siRNAs targeting terminal sequences of the SARS-associated coronavirus membrane gene inhibit M protein expression through degradation of M mRNA

Journal of Virological Methods

Volumn 145, Issue 2, 2007, Pages 146-154

  Qin, Zhao ling ^a   Zhao, Ping ^a   Cao, Ming mei ^a   Qi, Zhong tian ^a  

^a SECOND MILITARY MEDICAL UNIVERSITY   (China)

Author keywords

Antiviral effect; M glycoprotein; RNA interference; SARS associated coronavirus; siRNA

Indexed keywords

EPIDERMAL GROWTH FACTOR RECEPTOR; GLYCOPROTEIN; GREEN FLUORESCENT PROTEIN; M PROTEIN; MESSENGER RNA; SMALL INTERFERING RNA;

ARTICLE; CELLULAR DISTRIBUTION; CONTROLLED STUDY; GENE SEQUENCE; GENE SILENCING; GENE TARGETING; GENETIC TRANSCRIPTION; GENETIC TRANSFECTION; GOLGI COMPLEX; HUMAN; HUMAN CELL; IN VITRO STUDY; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN LOCALIZATION; RNA TRANSCRIPTION; SARS CORONAVIRUS;

BASE SEQUENCE; CELL LINE; GENES, VIRAL; HUMANS; MOLECULAR SEQUENCE DATA; RNA INTERFERENCE; RNA STABILITY; RNA, MESSENGER; RNA, SMALL INTERFERING; RNA, VIRAL; SARS VIRUS; TRANSFECTION; VIRAL MATRIX PROTEINS;

SARS CORONAVIRUS;

EID: 34548272615     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2007.05.017     Document Type: Article

References (45)

1. Cao M.M., Ren H., Pan X., Pan W., and Qi Z.T. Inhibition of EGFP expression by siRNA in EGFP-stably expressing Huh-7 cells. J. Virol. Methods 119 (2004) 189-194
2. De Clercq E. Potential antivirals and antiviral strategies against SARS coronavirus infections. Expert Rev. Anti Infect. Ther. 4 (2006) 291-302
3. Donze O., and Picard D. RNA interference in mammalian cells using siRNAs synthesized with T7 RNA polymerase. Nucleic Acids Res. 30 (2002) e46
4. Dy kxhoorn D.M., Novina C.D., and Sharp P.A. Killing the message short RNAs that silence gene expression. Nat. Rev. Mol. Cell Biol. 4 (2003) 457-465
5. Elbashir S.M., Harborth J., Lendeckel W., Yalcin A., Weber K., and Tuschl T. Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature 411 (2001) 494-498
6. Fire A., Xu S., Montgomery M.K., Kostas S.A., Driver S.E., and Mello C.C. Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature 391 (1998) 806-811
7. Fouchier R.A., Kuiken T., Schutten M., van Amerongen G., van Doornum G.J., vanden Hoogen B.G., Peiris M., Lim W., Stohr K., and Osterhaus A.D. Aetiology: Koch's postulates fulfilled for SARS virus. Nature 423 (2003) 240
8. He M.L., Zheng B.J., Chen Y., Wong K.L., Huang J.D., Lin M.C., Peng Y., Yuen K.Y., Sung J.J., and Kung H.F. Kinetics and synergistic effects of siRNAs targeting structural and replicase genes of SARS-associated coronavirus. FEBS Lett. 580 (2006) 2414-2420
9. He R., Leeson A., Ballantine M., Andonov A., Baker L., Dobie F., Li Y., Bastien N., Feldmann H., Strocher U., Theriault S., Cutts T., Cao J., Booth T.F., Plummer F.A., Tyler S., and Li X. Characterization of protein-protein interactions between the nucleocapsid protein and membrane protein of the SARS coronavirus. Virus Res. 105 (2004) 121-125
10. Ketzinel-Gilad M., Shaul Y., and Galun E. RNA interference for antiviral therapy. J. Gene Med. 8 (2006) 933-950
11. Klumperman J., Locker J.K., Meijer A., Horzinek M.C., Geuze H.J., and Rottier P.J. Coronavirus M proteins accumulate in the Golgi complex beyond the site of virion budding. J. Virol. 68 (1994) 6523-6534
12. Kuiken T., Fouchier R.A., Schutten M., Rimmelzwaan G.F., van Amerongen G., van Riel D., Laman J.D., de Jong T., van Doornum G., Lim W., Ling A.E., Chan P.K., Tam J.S., Zambon M.C., Gopal R., Drosten C., van der Werf S., Escriou N., Manuquerra J.C., Stohr K., Peiris J.S., and Osterhaus A.D. Newly discovered coronavirus as the primary cause of severe acute respiratory syndrome. Lancet 362 (2003) 263-270
13. Kuo L., and Masters P.S. Genetic evidence for a structural interaction between the carboxy termini of the membrane and nucleocapsid proteins of mouse hepatitis virus. J. Virol. 76 (2002) 4987-4999
14. Li B.J., Tang Q., Cheng D., Qin C., Xie F.Y., Wei Q., Xu J., Liu Y., Zheng B.J., Woodle M.C., Zhong N., and Lu P.Y. Using siRNA in prophylactic and therapeutic regimens against SARS coronavirus in Rhesus macaque. Nat. Med. 11 (2005) 944-951
15. Li T., Zhang Y., Fu L., Yu C., Li X., Li Y., Zhang X., Rong Z., Wang Y., Ning H., Liang R., Chen W., Babiuk L.A., and Chang Z. siRNA targeting the leader sequence of SARS-CoV inhibits virus replication. Gene Ther. 12 (2005) 751-761
16. Livak K.J., and Schmittgen T.D. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods 25 (2001) 402-408
17. Lois C., Hong E.J., Pease S., Brown E.J., and Baltimore D. Germline transmission and tissue-specific expression of transgenes delivered by lentiviral vectors. Science 295 (2002) 868-872
18. Lu A., Zhang H., Zhang X., Wang H., Hu Q., Shen L., Schaffhausen B.S., Hou W., and Li L. Attenuation of SARS coronavirus by a short hairpin RNA expression plasmid targeting RNA-dependent RNA polymerase. Virology 324 (2004) 84-89
19. Meng B., Lui Y.W., Meng S., Cao C., and Hu Y. Identification of effective siRNA blocking the expression of SARS viral envelope E and RDRP genes. Mol. Biotechnol. 33 (2006) 141-148
20. Miyagishi M., and Taira K. Development and application of siRNA expression vector. Nucleic Acids Res. Suppl. (2002) 113-114
21. Murakami M., Ota T., Nukuzuma S., and Takegami T. Inhibitory effect of RNAi on Japanese encephalitis virus replication in vitro and in vivo. Microbiol. Immunol. 49 (2005) 1047-1056
22. Nal B., Chan C., Kien F., Siu L., Tse J., Chu K., Kam J., Staropoli I., Crescenzo-Chaigne B., Escriou N., van der Werf S., Yuen K.Y., and Altmeyer R. Differential maturation and subcellular localization of severe acute respiratory syndrome coronavirus surface proteins S, M and E. J. Gen. Virol. 86 (2005) 1423-1434
23. Ni B., Shi X., Li Y., Gao W., Wang X., and Wu Y. Inhibition of replication and infection of severe acute respiratory syndrome-associated coronavirus with plasmid-mediated interference RNA. Antivir. Ther. 10 (2005) 527-533
24. Nicholls J.M., Poon L.L., Lee K.C., Ng W.F., Lai S.T., Leung C.Y., Chu C.M., Hui P.K., Mak K.L., Lim W., Yan K.W., Chan K.H., Tsang N.C., Guan Y., Yuen K.Y., and Peiris J.S. Lung pathology of fatal severe acute respiratory syndrome. Lancet 361 (2003) 1773-1778
25. Oostra M., de Haan C.A., de Groot R.J., and Rottier P.J. Glycosylation of the severe acute respiratory syndrome coronavirus triple-spanning membrane proteins 3a and M. J. Virol. 80 (2006) 2326-2336
26. Peiris J.S., et al. Coronavirus as a possible cause of severe acute respiratory syndrome. Lancet 361 (2003) 1319-1325
27. Ping Y.H., Chu C.Y., Cao H., Jacque J.M., Stevenson M., and Rana T.M. Modulating HIV-1 replication by RNA interference directed against human transcription elongation factor SPT5. Retrovirology 1 (2004) 46
28. Qin Z.L., Zhao P., Zhang X.L., Yu J.G., Cao M.M., Zhao L.J., Luan J., and Qi Z.T. Silencing of SARS-CoV spike gene by small interfering RNA in HEK 293T cells. Biochem. Biophys. Res. Commun. 324 (2004) 1186-1193
29. Rajeevan M.S., Vernon S.D., Taysavang N., and Unger E.R. Validation of array-based gene expression profiles by real-time (kinetic) RT-PCR. J. Mol. Diagn. 3 (2001) 26-31
30. Rota P.A., Oberste M.S., Monroe S.S., Nix W.A., and Campagnoli R. Characterization of a novel coronavirus associated with severe acute respiratory syndrome. Science 300 (2003) 1394-1399
31. Rubinson D.A., Dillon C.P., Kwiatkowski A.V., Sievers C., Yang L., Kopinja J., Rooney D.L., Ihrig M.M., McManus M.T., Gertler F.B., Scott M.L., and Van Parijs L. A lentivirus-based system to functionally silence genes in primary mammalian cells, stem cells and transgenic mice by RNA interference. Nat. Genet. 33 (2003) 401-406
32. Shen S., Lin P.S., Chao Y.C., Zhang A., Yang X.M., Lim S.G., Hong W., and Tan Y.J. The severe acute respiratory syndrome coronavirus 3a is a novel structural protein. Biochem. Biophys. Res. Commun. 330 (2005) 286-292
33. Shi Y. Mammalian RNAi for the masses. Trends Genet. 19 (2003) 9-12
34. Shi Y., Yang D.H., Xiong J., Jia J., Huang B., and Jin Y.X. Inhibition of genes expression of SARS coronavirus by synthetic small interfering RNAs. Cell Res. Commun. 15 (2005) 193-200
35. Sui G., Soohoo C., Affar el B., Gay F., Shi Y., Forrester W.C., and Shi Y. A DNA vector-based RNAi technology to suppress gene expression in mammalian cells. Proc. Natl. Acad. Sci. U.S.A. 99 (2002) 5515-5520
36. Svoboda P., Stein P., and Schultz R.M. RNAi in mouse oocytes and preimplantation embryos: effectiveness of hairpin dsRNA. Biochem. Biophys. Res. Commun. 87 (2001) 1099-1104
37. Tao P., Zhang J., Tang N., Zhang B.Q., He T.C., and Huang A.L. Potent and specific inhibition of SARS-CoV antigen expression by RNA interference. Chin. Med. J. (Engl.) 118 (2005) 714-719
38. Voss D., Kern A., Traggiai E., Eickmann M., Stadler K., Lanzavecchia A., and Becker S. Characterization of severe acute respiratory syndrome coronavirus membrane protein. FEBS Lett. 580 (2006) 968-973
39. Wang Z., Ren L., Zhao X., Hung T., Meng A., Wang J., and Chen Y.G. Inhibition of severe acute respiratory syndrome virus replication by small interfering RNAs in mammalian cells. J. Virol. 78 (2004) 7523-7527
40. Wilson J.A., Jayasena S., Khvorova A., Sabatinos S., Rodrigue-Gervais I.G., Arya S., Sarangi F., Harris-Brandts M., Beaulieu S., and Richardson C.D. RNA interference blocks gene expression and RNA synthesis from hepatitis C replicons propagated in human liver cells. Proc. Natl. Acad. Sci. U.S.A. 100 (2003) 2783-2788
41. Wu C.J., Huang H.W., Liu C.Y., Hong C.F., and Chan Y.L. Inhibition of SRAS-CoV replication by siRNA. Antivir. Res. 65 (2005) 45-48
42. Xuan B., Qian Z., Hong J., and Huang W. EsiRNAs inhibit hepatitis B virus replication in mice model more efficiently than synthesized siRNAs. Virus Res. 118 (2006) 150-155
43. Yang D., Buchholz F., Huang Z., Goga A., Chen C.Y., Brodsky F.M., and Bishop J.M. Short RNA duplexes produced by hydrolysis with Escherichia coli RNase III mediate effective RNA interference in mammalian cells. Proc. Natl. Acad. Sci. U.S.A. 99 (2002) 9942-9947
44. Zhu X.D., Dang Y., Feng Y., Li T., and Huang P.T. RNase III-prepared short interfering RNAs induce degradation of SARS-coronavirus mRNAs in human cells. Sheng Wu Gong Cheng Xue Bao 20 (2004) 484-489
45. Zhang Y., Li T., Fu L., Yu C., Li Y., Xu X., Wang Y., Ning H., Zhang S., Chen W., Babiuk L.A., and Chang Z. Silencing SARS-CoV spike protein expression in cultured cells by RNA interference. FEBS Lett. 560 (2004) 141-146