RNAi gene silencing using cerasome as a viral-size siRNA-carrier free from fusion and cross-linking

Bioorganic and Medicinal Chemistry Letters

Volumn 17, Issue 14, 2007, Pages 3935-3938

  Matsui, Kazuki ^a   Sasaki, Yoshihiro ^b   Komatsu, Takayoshi ^b   Mukai, Masaru ^b   Kikuchi, Jun ichi ^b   Aoyama, Yasuhiro ^a  

^a KYOTO UNIVERSITY   (Japan)

^b NARA INSTITUTE OF SCIENCE AND TECHNOLOGY   (Japan)

Author keywords

Cerasome; Liposome; RNA; RNA interference; siRNA delivery

Indexed keywords

CERASOME; DOUBLE STRANDED DNA; DOUBLE STRANDED RNA; DRUG CARRIER; FIREFLY LUCIFERASE; LIPOSOME; PLASMID DNA; SMALL INTERFERING RNA; UNCLASSIFIED DRUG;

ARTICLE; COMPLEX FORMATION; CONTROLLED STUDY; DNA CROSS LINKING; GEL ELECTROPHORESIS; GENE SILENCING; HELA CELL; HUMAN; HUMAN CELL; LIPOSOMAL GENE DELIVERY SYSTEM; MATERIAL COATING; PARTICLE SIZE; RNA INTERFERENCE; RNA SEQUENCE;

BASE SEQUENCE; GENE SILENCING; LIPOSOMES; PLASMIDS; RNA INTERFERENCE; RNA, SMALL INTERFERING;

EID: 34250379038     PISSN: 0960894X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bmcl.2007.04.097     Document Type: Article

References (39)

1. Felgner P.L., Gadek T.R., Holm M., Roman R., Chan H.W., Wenz M., Northrop, Ringold G.M., and Danielson M. Proc. Natl. Acad. Sci. U.S.A. 84 (1987) 7413
2. In: Kabanov A.V., Felgner P.L., and Seymour L.W. (Eds). Self-Assembling Complexes for Gene Delivery. From Laboratory to Clinical Trial (1998), Wiley, Chichester
3. Behr J.-P. Acc. Chem. Res. 26 (1993) 274
4. Kabanov A.V., and Kabanov V.A. Bioconjugate Chem. 6 (1995) 7
5. Papahadjopoulos D., Hui S., Vail W.J., and Poste G. Biochim. Biophys. Acta 448 (1976) 245
6. Papahadjopoulos D., Poste G., and Vail W.J. Methods Membr. Biol. 10 (1979) 1
7. Ichikawa S., and Walde P. Langmuir 20 (2004) 941
8. Rupert L.A.M., Hoekstra D., and Engberts J.B.F.N. J. Am. Chem. Soc. 107 (1985) 2628
9. Weisman S., Hirsch-Lerner D., Barenholz Y., and Talmon Y. Biophys. J. 87 (2004) 609
10. Matsui K., Sando S., Sera T., Aoyama Y., Sasaki Y., Komastu T., Terashima T., and Kikuchi J. J. Am. Chem. Soc. 128 (2006) 3114
11. Sasaki Y., Matsui K., Aoyama Y., and Kikuchi J. Nat. Protocols 1 (2006) 1227
12. For polymer-coating approach to sterically stabilized liposomes, see:. Garbuzenko O., Zalipsky S., Qazen M., and Barenholz Y. Langmuir 21 (2005) 2560
13. Barenholz Y. Curr. Opin. Colloid Interface Sci. 6 (2001) 66
14. For examples of lipid-based siRNA delivery, see:. Elbashir S.M., Harborth J., Lendeckel W., Yalcin A., Weber K., and Tuschl T. Nature 411 (2001) 494
15. Spagnou S., Miller A.D., and Keller M. Biochemistry 43 (2004) 13348
16. Yano J., Hirabayashi K., Nakagawa S., Yamaguchi T., Nogawa M., Kashimori I., Naito H., Kitagawa H., Ishiyama K., Ohgi T., and Irimura T. Clin. Cancer Res. 15 (2004) 7721
17. t = 3. After incubation for 6 h at 37 °C, the medium was replaced by 100 μL of fresh DMEM containing 10% FCS, and the cells were further incubated for 48 h. Luciferase assay was performed by the chemiluminescence method using a Lumat LB5907 luminometer (Berthold Detection System) and the dual luciferase assay kit (Promega).
18. The DLS size distribution profile is shown in Supplementary data.
19. A fluorescence study for the resistance of cerasome against siRNA-induced fusion is shown in Supplementary data.
20. Fire A. Trends Genet. 15 (1999) 358
21. Sharp P.A. Genes Dev. 15 (2001) 485
22. Tuschl T. Chem. Biochem. 2 (2001) 239
23. Hannon G.J. Nature 418 (2002) 244
24. Hammond S.M., Bernstein E., Beach D., and Hannon G.J. Nature 404 (2000) 293
25. Hammond S.M., Boettcher S., Caudy A.A., Kobayashi R., and Hannon G.J. Science 293 (2001) 1146
26. firefly.
27. For the activity of lipofectamine 2000 as an siRNA carrier under similar conditions, see:. Matsui H., Horiuchi S., Sando S., Sera T., and Aoyama Y. Bioconjugate Chem. 17 (2006) 132
28. DsRed2 and lipid 1 at N/P = 2. After washing with PBS(-), the cells were dissolved in 25 μL of PLB (passive lysis buffer, Promega). The resulting solution was divided into two parts; one (2.5 μL) was analyzed for the total proteins by the Bradford assay (Bio-Rad Laboratories) and the other (20 μL) for the fluorescence intensity at 580 nm (excitation at 563 nm) after dilution with 380 μL of PBS(-).
29. DsRed2 = 252 ng are shown in Supplementary data.
30. Ref. 2a, Chapter 10, pp. 197-218.
31. Boussif O., Leozoulal'h F., Zanta M.A., Mergny M.D., Sherman D., Demeniex B., and Behr J.-P. Proc. Natl. Acad. Sci. U.S.A. 92 (1995) 7297
32. Rensen P.C., Sliedregt L.A., Ferns M., Kieveit E., van Rossenberg S.M., van Leeuwen S.H., van Berkel T.J., and Biessen E.A. J. Biol. Chem. 276 (2001) 37577
33. Osaki F., Kanamori T., Sando S., Sera T., and Aoyama Y. J. Am. Chem. Soc. 126 (2004) 6520
34. While factors other than size may also come into play, the better performance of the cerasome system could not be explained by the charge factor. A zeta potential study is shown in Supplementary data.
35. Brummelkamp T.R., Bernards R., and Agami R. Science 296 (2002) 550
36. Lee N.S., Dohjima T., Bauer G., Li H., Li M.-J., Ehsani A., Salvaterra P., and Rossi J. Nat. Biotechnol. 20 (2002) 500
37. Paul C.P., Good P.D., Winer I., and Engelke D.R. Nat. Biotechnol. 20 (2002) 505
38. Yu J.-Y., DeRuiter S.L., and Turner D.L. Proc. Natl. Acad. Sci. U.S.A. 99 (2002) 6047
39. Paddison P.J., Caudy A.A., Bernstein E., Hannon G.J., and Conklin D.S. Genes Dev. 16 (2002) 948