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Volumn 111, Issue 27, 2007, Pages 7914-7927

Electrostatics of DNA complexes with cationic lipid membranes

Author keywords

[No Author keywords available]

Indexed keywords

BIOLOGICAL MEMBRANES; DIELECTRIC MATERIALS; ELECTROSTATICS; LIPIDS; POISSON EQUATION; POSITIVE IONS;

EID: 34547496911     PISSN: 15206106     EISSN: None     Source Type: Journal    
DOI: 10.1021/jp0700175     Document Type: Article
Times cited : (40)

References (106)
  • 27
    • 0029781698 scopus 로고    scopus 로고
    • Dan, N. Biophys. J. 1996, 71, 1267.
    • (1996) Biophys. J , vol.71 , pp. 1267
    • Dan, N.1
  • 30
    • 84906392492 scopus 로고    scopus 로고
    • In ref 27, for instance, it was suggested that upon DNA adsorption on a lipid membrane the packaging of lipids is perturbed, giving rise to attractive undulation-induced interactions between DNA rods competing with direct electrostatic rod-rod repulsion. In ref 28, the effect of rod-rod and membrane-membrane repulsive and rod-membrane attractive electrostatic interactions in a DNA-CL lamellar stack on the membrane conformational properties has been considered. It was suggested that rodmembrane attraction favors a compression of the stack in a direction perpendicular to the membrane: The undulations of neighboring membranes are phased so that they produce a better embracing of intercalated oppositely charged DNA rods. It was shown that isoelectric complexes are favored in the weak screening regime, while DNA negatively overcharged complexes minimize the electrostatic energy in the strong screening limit, when the screening length is much smaller than the separation between the membranes
    • 29 The mobility of lipids on the membrane surface was shown to result in a reduction of rod-rod electrostatic coupling across the membrane.
  • 47
    • 84906392493 scopus 로고    scopus 로고
    • On the contrary, for the hexagonal assembly of charged rods in electrolyte solution the electrostatic potential Ψ(r, 2ξ/κa, l1(κRs)K0(κr, K 1(κRs)I0(κaa)K1(κ Rs, K1(κa)I1(κRs, becomes more negative than ψ0(r) with compression of assembly, which results in nearly exponential growth of the rod energy in assembly; see ref 37
    • 0(r) with compression of assembly, which results in nearly exponential growth of the rod energy in assembly; see ref 37.
  • 58
    • 84906407395 scopus 로고    scopus 로고
    • Note that the DNA persistence length is strongly dependent on the concentration and the valence of counterions in electrolyte solution. The dependence of lp on n053 appears to be consistent with the Odijk-Skolnick-Fixman theory of the polyelectrolyte electrostatic persistence55with the DNA linear charge density renormalized due to the counterion condensation52 down to one charge per k along the DNA axis (see however the discussion in ref 56 on this subject, The persistence length reaches the limiting value of ∼500 Å at high salt concentrations ≳0.01-0.1 M, and it increases to 1000 Å at about 0.001 M NaCl.53 At 5-25 μM concentrations of multivalent DNA-condensing cations, such as cohexammine3+ and spermidine 3, the lp of DNA descreses 2-3 times as compared to its value in monovalent salt solutions. In the main text, the canonical value of lP ≈ 50 n
    • 56 see also ref 58. This effect might also be relavant to DNA confinement between positively charged membranes in DNA-CL complexes. This is however a subject for a separate investigation.
  • 64
    • 84906407396 scopus 로고    scopus 로고
    • 3, 60
    • 3, 60
  • 66
    • 84906378451 scopus 로고    scopus 로고
    • The degree of counterion released form a DNA rod adsorbed in the vicinity of an oppositely charged planar surface has been studied in low-salt limit in ref 62. It has been shown in particular that if the surface Gouy-Champan length is much larger than the rod radius weakly charged surfaces, most of the condensed cations still stay near the rod. However, when the charges on the surface are allowed to move freely, almost complete release of condensed counterions from the rod has been predicted for situations when the Gouy-Champan length is of the order of the rod radius
    • The degree of counterion released form a DNA rod adsorbed in the vicinity of an oppositely charged planar surface has been studied in low-salt limit in ref 62. It has been shown in particular that if the surface Gouy-Champan length is much larger than the rod radius (weakly charged surfaces), most of the condensed cations still stay near the rod. However, when the charges on the surface are allowed to move freely, almost complete release of condensed counterions from the rod has been predicted for situations when the Gouy-Champan length is of the order of the rod radius.
  • 70
    • 84906364151 scopus 로고    scopus 로고
    • In ref 66 the interaction between a uniformly charged rod and a fluid membrane with a charge density that depends on the pH level has been considered. The PB boundary value problem has been solved with the charge-regulation boundary conditions modified due to the mobility of lipids on the membrane. It was obtained in particular that, due to redistribution of mobile charges of both signs on the membrane, at small rod-membrane separations their electrostatic interaction energy decreases considerably, The energy can become even attractive for a negatively charged rod in front of on average negatively charged membrane due to a positively charged patch on the membrane underneath the DNA rod
    • In ref 66 the interaction between a uniformly charged rod and a fluid membrane with a charge density that depends on the pH level has been considered. The PB boundary value problem has been solved with the charge-regulation boundary conditions modified due to the mobility of lipids on the membrane. It was obtained in particular that, due to redistribution of mobile charges of both signs on the membrane, at small rod-membrane separations their electrostatic interaction energy decreases considerably, The energy can become even attractive for a negatively charged rod in front of on average negatively charged membrane due to a positively charged patch on the membrane underneath the DNA rod.
  • 101


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.