Expression and secretion of the heterodimeric protein interleukin-12 in plant cell suspension culture

Biotechnology and Bioengineering

Volumn 81, Issue 7, 2003, Pages 870-875

  Kwon, T H ^a   Seo, J E ^a   Kim, J ^a   Lee, J H ^b   Jang, Y S ^a   Yang, M S ^a  

^a Chonbuk National University   (South Korea)

^b Silla University   (South Korea)

Author keywords

IL 12; Plant cell culture; Sexual crossing

Indexed keywords

BIOASSAY; PLANTS (BOTANY); POLYPEPTIDES; PROTEINS;

TRANSGENIC PLANTS;

CELL CULTURE;

INTERLEUKIN 12; PROTEIN; PROTEIN SUBUNIT; SIGNAL PEPTIDE;

ARTICLE; BIOASSAY; CELL CULTURE; CELL SUSPENSION; CELLULAR DISTRIBUTION; CONTROLLED STUDY; CULTURE MEDIUM; NONHUMAN; PLANT CELL; PROTEIN EXPRESSION; TRANSGENIC PLANT;

CULTURE TECHNIQUES; DOSE-RESPONSE RELATIONSHIP, DRUG; EXTRACELLULAR SPACE; GELATIN; GENE EXPRESSION REGULATION, PLANT; HUMANS; INTERLEUKIN-12; PLANT LEAVES; PLANTS, GENETICALLY MODIFIED; PROTEIN ENGINEERING; RECOMBINANT PROTEINS; TOBACCO;

EID: 0037473195     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.10528     Document Type: Article

References (31)

1. Abrams JS. 1995. Immunoenzymeric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. In: Abrams JS, editor. Current protocols in immunology. New York: Wiley.
2. Alwine JC, Kemp DJ, Stark GR. 1997. Method for- detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes. Proc Natl Acad Sci USA 74: 5350-5354.
3. Battaglino RA, Huergo M, Pilosof AMR, Bartholoma G. 1991. Culture requirement for the production of protease by Aspergillus oryzae in solid state fermentation. Appl Microb Biotech 35:292-292.
4. Chan SH, Perussia B, Gupta JW, Kobayash M, Pospisil M, Yong HA, Wolf SF, Yong D, Clark SC, Trinchieri G. 1991. Induction of interferon gamma production by natural killer cell stimulatory factor: Characterization of the responder cells and synergy with other inducers. J Exp Med 173:869-79.
5. Doran PM. 2000. Foreign protein production in plant tissue cultures. Curr Opinion Biotechnol 11:199-204.
6. Fischer R, Schumann D, Zimmermann S, Drossard J, Sack M, Schillberg S. 1999a. Expression and characterization of bispecific single-chain Fv fragments produced in transgenic plants. Eur J Biochem 262:810-816.
7. Fischer R, Emans N, Schuster F, Hellwig S, Drossard J. 1999b. Towards molecular farming in the future: Using plant-cell-suspension cultures as bioreactors. Biotechnol Appl Biochem 30:109-112.
8. Fischer R, Liao Y, Drossard J. 1999c. Affinity-purification of a TMV-specific recombinant full-size antibody from a transgenic tobacco suspension culture. J Immunolog Methods 226:1-10.
9. Francisco JA, Gawlak SL, Miller M, Bathe J, Russell D, Chace D, Mixan B, Zhao L, Fell HP, Siegall CB. 1997. Expression and characterization of bryodin 1 and bryodin 1-based single-chain immunotoxin from tobacco cell culture. Bioconjugate Chem 8:708-713.
10. Gately MK, Wilson DE, Wong HL. 1986. Synergy between recombinant interleukin 2 (rIL-2) and IL 2-depleted lymphokine-containing supernatants in facilitating allogenic human cytolytic T lymphocyte responses in vitro. J Immunol 136:1274-82.
11. James EA, Wang C, Wang Z, Reeves R, Shin JH, Magnuson NS, Lee JM. 2000. Production and characterization of biologically active human GM-CSF secreted by genetically modified plant cells. Protein Expr Purif 19:131-138.
12. Kobayashi M, Fitz L, Ryan M, Hewick RM, Clark SC, Chang S, London R, Sherman F, Perussia B, Trinchieri G. 1989. Identification and purification of natural killer cell stimulatory factor (NKSF), a cytokine with multiple biologic effects on human lymphocytes. J Exp Med 170:827-45.
13. Kukla BA, Guilley HA, Jonard GX, Richards KE, Mundry KW. 1979. Characterization of long guanosine-free RNA sequences from the dahlemense and U2 strains of tobacco mosaic virus. Eur J Biochem 98:61-66.
14. LaCount W, An G, Lee JM. 1997. The effect of polyvinylpyrrolidone (PVP) on the heavy chain monoclonal antibody production from plant suspension cultures. Biotechnol Lett 19:93-96.
15. Lee JS, Choi SJ, Kang HS, Oh WG, Cho KH, Kwon TH, Kim DH, Jang YS, Yang MS. 1997. Establishment of a transgenic tobacco cell suspension culture system for producing murine granulocyte-macrophage colony stimulating factor. Mol Cells 7:783-787.
16. Lee JH, Kim NS, Kwon TH, Jang YS, Yang MS. 2002. Increased production of human granulocyte-macrophage colony stimulating factor (hGM-CSF) by the addition of stabilizing polymer in plant suspension cultures. J Biotechnol 96:205-211.
17. Liu F, Lee JM. 1999. Effect of culture conditions on monoclonal antibody production from genetically modified tobacco suspension cultures. Biotechnol Bioprocess Eng 4:259-263.
18. Magnuson NS, Linzmaier PM, Reeves R, An G, HayGlass K, Lee JM. 1998. Secretion of biologically active human interleukin-2 and interleukin-4 from genetically modified tobacco cells in suspension culture. Protein Expr Purif 13:45-52.
19. Odell JT, Nagy F, Chua NH. 1985. Identification of DNA sequences required for activity of the cauliflower mosaic virus 35S promoter. Nature 313:810-812.
20. Shrap J, Doran PM. 2001a. Strategies for enhancing monoclonal antibody accumulation in plant cell and organ cultures. Biotechnol Prog 17: 979-992.
21. Sharp J, Doran PM. 2001b. Characterization of monoclonal antibody fragments produced by plant cells. Biotechnol Bioeng 73:338-347.
22. Southern EM. 1975. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503-17.
23. Stern AS, Podlaski FJ, Hulmes JD, Pan YC, Quinn PM, Wolitzky AG, Familletti PC, Stemlo DL, Truitt T, Chizzonite R. 1990. Purification to homogeneity and partial characterization of cytotoxic lymphocyte maturation factor from human B-lymphoblastoid cells. Proc Natl Acad Sci USA 87:6808-6812.
24. Terashima M, Murai Y, Kawamura M, Nakanishi S, Stoltz T, Chen L, Drohan W, Rodriquez RL, Katoh S. 1999a. Production of functional human α1-antitrypsin by plant cell culture. Appl Microb Biotech 52: 516-523.
25. Terashima M, Ejiri Y, Hashikawa N, Yoshida H. 1999b. Effect of osmotic pressure on human α1-antitrypsin production by plant cell culture. Biochem Eng J 4:31-36.
26. Towbin H, Staehelin T, Gordon J. 1979. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Biotechnology 24:145-149.
27. Van Engelenet FA, Schouten A, Molthoff JW, Roosien J, Salinas J, Dirkse WG, Schots A, Bakker J, Gommers FJ, Jongsma MA, Bosch D, Stiekema WJ. 1994. Coordinate expression of antibody subunit genes yields high levels of functional antibodies in roots of transgenic tobacco. Plant Mol Biol 26:1701-1710.
28. Verdelhan MD, Gomord V, Bastin M, Faye L, Courtois D. 1999. Expression of a carrot invertase gene in tobacco suspension cells cultivated in batch and continuous culture conditions. J Biosci Bioeng 87:302-306.
29. Wernsman EA, Matzinger DF. 1980. Artificial hybridization and self-pollination (pp. 661-664). In: Fehr WR and Hadley HH, editors. Hybridization of crop plants. Madison, WI: American Society of Agronomy and Crop Science of America Publishers.
30. Wolf SF, Temple RA, Kobayashi M, Young D, Dicig M, Lowe L, Dzialo R, Fitz L, Ferenz C, Hewick RM. 1991. Cloning of cDNA for natural killer cell stimulatory factor, a heterodimeric cytokine with multiple biologic effects on T and natural killer cells. J Immunol 146: 3074-3081.
31. Wongsamuth R, Doran PM. 1997. Production of monoclonal antibodies by tobacco hairy roots. Biotechnol Bioeng 54:401-415.