An ELISA for hCAP-18, the cathelicidin present in human neutrophils and plasma

Journal of Immunological Methods

Volumn 206, Issue 1-2, 1997, Pages 53-59

  Sørensen, Ole ^a   Cowland, Jack B ^a   Askaa, Jon ^b   Borregaard, Niels ^a  

^a University of Copenhagen   (Denmark)

^b AGILENT TECHNOLOGIES   (United States)

Author keywords

Antibacterial peptide; Cathelicidin; ELISA; FALL39; hCAP 18; Neutrophil

Indexed keywords

ANTIMICROBIAL ACTIVITY; ARTICLE; ENZYME LINKED IMMUNOSORBENT ASSAY; HOST RESISTANCE; HUMAN; HUMAN CELL; NEUTROPHIL; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN BLOOD LEVEL;

ANIMALS; ANTI-INFECTIVE AGENTS; ANTIBODIES; ANTIMICROBIAL CATIONIC PEPTIDES; CARRIER PROTEINS; ENZYME-LINKED IMMUNOSORBENT ASSAY; HUMANS; NEUTROPHILS; PLASMA; RABBITS; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY;

EID: 0030805339     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(97)00084-7     Document Type: Article

References (20)

1. Agerberth, B., Gunne, H., Odeberg, J., Kogner, P., Boman, H.G., Gudmundsson, G.H., 1995. FALL-39, a putative human peptide antibiotic, is cysteine-free and expressed in bone marrow and testis. Proc. Natl. Acad. Sci. USA 92, 195.
2. Bayer, E.A., Wilchek, M., 1990. Protein biotinylation. Methods Enzymol. 184, 146-148.
3. Borregaard, N., Heiple, J.M., Simons, E.R., Clark, R.A., 1983. Subcellular localizations of the b-cytochrome component of the human neutrophil microbial oxidase: Translocation during activation. J. Cell Biol. 97, 52.
4. Borregaard, N., Kjeldsen, L., Rygaard, K., Bastholm, L., Nielsen, M.H., Sengeløv, H., Bjerrum, O.W., Johnson, A.M., 1992. Stimulus-dependent secretion of plasma proteins from human neutrophils. J. Clin. Invest. 90, 86.
5. Boyum, A., 1968. Isolation of mononuclear cells and granulocytes from human blood. Scand. J. Clin. Lab. Invest. 21, 77.
6. Cowland, J.B., Johnsen, A.H., Borregaard, N., 1995. hCAP-18, a cathelin/pro-bactenecin-like protein of human neutrophil specific granules. FEBS Lett. 368, 173.
7. Gudmundsson, H., Agerberth, B., Odeberg, J., Bergman, T., Olsson, B., Salcedo, R., 1996. The human gene FALL39 and processing of the cathelin precursor to the antibacterial peptide LL-37 in granulocytes. Eur. J. Biochem. 238, 325.
8. Harboe, N.M.G., Ingild, A., 1983. Immunization, isolation of immunoglobulins and antibody titre determination. Scand. J. Immunol. 17, 345.
9. Kjeldsen, L., Bjerrum, O.W., Hovgaard, D., Johnsen, A.H., Sehested, M., Borregaard, N., 1992. Human neutrophil gelatinase: A marker for circulating blood neutrophils. Purification and quantitation by enzyme linked immunosorbent assay. Eur. J. Haematol. 49, 180.
10. Kjeldsen, L., Koch, C., Arnljots, K., Borregaard, N., 1996. Characterization of two ELISAs for NGAL, a newly described lipocalin in human neutrophils. J. Immunol. Methods 198, 155.
11. Laemmli, U.K., 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680.
12. Larrick, J.W., Michimasa, H., Balint, R.F., Lee, J., Zhong, J., Wright, S.C., 1995. Human CAP18: A novel antimicrobial lipopolysaccharide-binding protein. Infect. Immun. 63, 1291.
13. Larrick, J.W., Lee, J., Ma, S., Li, X., Francke, U., Wright, S.C., Balint, R.F., 1996. Structural, functional analysis and localization of the human CAP18 gene. FEBS Lett. 398, 74.
14. Lollike, K., Kjeldsen, L., Sengeløv, H., Borregaard, N., 1994a. Purification of lysozyme from human neutrophils, and development of an ELISA for quantification in cells and plasma. Leukemia 9, 206.
15. Lollike, K., Kjeldsen, L., Sengeløv, H., Borregaard, N., 1994b. Lysozyme in human neutrophils and plasma. A parameter of myelopoietic activity. Leukemia 9, 159.
16. Lollike, K., Sørensen, O., Bundgaard, J.R., Segal, A.W., Boyhan, A., Borregaard, N., 1995. An ELISA for grancalcin, a novel cytosolic calcium-binding protein present in leukocytes. J. Immunol. Methods 185, 1.
17. Ritonja, A., Kopitar, M., Jerala, R., Turk, V., 1989. Primary structure of a new cysteine proteinase inhibitor from pig leucocytes. FEBS Lett. 255, 211.
18. Schuster, R., 1988. Determination of amino acids in biological, pharmaceutical, plant, and food samples by automated precolumn derivatization and high-performance liquid chromatography. J. Chromatogr. 431, 271.
19. Towbin, H., Staehlin, T., Gordon, J., 1979. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Proc. Natl. Acad. Sci. USA 76, 4350.
20. Zanetti, M., Gennaro, R., Romeo, D., 1995. Cathelicidins: A novel protein family with a common proregion and a variable C-terminal antimicrobial domain. FEBS Lett. 374, 1.