Manual microcolumn chromatography for sample cleanup before mass spectrometry

BioTechniques

Volumn 22, Issue 2, 1997, Pages 244-250

  Courchesne, Paul L ^a   Patterson, Scott D ^a  

^a AMGEN INC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; COLUMN CHROMATOGRAPHY; COST EFFECTIVENESS ANALYSIS; CRYSTALLIZATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; IONIZATION; MASS SPECTROMETRY; PROTEIN ANALYSIS; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY;

EID: 0031044318     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/97222bm10     Document Type: Article

References (14)

1. Beavis, R.C. and B.T. Chait. 1990. Rapid, sensitive analysis of protein mixtures by mass spectrometry. Proc. Natl. Acad. Sci. USA 87:6873-6877.
2. Billeci, T.M. and J.T. Stults. 1993. Tryptic mapping of recombinant proteins by matrix-assisted laser desorption/ionization mass spectrometry. Anal. Chem. 65:1709-1716.
3. Bornsen, K.O., M.D. Mohr and H.M. Widmar. 1995. Ion exchange and purification of carbohydrates on a Nafion® membrane as a new sample pretreatment for matrix-assisted laser desorption/ionization mass spectrometry. Rapid Commun. Mass Spectrom. 9:1031-1034.
4. Hutchens, T.W. and T.-T. Yip. 1993. New desorption strategies for the mass spectrometric analysis of macromolecules. Rapid Commun. Mass Spectrom. 7:576-580.
5. Mock, K.K., C.W. Sutton and J.S. Cottrell. 1992. Sample immobilization protocols for matrix-assisted laser-desorption mass spectrometry. Rapid Commun. Mass Spectrom. 6:233-238.
6. Moritz, R., J. Eddes, H. Ji, G.E. Reid and R.J. Simpson. 1995. Rapid separation of proteins and peptides using conventional silica-based supports: identification of 2-D gel proteins following in-gel proteolysis, p. 311-319. In J.W. Crabb (Ed.), Techniques in Protein Chemistry VI. Academic Press, San Diego.
7. Ortiz, M.L., M. Calero, C.F. Patron, L. Castellanos and E. Mendez. 1992. Imidazole-SDS-Zn reverse staining of proteins in gels containing or not SDS and microsequence of individual unmodified electroblotted proteins. FEBS Lett. 296:300-304.
8. Patterson, S.D. 1995. Matrix-assisted laser-desorption/ionization mass spectrometric approaches for the identification of gel-separated proteins in the 5-50 pmol range. Electrophoresis 16:1104-1114.
9. Patterson, S.D. and V. Katta. 1994. Prompt fragmentation of disulfide-linked peptides during matrix-assisted laser desorption ionization mass spectrometry. Anal. Chem. 66:3727-3732.
10. Shevchenko, A., M. Wilm, O. Vorm and M. Mann. 1996. Mass-spectrometric sequencing of proteins from silver-stained polyacrylamide gels. Anal. Chem. 68:850-858.
11. Vorm, O., P. Roepstorff and M. Mann. 1994. Improved resolution and very high-sensitivity in MALDI TOF of matrix surfaces made by fast evaporation. Anal. Chem. 66:3281-3287.
12. Xiang, F. and R.C. Beavis. 1994. A method to increase contaminant tolerance in protein matrix-assisted laser desorption/ionization by the fabrication of thin protein-doped polycrystalline films. Rapid Commun. Mass Spectrom. 8:199-204.
13. Zaluzec, E.J., D.A. Gage, J. Allison and J.T. Watson. 1994. Direct matrix-assisted laser-desorption ionization mass-spectrometric analysis of proteins immobilized on nylon-based membranes. J. Am. Soc. Mass Spectrom. 5:230-237.
14. Zhang, H., P.E. Andren and R.M. Caprioli. 1995. Micro-preparation procedure for high-sensitivity matrix-assisted laser desorption ionization mass spectrometry. J. Mass Spectrom. 30:1768-1771.