Determination of silencing potency of synthetic and RNase III-generated siRNA using a secreted luciferase assay

BioTechniques

Volumn 42, Issue 5, 2007, Pages 599-606

  Morlighem, Jean Étienne ^a   Petit, Céline ^a   Tzertzinis, George ^a  

^a NEW ENGLAND BIOLABS   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CELL CULTURE; CONCENTRATION (PROCESS); ENZYME ACTIVITY; GENETIC ENGINEERING; MEDICAL APPLICATIONS; PHYSIOLOGY;

ENZYMATIC COMPLEX; MICROTITER PLATES; REVERSE TRANSCRIPTION;

RNA;

LUCIFERASE; RIBONUCLEASE III; SMALL INTERFERING RNA;

ANIMAL CELL; ARTICLE; CELL CULTURE; CONTROLLED STUDY; ENZYME ASSAY; ENZYME RELEASE; GENE SILENCING; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; LUMINESCENCE; MICROTITER PLATE ASSAY; NONHUMAN; REPORTER GENE; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA INTERFERENCE; RNA STRUCTURE; RNA SYNTHESIS; WESTERN BLOTTING;

ANIMALS; BIOLOGICAL ASSAY; CERCOPITHECUS AETHIOPS; COS CELLS; GENE EXPRESSION REGULATION; GENES, REPORTER; HELA CELLS; HUMANS; LUCIFERASES; REPRODUCIBILITY OF RESULTS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RIBONUCLEASE III; RNA INTERFERENCE; RNA, SMALL INTERFERING; TUMOR SUPPRESSOR PROTEIN P53;

GAUSSIA PRINCEPS (T. SCOTT, 1894);

EID: 34250633692     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/000112444     Document Type: Article

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