Comparison of in-gel and on-membrane digestion methods at low to sub-pmol level for subsequent peptide and fragment-ion mass analysis using matrix-assisted laser-desorption/ionization mass spectrometry

Electrophoresis

Volumn 18, Issue 3-4, 1997, Pages 369-381

  Courchesne, Paul L ^a   Luethy, Roland ^b   Patterson, Scott D ^a,b  

^a Protein Structure   (United States)

^b AMGEN INC   (United States)

Author keywords

Expressed sequence tag; Fragment ion searching; Mass spectrometry; Protein electrophoresis; Protein identification

Indexed keywords

CARBONIC ANHYDRASE; CASEIN; DESORPTION; ELECTROPHORESIS; IONS; MASS SPECTROMETERS; MATRIX ALGEBRA; MEMBRANES; PEPTIDES;

DIGESTION METHOD; EXPRESSED SEQUENCE TAGS; FRAGMENT IONS; FRAGMENT-ION SEARCHING; GEL-SEPARATED PROTEINS; MATRIX-ASSISTED LASER DESORPTION IONIZATION MASS SPECTROMETRY; NON-REDUNDANT; PEPTIDES IONS; PROTEIN ELECTROPHORESIS; PROTEIN IDENTIFICATION;

MASS SPECTROMETRY;

CARBONATE DEHYDRATASE II; GLYCOGEN PHOSPHORYLASE B; MYOGLOBIN;

AMINO ACID SEQUENCE; COMPUTER PROGRAM; CONFERENCE PAPER; DATA BASE; MASS SPECTROMETRY; MICROANALYSIS; PEPTIDE MAPPING; PROTEIN ANALYSIS; PROTEIN DEGRADATION; PROTEIN ELECTROPHORESIS; SILVER STAINING; TECHNIQUE;

EID: 0030905461     PISSN: 01730835     EISSN: None     Source Type: Journal    
DOI: 10.1002/elps.1150180311     Document Type: Conference Paper

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