-
2
-
-
0026599977
-
Reverse staining of sodium dodecylsulfate polyacrylamide gels by imidazole-zinc salts: Sensitive detection of unmodified proteins
-
Fernández-Patrón, C., Castellanos-Serra, L. & Rodríguez, P. Reverse staining of sodium dodecylsulfate polyacrylamide gels by imidazole-zinc salts: Sensitive detection of unmodified proteins. BioTechniques 12, 564-573 (1992).
-
(1992)
BioTechniques
, vol.12
, pp. 564-573
-
-
Fernández-Patrón, C.1
Castellanos-Serra, L.2
Rodríguez, P.3
-
3
-
-
1842686326
-
Reverse staining of biomolecules in electrophoresis gels: Analytical and micropreparative applications
-
Hardy, E. & Castellanos-Serra, L. Reverse staining of biomolecules in electrophoresis gels: Analytical and micropreparative applications. Anal. Biochem. 328, 1-13 (2004).
-
(2004)
Anal. Biochem
, vol.328
, pp. 1-13
-
-
Hardy, E.1
Castellanos-Serra, L.2
-
4
-
-
0035047599
-
Detection of biomolecules in electrophoresis gels with salts of imidazole and zinc II: A decade of research
-
Castellanos-Serra, L. & Hardy, E. Detection of biomolecules in electrophoresis gels with salts of imidazole and zinc II: A decade of research. Electrophoresis 22, 864-873 (2001).
-
(2001)
Electrophoresis
, vol.22
, pp. 864-873
-
-
Castellanos-Serra, L.1
Hardy, E.2
-
5
-
-
0031785395
-
Understanding the mechanism of the zinc-ion stains of biomacromolecules in electrophoresis gels: Generalization of the reverse staining technique
-
Fernández-Patrón, C. et al. Understanding the mechanism of the zinc-ion stains of biomacromolecules in electrophoresis gels: generalization of the reverse staining technique. Electrophoresis 19, 2398-2406 (1998).
-
(1998)
Electrophoresis
, vol.19
, pp. 2398-2406
-
-
Fernández-Patrón, C.1
-
7
-
-
34548861044
-
Sodium dodecyl sulfate-polyacrylamide gel electrophoresis
-
eds. Hamdan, M. & Righetti, P.G, Wiley Interscience, Hoboken, New Jersey
-
Righetti, P.G. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis. in Proteomics Today (eds. Hamdan, M. & Righetti, P.G.) 289-294 (Wiley Interscience, Hoboken, New Jersey, 2005).
-
(2005)
Proteomics Today
, pp. 289-294
-
-
Righetti, P.G.1
-
8
-
-
0032605255
-
-
ed. Link, A.J, Humana Press, Totowa, New Jersey
-
Courchesne, P.L. & Patterson, S.D. in Methods in Molecular Biology: 2-D Protein Gel Electrophoresis Protocols (ed. Link., A.J.) 487-511 (Humana Press, Totowa, New Jersey, 1998).
-
(1998)
Methods in Molecular Biology: 2-D Protein Gel Electrophoresis Protocols
, pp. 487-511
-
-
Courchesne, P.L.1
Patterson, S.D.2
-
9
-
-
0032619186
-
-
ed. Link, A.J, Humana Press, Totowa, New Jersey
-
Matsui, N.M., Smith-Beckerman, D.M. & Epstein, L.B. in Methods in Molecular Biology: 2-D Protein Gel Electrophoresis Protocols (ed. Link, A.J.) 307-312 (Humana Press, Totowa, New Jersey, 1998).
-
(1998)
Methods in Molecular Biology: 2-D Protein Gel Electrophoresis Protocols
, pp. 307-312
-
-
Matsui, N.M.1
Smith-Beckerman, D.M.2
Epstein, L.B.3
-
10
-
-
34548831185
-
-
2+ reverse staining of proteins in polyacrylamide gels in Proteomics Protocols on CD-ROM (ed. Walker, J.M.) Section 2.21 (Humana Press, Totowa, New Jersey, 1999).
-
2+ reverse staining of proteins in polyacrylamide gels in Proteomics Protocols on CD-ROM (ed. Walker, J.M.) Section 2.21 (Humana Press, Totowa, New Jersey, 1999).
-
-
-
-
11
-
-
0142208703
-
-
3rd edn, Wiley-VCH, Weinheim, Germany
-
Westermeier, R. & Naven, T. in Proteomics in Practice 3rd edn. (Wiley-VCH, Weinheim, Germany, 2002).
-
(2002)
Proteomics in Practice
-
-
Westermeier, R.1
Naven, T.2
-
12
-
-
0028836681
-
Protein reverse staining: High-efficiency microanalysis of unmodified proteins detected on electrophoresis gels
-
Fernández-Patrón, C. et al. Protein reverse staining: high-efficiency microanalysis of unmodified proteins detected on electrophoresis gels. Anal. Biochem. 224, 203-211 (1995).
-
(1995)
Anal. Biochem
, vol.224
, pp. 203-211
-
-
Fernández-Patrón, C.1
-
13
-
-
0026601551
-
Imidazol-SDS-Zn reverse staining of proteins in gels containing or not SDS and microsequence of individual unmodified electroblotted proteins
-
Ortiz, M.L., Calero, M., Fernández-Patrón, C., Castellanos-Serra, L. & Méndez, E. Imidazol-SDS-Zn reverse staining of proteins in gels containing or not SDS and microsequence of individual unmodified electroblotted proteins. FEBS Lett. 296, 300-304 (1992).
-
(1992)
FEBS Lett
, vol.296
, pp. 300-304
-
-
Ortiz, M.L.1
Calero, M.2
Fernández-Patrón, C.3
Castellanos-Serra, L.4
Méndez, E.5
-
14
-
-
0029902402
-
A procedure for protein elution from reverse stained polyacrylamide gels applicable at the low picomole level: An alternative route to the preparation of low abundance proteins for microanalysis
-
Castellanos-Serra, L., Fernández-Patrón, C., Hardy, E. & Huerta, V. A procedure for protein elution from reverse stained polyacrylamide gels applicable at the low picomole level: An alternative route to the preparation of low abundance proteins for microanalysis. Electrophoresis 17, 1564-1572 (1996).
-
(1996)
Electrophoresis
, vol.17
, pp. 1564-1572
-
-
Castellanos-Serra, L.1
Fernández-Patrón, C.2
Hardy, E.3
Huerta, V.4
-
15
-
-
0032213482
-
Elution of unmodified oligodeoxynucleotides from zinc-imidazole negatively stained polyacrylamide ges
-
Hardy, E. et al. Elution of unmodified oligodeoxynucleotides from zinc-imidazole negatively stained polyacrylamide ges. Anal. Biochem. 264, 127-129 (1998).
-
(1998)
Anal. Biochem
, vol.264
, pp. 127-129
-
-
Hardy, E.1
-
16
-
-
0032213482
-
Elution of lipopolysaccharides from polyacrylamide gels
-
Hardy, E., Pupo, E., Santana, H., Guerra, M. & Castellanos-Serra, L. Elution of lipopolysaccharides from polyacrylamide gels. Anal. Biochem. 264, 127-129 (1998).
-
(1998)
Anal. Biochem
, vol.264
, pp. 127-129
-
-
Hardy, E.1
Pupo, E.2
Santana, H.3
Guerra, M.4
Castellanos-Serra, L.5
-
17
-
-
0342905041
-
High-efficiency passive elution of bacterial lipopolysaccharides from polyacrylamide gels
-
Pupo, E., López, C.M., Alonso, M. & Hardy, E. High-efficiency passive elution of bacterial lipopolysaccharides from polyacrylamide gels. Electrophoresis 21, 526-530 (2000).
-
(2000)
Electrophoresis
, vol.21
, pp. 526-530
-
-
Pupo, E.1
López, C.M.2
Alonso, M.3
Hardy, E.4
-
18
-
-
0029075734
-
Single-step electrotransfer of reverse-stained proteins from sodium dodecyl sulfate-polyacrylamide gel onto reverse-phase minicartridge and subsequent desalting and elution with a conventional high-performance liquid chromatography gradient system for analysis
-
Fernández-Patrón, C. et al. Single-step electrotransfer of reverse-stained proteins from sodium dodecyl sulfate-polyacrylamide gel onto reverse-phase minicartridge and subsequent desalting and elution with a conventional high-performance liquid chromatography gradient system for analysis. Electrophoresis 16, 911-920 (1995).
-
(1995)
Electrophoresis
, vol.16
, pp. 911-920
-
-
Fernández-Patrón, C.1
-
19
-
-
0002672269
-
-
eds. Kamp, R.M, Kyriakidis, O, Choli-Papadodlulou, T, Springer, Heidelberg
-
Castellanos-Serra, L. et al. in Proteins and Proteome Analysis (eds. Kamp, R.M., Kyriakidis, O., Choli-Papadodlulou, T.) 29-52 (Springer, Heidelberg, 1999).
-
(1999)
Proteins and Proteome Analysis
, pp. 29-52
-
-
Castellanos-Serra, L.1
-
20
-
-
0031842514
-
-
Gevaert, K., Demol, H., Sklyarova, T., Vandekerckhove, J. & Houthaeve, T. Electrophoresis 19, 909-917 (1998).
-
(1998)
Electrophoresis
, vol.19
, pp. 909-917
-
-
Gevaert, K.1
Demol, H.2
Sklyarova, T.3
Vandekerckhove, J.4
Houthaeve, T.5
-
21
-
-
0030905461
-
Comparison of in-gel and on-membrane digestion methods at low to sub-pmol level for subsequent peptide and fragment-ion mass analysis using matrix-assisted laser-desorption/ionization mass spectrometry
-
Courchesne, P., Luethy, R. & Patterson, S.D. Comparison of in-gel and on-membrane digestion methods at low to sub-pmol level for subsequent peptide and fragment-ion mass analysis using matrix-assisted laser-desorption/ionization mass spectrometry. Electrophoresis 18, 369-381 (1997).
-
(1997)
Electrophoresis
, vol.18
, pp. 369-381
-
-
Courchesne, P.1
Luethy, R.2
Patterson, S.D.3
-
22
-
-
0034096536
-
Protein identification methods in proteomics
-
Gevaert, K. & Vandekerckhove, J. Protein identification methods in proteomics. Electrophoresis 21, 1145-1154 (2000).
-
(2000)
Electrophoresis
, vol.21
, pp. 1145-1154
-
-
Gevaert, K.1
Vandekerckhove, J.2
-
23
-
-
0030586928
-
Recovery of biologically active proteins detected with imidazole-SDS-zinc (reverse staining) on sodium dodecyl sulfate gels
-
Hardy, E. et al. Recovery of biologically active proteins detected with imidazole-SDS-zinc (reverse staining) on sodium dodecyl sulfate gels. Anal. Biochem. 240, 150-152 (1996).
-
(1996)
Anal. Biochem
, vol.240
, pp. 150-152
-
-
Hardy, E.1
-
24
-
-
0030741557
-
High yield elution of proteins from sodium dodecyl sulfate-polyacrylamide gels at the low-picomole level. Application to N-terminal sequencing of a scarce protein and to in solution biological activity analysis of on-gel renatured proteins
-
Castellanos-Serra, L.R., Fernández-Patrón, C., Hardy, E., Santana, H. & Huerta, V. High yield elution of proteins from sodium dodecyl sulfate-polyacrylamide gels at the low-picomole level. Application to N-terminal sequencing of a scarce protein and to in solution biological activity analysis of on-gel renatured proteins. J. Prot. Chem. 16, 415-419 (1997).
-
(1997)
J. Prot. Chem
, vol.16
, pp. 415-419
-
-
Castellanos-Serra, L.R.1
Fernández-Patrón, C.2
Hardy, E.3
Santana, H.4
Huerta, V.5
-
25
-
-
0032924359
-
Proteome analysis of polyacrylamide gel-separated proteins visualized by reversible negative staining using imidazole-zinc salts
-
Castellanos-Serra, L., Proenza, W., Huerta, V., Moritz R. & Simpson, R. Proteome analysis of polyacrylamide gel-separated proteins visualized by reversible negative staining using imidazole-zinc salts. Electrophoresis 20, 732-737 (1999).
-
(1999)
Electrophoresis
, vol.20
, pp. 732-737
-
-
Castellanos-Serra, L.1
Proenza, W.2
Huerta, V.3
Moritz, R.4
Simpson, R.5
-
26
-
-
0030029442
-
Structural analysis and identification of gel-purified proteins, available in the femtomole range, using a novel computer program for peptide sequence assignment, by matrix-assisted laser desorption ionization-reflectron time-of-ftight-mass spectrometry
-
Gevaert, K. et al. Structural analysis and identification of gel-purified proteins, available in the femtomole range, using a novel computer program for peptide sequence assignment, by matrix-assisted laser desorption ionization-reflectron time-of-ftight-mass spectrometry. Electrophoresis 17, 918-924 (1996).
-
(1996)
Electrophoresis
, vol.17
, pp. 918-924
-
-
Gevaert, K.1
-
27
-
-
0032940148
-
Mice immunization with gel electrophoresis-micropurified bacterial lipopolysaccharides
-
Pupo, E., Águila, A., Santana, H., Núñez, J.F. & Hardy, E. Mice immunization with gel electrophoresis-micropurified bacterial lipopolysaccharides. Electrophoresis 20, 458-461 (1999).
-
(1999)
Electrophoresis
, vol.20
, pp. 458-461
-
-
Pupo, E.1
Águila, A.2
Santana, H.3
Núñez, J.F.4
Hardy, E.5
-
28
-
-
0032603342
-
-
ed. Link, A.J, Humana Press, Totowa, New Jersey
-
Jensen, O.N., Wilm, M., Shevchenko, A. & Mann, M. in Methods in Molecular Biology: 2-D Protein Gel Electrophoresis Protocols (ed. Link, A.J.) 513-530 (Humana Press, Totowa, New Jersey, 1999).
-
(1999)
Methods in Molecular Biology: 2-D Protein Gel Electrophoresis Protocols
, pp. 513-530
-
-
Jensen, O.N.1
Wilm, M.2
Shevchenko, A.3
Mann, M.4
-
29
-
-
0031052205
-
Identification of low to subpicomolar quantities of electrophoretically separated proteins: Towards protein chemistry in the post-genome era
-
Patterson, S.D. Identification of low to subpicomolar quantities of electrophoretically separated proteins: Towards protein chemistry in the post-genome era. Biochem. Soc. Trans. 25, 255-262 (1997).
-
(1997)
Biochem. Soc. Trans
, vol.25
, pp. 255-262
-
-
Patterson, S.D.1
-
30
-
-
0031044318
-
Manual microcolumn chromatography for sample cleanup before mass spectrometry
-
Courchesne, P.L. & Patterson, S.D. Manual microcolumn chromatography for sample cleanup before mass spectrometry. Bio Techniques 22, 244-250 (1997).
-
(1997)
Bio Techniques
, vol.22
, pp. 244-250
-
-
Courchesne, P.L.1
Patterson, S.D.2
-
31
-
-
0031814739
-
Optimization of capillary chromatography ion trap-mass spectrometry for identification of gel-separated proteins
-
Courchesne, P.L. et al. Optimization of capillary chromatography ion trap-mass spectrometry for identification of gel-separated proteins. Electrophoresis 19, 956-967 (1998).
-
(1998)
Electrophoresis
, vol.19
, pp. 956-967
-
-
Courchesne, P.L.1
-
32
-
-
0002064031
-
Identification of proteins by mass spectrometry
-
ed. Rabilloud, T, Springer Verlag, Heidelberg
-
Corthals, G., Gygi, S., Aebersold, R. & Patterson, S.D. Identification of proteins by mass spectrometry. in Proteome Research: Two-dimensional Gel Electrophoresis and Identification Methods (ed. Rabilloud, T.) 197-231 (Springer Verlag, Heidelberg, 1999).
-
(1999)
Proteome Research: Two-dimensional Gel Electrophoresis and Identification Methods
, pp. 197-231
-
-
Corthals, G.1
Gygi, S.2
Aebersold, R.3
Patterson, S.D.4
-
33
-
-
0034079942
-
A simplified device for protein identification by microcapillary gradient liquid chromatography-tandem mass spectrometry
-
McGinley, M.D. et al. A simplified device for protein identification by microcapillary gradient liquid chromatography-tandem mass spectrometry. Electrophoresis 21, 1678-1684 (2000).
-
(2000)
Electrophoresis
, vol.21
, pp. 1678-1684
-
-
McGinley, M.D.1
-
34
-
-
0032935361
-
Analysis of the mouse proteome. (I) Brain proteins: Separation by two-dimensional electrophoresis and identification by mass spectrometry and genetic variation
-
Gauss, C., Kalkum, M., Lowe, M., Lehrach, H. & Klose, J. Analysis of the mouse proteome. (I) Brain proteins: Separation by two-dimensional electrophoresis and identification by mass spectrometry and genetic variation. Electrophoresis 20, 575-566 (1999).
-
(1999)
Electrophoresis
, vol.20
, pp. 575-566
-
-
Gauss, C.1
Kalkum, M.2
Lowe, M.3
Lehrach, H.4
Klose, J.5
-
35
-
-
0141482205
-
Linking mass spectrometry and stab-polyacrylamide gel electrophoresis by passive elution of lipopolysaccharides from reverse-stained gels: Analysis of gel-purified lipopolysaccharides from Haemophilus influenzae strain Rd
-
Gulin, S., Pupo, E., Schweda, E.K. & Hardy, E. Linking mass spectrometry and stab-polyacrylamide gel electrophoresis by passive elution of lipopolysaccharides from reverse-stained gels: Analysis of gel-purified lipopolysaccharides from Haemophilus influenzae strain Rd. Anal. Chem. 75, 4918-4924 (2003).
-
(2003)
Anal. Chem
, vol.75
, pp. 4918-4924
-
-
Gulin, S.1
Pupo, E.2
Schweda, E.K.3
Hardy, E.4
-
36
-
-
4344590801
-
Matrix-assisted laser desorption/ionization-time of flight-mass spectrometry of lipopolysaccharide species separated by slab-polyacrylamide gel electrophoresis: High-resolution separation and molecular weight determination of lipooligosaccharides from Vibrio fischeri strain HMK
-
Pupo, E., Phillips, N.J., Gibson, B.W., Apicella, M.A. & Hardy, E. Matrix-assisted laser desorption/ionization-time of flight-mass spectrometry of lipopolysaccharide species separated by slab-polyacrylamide gel electrophoresis: High-resolution separation and molecular weight determination of lipooligosaccharides from Vibrio fischeri strain HMK. Electrophoresis 25, 2156-2164 (2004).
-
(2004)
Electrophoresis
, vol.25
, pp. 2156-2164
-
-
Pupo, E.1
Phillips, N.J.2
Gibson, B.W.3
Apicella, M.A.4
Hardy, E.5
-
37
-
-
0028869865
-
Double staining of Coomassie blue stained gels by imidazole-zinc reverse staining: Sensitive detection of Coomassie blue undetected proteins
-
Fernández-Patrón, C., Hardy, E., Sosa, A., Seoane, J. & Castellanos-Serra, L. Double staining of Coomassie blue stained gels by imidazole-zinc reverse staining: Sensitive detection of Coomassie blue undetected proteins. Anal. Biochem. 224, 263-269 (1995).
-
(1995)
Anal. Biochem
, vol.224
, pp. 263-269
-
-
Fernández-Patrón, C.1
Hardy, E.2
Sosa, A.3
Seoane, J.4
Castellanos-Serra, L.5
-
38
-
-
0026618996
-
Preincubation with cysteine prevents modification of sulfhydryl groups in proteins by unreacted acrylamide in a gel
-
Chiari, M., Righetti, P.G., Negri, A., Ceciliani, F. & Ronchi, S. Preincubation with cysteine prevents modification of sulfhydryl groups in proteins by unreacted acrylamide in a gel. Electrophoresis 13, 882-884 (1992).
-
(1992)
Electrophoresis
, vol.13
, pp. 882-884
-
-
Chiari, M.1
Righetti, P.G.2
Negri, A.3
Ceciliani, F.4
Ronchi, S.5
-
39
-
-
0030904058
-
Immobilized pH gradient two-dimensional gel electrophoresis and mass spectrometric identification of cytokine-regulated proteins in ME-180 cervical carcinoma cells
-
Matsui, N.M. et al. Immobilized pH gradient two-dimensional gel electrophoresis and mass spectrometric identification of cytokine-regulated proteins in ME-180 cervical carcinoma cells. Electrophoresis 18, 409-417 (1997).
-
(1997)
Electrophoresis
, vol.18
, pp. 409-417
-
-
Matsui, N.M.1
-
40
-
-
0031813004
-
Two-dimensional electrophoresis of human placental mitochondria and protein identification by mass spectrometry: Toward a human mitochondrial proteome
-
Rabilloud, T. et al. Two-dimensional electrophoresis of human placental mitochondria and protein identification by mass spectrometry: toward a human mitochondrial proteome. Electrophoresis 19, 1006-1014 (1998).
-
(1998)
Electrophoresis
, vol.19
, pp. 1006-1014
-
-
Rabilloud, T.1
-
41
-
-
0029902322
-
Negative staining with zinc-imidazole of gel electrophoresis-separated nucleic acids by zinc-imidazole
-
Hardy, E. et al. Negative staining with zinc-imidazole of gel electrophoresis-separated nucleic acids by zinc-imidazole. Electrophoresis 17, 1537-1541 (1996).
-
(1996)
Electrophoresis
, vol.17
, pp. 1537-1541
-
-
Hardy, E.1
-
42
-
-
0035061674
-
Comparison of two electrophoresis-based DNA micropurification methods for molecular cloning
-
Hardy, E. et al. Comparison of two electrophoresis-based DNA micropurification methods for molecular cloning. Biotecnologia Aplicada 18, 29-32 (2001).
-
(2001)
Biotecnologia Aplicada
, vol.18
, pp. 29-32
-
-
Hardy, E.1
-
43
-
-
0032520818
-
Fast passive elution of DNA from zinc-imidazole negatively stained polyacrylamide gels
-
Castellanos-Serra, L., Hardy, E, & Sánchez, J.C. Fast passive elution of DNA from zinc-imidazole negatively stained polyacrylamide gels. Anal. Biochem. 257, 227-228 (1998).
-
(1998)
Anal. Biochem
, vol.257
, pp. 227-228
-
-
Castellanos-Serra, L.1
Hardy, E.2
Sánchez, J.C.3
-
44
-
-
0031031929
-
Sensitive reverse staining of bacterial lipopolysacharides on polyacrylamide gels by using zinc and imidazole salts
-
Hardy, E., Pupo, E., Castellanos-Serra, L., Reyes, J. & Fernández-Patrón, C. Sensitive reverse staining of bacterial lipopolysacharides on polyacrylamide gels by using zinc and imidazole salts. Anal. Biochem. 244, 28-32 (1997).
-
(1997)
Anal. Biochem
, vol.244
, pp. 28-32
-
-
Hardy, E.1
Pupo, E.2
Castellanos-Serra, L.3
Reyes, J.4
Fernández-Patrón, C.5
-
45
-
-
0021988172
-
Simplified method for silver staining of proteins in polyacrylamide gels and the mechanism of silver staining
-
Heukeshoven, J. & Dernick, R. Simplified method for silver staining of proteins in polyacrylamide gels and the mechanism of silver staining. Electrophoresis 6, 103-112 (1985).
-
(1985)
Electrophoresis
, vol.6
, pp. 103-112
-
-
Heukeshoven, J.1
Dernick, R.2
|