Localization of hCAP-18 on the surface of chemoattractant-stimulated human granulocytes: Analysis using two novel hCAP-18-specific monoclonal antibodies

Journal of Leukocyte Biology

Volumn 82, Issue 1, 2007, Pages 161-172

  Stie, Jamal ^a   Jesaitis, Andrew V ^a   Lord, Connie I ^a   Gripentrog, Jeannie M ^a   Taylor, Ross M ^a   Burritt, James B ^a   Jesaitis, Algirdas J ^a  

^a MONTANA STATE UNIVERSITY   (United States)

Author keywords

Cathelicidin; Exocytosis; Inflammation; Leukocyte

Indexed keywords

ALKALINE PHOSPHATASE; CHEMOATTRACTANT; CYTOCHALASIN; EPITOPE; FORMYLMETHIONYLLEUCYLPHENYLALANINE; HUMAN CATHELICIDIN ANTIMICROBIAL PROTEIN 18; LACTOFERRIN; MONOCLONAL ANTIBODY; MONOCLONAL ANTIBODY H7; MONOCLONAL ANTIBODY N9; SODIUM CHLORIDE; SODIUM HYDROXIDE; TRITON X 114; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ANIMAL EXPERIMENT; ARTICLE; BINDING SITE; CELL FRACTIONATION; CELL MEMBRANE; CELL STIMULATION; CELL SURFACE; CONTROLLED STUDY; CULTURE MEDIUM; DENSITY GRADIENT; DRUG MECHANISM; DRUG SYNTHESIS; FLOW CYTOMETRY; GRANULOCYTE; HUMAN; HUMAN TISSUE; IMMUNOBLOTTING; IMMUNOFLUORESCENCE MICROSCOPY; LEUKOCYTE ACTIVATION; MASS SPECTROMETRY; MOUSE; NEUTROPHIL; NONHUMAN; PRIORITY JOURNAL; SEDIMENTATION; SOLUBILIZATION; UPREGULATION;

ALKALINE PHOSPHATASE; ANTIBODIES, MONOCLONAL; ANTIMICROBIAL CATIONIC PEPTIDES; CHEMOTACTIC FACTORS; EPITOPE MAPPING; EPITOPES; GRANULOCYTES; HUMANS; LACTOFERRIN; N-FORMYLMETHIONINE LEUCYL-PHENYLALANINE; NEUTROPHILS; PROTEIN BINDING; PROTEIN TRANSPORT;

EID: 34347369774     PISSN: 07415400     EISSN: None     Source Type: Journal    
DOI: 10.1189/jlb.0906586     Document Type: Article

References (59)

1. Zasloff, M. (2002) Antimicrobial peptides of multicellular organisms. Nature 415, 389-395.
2. Tossi, A., Sandri, L., Giangaspero, A. (2000) Amphipathic, α-helical antimicrobial peptides. Biopolymers 55, 4-30.
3. Shai, Y. (2002) Mode of action of membrane active antimicrobial peptides. Biopolymers 66, 236-248.
4. Zanetti, M., Gennaro, R., Romeo, D. (1995) Cathelicidins: a novel protein family with a common proregion and a variable C-terminal antimicrobial domain. FEBS Lett. 374, 1-5.
5. Lehrer, R. I., Ganz, T. (2002) Cathelicidins: a family of endogenous antimicrobial peptides. Curr. Opin. Hematol. 9, 18-22.
6. Sorensen, O. E., Follin, P., Johnsen, A. H., Calafat, J., Tjabringa, G. S., Hiemstra, P. S., Borregaard, N. (2001) Human cathelicidin, hCAP-18, is processed to the antimicrobial peptide LL-37 by extracellular cleavage with proteinase 3. Blood 97, 3951-3959.
7. Oren, Z., Lerman, J. C., Gudmundsson, G. H., Agerberth, B., Shai, Y. (1999) Structure and organization of the human antimicrobial peptide LL-37 in phospholipid membranes: relevance to the molecular basis for its non-cell-selective activity. Biochem. J. 341, 501-513.
8. Kirikae, T., Hirata, M., Yamasu, H., Kirikae, F., Tamura, H., Kayama, F., Nakatsuka, K., Yokochi, T., Nakano, M. (1998) Protective effects of a human 18-kilodalton cationic antimicrobial protein (CAP18)-derived peptide against murine endotoxemia. Infect. Immun. 66, 1861-1868.
9. Yang, D., Chen, Q., Schmidt, A. P., Anderson, G. M., Wang, J. M., Wooters, J., Oppenheim, J. J., Chertov, O. (2000) LL-37, the neutrophil granule- and epithelial cell-derived cathelicidin, utilizes formyl peptide receptor-like 1 (FPRL1) as a receptor to chemoattract human peripheral blood neutrophils, monocytes, and T cells. J. Exp. Med. 192, 1069-1074.
10. Niyonsaba, F., Iwabuchi, K., Someya, A., Hirata, M., Matsuda, H., Ogawa, H., Nagaoka, I. (2002) A cathelicidin family of human antibacterial peptide LL-37 induces mast cell chemotaxis. Immunology 106, 20-26.
11. Elssner, A., Duncan, M., Gavrilin, M., Wewers, M. D. (2004) A novel P2X7 receptor activator, the human cathelicidin-derived peptide LL37, induces IL-1 β processing and release. J. Immunol. 172, 4987-4994.
12. Bals, R. (2000) Epithelial antimicrobial peptides in host defense against infection. Respir. Res. 1, 141-150.
13. Niyonsaba, F., Someya, A., Hirata, M., Ogawa, H., Nagaoka, I. (2001) Evaluation of the effects of peptide antibiotics human β-defensins-1/-2 and LL-37 on histamine release and prostaglandin D(2) production from mast cells. Eur. J. Immunol. 31, 1066-1075.
14. Davidson, D. J., Currie, A. J., Reid, G. S., Bowdish, D. M., MacDonald, K. L., Ma, R. C., Hancock, R. E., Speert, D. P. (2004) The cationic antimicrobial peptide LL-37 modulates dendritic cell differentiation and dendritic cell-induced T cell polarization. J. Immunol. 172, 1146-1156.
15. Koczulla, R., von Degenfeld, G., Kupatt, C., Krotz, F., Zahler, S., Gloe, T., Issbrucker, K., Unterberger, P., Zaiou, M., Lebherz, C., Karl, A., Raake, P., Pfosser, A., Boekstegers, P., Welsch, U., Hiemstra, P. S., Vogelmeier, C., Gallo, R. L., Clauss, M., Bals, R. (2003) An angiogenic role for the human peptide antibiotic LL-37/hCAP-18. J. Clin. Invest. 111, 1665-1672.
16. Heilborn, J. D., Nilsson, M. F., Kratz, G., Weber, G., Sorensen, O., Borregaard, N., Stahle-Backdahl, M. (2003) The cathelicidin anti-microbial peptide LL-37 is involved in re-epithelialization of human skin wounds and is lacking in chronic ulcer epithelium. J. Invest. Dermatol. 120, 379-389.
17. Adamus, G., Zam, Z. S., Arendt, A., Palczewski, K., McDowell, J. H., Hargrave, P. A. (1991) Anti-rhodopsin monoclonal antibodies of defined specificity: characterization and application. Vision Res. 31, 17-31.
18. Stie, J., Jesaitis, A. J. (2004) Preparation of secretory vesicle-free plasma membranes by isopycnic sucrose gradient fractionation of neutrophils by the gelatin method. Cytotechnology 46, 109-122.
19. Burritt, J. B., Foubert, T. R., Baniulis, D., Lord, C. I., Taylor, R. M., Mills, J. S., Baughan, T. D., Roos, D., Parkos, C. A., Jesaitis, A. J. (2003) Functional epitope on human neutrophil flavocytochrome b558. J. Immunol. 170, 6082-6089.
20. Burritt, J. B., Quinn, M. T., Jutila, M. A., Bond, C. W., Jesaitis, A. J. (1995) Topological mapping of neutrophil cytochrome b epitopes with phage-display libraries. J. Biol. Chem. 270, 16974-16980.
21. Taylor, R. M., Baniulis, D., Burritt, J. B., Gripentrog, J. M., Lord, C. I., Riesselman, M. H., Maaty, W. S., Bothner, B. P., Angel, T. E., Dratz, E. A., Linton, G. F., Malech, H. L., Jesaitis, A. J. (2006) Analysis of human phagocyte flavocytochrome b558 by mass spectrometry. J. Biol. Chem. 281, 37045-37056.
22. Burritt, J. B., Bond, C. W., Doss, K. W., Jesaitis, A. J. (1996) Filamentous phage display of oligopeptide libraries. Anal. Biochem. 238, 1-13.
23. Smith, G. P., Scott, J. K. (1993) Libraries of peptides and proteins displayed on filamentous phage. Methods Enzymol. 217, 228-257.
24. Borregaard, N., Tauber, A. I. (1984) Subcellular localization of the human neutrophil NADPH oxidase. b-Cytochrome and associated flavoprotein. J. Biol. Chem. 259, 47-52.
25. Cowland, J. B., Johnsen, A. H., Borregaard, N. (1995) hCAP-18, a cathelin/pro-bactenecin-like protein of human neutrophil specific granules. FEBS Lett. 368, 173-176.
26. Sorensen, O., Bratt, T., Johnsen, A. H., Madsen, M. T., Borregaard, N. (1999) The human antibacterial cathelicidin, hCAP-18, is bound to lipoproteins in plasma. J. Biol. Chem. 274, 22445-22451.
27. Jesaitis, A. J., Gizachew, D., Dratz, E. A., Siemsen, D. W., Stone, K. C., Burritt, J. B. (1999) Actin surface structure revealed by antibody imprints: evaluation of phage-display analysis of anti-actin antibodies. Protein Sci. 8, 760-770.
28. Sorensen, O. E., Gram, L., Johnsen, A. H., Andersson, E., Bangsboll, S., Tjabringa, G. S., Hiemstra, P. S., Malm, J., Egesten, A., Borregaard, N. (2003) Processing of seminal plasma hCAP-18 to ALL-38 by gastricsin: a novel mechanism of generating antimicrobial peptides in vagina. J. Biol. Chem. 278, 28540-28546.
29. Oseas, R., Yang, H. H., Baehner, R. L., Boxer, L. A. (1981) Lactoferrin: a promoter of polymorphonuclear leukocyte adhesiveness. Blood 57, 939-945.
30. Boxer, L. A., Haak, R. A., Yang, H. H., Wolach, J. B., Whitcomb, J. A., Butterick, C. J., Baehner, R. L. (1982) Membrane-bound lactoferrin alters the surface properties of polymorphonuclear leukocytes. J. Clin. Invest. 70, 1049-1057.
31. Luna, E. J., Fowler, V. M., Swanson, J., Branton, D., Taylor, D. L. (1981) A membrane cytoskeleton from Dictyostelium discoideum. I. Identification and partial characterization of an actin-binding activity. J. Cell Biol. 88, 396-409.
32. Steck, T. L., Yu, J. (1973) Selective solubilization of proteins from red blood cell membranes by protein perturbants. J. Supramol. Struct. 1, 220-232.
33. Andersson, E., Sorensen, O. E., Frohm, B., Borregaard, N., Egesten, A., Malm, J. (2002) Isolation of human cationic antimicrobial protein-18 from seminal plasma and its association with prostasomes. Hum. Reprod. 17, 2529-2534.
34. Sorensen, O., Cowland, J. B., Askaa, J., Borregaard, N. (1997) An ELISA for hCAP-18, the cathelicidin present in human neutrophils and plasma. J. Immunol. Methods 206, 53-59.
35. Malm, J., Sorensen, O., Persson, T., Frohm-Nilsson, M., Johansson, B., Bjartell, A., Lilja, H., Stahle-Backdahl, M., Borregaard, N., Egesten, A. (2000) The human cationic antimicrobial protein (hCAP-18) is expressed in the epithelium of human epididymis, is present in seminal plasma at high concentrations, and is attached to spermatozoa. Infect. Immun. 68, 4297-4302.
36. Bals, R., Wang, X., Zasloff, M., Wilson, J. M. (1998) The peptide antibiotic LL-37/hCAP-18 is expressed in epithelia of the human lung where it has broad antimicrobial activity at the airway surface. Proc. Natl. Acad. Sci. USA 95, 9541-9546.
37. Bals, R., Weiner, D. J., Moscioni, A. D., Meegalla, R. L., Wilson, J. M. (1999) Augmentation of innate host defense by expression of a cathelicidin antimicrobial peptide. Infect. Immun. 67, 6084-6089.
38. Hjelmeland, L. M., Nebert, D. W., Chrambach, A. (1979) Electrofocusing of integral membrane proteins in mixtures of zwitterionic and nonionic detergents. Anal. Biochem. 95, 201-208.
39. Hjelmeland, L. M., Chrambach, A. (1981) Electrophoresis and electrofocusing in detergent containing media: a discussion of basic concepts. Electrophoresis 2, I-II.
40. Hjelmeland, L. M., Chrambach, A. (1984) Solubilization of functional membrane proteins. Methods Enzymol. 104, 305-318.
41. Sallantin, M., Huet, J. C., Demarteau, C., Pernollet, J. C. (1990) Reassessment of commercially available molecular weight standards for peptide sodium dodecyl sulfate-polyacrylamide gel electrophoresis using electroblotting and microsequencing. Electrophoresis 11, 34-36.
42. Bals, R., Wilson, J. M. (2003) Cathelicidins - a family of multifunctional antimicrobial peptides. Cell. Mol. Life Sci. 60, 711-720.
43. Rose, K., Kocher, H. P., Blumberg, B. M., Kolakofsky, D. (1984) An improved procedure, involving mass spectrometry, for N-terminal amino acid sequence determination of proteins which are N α-blocked. Biochem. J. 217, 253-257.
44. Storici, P., Tossi, A., Lenarcic, B., Romeo, D. (1996) Purification and structural characterization of bovine cathelicidins, precursors of antimicrobial peptides. Eur. J. Biochem. 238, 769-776.
45. Lou, Y. C., Huang, Y. C., Pan, Y. R., Chen, C., Liao, Y. D. (2006) Roles of N-terminal pyroglutamate in maintaining structural integrity and pKa values of catalytic histidine residues in bullfrog ribonuclease 3. J. Mol. Biol. 355, 409-421.
46. Busby Jr., W. H., Quackenbush, G. E., Humm, J., Youngblood, W. W., Kizer, J. S. (1987) An enzyme(s) that converts glutaminyl-peptides into pyroglutamyl-peptides. Presence in pituitary, brain, adrenal medulla, and lymphocytes. J. Biol. Chem. 262, 8532-8536.
47. Kageyama, S., Tsomides, T. J., Fukusen, N., Papayannopoulos, I. A., Eisen, H. N., Sykulev, Y. (2001) Potent cytolytic response by a CD8+ CTL clone to multiple peptides from the same protein in association with an allogeneic class I MHC molecule. J. Immunol. 166, 3028-3034.
48. Sorensen, O. E. (2005) The human cathelicidin hCAP-18. Dan. Med. Bull. 52, 1-10.
49. Lara, L. L., Rivera, H., Perez, P., Blanca, I., Bianco, N. E., De Sanctis, J. B. (1997) Low density lipoprotein receptor expression and function in human polymorphonuclear leucocytes. Clin. Exp. Immunol. 107, 205-212.
50. Sengelov, H., Follin, P., Kjeldsen, L., Lollike, K., Dahlgren, C., Borregaard, N. (1995) Mobilization of granules and secretory vesicles during in vivo exudation of human neutrophils. J. Immunol. 154, 4157-4165.
51. Lollike, K., Lindau, M., Calafat, J., Borregaard, N. (2002) Compound exocytosis of granules in human neutrophils. J. Leukoc. Biol. 71, 973-980.
52. Sengelov, H., Kjeldsen, L., Borregaard, N. (1993) Control of exocytosis in early neutrophil activation. J. Immunol. 150, 1535-1543.
53. Borregaard, N., Kjeldsen, L., Sengelov, H., Diamond, M. S., Springer, T. A., Anderson, H. C., Kishimoto, T. K., Bainton, D. F. (1994) Changes in subcellular localization and surface expression of L-selectin, alkaline phosphatase, and Mac-1 in human neutrophils during stimulation with inflammatory mediators. J. Leukoc. Biol. 56, 80-87.
54. Bylund, J., Karlsson, A., Boulay, F., Dahlgren, C. (2002) Lipopolysaccharide-induced granule mobilization and priming of the neutrophil response to Helicobacter pylori peptide Hp(2-20), which activates formyl peptide receptor-like 1. Infect. Immun. 70, 2908-2914.
55. Manev, V., Maneva, A., Sirakov, L. (1998) Effect of lactoferrin on the phagocytic activity of polymorphonuclear leucocytes isolated from blood of patients with autoimmune diseases and Staphylococcus aureus allergy. Adv. Exp. Med. Biol. 443, 321-330.
56. Kai, K., Komine, K., Komine, Y., Kuroishi, T., Kozutsumi, T., Kobayashi, J., Ohta, M., Kitamura, H., Kumagai, K. (2002) Lactoferrin stimulates a Staphylococcus aureus killing activity of bovine phagocytes in the mammary gland. Microbiol. Immunol. 46, 187-194.
57. Bulow, E., Bengtsson, N., Calafat, J., Gullberg, U., Olsson, I. (2002) Sorting of neutrophil-specific granule protein human cathelicidin, hCAP-18, when constitutively expressed in myeloid cells. J. Leukoc. Biol. 72, 147-153.
58. Sorensen, O., Arnljots, K., Cowland, J. B., Bainton, D. F., Borregaard, N. (1997) The human antibacterial cathelicidin, hCAP-18, is synthesized in myelocytes and metamyelocytes and localized to specific granules in neutrophils. Blood 90, 2796-2803.
59. Elsbach, P., Weiss, J., Levy, O. (1999) Oxygen-independent antimicrobial systems of phagocytes. In Inflammation: Basic Principles and Clinical Correlates (J. I. Gallin, R. Snyderman, eds.), 3rd ed., Philadelphia, PA, USA, Lippincott, Williams, and Wilkins, 801-817.