Structural analysis and identification of gel-purified proteins, available in the femtomole range, using a novel computer program for peptide sequence assignment, by matrix-assisted laser desorption ionization - Reflectron time-of-flight - mass spectrometry

Electrophoresis

Volumn 17, Issue 5, 1996, Pages 918-924

  Gevaert, Kris ^a   Verschelde, Jean Luc ^a   Puype, Magda ^a   Van Damme, Jozef ^a   Goethals, Marc ^a   De Boeck, Stefaan ^a   Vandekerckhove, Joël ^a  

^a DEPARTMENT OF PLANT SYSTEMS BIOLOGY   (Belgium)

Author keywords

Gel electrophoresis; Mass spectrometry; Peptide mass fingerprinting; Post source decay; Protein identification

Indexed keywords

DESORPTION; ELECTROPHORESIS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; IONIZATION; MASS SPECTROMETRY; PURIFICATION; SODIUM DODECYL SULFATE; SULFUR COMPOUNDS; PEPTIDES;

GEL ELECTROPHORESIS; MATCHINGS; MATRIX ASSISTED LASER DESORPTION IONIZATION; MATRIXASSISTED LASER DESORPTION-IONIZATION (MALDI); PEPTIDE MASS FINGERPRINTING; PEPTIDE SEQUENCES; POSTSOURCE DECAY; PROTEIN IDENTIFICATION; PURIFIED PROTEIN; REFLECTRONS;

PEPTIDES; MASS SPECTROMETRY;

ACTIN; BOVINE SERUM ALBUMIN;

AMINO ACID SEQUENCE; COMPUTER PROGRAM; CONFERENCE PAPER; GEL ELECTROPHORESIS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; MASS SPECTROMETRY; NONHUMAN; PROTEIN ANALYSIS; PROTEIN PURIFICATION; STAINING; TECHNIQUE;

EID: 0030029442     PISSN: 01730835     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (25)

1. Vandekerckhove, J., Bauw, G., Puype, M., Van Damme, J., Van Montagu, M., Eur. J. Biochem. 1985, 152, 9-19.
2. Celis, J. E., Rasmussen, H.-H., Leffers, H., Madsen, P., Honoré, R., Getser, B., Dejgaard, K., Vandekerckhove, J., FASEB J. 1991, 5, 2200-2208.
3. Vandekerckhove, J., Rider, M., Rasmussen, H.-H., De Boeck, S., Puype, M., Van Damme, J., Gesser, B., Celis, J., in: Imahori, K., Sakiyama, F. (Eds.), Methods in Protein Sequence Analysis, Plenum Press, New York 1993, pp. 11-19.
4. Rider, M. H., Puype, M., Van Damme, J., Gevaert, K., De Boeck, S., D'Alayer, J., Rasmussen, H.-H., Celis, J. E., Vandekerckhove, J., Eur. J. Biochem. 1995, 230, 258-265.
5. Gevaert, K., Rider, M. H., Puype, M., Van Damme, J., De Boeck, S., Vandekerckhove, J. in: Atassi, M. Z., Appella, E. (Eds.), Methods in Protein Structure Analysis, Plenum Press, New Yok 1995, pp. 15-26.
6. Henzel, W.J., Billecci, T. M., Stults, J. T., Wong, S. C., Grimley, C., Watanabe, C., Proc. Natl. Acid. Sci. USA 1993, 90, 5011-5015.
7. Mann, M., Højrup, P., Roepstorff, P., Biol. Mass Spectrom. 1993, 22, 338-345.
8. Pappin, D. J. C., Højrup, P., Bleasby, A. J., Curr. Biol. 1993, 3, 327-332.
9. Yates, J. R., Speicher, S., Griffin, P. R., Hunkapiller, T., Anal. Biochem. 1993, 214, 397-408.
10. Hess, D., Covey, T. C., Winz, R., Brownsey, R., Aebersold, R., Prot. Sci. 1993, 2, 1342-1351.
11. Eckerskorn, C., Strupat, K., Karas, M., Hillenkamp, F., Lottspeich, F., Electrophoresis 1992, 13, 664-665.
12. Mann, M., Wilm, M., Anal. Chem. 1994, 66, 4390-4399.
13. Kaufmann, R., Spengler, B., Lutzenkirchen, F., Rapid Commun. Mass Spectrom. 1993, 7, 902-910.
14. Spudich, J. A., Watt, S., J. Biol. Chem. 1971, 246, 4866-4871.
15. Gettemans, J., De Ville, Y., Waelkens, E., Vandekerckhove, J., J. Biol. Chem. 1995, 270, 2644-2651.
16. Matsudaira, P. J., Burgess, D. R., Anal. Biochem. 1978, 87, 386-396.
17. Fernandez-Patron, C., Calero, M., Collazo, P. R., Garcia, J. R., Madrazo, J., Musacchio, A., Soriano, F., Estrada, R., Frank, R., Castellanos-Serra, L. R., Mendez, E., Anal. Biochem. 1995, 224, 203-211.
18. Wilson, C. M., Methods Enzymol. 1983, 91, 236-247.
19. Neuhoff, V., Arold, N., Taube, D., Ehrhardt, W., Electrophoresis 1988, 9, 255-262.
20. Rosenfeld, J., Capdevielle, J., Guillemot, J. C., Ferrara, P., Anal. Biochem. 1992, 203, 173-179.
21. Beavis, R. C., Chaudhary, T., Chait, B. T., Organ. Mass Spectrom. 1992, 27, 156-158.
22. Jenö, P., Mini, T., Moes, S., Hintermann, E., Horst, M., Anal. Biochem. 1995, 224, 75-82.
23. Griffin, P. R., MacCoss, M. J., Eng, J. K., Blevins, R. A., Aaronson, J. S., Yates, J. R., Rapid Commun. Mass Spectrom. 1995, 9, 1546-1551.
24. Lombard-Platet, G., Jalinot, P., Biotechniques 1993, 15, 668-670.
25. Yates, J. R., Eng, J. K. McCormack, A. L., Schieltz, D., Anal. Chem. 1995, 67, 1426-1436.