High-efficiency passive elution of bacterial lipopolysaccharides from polyacrylamide gels

Electrophoresis

Volumn 21, Issue 3, 2000, Pages 526-530

  Pupo, Elder ^a   López, Cruz Matilde ^a   Alonso, Mabel ^a   Hardy, Eugenio ^a  

^a CENTER FOR GENETIC ENGINEERING AND BIOTECHNOLOGY   (Cuba)

Author keywords

Gel electrophoresis; Lipopolysaccharides; Purification; Silver staining; Zinc staining

Indexed keywords

ELECTROPHORESIS; ESCHERICHIA COLI; ORGANIC SOLVENTS; POLYSACCHARIDES; RECOVERY; SILVER; SOAPS (DETERGENTS); SULFUR COMPOUNDS; ZINC;

GEL ELECTROPHORESIS; HIGHER EFFICIENCY; IMIDAZOL; LIPOPOLYSACCHARIDES; POLYACRYLAMIDE GELS; SILVER STAINING; SIMPLE++; SODIUM DODECYL SULPHATE; TRIETHYLAMINES; ZINC STAINING;

SODIUM DODECYL SULFATE;

ACETONITRILE; BACTERIUM LIPOPOLYSACCHARIDE; DEOXYCHOLATE SODIUM; DETERGENT; DODECYL SULFATE SODIUM; IMIDAZOLE; ORGANIC SOLVENT; POLYACRYLAMIDE GEL; TRIETHYLAMINE; TRITON X 100; WATER; ZINC;

ARTICLE; DIFFUSION; DILUTION; ESCHERICHIA COLI; EVAPORATION; LIMULUS LYSATE TEST; PARTICLE SIZE; POLYACRYLAMIDE GEL ELECTROPHORESIS; PURIFICATION; SERRATIA MARCESCENS; VIBRIO CHOLERAE;

EID: 0342905041     PISSN: 01730835     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1522-2683(20000201)21:3<526::AID-ELPS526>3.0.CO;2-9     Document Type: Article

References (20)

1. Scopes, R. K., Biotechnol. Appl. Biochem. 1996, 23, 197-204.
2. Patterson, S. D., Thomas, D., Bradshaw, R. A., Electrophoresis 1996, 17, 877-891.
3. Hardy, E., Pupo, E., Castellanos-Serra, L., Reyes, J., Fernández-Patrón, C., Anal. Biochem. 1997, 244, 28-32.
4. Fernández-Patrón, C., Castellanos-Serra, L., Hardy, E., Guerra, M., Estévez, E., Mehl, E., Frank, R. W., Electrophoresis 1998, 19, 2398-2406.
5. Hardy, E., Pupo, E., Santana, H., Guerra, M., Castellanos-Serra, L., Anal. Biochem. 1998, 259, 162-165.
6. Aurell, C. A., Wistrom, A. O., J. Biol. Chem. 1988, 263, 11971-11976.
7. Westphal, O., Jann, K., Methods Carbohydr. Chem. 1965, 5, 83-91.
8. Karkhanis, Y. D., Zeltner, J. Y., Jackson, J. J., Carlo, D. J., Anal. Biochem. 1978, 85, 595-601.
9. Tsai, C. M., Frasch, C. E., Anal. Biochem. 1982, 119, 115-119.
10. Peterson, A. A., McGroarty, E. J., J. Bacteriol. 1975, 121, 455-464.
11. Galanos, C., Luderitz, O., Eur. J. Biochem. 1975, 54, 603-610.
12. Tanamoto, K., Azumi, S., Haishima, Y., Kumada, H., Umemoto, T., J. Immunol. 1997, 158, 4430-4436.
13. Gibson, B. W., Engstrom, J. J., John, C. M., J. Am. Soc. Mass Spectrom. 1997, 8, 645-658.
14. Tsai, C., Chen, W., Balakonis, P., Glycoconjugate J. 1995, 12, 562.
15. Logan, S. M., Trust, T. J., Infect. Immun. 1984, 45, 210-216.
16. Dooley, J. S. G., Lallier, R., Shaw, D. H., Trust, T. J., J. Bacteriol. 1985, 164, 263-269.
17. Sandbulte, J., TerWee, J., Wigington, K., Sabara, M., Vet. Microbiol. 1996, 48, 269-282.
18. Kim, J. S., Reuhs, B. L., Rahman, M. M., Ridley, B., Carlson, R. W., Glycobiology 1996, 6, 433-437.
19. Suda, Y., Kirikae, T., Shiyama, T., Yasukochi, T., Kirikae, F., Nakano, M., Rietschel, E. T., Kusumoto, S., Biochem. Biophys. Res. Commun. 1995, 210, 678-685.
20. Sánchez, P. L., Viñas, M., Microbios 1998, 93, 55-68.