Propionyl-L-carnitine prevents age-related myocardial remodeling in the rabbit

Journal of Cardiovascular Pharmacology

Volumn 50, Issue 2, 2007, Pages 168-175

  Orlandi, Augusto ^a,c   Francesconi, Arianna ^a   Ferlosio, Amedeo ^a   Lascio, Antonio Di ^a   Marcellini, Marcella ^b   Pisano, Claudio ^b   Spagnoli, Luigi Giusto ^a  

^a UNIVERSITY OF ROME TOR VERGATA   (Italy)

^b Sigma Tau SpA   (Italy)

^c Anatomic Pathology Institute   (Italy)

Author keywords

Aging; Cardiac fibroblasts; Endothelium; Fibrosis; Vascular cell adhesion molecule 1

Indexed keywords

CELL ANTIBODY; COLLAGEN TYPE 1; PROPIONYLCARNITINE; UNCLASSIFIED DRUG; VASCULAR CELL ADHESION MOLECULE 1; VASCULAR CELL ADHESION MOLECULE 1 ANTIBODY;

AGING; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; APOPTOSIS; ARTICLE; CELL MATURATION; CELLULAR DISTRIBUTION; CONTROLLED STUDY; FIBROBLAST; HEART LEFT VENTRICLE; HEART MUSCLE FIBROSIS; HEART PROTECTION; HEART VENTRICLE REMODELING; HISTOLOGY; MALE; MICROVASCULATURE; MORPHOMETRICS; NONHUMAN; NUCLEOTIDE SEQUENCE; PATHOGENESIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

AGING; ANIMALS; CARDIOTONIC AGENTS; CARNITINE; COLLAGEN; COLLAGEN TYPE I; DISEASE MODELS, ANIMAL; ENDOMYOCARDIAL FIBROSIS; FIBROBLASTS; IMMUNOHISTOCHEMISTRY; IN SITU NICK-END LABELING; MALE; RABBITS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SIGNAL TRANSDUCTION; VASCULAR CELL ADHESION MOLECULE-1; VENTRICULAR REMODELING;

EID: 34547955729     PISSN: 01602446     EISSN: None     Source Type: Journal    
DOI: 10.1097/FJC.0b013e31805d8ee9     Document Type: Article

References (79)

1. Eghbali M, Robinson TF, Seifter S, et al. Collagen accumulation in heart ventricles as a function of growth and aging. Cardiovasc Res. 1989;23:723-729.
2. Varagic J, Susic D, Frohlich E. Heart, aging, and hypertension. Curr Opin Cardiol. 2001;16:336-341.
3. Anversa P, Hiler B, Ricci R, et al. Myocyte cell loss and myocyte hypertrophy in the aging rat heart. J Am Coll Cardiol. 1986;8:1441-1448.
4. Gazoti Debessa CR, Mesiano Maifrino LB, Rodrigues de Souza R. Age related changes of the collagen network of the human heart. Mech Ageing Dev. 2001;122:1049-1058.
5. Orlandi A, Francesconi A, Marcellini M, et al. Role of ageing and coronary atherosclerosis in the development of cardiac fibrosis in the rabbit. Cardiovasc Res. 2004;64:544-552.
6. de Souza RR. Aging of myocardial collagen. Biogerontology. 2002;3:325-335.
7. Weber KT, Brilla CG, Janicki JS. Myocardial fibrosis: functional significance and regulatory factors. Cardiovasc Res. 1993;27:341-348.
8. Anversa P, Palackal T, Sonnenblick EH, et al. Myocyte cell loss and myocyte cellular hyperplasia in the hypertrophied aging rat heart. Circ Res. 1990;67:871-885.
9. Cohn JN, Ferrari R, Sharpe N. Cardiac remodeling-concepts and clinical implications: a consensus paper from an international forum on cardiac remodeling. Behalf of an International Forum on Cardiac Remodeling. J Am Coll Cardiol. 2000;35:569-582.
10. Bishop JE, Lindahl G. Regulation of cardiovascular collagen synthesis by mechanical load. Cardiovasc Res. 1999;42:27-44.
11. Besse S, Robert V, Assayag P, et al. Nonsynchronous changes in myocardial collagen mRNA and protein during aging: effect of DOCAsalt hypertension. Am J Physiol. 1994;267:H2237-2244.
12. Mays PK, McAnulty RJ, Campa JS, et al. Age-related changes in collagen synthesis and degradation in rat tissues. Importance of degradation of newly synthesized collagen in regulating collagen production. Biochem J. 1991;276:307-313.
13. Tomanek RJ. Effects of age and exercise on the extent of the myocardial capillary bed. Anat Rec. 1970;167:55-62.
14. Lakatta EG. Arterial and cardiac aging: major shareholders in cardiovascular disease enterprises: Part III: cellular and molecular clues to heart and arterial aging. Circulation. 2003;107:490-497.
15. O'Brien KD, Allen MD, McDonald TO, et al. Vascular cell adhesion molecule-1 is expressed in human coronary atherosclerotic plaques. Implications for the mode of progression of advanced coronary atherosclerosis. J Clin Invest. 1993;92:945-951.
16. Merat S, Fruebis J, Sutphin M, et al. Effect of aging on aortic expression of the vascular cell adhesion molecule-1 and atherosclerosis in murine models of atherosclerosis. J Gerontol A Biol Sci Med Sci. 2000;55:B85-94.
17. Morisaki N, Saito I, Tamura K, et al. New indices of ischemic heart disease and aging: studies on the serum levels of soluble intercellular adhesion molecule-1 (ICAM-1) and soluble vascular cell adhesion molecule-1 (VCAM-1) in patients with hypercholesterolemia and ischemic heart disease. Atherosclerosis. 1997;131:43-48.
18. Kacimi R, Karliner JS, Koudssi F, et al. Expression and regulation of adhesion molecules in cardiac cells by cytokines: response to acute hypoxia. Circ Res. 1998;82:576-586.
19. Susic D, Frohlich ED. Hypertension and the heart. Curr Hypertens Rep. 2000;2:565-569.
20. Bremer J. Carnitine-metabolism and functions. Physiol Rev. 1983;63:1420-1480.
21. Tanaka Y, Sasaki R, Fukui F, et al. Acetyl-L-carnitine supplementation restores decreased tissue carnitine levels and impaired lipid metabolism in aged rats. J Lipid Res. 2004;45:729-735.
22. El-Aroussy W, Rizk A, Mayhoub G, et al. Plasma carnitine levels as a marker of impaired left ventricular functions. Mol Cell Biochem. 2000;213:37-41.
23. Lango R, Smolenski RT, Narkiewicz M, et al. Influence of L-carnitine and its derivatives on myocardial metabolism and function in ischemic heart disease and during cardiopulmonary bypass. Cardiovasc Res. 2001;51:21-29.
24. Schonekess BO, Allard MF, Lopaschuk GD. Propionyl L-carnitine improvement of hypertrophied heart function is accompanied by an increase in carbohydrate oxidation. Circ Res. 1995;77:726-734.
25. Corsico N, Nardone A, Lucreziotti MR, et al. Effect of propionyl-L-carnitine in a rat model of peripheral arteriopathy: a functional, histologic, and NMR spectroscopic study. Cardiovasc Drugs Ther. 1993;7:241-251.
26. Cipolla MJ, Nicoloff A, Rebello T, et al. Propionyl-L-carnitine dilates human subcutaneous arteries through an endothelium-dependent mechanism. J Vasc Surg. 1999;29:1097-1103.
27. Spagnoli LG, Orlandi A, Marino B, et al. Propionyl-L-carnitine prevents the progression of atherosclerotic lesions in aged hyperlipemic rabbits. Atherosclerosis. 1995;114:29-44.
28. Orlandi A, Marcellini M, Pesce D, et al. Propionyl-L-carnitine reduces intimal hyperplasia after injury in normocholesterolemic rabbit carotid artery by modulating proliferation and caspase 3-dependent apoptosis of vascular smooth muscle cells. Atherosclerosis. 2002;160:81-89.
29. Spagnoli LG, Orlandi A, Mauriello A, et al. Aging and atherosclerosis in the rabbit. 1. Distribution, prevalence and morphology of atherosclerotic lesions. Atherosclerosis. 1991;89:11-24.
30. Junqueira LC, Bignolas G, Brentani RR. Picrosirius staining plus polarization microscopy, a specific method for collagen detection in tissue sections. Histochem J. 1979;11:447-455.
31. Thiedemann KU, Holubarsch C, Medugorac I, et al. Connective tissue content and myocardial stiffness in pressure overload hypertrophy. A combined study of morphologic, morphometric, biochemical, and mechanical parameters. Basic Res Cardiol. 1983;78:140-155.
32. Weibel ER. Stereological methods. Practical Methods for Biological Morphometry. Academic Press London/San Francisco; 1979.
33. Terman A, Dalen H, Eaton JW, et al. Aging of cardiac myocytes in culture: oxidative stress, lipofuscin accumulation, and mitochondrial turnover. Ann N Y Acad Sci. 2004;1019:70-77.
34. Lee G Luna. Manual of histological staining medhods of Armed Forces Institute of Pathology. Armed Forces Institute of Pathology (U.S.) New York: Blakiston Division, McGraw-Hill; 1968.
35. Spagnoli LG, Orlandi A, Santeusanio G. Foam cells of the rabbit atherosclerotic plaque arrested in metaphase by colchicine show a macrophage phenotype. Atherosclerosis. 1991;88:87-92.
36. Orlandi A, Marcellini M, Spagnoli LG. Aging influences development and progression of early aortic atherosclerotic lesions in cholesterol-fed rabbits. Arterioscler Thromb Vasc Biol. 2000;20:1123-1136.
37. Iwata A, Sai S, Nitta Y, et al. Liposome-mediated gene transfection of endothelial nitric oxide synthase reduces endothelial activation and leukocyte infiltration in transplanted hearts. Circulation. 2001;103:2753-2759.
38. Fruebis J, Gonzalez V, Silvestre M, et al. Effect of probucol treatment on gene expression of VCAM-1, MCP-1, and M-CSF in the aortic wall of LDL receptor-deficient rabbits during early atherogenesis. Arterioscler Thromb Vasc Biol. 1997;17:1289-1302.
39. Kajstura J, Zhang X, Liu Y, et al. The cellular basis of pacing-induced dilated cardiomyopathy. Myocyte cell loss and myocyte cellular reactive hypertrophy. Circulation. 1995;92:2306-2317.
40. Lakatta EG, Levy D. Arterial and cardiac aging: major shareholders in cardiovascular disease enterprises: Part II: the aging heart in health: links to heart disease. Circulation. 2003;107:346-354.
41. Hinz B, Gabbiani G. Cell-matrix and cell-cell contacts of myofibroblasts: role in connective tissue remodeling. Thromb Haemost. 2003;90:993-1002.
42. Weisbroth SF, Flatt RE, Kraus, AL. The biology of laboratory rabbit. New York: Academic Press; 1974.
43. Carre F, Lessard Y, Coumel P, et al. Spontaneous arrhythmias in various models of cardiac hypertrophy and senescence of rats. A Holter monitoring study. Cardiovasc Res. 1992;26:698-705.
44. Weber KT. Cardiac interstitium in health and disease: the fibrillar collagen network. J Am Coll Cardiol. 1989;13:1637-1652.
45. Medugorac I. Collagen type distribution in the mammalian left ventricle during growth and aging. Res Exp Med. 1982;180:255-262.
46. Zhang X, Chen X, Hong Q, et al. TIMP-1 promotes age-related renal fibrosis through upregulating ICAM-1 in human TIMP-1 transgenic mice. J Gerontol A Biol Sci Med Sci. 2006;61:1130-1143.
47. Serste T, Bourgeois N. Ageing and the liver. Acta Gastroenterol Belg. 2006;69:296-298.
48. Samuel CS, Zhao C, Yang Q, et al. The relaxin gene knockout mouse: a model of progressive scleroderma. J Invest Dermatol. 2005;125:692-699.
49. Samuel CS, Zhao C, Bathgate RA, et al. The relaxin gene-knockout mouse: a model of progressive fibrosis. Ann N Y Acad Sci. 2005;1041:173-181.
50. Weber KT, Sun Y, Tyagi SC, et al. Collagen network of the myocardium: function, structural remodeling and regulatory mechanisms. J Mol Cell Cardiol. 1994;26:279-292.
51. Shivakumar K, Dostal DE, Boheler K, et al. Differential response of cardiac fibroblasts from young adult and senescent rats to ANG II. Am J Physiol Heart Circ Physiol. 2003;284:H1454-459.
52. Broderick TL, Haloftis G, Paulson DJ. L-propionylcarnitine enhancement of substrate oxidation and mitochondrial respiration in the diabetic rat heart. J Mol Cell Cardiol. 1996;28:331-340.
53. Pasini E, Comini L, Ferrari R, et al. Effect of propionyl-L-carnitine on experimental induced cardiomyopathy in rats. Am J Cardiovasc Pathol. 1992;4:216-222.
54. Broderick TL, Driedzic W, Paulson DJ. Propionyl-L-carnitine effects on postischemic recovery of heart function and substrate oxidation in the diabetic rat. Mol Cell Biochem. 2000;206:151-157.
55. Ferrari R, Pasini E, Condorelli E, et al. Effect of propionyl-L-carnitine on mechanical function of isolated rabbit heart. Cardiovasc Drugs Ther. 1991;5:17-23.
56. Tamamori M, Ito H, Hiroe M, et al. Stimulation of collagen synthesis in rat cardiac fibroblasts by exposure to hypoxic culture conditions and suppression of the effect by natriuretic peptides. Cell Biol Int. 1997;21:175-180.
57. Packer L, Valenza M, Serbinova E, et al. Free radical scavenging is involved in the protective effect of L-propionyl-carnitine against ischemia-reperfusion injury of the heart. Arch Biochem Biophys. 1991;288:533-537.
58. Chakravarti B, Chakravarti DN. Oxidative Modification of Proteins: Age-Related Changes. Gerontology. 2006;53:128-139.
59. Squier TC. Oxidative stress and protein aggregation during biological aging. Exp Gerontol. 2001;36:1539-1550.
60. Suurmeijer AJ, Clement S, Francesconi A, et al. Alpha-actin isoform distribution in normal and failing human heart: a morphological, morphometric, and biochemical study. J Pathol. 2003;199:387-397.
61. Yu CM, Tipoe GL, Wing-Hon Lai K, et al. Effects of combination of angiotensin-converting enzyme inhibitor and angiotensin receptor antagonist on inflammatory cellular infiltration and myocardial interstitial fibrosis after acute myocardial infarction. J Am Coll Cardiol. 2001;38:1207-1215.
62. Kirkland G, Paizis K, Wu LL, et al. Heparin-binding EGF-like growth factor mRNA is upregulated in the peri-infarct region of the remnant kidney model: In vitro evidence suggests a regulatory role in myofibroblast transformation. Journal of the American Society of Nephrology. 1998;9:1464-1473.
63. Silver MA, Pick R, Brilla CG, et al. Reactive and reparative fibrillar collagen remodelling in the hypertrophied rat left ventricle: two experimental models of myocardial fibrosis. Cardiovasc Res. 1990;24:741-747.
64. Cook-Mills JM. VCAM-1 signals during lymphocyte migration: role of reactive oxygen species. Mol Immunol. 2002;39:499-508.
65. Bevilacqua MP, Nelson RM. Endothelial-leukocyte adhesion molecules in inflammation and metastasis. Thromb Haemost. 1993;70:152-154.
66. Tong Q, Zheng L, Lin L, et al. Hypoxia-induced mitogenic factor promotes vascular adhesion molecule-1 expression via the PI-3K/Akt-NFkappaB signaling pathway. Am J Respir Cell Mol Biol. 2006;35:444-456.
67. Cybulsky MI, Iiyama K, Li H, et al. A major role for VCAM-1, but not ICAM-1, in early atherosclerosis. J Clin Invest. 2001;107:1255-1262.
68. von Andrian UH, Mackay CR. T-cell function and migration. Two sides of the same coin. N Engl J Med. 2000;343:1020-1034.
69. Reckelhoff JF, Kellum JA, Blanchard EJ, et al. Changes in nitric oxide precursor, L-arginine, and metabolites, nitrate and nitrite, with aging. Life Sci. 1994;55:1895-1902.
70. Vanella A, Russo A, Acquaviva R, et al. L-propionyl-carnitine as superoxide scavenger, antioxidant, and DNA cleavage protector. Cell Biol Toxicol. 2000;16:99-104.
71. Maccari F, Arseni A, Chiodi P, et al. L-carnitine effect on plasma lipoproteins of hyperlipidemic fat-loaded rats. Lipids. 1987;22:1005-1008.
72. Savitha S, Tamilselvan J, Anusuyadevi M, et al. Oxidative stress on mitochondrial antioxidant defense system in the aging process: role of DL-alpha-lipoic acid and L-carnitine. Clin Chim Acta. 2005;355:173-180.
73. Bertelli A, Conte A, Ronca G, et al. Protective effect of propionyl carnitine against peroxidative damage to arterial endothelium membranes. Int J Tissue React. 1991;13:41-43.
74. Sethi R, Dhalla KS, Ganguly PK, et al. Beneficial effects of propionyl L-carnitine on sarcolemmal changes in congestive heart failure due to myocardial infarction. Cardiovasc Res. 1999;42:607-615.
75. Alvarez M, Malecot CO, Gannier F, et al. Antimony-induced cardiomyopathy in guinea-pig and protection by L-carnitine. Br J Pharmacol. 2005;144:17-27.
76. Hiatt WR. Carnitine and peripheral arterial disease. Ann N Y Acad Sci. 2004;1033:92-98.
77. Silvestro A, Brevetti G, Schiano V, et al. Adhesion molecules and cardiovascular risk in peripheral arterial disease. Soluble vascular cell adhesion molecule-1 improves risk stratification. Thromb Haemost. 2005;93:559-563.
78. Li H, Cybulsky MI, Gimbrone MA Jr, et al. An atherogenic diet rapidly induces VCAM-1, a cytokine-regulatable mononuclear leukocyte adhesion molecule, in rabbit aortic endothelium. Arterioscler Thromb. 1993;13:197-204.
79. Dallabrida SM, Rupnick MA. Vascular endothelium in tissue remodeling: implications for heart failure. Cold Spring Harb Symp Quant Biol. 2002;67:417-427.