Attenuation of SARS coronavirus by a short hairpin RNA expression plasmid targeting RNA-dependent RNA polymerase

Virology

Volumn 324, Issue 1, 2004, Pages 84-89

  Lu, Aili ^a   Zhang, Huanqing ^a   Zhang, Xiaoyan ^a   Wang, Hongxia ^b   Hu, Qikuan ^a   Shen, Li ^a   Schaffhausen, Brian S ^c   Hou, Weimin ^a,c   Li, Linsong ^a  

^a PEKING UNIVERSITY HEALTH SCIENCE CENTER   (China)

^b National Institute for Communicable Disease Control and Prevention   (China)

^c TUFTS UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Coronavirus; RNA interference (RNAI); RNA dependent RNA polymerase (RDRP); Severe acute respiratory syndrome (SARS); Short hairpin RNA (shRNA); Short interfering RNA (siRNA)

Indexed keywords

RNA; RNA DIRECTED RNA POLYMERASE;

ARTICLE; CONTROLLED STUDY; GENE EXPRESSION; GENE TARGETING; HELA CELL; HUMAN; HUMAN CELL; NUCLEOTIDE SEQUENCE; PLAQUE FORMING CELL; PLASMID; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FUNCTION; RNA INTERFERENCE; RNA STRUCTURE; SARS CORONAVIRUS; VERO CELL; VIRUS GENE;

ANIMALS; BASE SEQUENCE; CERCOPITHECUS AETHIOPS; HELA CELLS; HUMANS; MOLECULAR SEQUENCE DATA; PLASMIDS; RNA INTERFERENCE; RNA REPLICASE; SARS VIRUS; SEVERE ACUTE RESPIRATORY SYNDROME; VERO CELLS;

ANIMALIA; CORONAVIRUS; SARS CORONAVIRUS;

EID: 2942558679     PISSN: 00426822     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.virol.2004.03.031     Document Type: Article

References (25)

1. Baulcombe D. RNA silencing. Curr. Biol. 12:2002;R82-R84
2. Chiu Y.T. Taiwanese scientists find genetic link to SARS. Nat. Med. 9:2003;1335
3. Elbashir S.M., Harborth J., Lendeckel W., Yalcin A., Weber K., Tuschl T. Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature. 411:2001;494-498
4. Elbashir S.M., Martinez J., Patikaniowska A., Lendeckel W., Tuschl T. Functional anatomy of siRNAs for mediating efficient RNAi in Drosophila melanogaster embryo lysate. EMBO J. 20:2001;6877-6888
5. Enserink M., Normile D. Infectious diseases. Search for SARS origins stalls. Science. 302:2003;766-767
6. Fire A., Xu S., Montgomery M.K., Kostas S.A., Driver S.E., Melloc C. Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature. 391:1998;806-811
7. Ge Q., McManus M.T., Nguyen T., Shen C.H., Sharp P.A., Eisen H.N., Chen J. RNA interference of influenza virus production by directly targeting mRNA for degradation and indirectly inhibiting all viral RNA transcription. Proc. Natl. Acad. Sci. U.S.A. 100:2003;2718-2723
8. Gitlin L., Karelsky S., Andino R. Short interfering RNA confers intracellular antiviral immunity in human cells. Nature. 418:2002;430-434
9. Guan Y., Zheng B.J., He Y.Q., Liu X.L., Zhuang Z.X., Cheung C.L., Luo S.W., Li P.H., Zhang L.J., Guan Y.J., Butt K.M., Wong K.L., Chan K.W., Lim W., Shortridge K.F., Yuen K.Y., Peiris J.S., Poon L.L. Isolation and characterization of viruses related to the SARS coronavirus from animals in southern China. Science. 302:2003;276-278
10. He M.L., Zheng B., Peng Y., Peiris J.S., Poon L.L., Yuen K.Y., Lin M.C., Kung H.F., Guan Y. Inhibition of SARS-associated coronavirus infection and replication by RNA interference. JAMA. 290:2003;2665-2666
11. Jacque J.M., Triques K., Stevenson M. Modulation of HIV-1replication by RNA interference. Nature. 418:2002;435-438
12. Jia Q., Sun R. Inhibition of gamma herpesvirus replication by RNA interference. J. Virol. 77:2003;3301-3306
13. Kadotani N., Nakayashiki H., Tosa Y., Mayama S. RNA silencing in the phytopathogenic fungus Magnaporthe oryzae. Mol. Plant-Microbe Interact. 16:2003;769-776
14. Kapadia S.B., Brideau-Andersen A., Chisari F.V. Interference of hepatitis C virus RNA replication by short interfering RNAs. Proc. Natl. Acad. Sci. U.S.A. 100:2003;2014-2018
15. Knipe D.M., Howley P.M. Field Virology 2001. 2001;Lippincott Williams & Wilkins, Philadelphia, PA. Chap. 35, 36
16. Lai M.M. SARS virus: the beginning of the unraveling of a new coronavirus. J. Biomed. Sci. 10:2003;664-675
17. Martina B.E., Haagmans B.L., Kuiken T., Fouchier R.A., Rimmelzwaan G.F., Van Amerongen G., Peiris J.S., Lim W., Osterhaus A.D. Virology: SARS virus infection of cats and ferrets. Nature. 425:2003;915
18. McCaffrey A.P., Meuse L., Pham T.T., Conklin D.S., Hannon G.J., Kay M.A. RNA interference in adult mice. Nature. 418:2002;38-39
19. Novina C.D., Murray M.F., Dykxhoorn D.M., Beresford P.J., Riess J., Lee S.K., Collman R.G., Lieberman J., Shankar P., Sharp P.A. siRNA-directed inhibition of HIV-1 infection. Nat. Med. 8:2002;681-686
20. Qin X.F., An D.S., Chen I.S.Y., Baltimore D. Inhibiting HIV-1 infection in human T cells by lentiviral-mediated delivery of small interfering RNA against CCR5. Proc. Natl. Acad. Sci. U.S.A. 100:2003;183-188
21. Randall G., Grakoui A., Rice C.M. Clearance of replicating hepatitis C virus replicon RNAs in cell culture by small interfering RNAs. Proc. Natl. Acad. Sci. U.S.A. 100:2003;235-240
22. Song E., Lee S.K., Wang J., Ince N., Ouyang N., Min J., Chen J., Shankar P., Lieberman J. RNA interference targeting Fas protects mice from fulminant hepatitis. Nat. Med. 9:2003;347-351
23. WHO, Cumulative Number of Reported Cases of Severe Acute Respiratory syndrome (SARS) (http://www.who.int/csr/sars/country/table2003_09_23/en/).
24. Xia H., Mao Q., Paulson H.L., Davidson B.L. siRNA mediated gene silencing in vitro and in vivo. Nat. Biotechnol. 20:2002;1006-1010
25. Yan L., Velikanov M., Flook P., Zheng W., Szalma S., Kahn S. Assessment of putative protein targets derived from the SARS genome. FEBS Lett. 554:2003;257-263