Production of Desmodus rotundus salivary plasminogen activator α1 (DSPAα1) in tobacco is hampered by proteolysis

Biotechnology and Bioengineering

Volumn 89, Issue 7, 2005, Pages 848-858

  Schiermeyer, Andreas ^a   Schinkel, Helga ^a   Apel, Stefanie ^a   Fischer, Rainer ^a   Schillberg, Stefan ^a  

^a RWTH AACHEN UNIVERSITY   (Germany)

Author keywords

BY 2 cells; Molecular farming; Proteolysis; Recombinant DSPA; Recombinant protein production; Suspension culture; Transgenic tobacco

Indexed keywords

BODY FLUIDS; CELLS; ENZYME KINETICS; EXTRACTION; IMMUNIZATION; PLANTS (BOTANY); PROTEINS;

ISCHEMIC STROKE; RECOMBINANT PROTEINS; SALIVARY PLASMINOGEN; TOBACCO PLANTS;

BIOMEDICAL ENGINEERING;

DESMOTEPLASE; EDETIC ACID; HISTIDINE; PROTEINASE INHIBITOR; RECOMBINANT PROTEIN; SIGNAL PEPTIDE;

AMINO TERMINAL SEQUENCE; ARTICLE; BAT; CELL LINE; CELL SUSPENSION; CHO CELL; CULTURE MEDIUM; DESMODUS ROTUNDUS; ENZYME ACTIVITY; FARMING SYSTEM; IMMUNOBLOTTING; NONHUMAN; NUCLEOTIDE SEQUENCE; PLANT CELL; PLANT LEAF; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN SYNTHESIS; PROTEIN TRANSPORT; SUPERNATANT; TOBACCO; TRANSGENIC PLANT;

BLOTTING, WESTERN; FEASIBILITY STUDIES; HISTIDINE; HYDROLYSIS; PLANT LEAVES; PLANTS, GENETICALLY MODIFIED; PLANTS, TOXIC; PLASMINOGEN ACTIVATORS; PROTEIN PROCESSING, POST-TRANSLATIONAL; RECOMBINANT PROTEINS; TOBACCO;

EID: 16444369701     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.20410     Document Type: Article

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