Protease activity in the medium of larch (Larix spec.) embryogenic suspension cultures and medium-protein stabilization by compatible solutes

Journal of Applied Botany and Food Quality

Volumn 81, Issue 1, 2007, Pages 56-61

  Westhofen, Philipp ^a   Ismail, Ghada ^a   Zoglauer, Kurt ^a,b   Boehm, Robert ^a  

^a UNIVERSITY OF BONN   (Germany)

^b HUMBOLDT UNIVERSITY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CONIFEROUS TREE; EMBRYO; ENZYME ACTIVITY; PROTEIN; STABILIZATION;

BACTERIA (MICROORGANISMS); EQUUS ASINUS; LARIX; LARIX SP.; NICOTIANA TABACUM;

EID: 34548203073     PISSN: 16139216     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (39)

1. ARCHAMBAULT, J., WILLIAMS, R.D., LAVOIE, L., PÉPIN, M-F., CHAVARIE, C., 1994: Production of somatic embryos in a helical ribbon impeller bioreactor. Biotechnol. Bioeng. 44, 930-943.
2. BARTH, S., HUHN, M., MATTHEY, B., KLIMKA, A., GALINSKI, E.A., ENGERT, A., 2000: Compatible-solute-supported periplasmic expression of functional recombinant proteins under stress conditions. Appl. Environ. Microb. 66, 1572-1579.
3. BATEMAN, K.S., CONGIU, M., TREGEAR, G.W., CLARKE, A.E., ANDERSON, M.A., 1997: Bacitracin significantly reduces degradation of peptides in plant cell cultures. Biotechnol. Bioeng. 53, 226-231.
4. BAUR, A., RESKI, R., GORR, G., 2005: Enhanced recovery of a secreted recombinant human growth factor using stabilizing additives and by coexpression of human serum albumin in the moss Physcomitrella patens. Plant Biotech. J. 3, 331-340.
5. BECWAR, M.R., MNOLAND, T.L., WYCKOFF, J.L., 1989: Maturiation, germination and conversion of Norway spruce (Picea abies L.) somatic embryos to plants. In vitro Cell Dev. Biol. - Plant 25, 575-580.
6. BODEUTSCH, T., JAMES, E.A., LEE, J.M., 2001: The effect of immobilization on recombinant protein production in plant cell culture. Plant Cell Rep. 20, 562-566.
7. BOHNERT, H.J., SHEN, B., 1999: Transformation and compatible solutes. Sci. Hortic. 78, 237-260.
8. BRADFORD, M.M., 1976: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254.
9. DORAN, P.M., 2000: Foreign protein production in plant tissue culture. Curr. Opinion Biotech. 11, 199-204.
10. FISCHER, R., LIAO, Y-C., DROSSARD, J., 1999: Affinity-purification of a TMV-specific recombinant full-size antibody from a transgenic tobacco suspension culture. J. Immunol. Meth. 226, 1-10.
11. GALINSKI, E.A., 1993: Compatible solutes of halophilic eubacteria- molecular principles, water-solute interaction, stress protection. Experientia 49, 487-496.
12. GALINSKI, E.A., KAUFMANN, M., SCHWARZ, T., VANGALA, M-D., 2000: Use of compatible solutes as inhibitors of enzymatic degradation of macromolecular biopolymers. European patent EP 1125583 A1.
13. ISMAIL, G., SCHNABL, H., ZOGLAUER, K., BOEHM, R., 2004: Agrobacterium-mediated transformation of Larix decidua: An assessment of factors influencing the efficiency of gus gene transfer. J. Appl. Botany 78, 83-90.
14. JAIN, S.M., 1998: Somatic embryogenesis in forest trees. Proc. of the 7th Int. Workshop of Bio-Refor., Manila.
15. JAMES, E.A., WANG, C.L., WANG, Z.P., REEVES, R., SHIN, J.H., MAGNUSON, N.S., LEE, J.M., 2000: Production and characterization of biologically active human GM-CSF secreted by genetically modified plant cells. Prot. Express. Purific. 19, 131-138.
16. KEMPF, B., BREMER, E., 1998: Uptake and synthesis of compatible solutes as microbial stress responses to high-osmolality environments. Arch. Microbiol. 170, 319-330.
17. KLIMASZEWSKA, K., 1989: Plantlet development from immature zygotic embryos of hybrid larch through somatic embryogenesis. Plant Sci. (Shannon) 63, 95-103.
18. KWON, T.H., SEO, J.E., KIM, J., LEE, J.H., JANG, Y.S., YANG, M.S., 2003a: Expression and secretion of the heterodimeric protein Interleukin-12 in plant cell suspension culture. Biotechnol. Bioeng. 81, 870-875.
19. KWON, T.-H., KIM, Y.-S., LEE, J.-H., YANG, M.-S., 2003b: Production and secretion of biologically active human granulocyte-macrophage colony stimulation factor in transgenic tomato suspension cultures. Biotechnol. Letters 25, 1571-1574.
20. LACOUNT, W., AN, G., LEE, J.M., 1997: The effect of polyvinylpyrrolidone (PVP) on the heavy chain monoclonal antibody production from plant suspension cultures. Biotechnol. Letters 19, 93-96.
21. LEE, S.-Y., KIM, Y.-H., ROH, Y.-S., MYOUNG, H.-J., LEE, K.-Y., KIM, D.-I., 2004: Bioreactor operation for transgenic Nicotiana tabacum cell cultures and continuous production of recombinant human granulocyte-macrophage colony-stimulating factor by perfusion culture. Enzyme Microb. Tech. 35, 663-671.
22. LOOPSTRA, C.A., 2000: Proteins of the conifer extracellular matrix. In: Jain, S.M. (ed.), Molecular Biology of Woody Plants Vol. 1, 287-297. Kluwer Academic, Dordrecht.
23. MCDONALD, K.A., HONG, L.M., TROMBLY, D.M., XIE, Q., JACKMAN, A.P., 2005: Production of human alpha-1-antitrypsin from transgenic rice cell culture in a membrane bioreactor. Biotechnol. Prog. 21, 728-734.
24. MITA, G., NOCCO, G., LEUCI, C., GRECO, V., RAMPINO, P., PERROTTA, C., 1997: Secreted heat shock proteins in sunflower suspension cell cultures. Plant Cell Rep. 16, 792-796.
25. NAKAYAMA, H., YOSHIDA, K., ONO, H., MUROOKA, Y., SHINMYO, A., 2000: Ectoine, the compatible solute from Halomonas elongata, confers hyperosmotic tolerance in cultured tobacco cells. Plant Physiol. 122, 1239-1247.
26. SAUER, T., GALINSKI, E.A., 1998: Bacterial milking: A novel bioprocess for production of compatible solutes. Biotechnol. Bioeng. 57, 306-313.
27. SHARP, J.M., DORAN, P.M., 2001a: Characterization of monoclonal antibody fragments produced by plant cells. Biotechnol. Bioeng. 73, 338-346.
28. SHARP, J.M., DORAN, P.M., 2001b: Strategies for enhancing monoclonal antibody accumulation in plant cell and organ cultures. Biotechnol. Prog. 19, 979-992.
29. SCHIERMEYER, A., SCHINKEL, H., APEL, S., FISCHER, R., SCHILLBERG, S., 2005: Production of Desmodus rotundas salivary plasminogen activator α1 (DSPAα1) in tobacco is hampered by proteolysis. Biotechnol. Bioeng. 89, 848-858.
30. SCHNOOR, M., VOSS, P., CULLEN, P., BOKING, T., GALLA, H.J., GALINSKI, E.A., LORKOWSKI, S., 2004: Characterization of the synthetic compatible solute homoectoine as a potent PCR enhancer. Biochem. Biophys. Res. Com. 322, 867-872.
31. SILVEIRA, V., FLOH, E.I.S, HANDRO, W., GUERRA, M.P., 2004: Effect of plant growth regulators on the cellular growth and levels of intracellular protein, starch and polyamines in embryogenic suspension cultures of Pinus taeda. Plant Cell Tiss. Org. Cult. 76, 53-60.
32. TARYONO, 2004: Somatische Embryogenese and Transformation bei der Europäischen Lärche (Larix decidua Mill.). PhD thesis 2000, Humboldt University of Berlin, Germany.
33. TSOI, B.M.-Y., DORAN, P.M., 2002: Effect of medium properties and additives on antibody stability and accumulation in suspended plant cell cultures. Biotechnol. Appl. Biochem. 35, 171-180.
34. VAN HENGEL, A.J., TADESSE, Z., IMMERZEEL, P., SCHOLS, H., VAN KAMMEN, A., DE VRIES, S.C., 2001: N-Acetylglucosamine and glucosamine-containing arabinogalactan proteins control somatic embryogenesis. Plant Physiol. 125, 1880-1890.
35. WAHL, M.F., AN, G.H., LEE, J.M., 1995: Effects of dimethyl-sulfoxide on heavy-chain monoclonal-antibody production from plant-cell culture. Biotechnol. Let. 17, 463-468.
36. WINK, M., 1994: The cell culture medium - a functional extracellular compartment of suspension-cultured cells. Plant Cell Tiss. Org. Cult. 38, 307-319.
37. WOJTASZEK, P., PISLEWSKA, M., BOLWELL, G.P., STOBIECKI, M., 1998: Secretion of stress-related proteins by suspension-cultured Lupinus albus cells. Acta Biochim. Pol. 45, 281-285.
38. WONGSAMUTH, R., DORAN, P.M., 1997: Production of monoclonal antibodies by tobacco hairy roots. Biotechnol. Bioeng. 54, 401-415.
39. ZOGLAUER, K., BEHREND, U., RAHMAT, A., ROSS, H., TARYONO, 2003: Somatic embryogenesis - the gate to biotechnology in conifers. In: Laimer, M., Rücker, W. (eds.), Plant tissue culture 100 years since Gottlieb Haberlandt, 175-202. Springer Press, Vienna, New York, 2003.