High yield elution of proteins from sodium dodecyl sulfate- polyacrylamide gels at the low-picomole level. Application to N-terminal sequencing of a scarce protein and to in-solution biological activity analysis of on-gel renatured proteins

Journal of Protein Chemistry

Volumn 16, Issue 5, 1997, Pages 415-419

  Castellanos Serra, Lila R ^a   Fernandez Patron, Carlos ^a   Hardy, Eugenio ^a   Santana, Hector ^a   Huerta, Vivian ^a  

^a CENTER FOR GENETIC ENGINEERING AND BIOTECHNOLOGY   (Cuba)

Author keywords

Passive elution; Protein detection; Protein micropurification; SDS PAGE; Trace enrichment

Indexed keywords

POLYACRYLAMIDE GEL;

CONFERENCE PAPER; PROTEIN ANALYSIS; PROTEIN DETERMINATION; PROTEIN PURIFICATION; PROTEIN STRUCTURE; STRUCTURE ANALYSIS; TECHNIQUE;

ACRYLIC RESINS; AMINO ACID SEQUENCE; BUFFERS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; DETERGENTS; HYDROGEN-ION CONCENTRATION; MEMBRANES, ARTIFICIAL; MICROCHEMISTRY; POLYVINYLS; PROTEIN DENATURATION; PROTEINS; SENSITIVITY AND SPECIFICITY; SEQUENCE ANALYSIS; SODIUM DODECYL SULFATE; SOLUTIONS; STAINING AND LABELING;

EID: 0030741557     PISSN: 02778033     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1026340923032     Document Type: Conference Paper

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