science, 257, 1251-1257.
18. Meador, W., Means, A. & Quiocho, F. (1993). Modulation of calmodulin plasticity in molecular recognition on the basis of X-ray structures. Science, 262, 1718-1721.
19. Neri, D., de Lalla, C., Petrul, H., Neri, P. & Winter, G. (1995). Calmodulin as a versatile tag for antibody fragments. Bio/Technology, 13, 373-377.
20. Neri, D., Prospero, T., Petrul, H., Winter, G., Browne, M. & Vanderpant, L. (1996). A multi-purpose high sensitivity luminescence analyser (LUANA): use in gel-electrophoresis. BioTechniques, in the press.
21. O'Neil, K. & DeGrado, W (1989) The interaction of calmodulin with fluorescent and photoreactive model peptides: evidence for short interdomain separation. Proteins: Struct. Funct. Genet. 6, 284-293.
22. Paganelli, G., Magnani, P., Zito, F., Villa, E., Sudati, F., Lopalco, L., Rossetti, C., Malcovati, M., Chiolerio, F., Seccamani, E., Siccardi, A. G. & Fazio, F. (1991 Three-step monoclonal antibody tumour targeting in carcinoembryonic antigen-positive patients. Cancer Res. 51, 5960-5966.
23. Quadroni, M., James, P. & Carafoli, E. (1994). Isolation of phosphorylated calmodulin from rat liver and identification of the in vivo phosphorylation sites. J. Biol. Chem. 269, 16116-16122.
24. Schreiber, G., Buckle, A. M. & Fersht, A. (1994). Stability and function: two constraints in the evolution of barstar and other proteins. Structure, 2, 945-951.
25. Scott, J. K. (1992). Discovering peptide ligands using epitope libraries. Trends Biochem. Sci. 17, 241-245.
26. Stofko-Hahn, R. E., Carr, D. W. & Scott, J. D. (1992). A single step purification for recombinant proteins. FEBS Letters, 302, 274-278.
27. Török, K. & Trentham (1994). Mechanism of 2-Chloro-(∈-amino-Lys75-[6-4-(N,N-diethylamino)phenyl]-1,3,5-triazin-4-yl) calmodulin interactions with smooth muscle myosin light chain kinase and derived peptides. Biochemistry, 33, 12807-12830.
28. Wuthrich, K. (1986). NMR of Proteins and Nucleic Acids, Wiley, New York.
29. Yu, M. H, Weissman, J. S. & Kim, P. S. (1995). Contribution of individual side-chains to the stability of BPTI examined by alanine-scanning mutagenesis. J. Mol. Biol. 249, 388-397.