A procedure for protein elution from reverse-stained polyacrylamide gels applicable at the low picomole level: An alternative route to the preparation of low abundance proteins for microanalysis

Electrophoresis

Volumn 17, Issue 10, 1996, Pages 1564-1572

  Castellanos Serra, Lila R ^a   Fernandez Patron, Carlos ^a   Hardy, Eugenio ^a   Huerta, Vivian ^a  

^a CENTER FOR GENETIC ENGINEERING AND BIOTECHNOLOGY   (Cuba)

Author keywords

Passive elution; Protein detection; Protein micropurification; Sodium dodecyl; Sulfate polyacrylamide gel electrophoresis; Trace enrichment

Indexed keywords

AMINO ACIDS; ELECTROPHORESIS; ESCHERICHIA COLI; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; SODIUM DODECYL SULFATE; SULFUR COMPOUNDS; ZINC COMPOUNDS;

ALTERNATIVE ROUTES; HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY; LOW-ABUNDANCE PROTEINS; PASSIVE ELUTION; POLYACRYLAMIDE GELS; PROTEIN DETECTION; PROTEIN MICROPURIFICATION; SODIUM DODECYL; SULPHATE-POLYACRYLAMIDE GEL ELECTROPHORESIS; TRACE ENRICHMENT;

PROTEINS;

ARTICLE; COMPLEX FORMATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MICROANALYSIS; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN ANALYSIS; PROTEIN PURIFICATION; QUANTITATIVE ASSAY; SEQUENCE ANALYSIS; TECHNIQUE; ULTRAVIOLET SPECTROPHOTOMETRY;

EID: 0029902402     PISSN: 01730835     EISSN: None     Source Type: Journal    
DOI: 10.1002/elps.1150171012     Document Type: Article

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