Purification and structural characterization of bovine cathelicidins, precursors of antimicrobial peptides

European Journal of Biochemistry

Volumn 238, Issue 3, 1996, Pages 769-776

  Storici, Paola ^a   Tossi, Alessandro ^a   Lenarčič, Brigita ^b   Romeo, Domenico ^a  

^a UNIVERSITY OF TRIESTE   (Italy)

^b JO EF STEFAN INSTITUTE   (Slovenia)

Author keywords

antimicrobial peptide; cathelicidin; cystatin superfamily; kyninogen; mass spectrometry

Indexed keywords

CATHELICIDIN; CYSTATIN; PROTEIN PRECURSOR; PYROGLUTAMIC ACID; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ANIMAL CELL; ARTICLE; BONE MARROW CELL; CATTLE; DIMERIZATION; DISULFIDE BOND; DNA SEQUENCE; MASS SPECTROMETRY; NONHUMAN; PEPTIDE SYNTHESIS; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN PURIFICATION;

ANIMALIA; BOS TAURUS; BOVINAE; MAMMALIA;

EID: 0030057777     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1432-1033.1996.0769w.x     Document Type: Article

References (55)

1. Agerberth, B., Gunne, H., Odeberg, J., Kogner, P., Boman, H. G. & Gudmundsson, G. H. (1995) FALL-39, a putative human peptide antibiotic, is cysteine-free and expressed in bone marrow and testis, Proc. Natl Acad. Sci. USA 92, 195-199.
2. Bagella, L., Scocchi, M. & Zanetti, M (1995) cDNA sequences of three sheep myeloid cathelicidins, FEBS Lett. 376, 225-228.
3. Barra, D. & Simmaco, M. (1995) Amphibian skin: a promising resource for antimicrobial peptides, Tibtech 13, 205-209.
4. Barrett, A. J. (1972) A new assay for cathepsin B1 and other thiol proteinases, Anal. Biochem. 47, 280-289.
5. Bieth, J. G. (1984) In vivo significance of kinetic constants of protein proteinase inhibitors, Biochem. Med. 32, 387-397.
6. Bode, W., Engh, R., Musil, D., Thiele, U., Huber, R., Karshikov, A., Brzin, J., Kos, J. & Turk, V. (1988) The 2.0 angstroms X-ray crystal structure of chicken egg white cystatin and its possible mode of interaction with cysteine proteinases, EMBO J. 7, 2593-2599.
7. Boman, H. G. (1995) Peptide antibiotics and their role in innate immunity, Annu. Rev. Immunol. 13, 61-92.
8. Borregaard, N., Hieple, J. M., Simons, E. R. & Clark, R. A., (1983) Subcellular localization of the b-Cytochrome component of the human neutrophil microbicidal oxidase: translocation during activation, J. Cell. Biol. 97, 56-61.
9. Chen, Y.-H., Yang, J. T. & Chau, K. H. (1974) Determination of the helix and β form of proteins in aqueous solution by circular dichroism, Biochemistry 13, 3350-3359.
10. Cowland, J. B., Johnsen, A. H. & Borregaard, N. (1995) hCAP-18, a cathelin/pro-bactenecin-like protein of human neutrophil specific granules, FEBS Lett. 368, 173-176.
11. Del Sal, G., Storici, P., Schneider, C., Romeo, D. & Zanetti, M. (1992) cDNA cloning of the neutrophil bactericidal peptide indolicidin, Biochem. Biophys. Res. Commun. 187, 467-472.
12. Frank, R. W., Gennaro, R., Schneider, K., Przybylski, M. & Romeo, D. (1990) Amino acid sequences of two proline-rich bactenecins, antimicrobial peptides of bovine neutrophils, J. Biol. Chem. 265, 18871-18874.
13. Gallis, B., Mehl, J., Prickett, K. S., Martin, J. A., Merriam, J., March, C. J. & Cerretti, D. P. (1989) Antimicrobial activity of synthetic bactenecin, Biotech. Therap. 1, 335-346.
14. Ganz, T. (1994) Biosynthesis of defensins and other antimicrobial peptides, Ciba Found. Symp. 186, 62-76.
15. Gennaro, R., Zanetti, M. & Romeo, D. (1996) Gene encoded antibacterial peptides from animals, in Biotechnological aspects of industrial antibiotics (Lal, R. and Lal, S., eds) Springer-Verlag, Berlin, in press.
16. Gudmundsson, H. G., Magnusson, K. P., Chowdhary, B. P., Johansson, M., Andersson, L. & Boman, H. (1995) Structure of the gene for porcine peptide antibiotic PR-39, a cathelin gene family member: Comparative mapping of the locus for the human peptide antibiotic FALL-39, Proc. Natl Acad. Sci. USA 92, 7085-7089.
17. Hu, B., Coulson, L., Moyer, B. & Price, P. (1995) Isolation and molecular cloning of a novel bone phosphoprotein related in sequence to the cystatin family of thiol protease inhibitors, J. Biol. Chem. 270, 431-436.
18. Jones, D. T., Taylor, W. R. & Thornton, J. N. (1992) A new approach to protein fold recognition, Nature 358, 86-89.
19. Kopitar, M., Ritonja, A., Popovic, T., Gabrijeleic, D., Krizaj, I. & Turk, V. (1989) A new type of low-molecular mass cysteine proteinase inhibitor from pig leukocytes, Biol. Chem. Hoppe-Seyler 370, 1145-1151.
20. Landon, M. (1977) Cleavage at aspartyl-prolyl bonds, Methods Enzymol. 47, 145-149.
21. Larrick, J. W., Morgan, J. G., Palings, I., Hirata, M. & Yen, M. H. (1991) Complementary DNA sequence of rabbit CAP18, a unique lipopolysaccharide binding protein, Biochem. Biophys. Res. Commun. 179, 170-175.
22. Larrick, J. W., Hirata, M., Balint, R. F., Lee, J., Zhong, J. & Wright, S. C. (1995) Human CAP18: a novel antimicrobial lipopolysaccharidebinding protein, Infect. Immun. 63, 1291-1297.
23. Lenarčič, B., Ritonja, A., Dolenc, I., Stoka, V., Berbic, S., Pungercar, J., Strukely, B. & Turk, V. (1993) Pig leukocyte cysteine proteinase inhibitor (PLCPI), a new member of the stefin family, FEBS Lett. 336, 289-292.
24. Levy, O., Weiss, J., Zarember, K., Ooi, C. E. & Elsbach, P. (1993) Antibacterial 15-kDa protein isoforms (p15) are members of a novel family of leukocyte proteins, J. Biol. Chem. 268, 6058-6063.
25. Mottola, C., Gennaro, R., Marzullo, A. & Romeo, D. (1980) Isolation and partial characterization of the plasma membrane of purified bovine neutrophils, Eur. J. Biochem. 111, 341-346.
26. Provencher, S. W. & Glöckner, J. (1981) Estimation of globular protein secondary structure from circular dichroism, Biochemistry 20, 33-37.
27. Pungercar, J., Strukelj, B., Kopitar, G., Renko, M. Lenarčič, B., Gubensek, F. & Turk, V. (1993) Molecular cloning of a putative homolog of proline/arginine-rich antibacterial peptides from porcine bone marrow, FEBS Lett. 336, 1284-288.
28. Rawlings, N. D. & Barrett, A. J. (1990) Evolution of proteins of the cystatin superfamily, J. Mol. Evol. 30, 60-71.
29. Ritonja, A., Kopitar, M., Jerala, R. & Turk, V. (1989) Primary structure of a new cysteine proteinase inhibitor from pig leukocytes, FEBS Lett. 255, 211-214.
30. Romeo, D., Skerlavaj, B., Bolognesi, M. & Gennaro, R. (1988) Structure and bactercidal activity of an antibiotic dodecapeptide purified from bovine neutrophils, J. Biol. Chem. 263, 9573-9575.
31. Rost, B. & Sanders, C. (1994) Combining evolutionary information and neural networks to predict protein secondary structure, Proteins 19, 55-72.
32. Rost, B. (1995) Fitting ID predictions into 3D structures, in Protein folds. A distance-based approach (Bohr, H. & Brunak, S., eds) pp. 132-151, CRC Press, Boca Raton, FL.
33. Scocchi, M., Skerlavaj, B., Romeo, D. & Gennaro, D. (1992) Proteolytic cleavage by neutrophil elastase converts inactive storage proforms to antibacterial bactenecins, Eur. J. Biochem. 209, 589-595.
34. Scocchi, M., Romeo, D. & Zanetti, M. (1994) Molecular cloning of Bac7. a proline- and arginine-rich antimicrobial peptide from bovine neutrophils, FEBS Lett. 352, 197-200.
35. Selsted, M. E. & Ouellette, A. J. (1995) Defensins in granules of phagocytic and non-phagocytic cells, Trends Cell Biol. 5, 114-119.
36. Smith, P. K., Krohn, R. I., Hermanson, G. T., Mallia, A. K., Gartner, F. H., Provenzano, M. D., Fujimoto, E. K., Goeke, N. M., Olson, B. J. & Klenk, D. C. (1985) Measurement of protein using bicinchoninic acid, Anal. Biochem. 150, 76-85.
37. Storici, P., Del Sal, G., Schneider, C. & Zanetti, M. (1992) cDNA sequence analysis of an antibiotic dodecapeptide from neutrophils, FEBS Lett. 314, 187-190.
38. Storici, P. & Zanetti, M. (1993a) A cDNA derived from pig bone marrow cells contains a sequence identical to the intestinal antibacterial peptide PR-39, Biochem. Biophys. Res. Commun. 196, 1058-1065.
39. Storici, P. & Zanetti, M. (1993b) A novel cDNA sequence encoding a pig antimicrobial peptide with a cathelin-like pro-scquenco, Biochem. Biophys. Res. Commun. 196, 1363-1368.
40. Storici, P., Scocchi, M., Tossi, A., Gennaro, R. & Zanetti, M. (1994) Chemical synthesis and biological activity of a novel antibacterial peptide deduced from a pig myeloid cDNA, FEBS Lett. 337, 303-307.
41. Sturrock, S. S. & Collins, J. E. (1993) MPsrch V1.3, Biocomputing Research Unit, University of Edinburgh.
42. Tam, P. J. (1988) Synthetic peptide vaccine design: synthesis and properties of a high-density multiple antigenic peptide system, Proc. Natl Acad. Sci. USA 85, 5409-5413.
43. Tossi, A., Scocchi, M. Zanetti, M., Storici, P. & Gennaro, R. (1995) Porcine myeloid antibacterial peptide, PMAP-37, a novel antibacterial peptide from pig myeloid cells: cDNA cloning, chemical synthesis and activity, Eur. J. Biochem. 228, 941-946.
44. Turk, B., Dolenc, I., Turk, V. & Bieth, J. G. (1993) Kinetics of the pH-induced inactivation of human cathepsin L, Biochemistry 32, 375-380.
45. Turk, V. & Bode, W. (1991) The cystatins: protein inhibitors of cysteine proteinases, FEBS Lett. 285, 213-219.
46. Verbanac, D., Zanetti, M. & Romeo, D. (1993) Chemotactic and protease-inhibiting activities of antibiotic peptide precursors, FEBS Lett. 317, 255-258.
47. Waddell, W. J. (1956) A simple ultraviolet spectrophotometric method for the determination of protein, J. Lab. Clin. Med. 48, 311-314.
48. Zanetti, M., Litteri, L., Gennaro, R., Horstmann, H. & Romeo, D. (1990) Bactenecins, defense polypeptides of bovine neutrophils, are generated from precursor molecules stored in the large granules, J. Cell Biol. 111, 1363-1371.
49. Zanetti, M., Litteri, L., Griffiths, G., Gennaro, R. & Romeo, D. (1991) Stimulus induced maturation of probactenecins, precursors of neutrophil antimicrobial polypeptides, J. Immunol. 146, 4295-4300.
50. Zanetti, M., Del Sal, G., Storici, P., Schneider, C. & Romeo, D. (1993) The cDNA of the neutrophil antibiotic Bac5 predicts a pro-sequence homologous to a cysteine proteinase inhibitor, that is common to other neutrophil antibiotics, J. Biol. Chem. 268, 522-526.
51. Zanetti, M., Storici, P., Tossi, A., Scocchi, M. & Gennaro, R. (1994) Molecular cloning and chemical synthesis of a novel antibacterial peptide derived from pig myeloid cells, J. Biol. Chem. 269, 7855-7858.
52. Zanetti, M., Gennaro, R. & Romeo, D. (1995) Cathelicidins: a novel family of antimicrobial peptide precursors, FEBS Lett. 374, 1-5.
53. Zhao, C., Liu, L. & Lehrer, R. I. (1994) Identification of a new member of the protegrin family by cDNA cloning, FEBS Lett. 346, 285-288.
54. Zhao, C., Ganz, T. & Lehrer, R. I. (1995a) The structure of porcine protegrin genes, FEBS Lett. 368, 197-202.
55. Zhao, C., Ganz, T. & Lehrer, R. I. (1995b) Structures of genes for two cathelin assosciated antimicrobial peptides; prophenin-2 and PR-39, FEBS Lett. 376, 130-134.